scholarly journals Influence of NAA and coconut water with variation of soaking duration on the growth of yellow bamboo branch cutting

2018 ◽  
Vol 10 (3) ◽  
pp. 178-182
Author(s):  
TIA SETIAWATI ◽  
AGINTA PUTRI REHULINA KELIAT ◽  
RULY BUDIONO ◽  
RUHYAT PARTASASMITA ◽  
JOHAN ISKANDAR
Keyword(s):  
Leaf Area ◽  
Root Length ◽  
Raw Materials ◽  
Shoot Length ◽  
Coconut Water ◽  
Root Number ◽  
Randomized Design ◽  
Shoot Number ◽  
Bambusa Vulgaris ◽  
Branch Cutting

Setiawati T, Keliat APR, Budiono R, Partasasmita R, Iskandar J. 2018. Influence of NAA and coconut water with variation of soaking duration on the growth of yellow bamboo branch cutting. Nusantara Bioscience 10: 178-182. Yellow bamboo (Bambusa vulgaris Schrad Ex. var. Striata) is one kind of bamboo used for industrial and household raw materials. Yellow bamboo propagation with branch cuttings has relatively low percentage of growth. To increase the growing success of yellow bamboo branch cuttings can be used growth regulators substance, such as NAA and coconut water. This study aimed to obtain the best combination of NAA and coconut water with the soaking duration in the growth of yellow bamboo branch cuttings. The study was conducted using Completely Randomized Design (CRD) of 6 × 3 factorial arrangement with five replications. The first factor was the combination of NAA and coconut water (CW), which consisted of six levels, namely 100% CW, 20% NAA + 80% CW, 40% NAA + 60% CW, 60% NAA + 40% CW, 80% NAA + 20% CW and 100% NAA. The second factor was the soaking duration which consists of three levels, namely 12 h, 24 h, and 36 h. Parameters observed included shoot emerging time, shoot number, shoot length, leaf area, root number and root length. The data obtained were analyzed using the Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) α=5%. The results showed that the combination of 80% NAA + 20% CW gave the best yellow bamboo branch cuttings growth with an average shoot length of 1.44 cm, leaf area 41.29 cm2, root number 42.87 and root length 23.70 cm. The interaction of 80% NAA + 20% CW with soaking duration of 36 h resulted in average the fastest shoot emerging time of 2.02 days after planting.

scholarly journals PEMANFAATAN AIR KELAPA UNTUK MENINGKATKAN PERTUMBUHAN AKAR STEK TUNAS AKSILAR Andrographis paniculata Nees

2017 ◽  
Vol 18 (02) ◽  
pp. 62-68
Author(s):  
Retno Prihatini
Keyword(s):  
Root Length ◽  
Water Concentration ◽  
Height Growth ◽  
Andrographis Paniculata ◽  
Coconut Water ◽  
Axillary Buds ◽  
Root Number ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Cutting Height

The research has been conducted to find out the best concentration of coconut water in the induction of roots of cuttings axillary buds Andrographis paniculata Nees. This research was conducted in Plant Physilogy of Division,   Department of Biology, Faculty of Math and Natural Science, AndalasUniversity, for 12 weeks.  The research used Completely Randomized Design. The material used was axillary buds  A.  paniculata  with nodal number 3. treated for 30 minutes in coconut water 10, 15 and 20% and aquadest as a controle each with 6 replications. Observations were done on live percentage, root length, root  numerous and  cuttings  height  after 12 weeks planting. The results showed that the  concentration 10 to 15% of coconut water was able with significant  to induce the  root growth of cutting axillary buds A. Paniculat.  The concentration 15% of coconut water was the best in inducing  cutting height growth, each with an average root length is 8.2 cm and the average number of roots that formed is 16, 0 and the average cutting height is  22.7 cm. There was an increase of average of  cuttings height growth by 86% which was supported by the average increase of root length and root number of 105.0% and 280.9% on the occasion of cuttings of axillary buds in coconut water concentration 15%.

