scholarly journals 24-Hour Measurement of Gastric pH in Rural South Africa

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Alastair M. Sammon ◽  
Eugene J. Ndebia ◽  
Ekambaram Umapathy ◽  
Jehu E. Iputo
Keyword(s):  
South Africa ◽  
Ambulatory Monitoring ◽  
Duodenogastric Reflux ◽  
Lower Oesophageal Sphincter ◽  
Gastric Ph ◽  
Night Time ◽  
High Incidence ◽  
The Mean ◽  
H Pylori ◽  
High Level

Background. Previous studies have established norms of 24-hour gastric pH profiles for western countries. This study was designed to establish the pattern for a rural African population with a high incidence of oesophageal cancer.Methods. After lower oesophageal manometry a probe was placed 10 cm distal to the lower oesophageal sphincter. We carried out 24-hour ambulatory monitoring of gastric pH on 59 healthy subjects. This was satisfactorily completed on 26 female and 18 male (age 21–64, median 35) subjects in the Transkei region of South Africa.Results. The mean 24 hour gastric pH was 2.84 and the mean night-time pH was 3.7. 40 volunteers recorded a night-time pH reaching over 4. 33 volunteers recorded a night-time pH over 7. Night-time alkalinisation was present for 136.4 minutes (25th centile 22.8, 75th centile 208.1) at pH4 or over, and 79.3 (2.5, 122.7) minutes at pH7 or over. Episodes of rapid alkaline rise were 17 (10, 47). 21.1% of these occurred while supine. 35 of 36 tested subjects were positive forH. pyloriIgG.Conclusion. Gastric alkalinisation is common in Transkei, at a higher pH than that reported in other studies, and is sustained longer. Nighttime alkalinisation is frequent. This suggests a high level of duodenogastric reflux.

scholarly journals Genetic Diversity of the cagA gene of Helicobacter pylori strains from Sudanese Patients with Different Gastroduodenal Diseases

2019 ◽  
Author(s):  
Hadeel Gassim Hassan ◽  
Abeer Babiker Idris ◽  
Mohamed A. Hassan ◽  
Hisham N. Altayb ◽  
Kyakonye Yasin ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Amino Acid ◽  
Novel Mutation ◽  
Amino Acid Residues ◽  
Specific Amino Acid ◽  
Gastrointestinal Malignancy ◽  
High Incidence ◽  
H Pylori ◽  
High Level ◽  
Chloride Method

AbstractBackgroundThere is an increase in the prevalence of Helicobacter pylori infection in Sudan, accompanied by a high incidence of upper gastrointestinal malignancy. The cytotoxin-associated gene cagA gene is a marker of a pathogenicity island (PAI) in H. pylori and plays a crucial role in determining the clinical outcome of Helicobacter infections.ObjectiveThis study aimed to determine the frequency and heterogeneity of the cagA gene of H. pylori and correlate the presence of cagA gene with clinical outcomes.Materials and methodsFifty endoscopy biopsies were collected from Fedail and Soba hospitals in Khartoum state. DNA was extracted using the Guanidine chloride method followed by PCR to amplify 16S rRNA and cagA gene of H. pylori using specific primers. DNA amplicons of cagA gene were purified and sequenced. Bioinformatics and statistical analysis were done to characterize and to test the association between cagA gene and gastric complications.ResultsCagA gene was detected in 20/37(54%) of the samples that were found positive for H. pylori. There was no association between endoscopy finding and the presence of the cagA gene (p = 0.225). Specific amino acid variations were found at seven loci related to strains from a patient with duodenitis, gastric ulcer, and gastric atrophy (R448H, T457K, S460L, IT463-464VA, D470E, A482Q, KNV490-491-492TKT) while mutations in cancerous strain were A439P, T457P, and H500Y.ConclusionDisease-specific variations of cagA of H. pylori strains, in the region of amino acid residues 428-510, were evident among Sudanese patients with different gastroduodenal diseases. A novel mutation (K458N) was detected in a patient with duodenitis, which affects the positive electrostatic surface of cagA. Phylogenetic analysis showed a high level of diversity of cagA from Sudanese H. pylori strains.

