Acetylated Hyaluronic Acid: Enhanced Bioavailability and Biological Studies

Hyaluronic acid (HA), a macropolysaccharidic component of the extracellular matrix, is common to most species and it is found in many sites of the human body, including skin and soft tissue. Not only does HA play a variety of roles in physiologic and in pathologic events, but it also has been extensively employed in cosmetic and skin-care products as drug delivery agent or for several biomedical applications. The most important limitations of HA are due to its short half-life and quick degradationin vivoand its consequently poor bioavailability. In the aim to overcome these difficulties, HA is generally subjected to several chemical changes. In this paper we obtained an acetylated form of HA with increased bioavailability with respect to the HA free form. Furthermore, an improved radical scavenging and anti-inflammatory activity has been evidenced, respectively, on ABTS radical cation and murine monocyte/macrophage cell lines (J774.A1).

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Hyaluronic Acid-Based Nanomaterials for Cancer Therapy

Polymers ◽

10.3390/polym10101133 ◽

2018 ◽

Vol 10 (10) ◽

pp. 1133 ◽

Cited By ~ 30

Author(s):

Jin Kim ◽

Myeong Moon ◽

Dong Kim ◽

Suk Heo ◽

Yong Jeong

Keyword(s):

Drug Delivery ◽

Gene Therapy ◽

Extracellular Matrix ◽

Hyaluronic Acid ◽

Combination Therapy ◽

Biomedical Applications ◽

Drug Delivery Systems ◽

Targeted Chemotherapy ◽

Tumor Initiating Cells ◽

Good Potential

Hyaluronic acid (HA) is a nonsulfated glycosaminoglycan and a major component of the extracellular matrix. HA is overexpressed by numerous tumor cells, especially tumor-initiating cells. HA-based nanomaterials play in importance role in drug delivery systems. HA is used in various types of nanomaterials including micelle, polymersome, hydrogel, and inorganic nanoparticle formulations. Many experiments show that HA-based nanomaterials can serve as a platform for targeted chemotherapy, gene therapy, immunotherapy, and combination therapy with good potential for future biomedical applications in cancer treatment.

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Cellular responses of hyaluronic acid-coated chitosan nanoparticles

Toxicology Research ◽

10.1039/c8tx00041g ◽

2018 ◽

Vol 7 (5) ◽

pp. 942-950 ◽

Cited By ~ 5

Author(s):

Abdulaziz Almalik ◽

Ibrahim Alradwan ◽

Majed A. Majrashi ◽

Bashayer A. Alsaffar ◽

Abdulmalek T. Algarni ◽

...

Keyword(s):

Drug Delivery ◽

Hyaluronic Acid ◽

Biomedical Applications ◽

Chitosan Nanoparticles ◽

Cellular Responses

In recent years, nanotechnology has been proven to offer promising biomedical applications for in vivo diagnostics and drug delivery, stressing the importance of thoroughly investigating the biocompatibility of potentially translatable nanoparticles (NPs).

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Understanding Microdroplet Formations for Biomedical Applications

Volume 15: Processing and Engineering Applications of Novel Materials ◽

10.1115/imece2008-69223 ◽

2008 ◽

Author(s):

Salil Desai ◽

Anthony Moore ◽

Benjamin Harrison ◽

Jagannathan Sankar

Keyword(s):

Drug Delivery ◽

Sodium Chloride ◽

Sodium Alginate ◽

Calcium Chloride ◽

Biomedical Applications ◽

Tissue Scaffolds ◽

Ambient Conditions ◽

On Demand ◽

Drop On Demand

This paper focuses on understanding microdroplet formation of sodium alginate biopolymer at various concentrations utilizing drop-on-demand inkjet technology. We investigate the effect of sodium chloride on the rheology of sodium alginate and derive a correlation between the size of the droplet versus the size of the microcapsules formed. Varying sizes of microcapsules are formed based on different concentrations of calcium chloride solvent. This understanding will give insight for fabricating drug delivery capsules and tissue scaffolds that are subject to extreme ambient conditions when interfaced with in-vivo environments.

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A Review on Application of Novel Solid Nanostructures in Drug Delivery

Journal of Nano Research ◽

10.4028/www.scientific.net/jnanor.53.22 ◽

2018 ◽

Vol 53 ◽

pp. 22-36 ◽

Cited By ~ 4

Author(s):

Habibollah Faraji ◽

Reza Nedaeinia ◽

Esmaeil Nourmohammadi ◽

Bizan Malaekeh-Nikouei ◽

Hamid Reza Sadeghnia ◽

...