scholarly journals Respon Setek Cabang Bambu Kuning (Bambusa Vulgaris) Terhadap Pemberian Aia

2014 ◽  
Vol 2 (1) ◽  
pp. 95
Author(s):  
Yosepin K. Simangunsong ◽  
Indriyanto . ◽  
Afif Bintoro
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Shoot Length ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Bambusa Vulgaris ◽  
Bartlett Test ◽  
Number Of Leaves ◽  
Branch Cutting

Yellow bamboo is one of the bamboo species which used for industrial and house-hold materials, because it has a thick trunk outer layer, length of fiber, and beautiful shape.The success of cuttings grown yellow bamboo branch was lack.To increase the success of the yellow bamboo branches grow cuttings used growth regulators.Therefore, the research was conducted by giving indole acetic acid on yellow bamboo branch cuttings.The objective of this research was to known the growth of yellow bamboo branch cuttings by giving indole acetic acid and known the concentration of the indole acetic acid were the best influence on the growth of yellow bamboo branch cuttings.The research was designed with complete randomized design (CRD) with five treatment,five experimental units, and five branch cuttings for each experimental unit.The concentration ofindole acetic acid (IAA) was 0 ppm, 100 ppm, 200 ppm,300 ppm, and 400 ppm.To test the homogeneity range done with Bartlett test, the data will be tested by analysis of variance and then further tested with honestly significant difference test.Yellow bamboo branch cuttings growth by percentage of life parameters, number of shoots, shoot length, shoot diameter, and a significant number of leaves is known, whereas for root length parameter is not significant. Keywords: branch cutting, indole acetic acid, yellow bamboo

scholarly journals Modification of Media for Banana In Vitro Propagation with Foliar Fertilizer and Coconut Water in cv. Rajabulu

2019 ◽  
Vol 35 (1) ◽  
pp. 23
Author(s):  
Siti Mardhikasari ◽  
Ahmad Yunus ◽  
Samanhudi Samanhudi
Keyword(s):  
Root Length ◽  
Leaf Length ◽  
Shoot Length ◽  
Coconut Water ◽  
Root Number ◽  
Foliar Fertilizer ◽  
Two Factors ◽  
The Media ◽  
Four Levels

The use of foliar fertilizers and coconut water is potential; foliar fertilizer is the alternative medium and coconut water is the plant growth regulator (PGR) in the banana tissue culture. This study aimed to examine the ability of foliar fertilizer to substitute Murashige and Skoog (MS) media and coconut water as the main source of cytokine. There are two factors in this research. The first factor is the media, which consist of three levels, i.e. fully MS, ½ MS + ½ foliar fertilizer and fully foliar fertilizer. The second factor is the coconut water with four levels of concentration i.e. 50, 100, 150 and 200 ml l<sup>-1</sup>. The results show that fully formulated foliar fertilizer had not been able to substitute or even compete with a fully MS media, but conversely foliar fertilizer was used to substitute ½ MS media. This can be seen from the parameters of shoot length, leaf length, root number and root length with the highest yield that was found in MS media (8.7, 4.66, 3.33 and 3.23 cm). The 50-100 ml l<sup>-1 </sup>concentrations of coconut water showed the best results by giving a significant effect on the number of roots and root length. The acclimatization of plantlets in this study was nearly 100%, indicated by the formation of a complete organ.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatic Embryogenesis (The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses).

2012 ◽  
Vol 28 (2) ◽  
pp. 82-90
Author(s):  
Rina Arimarsetiowati ◽  
Fitria Ardiyani
Keyword(s):  
Leaf Area ◽  
Root Length ◽  
Combined Treatment ◽  
Stem Diameter ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Half Strength ◽  
The Media ◽  
Number Of Leaves ◽  
High Level

