scholarly journals Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples ofHymenocallis littoralisby HPLC-UV Method

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Sreeramanan Subramaniam ◽  
Jeevandran Sundarasekar ◽  
Geethaa Sahgal ◽  
Vikneswaran Murugaiyah
Keyword(s):  
Tissue Culture ◽  
Callus Culture ◽  
Analytical Method ◽  
Reversed Phase ◽  
Raw Material ◽  
Wild Plants ◽  
Wild Plant ◽  
Root Extract ◽  
Minute Amount ◽  
2,4 Dichlorophenoxyacetic Acid

TheHymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue culture samples ofH. littoralis. The separation was achieved using a reversed-phase column. The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the samples. The highest lycorine content was found in the bulb extract (2.54±0.02 μg/mg) whereas the least was in the root extract (0.71±0.02 μg/mg) of the wild plants. Few callus culture samples had high content of lycorine, comparable to that of wild plants. The results showed that plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.5 μM (2.58±0.38 μg/mg) or a combination of 2,4-D at 9.00 μM with 4.5 μM of 6-benzylaminopurine (BAP), were the optimum concentrations for the production of high lycorine (2.45±0.15 μg/mg) content in callus culture. The present analytical method could be of value for routine quantification of lycorine in the tissue culture production and standardization of the raw material or extracts ofH. littoralis.

scholarly journals An Efficient Method for Callus Culture and Shoot Regeneration of Garlic (Allium sativum L.)

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 348-349 ◽  
Author(s):  
Xabier Barandiaran ◽  
Nieves Martín ◽  
María Fernanda Rodríguez-Conde ◽  
Antonio Di Pietro ◽  
Jesus Martín
Keyword(s):  
Tissue Culture ◽  
Callus Culture ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Efficient Method ◽  
Callus Induction ◽  
Allium Sativum ◽  
Regeneration Process ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

The influence of different callus induction media on the regeneration process in garlic was tested. The auxin 2,4 dichlorophenoxyacetic acid frequently used in garlic tissue culture was found to be detrimental when used at the levels described in the literature. However, combinations of growth regulators commonly used for dicot tissue culture produced high levels of callus induction and regeneration that could be used efficiently in a transformation program.

scholarly journals STUDY OF THE ACCUMULATION DYNAMICS AND LOCALIZATION OF 9,13-EPOXYLABDANES IN THE VEGE-TATIVE ORGANS OF INTRODUCED PLANTS OF THE GENUS LAGOCHILUS

2021 ◽  
pp. 247-255
Author(s):  
Fotima Azamzhonovna Sobirova ◽  
Akmal Khushvaktovich Islamov ◽  
Farrukh Nazimovich Tashpulatov ◽  
Umarzhan Nasrutdinovich Zaynutdinov ◽  
Alimzhan Davlatbaevich Matchanov
Keyword(s):  
Extraction Methods ◽  
Raw Material ◽  
Wild Plants ◽  
Wild Plant ◽  
Vegetative Organs ◽  
Introduced Plants ◽  
Urgent Task ◽  
Chemical Studies ◽  
Layer Chromatography ◽  
Derivatives Of

Extracts of the plant Lagochilus inebrians Bunge (Lamiaceae) are used as sedatives, antihypertensive and anti-allergic agents, and to stop bleeding. The main active ingredients of this plant are diterpenoids of the 9-13-epoxylabdanes series, as well as some alkalides. Not rational use of stocks of wild Lagohilus has led to the fact that this plant is currently included in the Red Book. Currently, work is underway to introduce this plant species. Therefore, the study of the dynamics of accumulation and localization of 9,13-epoxylabdans in the vegetative organs of introduced and wild plants of the genus Lagochilus (Lamiaceae) is of interest in terms of collecting the plant for use as a medicinal raw material, for the systematics of data on the accumulation of diterpenoids in the vegetative organs of the plant, for their use. in practical terms, the preparation of diterepenoids and their modification is an urgent task. Some systematic chemical studies of plants of the genus Lagochilus (Lamiaceae) were carried out by the school of Academician A.S. Sadykov. As a result of studying 10 plants of the species Lagochilus (Lamiaceae), more than 20 new diterpenoids of the labdan series were isolated. All of them are natural derivatives of the diterpene alcohol lagochilin. Their structure and configuration were established using IR, PMR and mass spectroscopy, as well as by synthesis from lagochilin or transformation into lagochilin. By studying the composition of diterpenoids of an introduced and wild plant of the genus Lagochilus, it was shown that the main diterpenoids of the 9-13-epoxylabdans series, exhibiting the main biological activity: lagochiline and its acetyl derivatives, accumulate mainly in the leaves and calyx of the plant in August, which is also consistent with some literature data. data. For isolation, extraction methods were used with various solvents. The methods of thin layer chromatography and spectroscopic research methods such as IR, PMR and mass spectrometry were used for identification.

