scholarly journals Pharmacological Evaluation and Antifertility Activity ofJatropha gossypifoliain Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sachin Jain ◽  
Gajendra Pratap Choudhary ◽  
Dinesh Kumar Jain
Keyword(s):  
Ethinyl Estradiol ◽  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Vehicle Control ◽  
Female Rats ◽  
Control Group ◽  
Traditional Use ◽  
Vehicle Control Group ◽  
Jatropha Gossypifolia ◽  
Female Wistar Rats

Objectives. Pharmacological and antifertility activity evaluation ofJatropha gossypifoliain rats.Methods. The antifertility activity of the extracts ofJatropha gossypifoliain rats was evaluated using two experimental animal models. Estrogenic activity was evaluated in immature female rats using ethinyl estradiol as standard. Anti-implantation and early abortifacient activity was performed in female Wistar rats by determining the number of implantations and implantation resorptions.Results. In estrogenic activity evaluations, the ethanolic and aqueous extracts offered significant estrogen-like activity at 400 mg kg−1p.o. by increasing the uterine weight compared to vehicle control group. Ethanolic extract (400 mg kg−1, p.o.) treatment significantly decreased the number of implants and increased the number of resorptions compared to vehicle control group.Conclusion. The results of the present study provide the evidence of the anti-fertility activity ofJatropha gossypifoliaas claimed in the traditional use. The results are consistent with the literature reports related to the antifertility effect of flower extracts ofJatropha gossypifolia.

A Teratological Evaluation and Postnatal Behavioral Screen of KF-868 In Rats

1985 ◽  
Vol 4 (1) ◽  
pp. 91-110 ◽  
Author(s):  
A. M. Hoberman ◽  
W. M. Weatherholtz ◽  
R. S. Durloo
Keyword(s):  
Body Weight ◽  
Vehicle Control ◽  
Female Rats ◽  
Reproductive Capacity ◽  
Control Group ◽  
Dosage Group ◽  
Vehicle Control Group ◽  
High Dosage Group ◽  
Significant Difference ◽  
Body Weights

The effects of a new experimental drug, KF-868, were investigated after administration to pregnant Sprague-Dawley rats at 0(vehicle), 0.1, 2.0, and 40.0 mg/kg per day during Days 7 through 17 of gestation by examination of term fetuses and naturally delivered offspring. Pregnant rats administered 0.1, 2.0, and 40.0 mg/kg per day gained significantly more weight during the dosage period than did the vehicle control group. Treatment-related physical signs, bloody crust on nose and stains on fur, were observed in the high dosage group. Fetal viability was significantly increased, and resorptions were significantly decreased for the mid and high dosage groups, when compared with the control group. Average fetal body weights for cesarean-delivered fetuses were less for the 40.0 mg/kg per day dosage groups than for the vehicle control group. Visceral and skeletal evaluations of fetuses revealed no difference between the control and test groups. Percent survival of pups was significantly less for the high dosage group than for the control group. Average rat body weights prior to mating for the high dosage group were generally less than for the control group. All physical and functional developmental values were comparable among the control and test groups. Evaluation of postweaning parameters of pups revealed no significant difference in sex maturation, behavior (open-field and water maze), and reproductive capacity. Average body weight gains during the 9-week growth period before mating were significantly less for the 40.0 mg/kg per day dosage group F1 generation female rats. Toxicity in fetuses and offspring was observed only at the highest dosage level. Dosage-dependent, significant increases in maternal body weight gain, as compared with control values, occurred for doses in the 3 KF-868-administered groups. These results indicate that 0.1 and 2.0 mg/kg per day dosages of KF-868 were not lethal and did not produce any adverse effects on the morphological or functional development of offspring. Toxicity was evident in offspring and fetuses of dams administered 40.0 mg/kg per day KF-868, 40,000 times as high as the daily therapeutic dose.