Plantlet that has developed shoots and roots will have a high level adaptation in the field. The objective of this experiment was to improve the ability of planlet in shooting and rooting so that it is ready for acclimatization in the field. The increase ability in shooting and rooting of the planlet were conducted by adding various types of auxin in the media. The arabica coffee embryo of clone AS 2K which has entered the phase of the cotyledons was transfered into the treatment media containing half-strength of MS (Murashige & Skoog) macro and micro nutrient, vitamin B5, 30 g/L glucose, 100 ml/L coconut water, 50 mg/L AgNO3 added with the combination of IAA, IBA and NAA. The research was conducted by using completely randomized design with seven combined treatment i.e. 0.1 mg/L IBA, 0.1 mg/L NAA, 0.1 mg/L IAA; 0 , 1 mg/L IBA + 0.1 mg/L NAA, 0.1 mg/L IBA + 0.1 mg/L IAA, 0.1 mg/L NAA + 0.1 mg/L IAA; without auxin. There were 12 replications in every treatment and each replication consisted of five cotyledonary embryos. The parameters of observation were the root length, leaf number, leaf area, stem diameter, and height of plantlets. The observations were conducted in eighth weeks after cotyledonary embryo had shoots. The results showed that in the number of leaves and height of planlet parameters, the treatment without auxin was the best result compared to planlet with auxin addition. The addition of auxin varians and their combination did not significantly influent leaf area, root length and stem diameter parameters. The medium tested was optimum for the growth of shoots and roots of AS 2K arabica coffee.

scholarly journals Influence of Organic Supplements on Growth and Development of In Vitro Shoots of Bulbophyllum dhaninivatii Seidenf

2016 ◽  
Vol 855 ◽  
pp. 42-46 ◽  
Author(s):  
Anupan Kongbangkerd ◽  
Santi Watthana ◽  
Kanok Orn Srimuang
Keyword(s):  
Growth And Development ◽  
Shoot Culture ◽  
Coconut Water ◽  
Root Number ◽  
Shoot Number ◽  
Potato Extract ◽  
Semi Solid

In Vitro shoot culture of Bulbophyllum dhaninivatii Seidenf. was conducted on semi-solid Vacin and Went (1949) medium supplemented with coconut water (50 100 and 150 ml/L), potato extract (25 and 50 g/L) and banana homogenate (25 and 50 g/L) for 12 weeks. The results found that the highest shoot number (6.92 shoots) could observe on the medium supplemented with 150 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate while the highest leaf and root number could obtain when cultured on the medium supplemented with 100 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate.

scholarly journals Effects of lead on seedling growth of spesia populnea

2010 ◽  
Vol 56 (No. 4) ◽  
pp. 194-199 ◽  
Author(s):  
M. Kabir ◽  
M.Z. Iqbal ◽  
M. Shafiq ◽  
Z.R. Farooqi
Keyword(s):  
Leaf Area ◽  
Environmental Conditions ◽  
Root Length ◽  
Growth And Development ◽  
Strong Influence ◽  
Dry Weight ◽  
Shoot Length ◽  
Thespesia Populnea ◽  
Seedling Length ◽  
Number Of Leaves

The effects of lead on root, shoot and seedling length, leaf area, number of leaves, plant circumference, seedling dry weight, root/shoot and leaf area ratios of <I>Thespesia populnea</I> L. were determined in greenhouse under natural environmental conditions with and without phytotoxic metal ions at 5, 10, 15, 20, and 25 µmol/l. Lead treatments have a strong influence on the growth and development of <I>T</I>. <I>populnea</I> by reducing significantly (<I>P</I> &lt; 0.05) all the above parameters. Lead treatment at 5–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on seedling and root length, plant circumference and seedling dry weight of <I>T</I>.<I> populnea,</I> while lead treatment at 10–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on shoot length, number of leaves and leaf area as compared to control. Tolerance in <I>T</I>.<I> populnea</I> seedling at 25 µmol/l of lead treatment was lowest as compared to all other treatments.

scholarly journals Dynamics of aerial and perennial biomass of two lucerne cultivars with different fall dormancy subject to two severities of cutting during the establishment phase