scholarly journals Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3229
Author(s):  
Mat Yunus Najhah ◽  
Hawa Z. E. Jaafar ◽  
Jaafar Juju Nakasha ◽  
Mansor Hakiman
Keyword(s):  
Callus Induction ◽  
Antioxidant Activities ◽  
Shoot Multiplication ◽  
Wild Plants ◽  
Wild Plant ◽  
Total Phenolic ◽  
Nodal Segment ◽  
Ms Medium ◽  
In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

scholarly journals Influence of Cerium Oxide Nanoparticles on Two Terrestrial Wild Plant Species

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 335
Author(s):  
Daniel Lizzi ◽  
Alessandro Mattiello ◽  
Alessio Adamiano ◽  
Guido Fellet ◽  
Emanuele Gava ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Plant Species ◽  
Dry Weight ◽  
Wild Plants ◽  
Wild Plant ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Experimental Conditions ◽  
Wild Plant Species ◽  
Leaf Tissues

Most current studies on the relationships between plans and engineered nanomaterials (ENMs) are focused on food crops, while the effects on spontaneous plants have been neglected so far. However, from an ecological perspective, the ENMs impacts on the wild plants could have dire consequences on food webs and ecosystem services. Therefore, they should not be considered less critical. A pot trial was carried out in greenhouse conditions to evaluate the growth of Holcus lanatus L. (monocot) and Diplotaxis tenuifolia L. DC. (dicot) exposed to cerium oxide nanoparticles (nCeO2). Plants were grown for their entire cycle in a substrate amended with 200 mg kg−1nCeO2 having the size of 25 nm and 50 nm, respectively. nCeO2 were taken up by plant roots and then translocated towards leaf tissues of both species. However, the mean size of nCeO2 found in the roots of the species was different. In D. tenuifolia, there was evidence of more significant particle aggregation compared to H. lanatus. Further, biomass variables (dry weight of plant fractions and leaf area) showed that plant species responded differently to the treatments. In the experimental conditions, there were recorded stimulating effects on plant growth. However, nutritional imbalances for macro and micronutrients were observed, as well.

Development and Validation of a Green Analytical Method for the Determination of Norfloxacin in Raw Material by FTIR Spectrophotometry

2016 ◽  
Vol 99 (6) ◽  
pp. 1533-1536 ◽  
Author(s):  
Jéssica Sayuri Hisano Natori ◽  
Eliane Gandolpho Tótoli ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Urinary Tract ◽  
Organic Solvents ◽  
Analytical Method ◽  
Environmentally Friendly ◽  
Raw Material ◽  
Waste Generation ◽  
Ftir Spectrophotometry ◽  
Tract Infections ◽  
Development And Validation

Abstract Norfloxacin is a broad-spectrum antimicrobial agent, widely used in humans and animals for the treatment of urinary tract infections. It is a second-generation fluoroquinolone. Several analytical methods to analyze norfloxacin have been described in the literature. However, most of them are complex and require the use of large amounts of organic solvents. This paper describes the development and validation of a green analytical method for the determination of norfloxacin in raw material by FTIR spectrophotometry. This method does not require the use of organic solvents, minimizing waste generation in the process and its environmental impacts. The development of methods that promote the reduction, prevention, or elimination of waste generation has become highly attractive to the pharmaceutical industry because of the growing demand from civil society and government authorities for environmentally friendly products and services. The FTIR spectrophotometry method was validated according to International Conference on Harmonization guidelines, showing adequate linearity (r = 0.9936), precision, accuracy, and robustness. This validated method can be used as an environmentally friendly alternative for the quantification of norfloxacin in raw material in QC routine analysis.