Keyword(s):

Drug Delivery ◽

Drug Therapy ◽

Biomedical Applications ◽

Imaging Biomarkers ◽

Cellular Functions ◽

Scientific Innovation ◽

Wide Range ◽

Targeted Drug

Nanotechnology as a multidisciplinary and scientific innovation plays an important role in numerous biomedical applications, such as molecular imaging, biomarkers and biosensors and also drug delivery. A wide range of studies have been conducted on using of nanoparticles for early diagnosis and targeted drug therapy of various diseases. In fact, the small size, customized surface, upgraded solubility, or multi-functionality of nanoparticles enabled them to interact with complex cellular functions in new ways which opened many doors and created new biomedical applications. These studies demonstrated that nanotechnology vehicles can formulate biological products effectively, and this nano-formulated products with a potent ability against different diseases, were represented to have better biocompatibility, bioaccessibility and efficacy, under in vitro and in vivo conditions.

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Stability of Engineered Micro or Nanobubbles for Biomedical Applications

Pharmaceutics ◽

10.3390/pharmaceutics12111089 ◽

2020 ◽

Vol 12 (11) ◽

pp. 1089

Author(s):

Beomjin Park ◽

Semi Yoon ◽

Yonghyun Choi ◽

Jaehee Jang ◽

Soomin Park ◽

...

Keyword(s):

Drug Delivery ◽

Biomedical Applications ◽

Ultrasonic Field ◽

Nanometer Scale ◽

Factors Affecting ◽

The Core ◽

Bubble Structure ◽

The Stability ◽

The Relationship

A micro/nanobubble (MNB) refers to a bubble structure sized in a micrometer or nanometer scale, in which the core is separated from the external environment and is normally made of gas. Recently, it has been confirmed that MNBs can be widely used in angiography, drug delivery, and treatment. Thus, MNBs are attracting attention as they are capable of constructing a new contrast agent or drug delivery system. Additionally, in order to effectively use an MNB, the method of securing its stability is also being studied. This review highlights the factors affecting the stability of an MNB and the stability of the MNB within the ultrasonic field. It also discusses the relationship between the stability of the bubble and its applicability in vivo.

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Natural Polysaccharides for siRNA Delivery: Nanocarriers Based on Chitosan, Hyaluronic Acid, and Their Derivatives

Molecules ◽

10.3390/molecules24142570 ◽

2019 ◽

Vol 24 (14) ◽

pp. 2570 ◽

Cited By ~ 14

Author(s):

Inés Serrano-Sevilla ◽

Álvaro Artiga ◽

Scott G. Mitchell ◽

Laura De Matteis ◽

Jesús M. de la Fuente

Keyword(s):

Drug Delivery ◽

Hyaluronic Acid ◽

Small Interfering Rna ◽

Drug Delivery Systems ◽

Sirna Delivery ◽

Delivery Systems ◽

Low Toxicity ◽

Interfering Rna

Natural polysaccharides are frequently used in the design of drug delivery systems due to their biocompatibility, biodegradability, and low toxicity. Moreover, they are diverse in structure, size, and charge, and their chemical functional groups can be easily modified to match the needs of the final application and mode of administration. This review focuses on polysaccharidic nanocarriers based on chitosan and hyaluronic acid for small interfering RNA (siRNA) delivery, which are highly positively and negatively charged, respectively. The key properties, strengths, and drawbacks of each polysaccharide are discussed. In addition, their use as efficient nanodelivery systems for gene silencing applications is put into context using the most recent examples from the literature. The latest advances in this field illustrate effectively how chitosan and hyaluronic acid can be modified or associated with other molecules in order to overcome their limitations to produce optimized siRNA delivery systems with promising in vitro and in vivo results.

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Cumulus Cell Expansion, Its Role in Oocyte Biology and Perspectives of Measurement: A Review

Scientia Agriculturae Bohemica ◽

10.1515/sab-2015-0002 ◽

2015 ◽

Vol 45 (4) ◽

pp. 212-225 ◽

Cited By ~ 4

Author(s):

J. Nevoral ◽

M. Orsák ◽

P. Klein ◽

J. Petr ◽

M. Dvořáková ◽

...

Keyword(s):

Extracellular Matrix ◽

Hyaluronic Acid ◽

High Performance ◽

Uv Light ◽

Cumulus Cell ◽

Developmental Competence ◽

Enzyme Linked Immunosorbent Assay ◽

Cumulus Expansion

Abstract Cumulus expansion of the cumulus-oocyte complex is necessary for meiotic maturation and acquiring developmental competence. Cumulus expansion is based on extracellular matrix synthesis by cumulus cells. Hyaluronic acid is the most abundant component of this extracellular matrix. Cumulus expansion takes place during meiotic oocyte maturation under in vivo and in vitro conditions. Quantification and measurement of cumulus expansion intensity is one possible method of determining oocyte quality and optimizing conditions for in vitro cultivation. Currently, subjective methods of expanded area and more exact cumulus expansion measurement by hyaluronic acid assessment are available. Among the methods of hyaluronic acid measurement is the use of radioactively labelled synthesis precursors. Alternatively, immunological and analytical methods, including enzyme-linked immunosorbent assay (ELISA), spectrophotometry, and high-performance liquid chromatography (HPLC) in UV light, could be utilized. The high sensitivity of these methods could provide a precise analysis of cumulus expansion without the use of radioisotopes. Therefore, the aim of this review is to summarize and compare available approaches of cumulus expansion measurement, respecting special biological features of expanded cumuli, and to suggest possible solutions for exact cumulus expansion analysis.