scholarly journals Ring Shaped Cataract and Urinary Metabolites Among 2,4,6-trinitrotoulene Exposed Population of Pakistan

2021 ◽  
Author(s):  
Sidra Sarwat ◽  
Ambreen Akhtar ◽  
Syeda Fizza Abdud Dayan ◽  
Najma Shaheen ◽  
Humna Shahid Durrani ◽  
...  
Keyword(s):  
Urinary Metabolites ◽  
Control Group ◽  
Spectrometric Analysis ◽  
Urine Metabolites ◽  
Systemic Complications ◽  
Exposed Population ◽  
High Incidence ◽  
The Mean ◽  
High Level ◽  
Control Study

Abstract Background: Safety protocols are usually neglected among most of the TNT exposed population, therefore, rendering the community prone to various occupational hazards. The current study highlights ring shaped cataract and urinary metabolites of TNT among TNT exposed population (n=26) against a control group (n=20).Methods: An observational case-control study was carried out in two groups: 300 subjects exposed to TNT in Dir and Bajour Agency, Pakistan and a control group from the base hospital. We determined the presence of ring shaped cataract and urine metabolites of TNT using slit lamp biomicroscope and gas chromatography mass spectrometric analysis respectively.Results: Results substantiate a high level of urine metabolites for exposed subjects compared to the control group (p<0.001). Age had no significant effect on (p>0.05) on presence of ring shaped cataract and the level of urinary metabolites of TNT, while duration of exposure showed significant effect (p<0.001). Females showed high incidence of ring shaped cataract and urinary metabolites of TNT than men ( p<0.001). The mean age of the exposed subjects was 51±14.38 (Mean+SD) years. The mean year of exposure was 49±5 (Mean+SD) years,Conclusion: This study showed TNT as a risk factor for the presence of ring-shaped cataract among TNT exposed group in Pakistan. It is important to screen exposed community for the presence of ring-shaped cataract, and pre-clinical identification of TNT adducts to prevent systemic complications.

scholarly journals High level of initial default among smear positive pulmonary tuberculosis in eThekwini health district, KwaZulu-Natal

2016 ◽  
Vol 31 (2) ◽  
pp. 41-43
Author(s):  
Lindiwe P. Cele ◽  
Stephen Knight ◽  
Elize Webb ◽  
Khin Tint ◽  
Thembelihle Dlungwane
Keyword(s):  
Infectious Disease ◽  
South Africa ◽  
Pulmonary Tuberculosis ◽  
Case Identification ◽  
Tb Control ◽  
Kwazulu Natal ◽  
High Incidence ◽  
Smear Positive Pulmonary Tuberculosis ◽  
Control Programmes ◽  
High Level

In this study, initial tuberculosis (TB) default was measured in clinics in a district with a high incidence of TB. A retrospective review of TB case identification registers in 2007 found 4049 (9.0%) new smear positive pulmonary tuberculosis (PTB) cases from 44 987 suspects. There were 725 (17.9%) initial defaulters. Fifty-three percent of those traced had already died. TB is the leading cause of death in South Africa due to an infectious disease, and the high level of initial defaults in PTB is one of the “unresolved issues” in TB control programmes that needs to be addressed.