Diosgenin does not express estrogenic activity: a uterotrophic assay

2014 ◽  
Vol 92 (4) ◽  
pp. 292-298 ◽  
Author(s):  
Ivana Medigović ◽  
Nataša Ristić ◽  
Jasmina Živanović ◽  
Branka Šošić-Jurjević ◽  
Branko Filipović ◽  
...  
Keyword(s):  
Estrogen Receptor Alpha ◽  
Estrogenic Activity ◽  
Female Rats ◽  
Control Group ◽  
Immunohistochemical Expression ◽  
Vehicle Control Group ◽  
Immature Rat ◽  
Intact Control ◽  
Uterotrophic Assay ◽  
Wet Weight

This study assessed the effects of diosgenin on estrogenic activity using a uterotrophic assay. Immature female rats received diosgenin orally at doses of 200, 100, or 20 mg/kg body mass; and 17α ethynylestradiol at doses of 1 or 0.3 μg/kg, daily, for 3 consecutive days from day 19 to day 21. Controls were distributed among 2 groups: an intact control group and a vehicle control group. Animals were sacrificed 24 h after the last application of diosgenin, estradiol, or vehicle (22nd day of life). Uterine wet weight, stereological and histomorphometrical changes, immunohistochemical expression of estrogen receptor alpha (ERα), progesterone receptor (PR), and the expression of lactoferrin (LF) were examined. Diosgenin did not affect the uterine wet weight, epithelium height, volume densities of endometrium, endometrial epithelia, number of endometrial glands, or histological appearance of vaginal epithelia. ERα, PR, and LF immunostaining intensity were not altered in the animals that received diosgenin. High-potency reference ER agonist 17α-ethynylestradiol induced a significant increase in all of the measured parameters, and as expected, decreased ERα immunostaining intensity. Based on these data, it can be concluded that diosgenin, at doses of 20–200 mg/kg, did not act as an estrogen agonist in the immature rat uterotrophic assay.

scholarly journals Estrogenic Effect of Bambusa arundinacea, Trichosanthes dioica and Punica granatum on Rats

2022 ◽  
Author(s):  
Akanksha Awasthi ◽  
Mamta F. Singh ◽  
Saurabh Sharma
Keyword(s):  
Wistar Rats ◽  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Mature Female ◽  
Female Rats ◽  
Vaginal Opening ◽  
Estrogenic Effect ◽  
Female Wistar Rats ◽  
Trichosanthes Dioica

Background: Phytoestrogens have recently become a hot topic among scientists. Phytoestrogens’ estrogen-like properties have led to their widespread use in the reproductive system. The aim of this research was to see whether the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum had any estrogenic activity in female wistar rats. Methods: In female wistar rats, the estrogenic effect was studied using a uterotropic assay, vaginal cytology and vaginal opening. In ovariectomized immature and mature female wistar rats, a 400 mg/kg body weight (b.w.) dose of ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum was given. Result: When compared to ovariectomized control rats, the uterine wet weight increased significantly. The estrogen-treated rats had only cornified epithelial cells, indicating the existence of oestrogen, as well as 100% vaginal opening. At 400 mg/kg b.w., the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum demonstrated promising estrogenic activity, as evidenced by uterotropic assays, vaginal opening measurements and histopathological changes. As a result of this research, it’s possible to infer that the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum play an important role in estrogenic activity in female rats.

scholarly journals SOME IMMUNE FACTORS AND HORMONES DETERMINED IN FEMALE ALBINO RATS INDUCED WITH INFERTILITY AND ADMINISTERED WITH ANTHOCLIESTA VOGELII

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Olugbenga Oladimeji
Keyword(s):  
Test Group ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Albino Rats ◽  
Traditional Use ◽  
Clusters Of Differentiation ◽  
Automated Technique

