scholarly journals Inhibitory Effect ofLoranthus parasiticuson IgE-Mediated Allergic Responses in RBL-2H3 Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jae-Myung Yoo ◽  
Ju-Hye Yang ◽  
Young Soo Kim ◽  
Won-Kyung Cho ◽  
Jin Yeul Ma
Keyword(s):  
Phenolic Compounds ◽  
Late Phase ◽  
Water Extract ◽  
Dry Weight ◽  
Interleukin 4 ◽  
Total Phenolic ◽  
Prostaglandin E ◽  
Dependent Manner ◽  
Antiallergic Activity ◽  
Ige Mediated

The mistletoeLoranthus parasiticushas been used as a compound for traditional medicine in Northeast Asia for a long time and is known to possess neuroprotective action. Nonetheless, the effect ofLoranthus parasiticuson allergic responses remains unknown. In the present study, we evaluated whether the water extract ofLoranthus parasiticus(LPE) could inhibit IgE-mediated allergic responses in RBL-2H3 cells. LPE inhibited the release ofβ-hexosaminidase (IC50, 184.5 μg/mL) and the formation of tumor necrosis factor-α(IC50, 84.27 μg/mL), interleukin-4 (IC50, 93.43 μg/mL), prostaglandin E2(IC50, 84.10 μg/mL), prostaglandin D2, and leukotriene C4(IC50, 43.27 μg/mL) in a concentration-dependent manner. Moreover, LPE inhibited phosphorylation of Syk, PLCγ1/2, PKCδ, ERK, JNK, p38, and Akt. In the late phase, LPE decreased 5-lipoxygenase phosphorylation and COX-2 expression but not cPLA2phosphorylation. Additionally, LPE included total phenolic compounds (10.72 mg/g dry weight) and total flavonoids (56.20 mg/g dry weight). These results suggest that the phenolic compounds or flavonoids contained in LPE may be associated with antiallergic activity. The phenolic compounds and flavonoids in LPE are antiallergic phytochemicals capable of inhibiting the activation of the FcεRI signaling cascade in mast cells. Such effects may provide further information for the development of a phytomedicine for allergic diseases.

scholarly journals Effects of Light-Emitting Diodes on the Accumulation of Glucosinolates and Phenolic Compounds in Sprouting Canola (Brassica napus L.)

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 76 ◽  
Author(s):  
Chang Park ◽  
Nam Kim ◽  
Jong Park ◽  
Sook Lee ◽  
Jong-Won Lee ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Phenolic Compounds ◽  
Light Emitting Diode ◽  
Red Light ◽  
Dry Weight ◽  
Total Phenolic ◽  
Brassica Napus L ◽  
Light Emitting ◽  
Led Light ◽  
Sprout Growth

In this study, we investigated optimal light conditions for enhancement of the growth and accumulation of glucosinolates and phenolics in the sprouts of canola (Brassica napus L.). We found that the shoot lengths and fresh weights of red light-irradiated sprouts were higher than those of sprouts exposed to white, blue, and blue + red light, whereas root length was not notably different among red, blue, white, and blue + red light treatments. The accumulations of total glucosinolates in plants irradiated with white, blue, and red lights were not significantly different (19.32 ± 0.13, 20.69 ± 0.05, and 20.65 ± 1.70 mg/g dry weight (wt.), respectively). However, sprouts exposed to blue + red light contained the lowest levels of total glucosinolates (17.08 ± 0.28 mg/g dry wt.). The accumulation of total phenolic compounds was the highest in plants irradiated with blue light (3.81 ± 0.08 mg/g dry wt.), 1.33 times higher than the lowest level in plants irradiated with red light (2.87 ± 0.05 mg/g dry wt.). These results demonstrate that red light-emitting diode (LED) light is suitable for sprout growth and that blue LED light is effective in increasing the accumulation of glucosinolates and phenolics in B. napus sprouts.