Statistical analysis of distribution, dynamics and etiological structure of bovine diseases in Vologda region

2020 ◽  
pp. 3-4
Author(s):  
Oksana B. Badeeva ◽  
Keyword(s):  
Respiratory Diseases ◽  
Birth Rate ◽  
Statistical Data ◽  
Delayed Diagnosis ◽  
Specific Weight ◽  
Gastrointestinal Diseases ◽  
Vologda Region ◽  
High Incidence ◽  
Young Cattle ◽  
High Level

Statistical data of livestock for 30 years is reflected in the article. Author used the materials of the state veterinary reporting. A comparative analysis of the number, incidence and death rate of adult animals and young cattle for two five-year periods (2001-2005 and 2014-2018). the data of the analysis of veterinary statistical reports for 2018 on the specific weight of the large horned cattle and age dynamics of calves in farms of the Vologda region are shown. A significant decrease in livestock of the large horned cattle by 56.3% (from 1990 to 2018) is shown in the analysis of the data. Over the five years 2014-2018, there was a decrease in the number of the large horned cattle by 31.3%, the birth rate of calves - by 26.2%, and the incidence of calves - by 12.3% and the mortality rate decreased by 3.3%. Despite the decline in the number of livestock, in 2018 there is a high incidence of animal diseases (49.6%). The highest incidence rate was observed among calves under 10 days of age 43.3%, 31.7% - from 11 to 30 days, 15.8% - from one to three months, 6.5% - from three to six months and 2.7% - from 6 to 12 months. Of the total number of sick calves in 2018, 63.2% had gastrointestinal diseases, and death for this reason is 49.6% of the total number of victims. Respiratory diseases affect 21.8% of young animals, and death due to respiratory diseases is 18.2%. Analysis of statistical data showed that, despite the complex of veterinary and sanitary measures, the incidence and death of calves remain at a high level. This can be explained by delayed diagnosis and low therapeutic effectiveness in gastrointestinal and respiratory diseases of cattle.

DFT Benchmark Study of the O--O Bond Dissociation Energy in Peroxides Validated with High-Level Ab-Initio Calculations

2019 ◽  
Author(s):  
Danilo Carmona ◽  
Pablo Jaque ◽  
Esteban Vöhringer-Martinez
Keyword(s):  
Reference Value ◽  
Basis Set ◽  
Basis Sets ◽  
Mean Deviation ◽  
Bond Dissociation ◽  
Dissociation Energies ◽  
The Mean ◽  
Experimental Values ◽  
High Level ◽  
Almost All

<div><div><div><p>Peroxides play a central role in many chemical and biological pro- cesses such as the Fenton reaction. The relevance of these compounds lies in the low stability of the O–O bond which upon dissociation results in radical species able to initiate various chemical or biological processes. In this work, a set of 64 DFT functional-basis set combinations has been validated in terms of their capability to describe bond dissociation energies (BDE) for the O–O bond in a database of 14 ROOH peroxides for which experimental values ofBDE are available. Moreover, the electronic contributions to the BDE were obtained for four of the peroxides and the anion H2O2− at the CBS limit at CCSD(T) level with Dunning’s basis sets up to triple–ζ quality provid- ing a reference value for the hydrogen peroxide anion as a model. Almost all the functionals considered here yielded mean absolute deviations around 5.0 kcal mol−1. The smallest values were observed for the ωB97 family and the Minnesota M11 functional with a marked basis set dependence. Despite the mean deviation, order relations among BDE experimental values of peroxides were also considered. The ωB97 family was able to reproduce the relations correctly whereas other functionals presented a marked dependence on the chemical nature of the R group. Interestingly, M11 functional did not show a very good agreement with the established order despite its good performance in the mean error. The obtained results support the use of similar validation strategies for proper prediction of BDE or other molecular properties by DF Tmethods in subsequent related studies.</p></div></div></div>

The way of value of Correlation of genomic DNA microsatellite loci and live weight of Chukchi reindeer

2020 ◽  
pp. 27-32
Author(s):  
V. Dodokhov ◽  
N. Pavlova ◽  
T. Rumyantseva ◽  
L. Kalashnikova
Keyword(s):  
Microsatellite Markers ◽  
Agricultural Production ◽  
Live Weight ◽  
Biodiversity Loss ◽  
Tribal Community ◽  
High Germination ◽  
Wide Range ◽  
The Mean ◽  
High Level ◽  
Dna Microsatellite