Anthocleista vogelii Planch, phyto-constituents was evaluated and the plant leave extracts investigated on the claims of the traditional medicine practitioners of its usage as fertility enhancer in females. Ethanolic extract of Anthocleista vogelii were administered orally for 14 days to female albino rats placed in different groups. First, temporary infertility was induced with Micronor (norethisterone) or Nacetylcysteine (NAC) given orally to some rats, for seven (7) days prior to other treatment. The rats were sacrificed after the completion of extract administration. The absolute counts of clusters of differentiation CD4+ and CD8+ was performed on the blood samples using the Becton Dickinson’s (BD) FACS Count Automated technique. Hormonal analysis was performed on sera obtained from the experimental animals using commercial standard enzyme-linked immunosorbent assay kit. The extract was found to possess Anthraquinones, Terpenoids, Flavonoids, Saponins, Alkaloids, Phenols and Phytosterols . The obtained results of the test group compared with control showed a statistically significant decrease (P<0.05) in CD4+ and CD8+ counts cells and Prolactin, testosterone respectively. The results showed a significant increase of estradiol, leutinizing hormone, in the female rats in the control group compared to extract treated group. The result also suggested that Anthocleista vogelii may have a role in creating the environment required for enhancing pregnancy by decreasing ratio of CD4+ and CD8+ linked Th1 andTh2 cytokines production activation. Estradiol, luetinising hormone, concentration therefore support the claims on the traditional use of Anthocliesta vogelii that it enhance fertility in female.

scholarly journals Estrogenic Activity of Mahoni Seed Ethanolic Extract [Swietenia mahogany (L.) Jacq] on Uterus Weight, Bone Density and Mamae Gland Proliferation on Ovariectomized Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 75
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Rosa Gloria Sitanggang ◽  
Robbani Syahfitri Angkat
Keyword(s):  
Bone Density ◽  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Group 2 ◽  
Post Hoc

Menopause is a hypoestrogenic condition due to decreased function of the ovary. During menopause there is no reserved ovum in the ovary, as a result the synthesis of estrogen by the follicles does not take place. Deficiency of estrogen can lead to discomfort and decrease in the women quality of life. Therefore, supplements from natural resources to reduce menopausal symptoms will be needed. The objectives of the study were to determine the effect of mahogany seeds ethanolic extract (MSEE) on the development of uterus, bone density, and mammae gland proliferation on ovariectomized rats. Extract was made by maceration using 96% ethanol as the solvent, then the study of estrogenic effect was carried out on 30 female rats which were divided into 6 groups. Group 1 (normal control), group 2 (positive control) given estradiol dose of 0.18 mg/kg body weight (BW), group 3 (negative control) given Na-CMC 1% and group 4, 5, 6 given MSEE orally for 14 consecutive days with doses of 50, 100, 200 mg/kg BW. Data were analysed using ANOVA then continued with Tukey HSD Post Hoc test to see the differences between the treatments. The results of the study showed that MSEE was able to increase the weight of the uterus, the length of estrus phase in the estrus cycle, bone density and the mammae gland proliferation of rats. The results concluded that MSEE has phytoestrogenic effect on ovariectomized rats.Keywords: phytoestrogen, ovariectomy, uterus weight, bone density, mammae proliferation

ANTIFERTILITY ACTIVITY OF METHANOLIC EXTRACT OF BAUHINIA RACEMOSA LAM BARK IN FEMALE ALBINO RATS

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (09) ◽  
pp. 72-76
Author(s):  
V. Soni ◽  
◽  
A. K. Jha ◽  
J. Dwivedi ◽  
P. Soni
Keyword(s):  
Methanolic Extract ◽  
Ethinyl Estradiol ◽  
Estrogenic Activity ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Luminal Epithelium ◽  
Uterine Weight ◽  
Standard Drug ◽  
Dose Levels

The present study was undertaken with an aim to evaluate the antifertility activity of Bauhinia racemosa bark methanol extract (Br-M extract) by antiovulatory and estrogenic activity models in immature female rats. The extracts were tested for their effect on the estrous cycle at two dose levels of 150 and 300 mg/kg, respectively. Br-M extract at both the dose levels was found to be effective, showing significant antiovulatory activity. For estrogenic activity, the extracts were administered orally once daily at a dose of 150 and 300 mg/kg, and the activity was compared with the standard drug ethinyl estradiol (0.02 mg). The extracts caused significant increase in uterine weight compared to the control. The methanolic extract exhibited estrogenic activity. The histological study of epithelium tissues with the 300 mg of Br-M extract-treated animals showed better increases in the height of the luminal epithelium and loose edematous stroma when compared with the 150 mg of Br-M extract-treated group of animals. However, the lower dose was better than the control group of animals. Enhanced uterine weight and increase in the height of luminal epithelium and histological characteristics suggest that Br-M extract may be useful in anti-fertility therapy.

