scholarly journals Micro- and Nanosized Particles in Nasal Mucosa: A Pilot Study

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Lenka Čábalová ◽  
Kristina Čabanová ◽  
Hana Bielniková ◽  
Jana Kukutschová ◽  
Jana Dvořáčková ◽  
...  
Keyword(s):  
Elemental Composition ◽  
Nasal Mucosa ◽  
Solid Particles ◽  
Nanosized Particles ◽  
Tissue Samples ◽  
Employment History ◽  
Interindividual Differences ◽  
Barium Copper ◽  
Novel Method ◽  
Scanning Electron

Objective. The aim of this prospective study is to evaluate presence and quantity of micro- and nanosized particles (NPs) and interindividual differences in their distribution and composition in nasal mucosa.Methods. Six samples of nasal mucosa obtained by mucotomy from patients with chronic hypertrophic rhinosinusitis were examined. Samples divided into 4 parts according to the distance from the nostrils were analyzed by scanning electron microscopy and Raman microspectroscopy to detect solid particles and characterize their morphology and composition. A novel method of quantification of the particles was designed and used to evaluate interindividual differences in distribution of the particles. The findings were compared with patients’ employment history.Results. In all the samples, NPs of different elemental composition were found (iron, barium, copper, titanium, etc.), predominantly in the parts most distant from nostrils, in various depths from the surface of the mucosa and interindividual differences in their quantity and composition were found, possibly in relation to professional exposition.Conclusions. This study has proven the possibility of quantification of distribution of micro- and nanosized particles in tissue samples and that the NPs may deposit in deeper layers of mucosa and their elemental composition may be related to professional exposition to the sources of NPs.

scholarly journals Histologic Aspect of the Curved Vomerine Mucosa in Cleft Lip and Palate

2021 ◽  
pp. 105566562110314
Author(s):  
Benito K. Benitez ◽  
Andrzej Brudnicki ◽  
Prasad Nalabothu ◽  
Jeannette A. von Jackowski ◽  
Elisabeth Bruder ◽  
...  
Keyword(s):  
Nasal Mucosa ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Squamous Metaplasia ◽  
Periodic Acid ◽  
Surgical Techniques ◽  
Basal Cells ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Nasal Floor

Background: Common surgical techniques aim to turn the entire vomerine mucosa with vomer flaps either to the oral side or to the nasal side. The latter approach is widely performed due to the similarity in color to the nasal mucosa. However, we lack a histologic description of the curved vomerine mucosa in cleft lip and palate malformations. Methods: We histologically examined an excess of curved vomerine mucosa in 8 patients using hematoxylin–eosin, periodic acid–Schiff, Elastin van Gieson, and Alcian blue stains. Tissue samples were obtained during surgery at 8 months of age. Results: Our histological analysis of the mucoperiosteum overlying the curved vomer revealed characteristics consistent with those of an oral mucosa or a squamous metaplasia of the nasal mucosa, as exhibited by a stratified squamous epithelium containing numerous seromucous glands. Some areas showed a palisaded arrangement of the basal cells compatible with metaplasia of respiratory epithelium, but no goblet cells or respiratory cilia were identified. Abundant fibrosis and rich vascularity were present. Conclusion: The vomer mucosa showed no specific signs of nasal mucosa. These findings should be considered in presurgical cleft orthopedics and palatal surgery for further refinement. Shifting the vomer mucosa according to a fixed physiologic belief should not overrule other important aspects of cleft repair such as primary healing and establishing optimal form and function of palatal roof and nasal floor.

Polycrystalline Silicon Gettering Layers with Controlled Residual Stress

2013 ◽  
Vol 205-206 ◽  
pp. 284-289 ◽  
Author(s):  
David Lysáček ◽  
Petr Kostelník ◽  
Petr Pánek
Keyword(s):  
Residual Stress ◽  
Vapor Deposition ◽  
Polycrystalline Silicon ◽  
Opposite Sign ◽  
Chemical Vapor ◽  
Pressure Chemical ◽  
Novel Method ◽  
The Individual ◽  
Scanning Electron

We report on a novel method of low pressure chemical vapor deposition of polycrystalline silicon layers used for external gettering in silicon substrate for semiconductor applications. The proposed method allowed us to produce layers of polycrystalline silicon with pre-determined residual stress. The method is based on the deposition of a multilayer system formed by two layers. The first layer is intentionally designed to have tensile stress while the second layer has compressive stress. Opposite sign of the residual stresses of the individual layers enables to pre-determine the residual stress of the gettering stack. We used scanning electron microscopy for structural characterization of the layers and intentional contamination for demonstration of the gettering properties. Residual stress of the layers was calculated from the wafer curvature.

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

10.3791/62045 ◽  
2021 ◽  
Author(s):  
Justin A. Courson ◽  
Paul T. Landry ◽  
Thao Do ◽  
Eric Spehlmann ◽  
Pascal J. Lafontant ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Biological Tissue ◽  
Tissue Samples ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

Field-Emission Scanning Electron Microscopy and Energy-Dispersive X-Ray Analysis to Understand the Role of Tannin-Based Dyes in the Degradation of Historical Wool Textiles

2014 ◽  
Vol 20 (5) ◽  
pp. 1534-1543 ◽  
Author(s):  
Annalaura Restivo ◽  
Ilaria Degano ◽  
Erika Ribechini ◽  
Josefina Pérez-Arantegui ◽  
Maria Perla Colombini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Elemental Composition ◽  
Field Emission ◽  
Cross Sections ◽  
Morphological Changes ◽  
Energy Dispersive ◽  
X Ray ◽  
Edx Analysis ◽  
Scanning Electron