scholarly journals Preparation of Graphene/TiO2Composite Nanomaterials and Its Photocatalytic Performance for the Degradation of 2,4-Dichlorophenoxyacetic Acid

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Donggen Huang ◽  
Tianzi Yang ◽  
Zhuanghong Mo ◽  
Qin Guo ◽  
Shuiqing Quan ◽  
...  
Keyword(s):  
High Temperature ◽  
Sol Gel ◽  
Chemical Properties ◽  
Hydrogen Atmosphere ◽  
Raw Material ◽  
Dichlorophenoxyacetic Acid ◽  
Anoxic Water ◽  
Field Environment ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Electrochemical Stripping

The graphene (GR) was prepared by an improved electrochemical stripping method using a high-purity graphite rod as raw material and high temperature heat reduction in hydrogen atmosphere, and the graphene/TiO2(GR/TiO2) composite nanomaterials were manufactured by the method of sol-gel and high temperature crystallization in hydrogen atmosphere using butyl titanate and electrolysis graphene as precursors. The physical and chemical properties of the composites had been characterized by using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), UV-Vis spectrophotometer (UV-Vis), scanning electron microscopy (SEM), Transmission Electron Microscope (TEM),  and specific surface area (SSA) by BET method. The photocatalytic properties of GR/TiO2composites nanomaterials in anoxic water were studied by using 2,4-dichlorophenoxyacetic acid (2,4-D) as probe. The results showed that graphite was well intercalated and peeled by a facile electrolysis method in different electric field environment; a well dispersed and rings structure of graphene was prepared by coupling ultrasound-assisted changing voltage electrochemical stripping technology. The as-prepared GR/TiO2composites had good performance for the photocatalytic degradation of 2,4-D in anoxic water; the chlorines were removed from benzene ring; the middle products of dichlorophenol, chlorophenol, phloroglucinol, and so forth were produced from the photocatalytic redox reaction of 2,4-D in anoxic water; parts of 2,4-D were decomposed completely, and CO2and H2O were produced.

scholarly journals An Identification and Study of Amino Acids of Erigeron annuus Herb

2021 ◽  
Vol 16 (2) ◽  
pp. 88-92
Author(s):  
S. Kovalev ◽  
A. Golovach ◽  
V. Kovalev
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
High Performance ◽  
Total Content ◽  
Amino Acid Profile ◽  
Reversed Phase ◽  
Raw Material ◽  
Precolumn Derivatization ◽  
Amino Acids Composition ◽  
Erigeron Annuus

Amino acids in the extract of Erigeron annuus herb were determined using an automatic precolumn derivatization with fluorenylmethyl-chloroformate and reversed-phase liquid chromatography with fluorescence and UV Vis detection. This objective is reached with automatic derivatization using o-phthalaldehyde (OPA) for primary amino acids and 9-Fluorenylmethyl chloroformate (FMOC) for secondary amino acids. Then derivatization integrates into high performance liquid chromatography (HPLC). The applied procedure is fast with easily reproduced results. The insufficient knowledge about amino acids composition of herb of Erigeron annuus is the basis for study. This work reports on content of 16 free and bound amino acids (391.41 μg/mg) in the plant raw material and influence’s evaluation of different extraction types on the amino acid profile. The total content of free amino acids was 4.66 μg/mg; proline prevailed (2.498 μg/mg). The total content of bound amino acids was 386.7 μg/mg; proline (146.8 μg/mg), arginine (67.8 μg/mg), phenylalanine (25.8 μg/mg), asparagine (24.3 μg/mg), histidine (20.4 μg/mg), alanine (18.2 μg/mg), serine (16.6 μg/mg), valine (16.0 μg/mg) were the dominant amino acids. Nine amino acids were classified as essential.