The Effect of Heating Temperature on Total Phenolic Content, Antioxidant Activity, and Phenolic Compounds of Plum and Mahaleb Fruits

2019 ◽  
Vol 15 (11-12) ◽  
Author(s):  
Kashif Ghafoor ◽  
Isam A. Mohamed Ahmed ◽  
Süleyman Doğu ◽  
Nurhan Uslu ◽  
Gbemisola J. Fadimu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Heating Temperature ◽  
Dry Weight ◽  
Total Phenolic ◽  
Dihydroxybenzoic Acid ◽  
Different Temperatures

AbstractThe effect of heating at different temperatures (60, 80, 90, 110, and 130 °C) on the total phenolic content, antioxidant activity, and phenolic compounds present in plum and mahaleb fruits was investigated. The antioxidant activity values and total phenolic contents of fresh plum (93.82% measured by DPPH method, 787.79 mg gallic acid equivalent (GAE)/100 g dry weight determined by Folin method) and mahaleb fruits (81.80%, 634.47 mg GAE/100 g dry weight) were higher than plum and mahaleb fruits dried at different temperatures (p < 0.05). Generally, the heating process caused a reduction in both total phenolic content and antioxidant activity for plum and mahaleb. While (+)-catechin (92.62 mg/kg), 1,2-dihydroxybenzene (132.15 mg/kg), gallic acid (107.01 mg/kg), and 3,4-dihydroxybenzoic acid (74.59 mg/kg) are the key phenolic compounds in fresh plum, (+)-catechin, 3,4-dihydroxybenzoic acid, 1,2-dihydroxybenzene, and syringic and caffeic acids were the major phenolic compounds of mahaleb fruits. The polyphenol content of fruits and the class of phenolics present are significantly affected by heating temperature.

scholarly journals Variety and Year Impact on Phenolic Content of Arkansas-Grown Sweetpotato Leaves

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 371-371
Author(s):  
Josue Bolanos ◽  
Sun-Ok Lee ◽  
Luke Howard ◽  
Cindi Brownmiller ◽  
Shahidul Islam ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Statistical Significance ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antioxidant Potential ◽  
Total Phenolic

Abstract Objectives Sweetpotato leaf (SPL) is a natural source of phenolic compounds with potential utility as an antioxidant. The study aimed to measure the impacts of the years on SPL total phenolic content and antioxidant capacity and to identify and quantify the individual phenolic compounds. Methods Sweetpotato leaves in 2018 (27 varieties) and 2019 (24 varieties) were grinded and lyophilized. Phenolic compounds were extracted with 70% ethanol from SPLs. Total phenolic content was determined by Folin-Ciocalteu method and antioxidant potential was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Five SPL samples with statistically highest antioxidant capacity were identified and quantified by HPLC. All statistical analyses were carried out by SAS software using ANOVA. Statistical significance was accepted at P &lt; 0.05. Results The average of total phenolic contents in 2018 SPL samples was 43 ± 13 mg gallic acid equivalent (GAE)/g dry weight (DW) SPL whereas in 2019, it was 53 ± 9 mg GAE/g dry weight SPL. Antioxidant capacity in 2018 was 110 ± 75 µmol Trolox equivalent (TE)/g dry weight SPL whereas in 2019, it was 132 ± 32 µmol TE/g dry weight SPL. SPL1 had the highest antioxidant potential, followed by SPL9, SPL3, SPL28, and SPL11 (P &lt; 0.05). The concentrations of major identified phenolics from the five SPL samples (#1, 9, 3, 28, and 11) in dry weight of SPL were: chlorogenic acid at 3.05 ± 0.35 mg/g, 5-monocaffeoylquinic acid (CQA) at 0.71 ± 0.06 mg chlorogenic acid equivalent (ChAE)/g, 4-CQA at 0.99 ± 0.07 mg ChAE/g, 3,4-diCQA at 1.22 ± 0.18 mg ChAE/g, 3,4,5-triCQA at 1.2 ± 0.18 mg ChAE/g, and 3,5-diCQA at 15.5 ± 4.05 mg ChAE/g. 3,5-diCQA, the predominant phenolic, was present in the highest amounts in SPL1. Conclusions The results showed that sweetpotato leaves collected in 2019 contained higher total phenolics and antioxidant capacity than the ones collected in 2018. Phenolic compounds have strong antioxidant activity in Arkansas-grown sweetpotato leaves. This study warrants further investigation of sweetpotato leaves to be utilized as an antioxidant. Funding Sources The work was supported by USDA-NIFA.