The article presents the genetic characteristic of the Chukchi reindeer breed. The object of the study was of the Chukchi reindeer. In recent years, the number of reindeer of the Chukchi breed has declined sharply. Reduced reindeer numbers could lead to biodiversity loss. The Chukchi breed of deer has good meat qualities, has high germination viability and is adapted in adverse tundra conditions of Yakutia. Herding of the Chukchi breed of deer in Yakutia are engaged only in the Nizhnekolymsky district. There are four generic communities and the largest of which is the agricultural production cooperative of nomadic tribal community «Turvaurgin», which was chosen to assess the genetic processes of breed using microsatellite markers: Rt6, BMS1788, Rt 30, Rt1, Rt9, FCB193, Rt7, BMS745, C 143, Rt24, OheQ, C217, C32, NVHRT16, T40, C276. It was found that microsatellite markers have a wide range of alleles and generally have a high informative value for identifying of genetic differences between animals and groups of animal. The number of identified alleles is one of the indicators of the genetic diversity of the population. The total number of detected alleles was 127. The Chukchi breed of deer is characterized by a high level of heterozygosity, and the random crossing system prevails over inbreeding in the population. On average, there were 7.9 alleles (Na) per locus, and the mean number of effective alleles (Ne) was 4.1. The index of fixation averaged 0.001. The polymorphism index (PIC) ranged from 0.217 to 0.946, with an average of 0.695.

The Occurrence of Legionella Bacteria in Cooling Towers in South Africa

1991 ◽  
Vol 24 (2) ◽  
pp. 149-152 ◽  
Author(s):  
N. A. Grabow ◽  
E. J. Pienaar ◽  
R. Kfir
Keyword(s):  
South Africa ◽  
Most Probable Number ◽  
Cooling Towers ◽  
Probable Number ◽  
Service Water ◽  
Standard Plate ◽  
Viable Bacteria ◽  
Plate Counts ◽  
High Level ◽  
Biocide Treatment

A total of 510 service water samples from cooling towers throughout South Africa were analysed for the presence of Legionella bacteria. Legionella was detected using an immuno-labelling technique based on the most probable number principle. Only cultural (viable) bacteria were counted. Legionellae were found in most of the samples tested. However, in only 4% of the samples a high level of legionellae was recorded. No correlation was found between the numbers of legionellae and those of standard plate counts. Biocide treatment was shown to be effective in the removal of the bacteria from cooling towers after a 3-month treatment period.

scholarly journals Comparison of Propofol and Thiopentone Anaesthesia (with Special Reference to Recovery Characteristics)

1987 ◽  
Vol 15 (4) ◽  
pp. 389-393 ◽  
Author(s):  
W. M. Weightman ◽  
M. Zacharias
Keyword(s):  
Blood Pressure ◽  
Systolic Blood Pressure ◽  
Day Case ◽  
Early Recovery ◽  
Day Case Surgery ◽  
Pain On Injection ◽  
High Incidence ◽  
Case Surgery ◽  
Repeated Doses ◽  
The Mean

Thiopentone and propofol were used for the induction and maintenance of anaesthesia in unpremedicated patients undergoing minor gynaecological procedures. There were no significant differences in the induction and maintenance characteristics except for a high incidence of pain on injection and a greater fall in the mean systolic blood pressure associated with propofol in comparison with thiopentone. Propofol was associated with a quicker early recovery as well as a faster psychomotor recovery, as tested by a peg-board. However, complete psychomotor recovery was not achieved for up to three hours in some patients receiving propofol and so caution is advised regarding the early street fitness of patients receiving repeated doses of the drug for day case surgery.

scholarly journals Siyabonga: a humble hero making a difference with his hands… and his heart