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Smart pH-responsive and high doxorubicin loading nanodiamond for in vivo selective targeting, imaging, and enhancement of anticancer therapy

Journal of Materials Chemistry B ◽

10.1039/c6tb00266h ◽

2016 ◽

Vol 4 (29) ◽

pp. 5046-5058 ◽

Cited By ~ 15

Author(s):

Lin Li ◽

Lu Tian ◽

Yongli Wang ◽

Wenjing Zhao ◽

Fangqin Cheng ◽

...

Keyword(s):

Drug Delivery ◽

Biomedical Applications ◽

Anticancer Therapy ◽

Effective Strategy ◽

Ph Responsive ◽

Selective Targeting ◽

Delivery Platform

We introduce a simple and effective strategy to design a promising drug delivery platform for improving the biomedical applications of smart nanodiamond carriers.

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Hyaluronic acid-functionalized gelatin hydrogels reveal extracellular matrix signals temper the efficacy of erlotinib against patient-derived glioblastoma specimens

10.1101/556324 ◽

2019 ◽

Author(s):

Sara Pedron ◽

Gabrielle L. Wolter ◽

Jee-Wei E. Chen ◽

Sarah E. Laken ◽

Jann N. Sarkaria ◽

...

Keyword(s):

Extracellular Matrix ◽

Hyaluronic Acid ◽

Tumor Microenvironment ◽

Kinase Inhibitor ◽

Egfr Mutations ◽

Primary Glioblastoma ◽

Stat3 Phosphorylation

AbstractTherapeutic options to treat primary glioblastoma (GBM) tumors are scarce. GBM tumors with epidermal growth factor receptor (EGFR) mutations, in particular a constitutively active EGFRvIII mutant, have extremely poor clinical outcomes. GBM tumors with concurrent EGFR amplification and active phosphatase and tensin homolog (PTEN) are sensitive to the tyrosine kinase inhibitor erlotinib, but the effect is not durable. A persistent challenge to improved treatment is the poorly understood role of cellular, metabolic, and biophysical signals from the GBM tumor microenvironment on therapeutic efficacy and acquired resistance. The intractable nature of studying GBM cell in vivo motivates tissue engineering approaches to replicate aspects of the complex GBM tumor microenvironment. Here, we profile the effect of erlotinib on two patient-derived GBM specimens: EGFR+ GBM12 and EGFRvIII GBM6. We use a three-dimensional gelatin hydrogel to present brain-mimetic hyaluronic acid (HA) and evaluate the coordinated influence of extracellular matrix signals and EGFR mutation status on GBM cell migration, survival and proliferation, as well as signaling pathway activation in response to cyclic erlotinib exposure. Comparable to results observed in vivo for xenograft tumors, erlotinib exposure is not cytotoxic for GBM6 EGFRvIII specimens. We also identify a role of extracellular HA (via CD44) in altering the effect of erlotinib in GBM EGFR+ cells by modifying STAT3 phosphorylation status. Taken together, we report an in vitro tissue engineered platform to monitor signaling associated with poor response to targeted inhibitors in GBM.

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Monitoring the Effect of Transdermal Drug Delivery Patches on the Skin Using Terahertz Sensing

Pharmaceutics ◽

10.3390/pharmaceutics13122052 ◽

2021 ◽

Vol 13 (12) ◽

pp. 2052

Author(s):

Hannah Lindley-Hatcher ◽

Jiarui Wang ◽

Arturo I. Hernandez-Serrano ◽

Joseph Hardwicke ◽

Gabit Nurumbetov ◽

...

Keyword(s):

Drug Delivery ◽

Water Content ◽

Biomedical Applications ◽

Skin Barrier ◽

High Sensitivity ◽

Thz Spectroscopy ◽

Transdermal Patches ◽

Patch Removal ◽

Terahertz Sensing

Water content of the skin is an important parameter for controlling the penetration rate of chemicals through the skin barrier; therefore, for transdermal patches designed for drug delivery to be successful, the effects of the patches on the water content of the skin must be understood. Terahertz (THz) spectroscopy is a technique which is being increasingly investigated for biomedical applications due to its high sensitivity to water content and non-ionizing nature. In this study, we used THz measurements of the skin (in vivo) to observe the effect of partially and fully occlusive skin patches on the THz response of the skin after the patches had been applied for 24 h. We were able to observe an increase in the water content of the skin following the application of the patches and to identify that the skin remained hyper-hydrated for four hours after the removal of the fully occlusive patches. Herein, we show that THz spectroscopy has potential for increasing the understanding of how transdermal patches affect the skin, how long the skin takes to recover following patch removal, and what implications these factors might have for how transdermal drug patches are designed and used.

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