Utilization of kudzu as a lead phytoremediator and the impact of lead on selected physiological responses

2013 ◽  
Vol 93 (5) ◽  
pp. 951-959 ◽  
Author(s):  
Kristin Schwarzauer-Rockett ◽  
Safaa H. Al-Hamdani ◽  
James R. Rayburn ◽  
Nixon O. Mwebi
Keyword(s):  
Phenolic Compounds ◽  
Stomatal Conductance ◽  
Lead Concentration ◽  
Ethylenediaminetetraacetic Acid ◽  
Physiological Responses ◽  
Dry Weight ◽  
Control Treatment ◽  
Total Phenolic ◽  
Plant Responses ◽  
The Impact

Schwarzauer-Rockett, K., Al-Hamdani, S. H., Rayburn, J. R. and Mwebi, N. O. 2013. Utilization of kudzu as a lead phytoremediator and the impact of lead on selected physiological responses. Can. J. Plant Sci. 93: 951–959. This study was carried out to evaluate kudzu [Pueraria montana var. lobata (Willd.)] in lead phytoremediation. The impact of lead (PbNO3) concentrations of 100 and 200 mg L−1 on plant growth, photosynthetic pigments, photosynthesis, and stomatal conductance were evaluated. Additionally, concentrations of phenolic compounds and anthocyanin were determined. The lead was dissolved in 0.142 g L−1 ethylenediaminetetraacetic acid (EDTA) and an EDTA control treatment was added to evaluate the impact of EDTA on the above selected plant responses. Root accumulation of lead was significantly higher than in the shoot. Kudzu growth in response to the presence of lead and EDTA in the Hoagland's solution was similar to that of the EDTA control, except for plants growing at 200 mg L−1, which showed significantly lower root dry weight. Total phenolic compounds increased with the presence of EDTA and lead in the Hoagland's solution. Photosynthetic rate, stomatal conductance, chlorophyll a and chlorophyll b, carotenoids, and anthocyanin were not different (P≤0.05) among the treatments, with the exception that carotenoids were significantly higher in plants growing in the presence of EDTA without lead compared with 200 mg L−1 lead concentration. Kudzu accumulated most of the lead in the root and therefore can be considered as a rhizofiltrator. As kudzu was able to accumulate 1.02% (wt/wt) of lead it can be considered a hyper-accumulator.

scholarly journals Estimation of total tannins, total phenolics and total flavonoids of different extracts of cedrela toona roxb. Fruits.

2021 ◽  
Vol 10 (1) ◽  
pp. 05-10
Author(s):  
Shah Kinjal H ◽  
Patel Piyush M.
Keyword(s):  
Tannic Acid ◽  
Colorimetric Assay ◽  
Water Extract ◽  
Soxhlet Extraction ◽  
Dry Weight ◽  
Powdered Material ◽  
Aluminium Chloride ◽  
Assay Method ◽  
Total Phenolic ◽  
Total Flavonoids