scholarly journals Diversity of wild edible plants traditionally used by the Galo tribe of Indian Eastern Himalayan state of Arunachal Pradesh

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Padma Raj Gajurel ◽  
Tajum Doni
Keyword(s):  
Relative Frequency ◽  
Vital Role ◽  
Leafy Vegetables ◽  
Wild Edible Plants ◽  
Wild Plants ◽  
Wild Plant ◽  
Edible Plants ◽  
Arunachal Pradesh ◽  
Plant Resources ◽  
Edible Plant

Wild edible plants are found very useful in the fulfilment of food and nutritional requirements. Because of the availibity and cultural preference, the consumption of these plants among the tribes is high. To find out the diversity, utilisation pattern and sociocultural importance of the wild plants, a study was conducted in the state of Arunachal Pradesh selecting the Galo tribe, and accordingly the wild edible plants consumed are documented here. Data were collected through extensive field surveys and interviews with the community in the selected 12 villages in Upper Subansiri and West Siang districts of Arunachal Pradesh. Overall, 125 wild edible plant species under 99 genera and 54 families are reported. These species are consumed mostly as leafy vegetables, fruits, medicine, spices and condiments and as a substitute to food grains. The Urticaceae with ten species is the most utilised family followed by Asteraceae, Moraceae and Lamiaceae with at least five species in each. Herbs with 47 species were found to be the most dominant growth form followed by trees with 44 species. Based on parts used leaves with 66 species were recorded to be the most used plant parts followed by fruits. The highest edibility index of 50 % was reported in Solanum americanum. The analysis of relative frequency of citation revealed that total 78 species exhibits more than 0.50 relative frequency of citation value with highest value in Pouzolzia hirta (0.95). It has been found that the wild plant resources play a vital role in the socio-economic aspects of the Galo tribe.

scholarly journals THE FORMATION OF THE RANGE CONCEPT THE FUNCTIONAL BREAD OF MASS RANGE

2021 ◽  
pp. 64-79
Author(s):  
Irina Pushmina ◽  
Olga Kolman ◽  
Irina Koyupchenko ◽  
Mihail Kudryavtsev
Keyword(s):  
Species Diversity ◽  
Inductively Coupled Plasma ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Combinatorial Analysis ◽  
Wild Plants ◽  
Statistical Research ◽  
Functional Types ◽  
Functional Bread

Determination of the directions of formation of the contemporary level of quality and assortment concept of the species diversity of functional bread of mass assortment with the use of wild berry plants Ericaceae for health improving nutrition. The materials were questionnaires of a survey of potential consumers of functional bread of the city of Krasnoyarsk, wild berry plants of the Heather family (Ericaceae): fruits of cranberry (Vaccinium oxycoccos), of cowberry (Vaccinium vitis-idaea), of bilberry (Vaccinium myrtillus), of blueberry (Vaccinium uliginosum) and powders from them. The method of isolation of the average sample according to GOST 24.027.0-80 was used in the work. The content of toxic elements in berries was determined according to GOST 26927-86, GOST 26930-86, GOST 30178-96 using an atomic emission spectrometer with inductively coupled plasma-iCAP 6500. Generally accepted theoretical and statistical research methods, combinatorial analysis, and data processing using standard mathematical programs were used in the work. Study of the consumer survey, literature data of the chemical composition of berries of wild plants Ericaceae, data on the possibility of procurement of raw materials on the territory of Krasnoyarsk region, investigation of safety performance of the raw material and powders out of him for compliance with the requirements of TR CU 021/2011 and 027/2012 indicate the possibility of their use in the composition of functional bread as a source of biologically active compounds with a variety of positive physiological properties. The multivariance of the development of the range of functional types of bread with additives from Ericaceae berries is confirmed by the data of combinatorial analysis. As a result, an assortment concept of the species diversity of functional bread of a mass assortment of contemporary quality with additives from wild berry plants Ericaceae for improving nutrition was formed. Researches is promising in the formation of the range and actual quality of functional types of bread of mass assortment using local wild-growing raw materials, the advantage of which is naturalness, the content of functional ingredients, and contribute to the development of the range and technologies of healthy food products.

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