Abstract:An innovative approach, combining field-emission scanning electron microscopy (FESEM) with energy dispersive X-ray spectroscopy (EDX) analysis, is presented to investigate the degradation mechanisms affecting tannin-dyed wool. In fact, tannin-dyed textiles are more sensitive to degradation then those dyed with other dyestuffs, even in the same conservation conditions.FESEM-EDX was first used to study a set of 48 wool specimens (artificially aged) dyed with several raw materials and mordants, and prepared according to historical dyeing recipes. EDX analysis was performed on the surface of wool threads and on their cross-sections. In addition, in order to validate the model formulated by the analysis of reference materials, several samples collected from historical and archaeological textiles were subjected to FESEM-EDX analysis.FESEM-EDX investigations enabled us to reveal the correlation between elemental composition and morphological changes. In addition, aging processes were clarified by studying changes in the elemental composition of wool from the protective cuticle to the fiber core in cross-sections. Morphological and elemental analysis of wool specimens and of archaeological and historical textiles showed that the presence of tannins increases wool damage, primarily by causing a sulfur decrease and fiber oxidation.

scholarly journals Characteristics of Active Carbon from Utilization of Red Chili Trees (Capsicum annuum L)

2019 ◽  
Vol 2 (1) ◽  
pp. 9-13
Author(s):  
Ni Made Dwidiani ◽  
Putu Wijaya Sunu ◽  
Gusti Ngurah Nitya Santhiarsa
Keyword(s):  
Activated Carbon ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Elemental Composition ◽  
Active Carbon ◽  
Chemical Activation ◽  
Morphological Structure ◽  
Testing Procedure ◽  
Scanning Electron ◽  
Made In

This work studies the use of red chilli tree (capsicum anuumm L) waste as material of activated carbon and examines the morphological structure and elemental composition of the activated chili trees. The morphological structure was measured at TekMIRA (Pusat Penelitian dan Pengembangan Teknologi Mineral dan Batubara, Bandung) by using the scanning electron microscope (SEM), and the composition of the elements of carbon, hydrogen, nitrogen and ash is determined by the ultimate testing analysis with the ASTM D5373 standard. In the testing procedure, activated carbon is made from red chili tree waste by dehydration with a temperature of 2000 C for 1 hour and carbonized with a temperature of 3750 C for 1 hour. Then, the chemical activation (NaOH) is made in variation of concentration of 1%, 3%, and 5% with soaked time 24 hours, and dried at 2000 C for one hour. The carbonization at a concentration of 1% (NaOH) gave the best result on activated carbon from red chili trees.

The Characterization of Bovine Bone-Derived Hydroxyapatite Isolated Using Novel Non-Hazardous Method

2020 ◽  
Vol 45 ◽  
pp. 49-56
Author(s):  
Aniek Setiya Budiatin ◽  
Samirah ◽  
Maria Apriliani Gani ◽  
Wenny Putri Nilamsari ◽  
Chrismawan Ardianto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Extraction Method ◽  
Bovine Bone ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ft Ir ◽  
Novel Method ◽  
Scanning Electron

Bovine bone is a considerable source for the production of hydroxyapatite. The recent study reported a novel method to extract hydroxyapatite from bovine bone without producing hazardous residue. The bovine bones were cut and boiled in the opened chamber followed by boiling in pressurized tank. The bones were then soaked into 95% ethanol. Calcination was then conducted in 800°C, 900°C and 1,000°C, for 2 hours. The result was then grinded and sieved. The powder then was characterized using Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) to measure the purity of hydroxyapatite. It is concluded that the hydroxyapatite derived from this process showed 100% purity, resulting 35.34 ± 0.39% w/w from the wet bone weight and 72.3% w/w from the dried weight. The present extraction method has been proven to yield high amount of pure hydroxyapatite as well as reducing the use of hazardous reagent.

A novel method for assessing the role of iron and its functional chelation in fibrin fibril formation: the use of scanning electron microscopy

2013 ◽  
Vol 23 (5) ◽  
pp. 352-359 ◽  
Author(s):  
Etheresia Pretorius ◽  
Natasha Vermeulen ◽  
Janette Bester ◽  
Boguslaw Lipinski ◽  
Douglas B. Kell
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fibril Formation ◽  
Novel Method ◽  
Scanning Electron

Enumeration, location, and characterization of endophytic bacteria within sugar beet roots

1985 ◽  
Vol 63 (7) ◽  
pp. 1262-1265 ◽  
Author(s):  
Mark J. Jacobs ◽  
William M. Bugbee ◽  
David A. Gabrielson
Keyword(s):  
Sugar Beet ◽  
Endophytic Bacteria ◽  
Normal Cell ◽  
Bacterial Population ◽  
Tissue Samples ◽  
Beet Root ◽  
New Zealand White Rabbits ◽  
Sugar Beet Root ◽  
Scanning Electron

An increased bacterial population was found in the secondary root emergence zone tissue of sugar beet roots as compared with core and peripheral tissue of the same beet. The most common bacteria in sugar beet tissue were isolated, characterized, and used to immunize New Zealand white rabbits for antibody production. The bacteria-specific immunoglobulins from the rabbit sera were incubated with sugar beet tissue. These antibody-treated tissues were then incubated with ferritin-labeled goat antirabbit immunoglobulin to assist in identifying immunoglobulin-bound bacteria within tissue samples. This procedure was useful in distinguishing bacteria from artifacts and normal cell inclusions. The location of these antibody-bound bacteria within sugar beet root tissue was identified by using scanning electron microscopy.

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