Air dried powdered material of the fruits of cedrela toona Roxb. was successively extracted with petroleum ether, hexane, acetone, methanol and water extract by soxhlet extraction and subjected to various qualitative chemical tests to determine presence of various phytoconstituents like alkaloids, glycosides, carbohydrates, phenolics and tannins, phtosterols, fixed oils and fats, proteins, amino acids, flavonoids, saponins etc. Total flavonoids content was measured with the ‘Aluminium Chloride Colorimetric Assay Method’. Total Phenolic content in the aqueous and methanol extracts of fruits of Cedrela toona Roxb. were determined using the Folin Denis Method and expressed as mg of tannic acid equivalents per gram dry weight of extract. This study helps researchers for developmentof isolation method of active ingredient having vast pharmacological effects. Keywords: Cedrela toona, total flavonoids, total tannins and phenolics, Tannic acid

scholarly journals Accumulation of Phenolic Compounds and Glucosinolates in Sprouts of Pale Green and Purple Kohlrabi (Brassica oleracea var. gongylodes) under Light and Dark Conditions

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1939
Author(s):  
Ramaraj Sathasivam ◽  
Min Cheol Kim ◽  
Hyeon Ji Yeo ◽  
Bao Van Nguyen ◽  
Soo In Sohn ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Brassica Oleracea ◽  
Root Length ◽  
Dry Weight ◽  
Light Treatment ◽  
Total Phenolic ◽  
Light Conditions ◽  
Fresh Weight ◽  
Dark Conditions ◽  
Pale Green

Kohlrabi is considered an important dietary vegetable worldwide. In this study, we investigated the growth and accumulation of phenolic compounds (PCs) and glucosinolates in sprouts of pale green and purple kohlrabi (Brassica oleracea var. gongylodes) in response to light and dark conditions. Pale green kohlrabi presented high fresh weight and root length irrespective of light treatment, whereas under dark conditions, it presented higher fresh weight and shoot length than purple kohlrabi. In contrast, the root length of both kohlrabies increased markedly under light conditions compared to that under dark conditions. Thirteen PCs and eight glucosinolates were detected and quantified in 10-day-old pale green and purple kohlrabies. In both kohlrabies, the individual and total phenolic levels were much higher under the light treatment than under the dark treatment. Under light and dark conditions, the total phenolic content was 6362.13 and 5475.04 µg/g dry weight in the pale green kohlrabi, respectively, whereas in the purple kohlrabi, it was 10,115.76 and 9361.74 µg/g dry weight, respectively. Dark conditions favored higher accumulation of glucosinolates than light conditions. Progoitrin, neoglucobrassicin, glucoerucin, and 4-methoxyglucobrassicin were the predominant glucosinolates in both kohlrabies and were present in much higher amounts in the pale green kohlrabi. In pale green kohlrabi under dark conditions, the total glucosinolates content was 4.75 and 2.62 times higher than that of the purple kohlrabi under light and dark conditions, respectively. Among individual glucosinolates, in the pale green kohlrabi under the dark condition, progoitrin was found to have the highest content, which was 90.28 and 54.51 times higher than that in the purple kohlrabi under light and dark conditions, respectively. These results show that the phenolic and glucosinolates levels varied widely, and these variations between the two types of kohlrabi under both light and dark conditions were significant. Our findings suggest that light and dark conditions enhance the accumulation of PCs and glucosinolates, respectively, during the development of kohlrabi seedlings.

scholarly journals Comparative Study on Phenolic Compounds and Antioxidant Activities of Hop (Humulus lupulus L.) Strobile Extracts

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 135
Author(s):  
Jae Il Lyu ◽  
Jaihyunk Ryu ◽  
Kyoung-Sun Seo ◽  
Kyung-Yun Kang ◽  
Sang Hoon Park ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Flavonoid Compound ◽  
Total Phenolic ◽  
Extraction Solvent

In this study, we investigated the phenolic compounds in hop strobile extracts and evaluated their antioxidant property using DPPH and ABTS assay. The total phenolic compound (TPC) and total flavonoid compound (TFC) estimated in two different solvent extracts considerably varied depending on the extraction solvent. The most abundant phenolic compound in hop strobile was humulones (α-acid) with levels ranging from 50.44 to 193.25 µg/g. El Dorado accession revealed higher antioxidant activity in ethanol extracts (DPPH: IC50 124.3 µg/mL; ABTS: IC50 95.4 µg/mL) when compared with that of the other accessions. Correlations between DPPH (IC50) scavenging TFC in ethanol extract (TFC_E, −0.941), and TPC_E (−0.901), and between ABTS (IC50) scavenging TFC_E (−0.853), and TPC_E (−0.826), were statistically significant at p < 0.01 level, whereas no significant correlation was observed between antioxidant activities, TPC and TFC in water extract. This study is the first to report that variations in the level of phenolic contents and antioxidant activity of various hop cultivars depended on the type of extraction solvent used and the cultivation regions. These results could provide valuable information on developing hop products.