2021 ◽  
Vol 120 (1) ◽  
pp. 072
Author(s):  
Leonela Analía García ◽  
Silvia Graciela Assuero ◽  
Gabriel Blain ◽  
Matías Ezequiel Pereyra ◽  
Germán Darío Berone
Keyword(s):  
Leaf Area ◽  
The Other ◽  
Randomized Design ◽  
Shoot Number ◽  
Fall Dormancy ◽  
Crown Biomass ◽  
Medicago Sativa L ◽  
Completely Randomized Design ◽  
The Impact ◽  
Establishment Phase

The aim of this study was to quantify the impact of two cutting severities on the dynamics of aerial and perennial (root plus crown) biomass of lucerne (Medicago sativa L.) plants of two cultivars of contrasting fall dormancy rating during the establishment phase. We worked at Unidad Integrada Balcarce with two cultivars of lucerne (non-winter dormant: FD10; semi-winter dormant: FD6) and two cutting severities (40 and 100 mm) defining four treatments (FD6-40 mm, FD6-100 mm, FD10-40 mm and FD10-100 mm) under a completely randomized design. In both cultivars, increasing cutting severity produced a reduction in leaf area and number of nodes in the residual, resulting in a reduced production of aerial biomass during regrowth, given a reduction on shoot number. On the other hand, the effect of increased cutting severity on perennial biomass was only observed in plants of FD10 cultivar. This cultivar showed less perennial biomass when cut at 40 mm than at 100 mm, while no differences were observed between cutting heights in FD6 cultivar.

scholarly journals Response of ‘Bignay’ [Antidesma bunius (Linn.) Spreng] to cutting origins, IBA and BioGroe treatments

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Edgar V Benabise ◽  
Jonalyn J Quinan ◽  
Joel G Carig
Keyword(s):  
Butyric Acid ◽  
Interaction Effect ◽  
Shoot Length ◽  
Root Number ◽  
Percent Survival ◽  
Randomized Design ◽  
Study Results ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Bottom Portion

The response of ‘Bignay’ [Antidesma bunius (Linn.) Spreng] to the cutting origins and different levels of plant bio-regulators consist of Indole-3-butyric Acid (IBA) and Biogroe treatments were investigated by means of 3 x 9 factorial experiment in Completely Randomized Design (CRD) using an automated mist propagator. Two hundred sixteen (216) healthy seedlings containing 9 nodes each were used in the study. Results revealed that cutting origins significantly increased shoot length but have no influence on the root number, percent rooting and percent survival. The cuttings originated from the bottom portion of the stem recorded the longest mean in terms of shoot length (12.48 mm) including the highest percent survival and percent rooting (82.41%). Highest mean number of roots were observed on the top cuttings (1.93). Indole-3-butyric Acid (IBA) and Biogroe treatments on cuttings have no effects on the different parameters evaluated. The interaction effect between cutting origins and IBA/Biogroe treatments significantly increased the percent rooting and percent survival except the shoot length and root number of Bignay cuttings. Overall, the findings inferred that A. bunius can be propagated by any cutting origin derived from the main stem of the donor plants tested. Cuttings can effectively be induced to produce roots and survive and can be economically mass propagated even without the application of different concentrations of IBA and BioGroe.

scholarly journals Pengaruh Aplikasi Biostimulant terhadap Pertumbuhan dan Produksi Selada (Lactuca sativa L.)

2020 ◽  
Vol 16 (2) ◽  
pp. 109-117
Author(s):  
Primarine R Tahapary ◽  
Herman Rehatta ◽  
Henry Kesaulya
Keyword(s):  
Vitamin E ◽  
Plant Growth ◽  
Vitamin C ◽  
Leaf Area ◽  
Root Length ◽  
Block Design ◽  
Growth And Yield ◽  
Leaf Number ◽  
Root Number ◽  
Fresh Weight