scholarly journals Folic Acid Affects Iron Status in Female Rats with Deficiency of These Micronutrients

2019 ◽  
Vol 195 (2) ◽  
pp. 551-558 ◽  
Author(s):  
Joanna Suliburska ◽  
Katarzyna Skrypnik ◽  
Agata Chmurzyńska
Keyword(s):  
Folic Acid ◽  
Iron Status ◽  
Iron Concentration ◽  
Folic Acid Supplementation ◽  
Female Rats ◽  
Control Group ◽  
Standard Diet ◽  
Study Group ◽  
Female Wistar Rats ◽  
Iron And Folic Acid

Abstract Although simultaneous supplementation with iron and folic acid is justified, the potential interactions between these micronutrients are unknown. The aim of this study was to determine the effects of oral iron and folic acid, administered together or separately, on iron concentration in tissues in rats with a deficiency of both these micronutrients. In the first stage of the experiment (28 days), 150 8-week-old female Wistar rats were randomly assigned to a control group (C; n = 30) fed the standard diet and to a study group (n = 120) fed a diet deficit in iron and folate. The study group was then randomly divided to four groups: D group fed a deficit diet, FE group fed a deficit diet with iron gluconate, the FOL group fed a deficit diet with folate acid, and the FEFOL group fed a deficit diet with iron gluconate and folate acid. After 2, 10, and 21 days of supplementation, ten animals from each group were killed. Morphological parameters were measured in whole blood. Iron concentration was assayed in serum, liver, spleen, pancreas, heart, and kidneys. Folic acid supplementation more significantly decreased iron concentrations in the pancreas and spleen than in the D group after 10 and 21 days of supplementation. Moreover, the combination of iron with folic acid markedly decreased iron levels in the liver and spleen, in comparison with iron alone, after 10 and 21 days of the experiment. In conclusion, folic acid affects iron status in female rats deficient in these micronutrients in moderate and long-term supplementation.

scholarly journals Pharmacological correction of pathological changes in the viable offspring of rats, caused by cytostatic impact at the stage of progenesis

2019 ◽  
Vol 18 (1) ◽  
pp. 79-85
Author(s):  
T. G. Borovskaya ◽  
M. E. Poluektova ◽  
А. V. Vychuzhanina ◽  
Yu. А. Shchemerovа ◽  
V. E. Goldberg
Keyword(s):  
Female Rats ◽  
Control Group ◽  
Cytostatic Drug ◽  
Pathological Changes ◽  
Group Iii ◽  
Group I ◽  
Child Bearing ◽  
Female Wistar Rats ◽  
Reproductive Aged Women ◽  
Risk Of Cancer

Background. The number of reproductive-aged women with cancer, who desire child bearing, has increased with improvements in cancer detection and treatment. Cancer treatments have the potential to cause germline mutations that might increase the risk of cancer in the progeny of cancer patients. the aim of the study was to assess the feasibility of reducing the long-term side effects of Etoposide on the progeny of rats using Glutaxim. material and methods. Forty-five white outbred female Wistar rats, 2.5-month-old, were divided into 3 groups. Group I consisted of 15 intact rats. Group II comprised 15 rats treated with cytostatic drug (the control group). Group III consisted of 15 rats treated with Glutoxim® (Glutayil-Cysteinyl-Glycine, Pharma Vam Ltd., Russia) at a dose of 50 μg/kg 5 days before and 5 days after receiving cytostatic drug. results. An increase in the number of fetuses with external hemorrhages and pathological changes in internal organs was found in the progeny of female rats receiving Etoposide 3 months before mating. The progeny experienced a decrease in the rate of formation of sensory-motor reflexes, ability to learn and adaptive behavior. All studied parameters did not differ from background values in the progeny of female rats treated with combination of Etoposide and Glutoxim. conclusion. Glutaxim is the effective drug for correction of pathological changes in the progeny of female rats receiving cytostatic drugs.