2021 ◽  
pp. bjsports-2021-104118
Author(s):  
Jon Patricios
Keyword(s):  
South Africa ◽  
Significant Contribution ◽  
Patellofemoral Pain ◽  
Nelson Mandela ◽  
Making A Difference ◽  
High Level ◽  
Professional Capacity

Siyabonga Kunene’s athletic build and confident stance belie his humble roots. Born in an impoverished area of South Africa, he soon learnt the benefits of an education. He is now a PhD-qualified sports physiotherapist making a significant contribution in both academic and clinical realms. Remaining true to his origins and the inspirational words of Nelson Mandela, Siyabonga has created a framework for managing patellofemoral pain in under-resourced athletes. He has established himself as a physiotherapy lecturer at his university and has been appointed to national teams in his professional capacity. However, his focus primary remains on treating those with a passion for sport who would not normally be able to access high-level athletic care.

scholarly journals AB0189 LIPOPROTEIN ABNORMALITIES IN RHEUMATOID ARTHRITIS PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1119.1-1119
Author(s):  
L. Nacef ◽  
Y. Besbes ◽  
Y. Mabrouk ◽  
H. Ferjani ◽  
K. Maatallah ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Demographic Data ◽  
Calcium Score ◽  
Healthy Person ◽  
Mean Value ◽  
Immunological Parameters ◽  
American College Of Rheumatology ◽  
Patient Consent ◽  
The Mean ◽  
High Level

Background:The lipid paradox is termed the decreased cholesterol level in rheumatoid arthritis (RA). Nevertheless, the apolipoprotein levels are usually higher than a healthy person and are predictors of cardiovascular events.Objectives:We aimed to describe lipid abnormalities in RA patients and to look for predictor factors of these changes.Methods:The prospective study was carried out on patients with RA who met the 2010 American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) classification criteria. These patients were followed in the rheumatology department of the Kassab Institute.We collected the socio-demographic data, biological and immunological parameters.The lipid assessment included: a measurement of total cholesterol (TC), HDL, LDL, and triglycerides (TG). Lipoproteins APOA1 and APOB were measured. All data were collected after patient consent.Results:Of the 47 patients recruited, 78.7% were female. The mean age was 52.5 ±11.06 [32-76]. The average RA progressed from 86.25 ±63 months [5-288] and was erosive in 81.6% of cases. The rheumatoid factor (RF) was positive in 57.8% of patients, and citrullinated antipeptide antibodies (ACPA) were present in 62.2%. Eight patients had a previous CV history.Mean TC was 4.42 ±1.3 [1.2-7.58], mean HDL was 1.38 ±0.73 [0.18-4.10], mean LDL was 2.55 ±1.16 [0.24-5.54]. The mean TG value was 1.28 ±0.6 [0.24-5.54]. TC elevation was found in 9.1% of cases, HDL in 21.3% of cases, LDL in 5.5% of cases, and TG in 16.4% of cases. Mean APOB/APOA1 ratio was 0.67 ±0.18 [0,46-1,11]. LDL elevation was associated to a high DAS28 (p=0.06, r=0.512). APOA1 was associated to a low DAS28 (p=0.04, r=-0.642).The mean value of APO A1 was 1.36 ±0.21 [0.84-1.81], that of APOB was 0.90 ±0.22 [0.58-1.40]. APOA1 values were lower in patients with high-level LDL (p=0.767). The APOB value was associated with lipid disturbance without significant correlation (p=0.291).Conclusion:Lipid test abnormalities can be found in RA patients outside of any known CV risk factors. APOA1 seems to have a protective effect. Screening and treatment of these abnormalities can prevent CV risk.References:[1]Miguel Bernardes and al. Coronary artery calcium score in female rheumatoid arthritis patients: Associations with apolipoproteins and disease biomarkers. Int J Rheum Dis. 2019;00:1–16.[2]Anna So dergren and al. Biomarkers associated with cardiovascular disease in patients with early rheumatoid arthritis. PLOS ONE. August 5, 2019.Disclosure of Interests:None declared

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