Lettuce is a type of vegetable that is very popular with the people of Indonesia. It contains healthy nutrients including minerals, vitamins, antioxidants, potassium, iron, folate, carotene, vitamin C and vitamin E. Biostimulants has the ability to stimulate new root formation, improve plant root systems, increase the number of tillers, and increase yield. Biostimulant concentration and site of administration play a role in providing nutrients for the needs of lettuce plants. To obtain the right concentration and place of administration for the growth and yield of lettuce, the experiment was carried out using a 2-factor Randomized Block Design. The first factor was the concentration of biostimulants (K) with 6 levels of concentration including: K0 (without giving biostimulants), K1 (giving biostimulants at a concentration of 1 mL/L of water), K2 (giving of biostimulants at 2 mL/L of water), K3 (giving biostimulants at 3 mL/L of water). K4 (giving biostimulant at 4 mL/L water), K5 (giving biostimulant at 5 mL/L water). The second factor was the site of administration (T), namely: T1 (through the leaves) and T2 (through the soil). The interaction between biostimulant concentration and administration gave a very significant effect on leaf number, crop fresh weight, root length and root number. Biostimulant concentration (K) gave the percentage of plant growth in the variables of plant height (34.29%) and leaf area (47.34%). Place of administration (T) gave percentage of plant growth the variables of leaf area (21.08%) and root wet weight (0.52%). The interaction of biostimulant concentration and administration place (K x T) gave the percentages of plant growth variables of leaf number (15.5%), plant fresh weight (52.33%), root dry weight (2.30%), root length (16.53%), and root number (108.5%). The best treatment in this study was the provision of biostimulant at a concentration of 2 mL/L of water that was given through leaves. Keywords: administration place, biostimulant, concentration, growth and production, lettuce   ABSTRAK Selada merupakan jenis sayur yang sangat digemari oleh masyarakat Indonesia serta mengandung nutrisi yang sehat, yang meliputi mineral, vitamin, antioksidan, potassium, zat besi, folat, karoten, vitamin C dan vitamin E. Biostimulan mampu merangsang pembentukan akar baru, memperbaiki sistem perakaran tanaman, memperbanyak jumlah anakan, dan meningkatkan produksi tanaman. Konsentrasi biostimulan dan tempat pemberian merupakan interaksi yang berperan dalam menyediakan unsur hara bagi kebutuhan tanaman selada. Untuk memproleh konsentrasi dan tempat pemberian yang tepat bagi pertumbuhan dan produksi tanaman selada, dilakukan percobaan dengan menggunakan Rancangan Acak Kelompok 2 faktor. Faktor pertama adalah pemberian biostimulan (K) dengan 6 taraf konsentrasi meliputi: K0 (tanpa pemberian biostimulan), K1 (pemberian biostimulan dengan konsentrasi 1 mL/L air), K2 (pemberian biostimulan 2 mL/L air), K3 (pemberian biostimulan 3 mL/L air), K4 (pemberian biostimulan 4 mL/L air), K5 (pemberian biostimulan 5 mL/L air). Faktor kedua adalah tempat pemberian (T), yaitu: T1 (melalui daun) dan T2 (melalui tanah). Interaksi konsentrasi biostimulan berpengaruh sangat nyata terhadap variabel jumlah daun (helai), bobot segar tanaman (g), panjang akar (cm) dan jumlah akar (helai). Pada konsentrasi biostimulan (K) memberikan persentasepertumbuhan tanaman pada variabel tinggi tanaman (34,29%) dan luas daun (47,34%), Tempat pemberian (T) memberikan persentasepertumbuhan tanaman pada variabel luas daun (21,08%), bobot basah akar (0,52%), serta interaksi konsentrasi biostimulan dan tempat pemberian (KT) memberikan persentasepertumbuhan tanaman pada variabel jumlah daun (15,5%), bobot segar tanaman (52,33%), bobot kering akar (2,30%), panjang akar (16,53%), serta jumlah akar (108,5%). Perlakuan terbaik dalam penelitian ini adalah pemberian biostimulan konsentrasi 2 mL/L air dan diberikan melalui daun. Kata Kunci: biostimulan, konsentrasi, pertumbuhan dan produksi, selada, tempat pemberian,

Sign in / Sign up

Export Citation Format

Share Document