scholarly journals Dietary Supplementation of Female Rats with Elk Velvet Antler Improves Physical and Neurological Development of Offspring

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Jiongran Chen ◽  
Murray R. Woodbury ◽  
Jane Alcorn ◽  
Ali Honaramooz
Keyword(s):  
Acoustic Startle ◽  
Female Rats ◽  
Neurological Development ◽  
Traditional Use ◽  
Velvet Antler ◽  
Developmental Parameters ◽  
Female Wistar Rats ◽  
Pregnancy And Lactation ◽  
Potential Benefits ◽  
Righting Reflex

Elk velvet antler (EVA) has a traditional use for promotion of general health. However, evidence of EVA effects at different lifestages is generally lacking. This paper investigated the effects of long-term maternal dietary EVA supplementation on physical, reflexological and neurological development of rat offspring. Female Wistar rats were fed standard chow or chow containing 10% EVA for 90 days prior to mating and throughout pregnancy and lactation. In each dietary group, 56 male and 56 female pups were assessed for physical, neuromotor, and reflexologic development postnatally. Among the examined physical developmental parameters, incisor eruption occurred one day earlier in pups nursing dams receiving EVA. Among neuromotor developmental parameters, duration of supported and unsupported standing was longer for pups nursing EVA supplemented dams. Acquisition of neurological reflex parameters (righting reflex, negative geotaxis, cliff avoidance acoustic startle) occurred earlier in pups nursing dams receiving EVA. Longterm maternal EVA supplementation prior to and during pregnancy and lactation accelerated certain physical, reflexologic, and neuromotor developmental milestones and caused no discernible adverse effects on developing offspring. The potential benefits of maternal EVA supplementation on postnatal development warrants further investigation to determine whether EVA can be endorsed for the promotion of maternal and child health.

scholarly journals Direct effect of p,p'- DDT on mice liver

2016 ◽  
Vol 52 (2) ◽  
pp. 287-298 ◽  
Author(s):  
Bárbara Arroyo-Salgado ◽  
Jesús Olivero-Verbel ◽  
Angélica Guerrero-Castilla
Keyword(s):  
Insulin Resistance ◽  
Epidemiological Data ◽  
Vehicle Control ◽  
Sesame Oil ◽  
Pcr Analysis ◽  
Control Group ◽  
Molecular Evidence ◽  
Vehicle Control Group ◽  
Mice Liver

ABSTRACT Contact with the pesticide dichlorodiphenyltrichloroethane (p,p′-DDT) can be the cause of various harmful effects in humans, wildlife, and the environment. This pesticide is known to be persistent, lipophilic, resistant to degradation, and bioaccumulive in the environment and to be slowly released into bloodstream. Growing evidence shows that exposure to DDT is linked to type 2 diabetes mellitus. Individuals exposed to elevated levels of DDT and its metabolite have an increased prevalence of diabetes and insulin resistance. To evaluate these possible relationships, experiments were performed on eight-week-old female mice, divided into three groups (n = 10 per group): Group 1 received a vehicle-control intraperitoneal (i.p.) injection of sesame oil; Groups 2 and 3 received an i.p. dose of 50 and 100 µg/g p,p′-DDT respectively, dissolved in sesame oil. All groups were treated once daily for four days. Real-time PCR analysis of several genes was undertaken. Additionally, biochemical parameters and histopathological changes were measured. NQO1, HMOX1, NR1I3 and NR3C1 were up-regulated in DDT-exposed animals compared to the vehicle control group, while only SREBP1 was down-regulated in the 100 µg/g group. MTTP and FABP5, not previously reported for DDT exposure, but involved in regulation of fatty acid fluxes, could also function as biomarkers cross-talking between these signaling pathways. These results suggest that beyond epidemiological data, there is increasing molecular evidence that DDT may mimic different processes involved in diabetes and insulin resistance pathways.

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