scholarly journals Pilot Scale Elimination of Phenolic Cellulase Inhibitors From Alkali Pretreated Wheat Straw for Improved Cellulolytic Digestibility to Fermentable Saccharides

2021 ◽  
Vol 9 ◽  
Author(s):  
Ikram ul Haq ◽  
Ali Nawaz ◽  
Badar Liaqat ◽  
Yesra Arshad ◽  
Xingli Fan ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Wheat Straw ◽  
Phenolic Content ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Pilot Scale ◽  
Total Phenolic ◽  
Saccharification Yield ◽  
Pre Treatment ◽  
Pretreated Wheat Straw

Depleting supplies of fossil fuel, regular price hikes of gasoline and environmental deterioration have necessitated the search for economic and eco-benign alternatives of gasoline like lignocellulosic biomass. However, pre-treatment of such biomass results in development of some phenolic compounds which later hinder the depolymerisation of biomass by cellulases and seriously affect the cost effectiveness of the process. Dephenolification of biomass hydrolysate is well cited in literature. However, elimination of phenolic compounds from pretreated solid biomass is not well studied. The present study was aimed to optimize dephenoliphication of wheat straw using various alkalis i.e., Ca(OH)2 and NH3; acids i.e., H2O2, H2SO4, and H3PO4; combinations of NH3+ H3PO4 and H3PO4+ H2O2 at pilot scale to increase enzymatic saccharification yield. Among all the pretreatment strategies used, maximum reduction in phenolic content was observed as 66 mg Gallic Acid Equivalent/gram Dry Weight (GAE/g DW), compared to control having 210 mg GAE/g DW using 5% (v/v) combination of NH3+H3PO4. Upon subsequent saccharification of dephenoliphied substrate, the hydrolysis yield was recorded as 46.88%. Optimized conditions such as using 1%+5% concentration of NH3+ H3PO4, for 30 min at 110°C temperature reduced total phenolic content (TPC) to 48 mg GAE/g DW. This reduction in phenolic content helped cellulases to act more proficiently on the substrate and saccharification yield of 55.06% was obtained. The findings will result in less utilization of cellulases to get increased yield of saccharides by hydrolyzing wheat straw, thus, making the process economical. Furthermore, pilot scale investigations of current study will help in upgrading the novel process to industrial scale.

scholarly journals Germinated Riceberry Rice Enhanced Protocatechuic Acid and Vanillic Acid to Suppress Melanogenesis through Cellular Oxidant-Related Tyrosinase Activity in B16 Cells

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 247 ◽  
Author(s):  
Teerapat Rodboon ◽  
Seiji Okada ◽  
Prasit Suwannalert
Keyword(s):  
Phenolic Compounds ◽  
Vanillic Acid ◽  
Protocatechuic Acid ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Mushroom Tyrosinase ◽  
Tyrosinase Inhibition ◽  
Dose Dependent

The anti-melanogenic bioactivities of phytophenolic compounds have been well recognized. Riceberry rice contains a rich source of phenolic compounds that act as melanin inhibitors through their antioxidant and anti-tyrosinase properties. Germination has been shown to be an effective process to improve targeted phenolic compounds. In this study, germinated riceberry rice extract was tested for antioxidant activity. Total phenolic content was determined while the tyrosinase inhibitory effect was screened by the in vitro mushroom tyrosinase assay. Cytotoxicity of germinated riceberry rice extract was investigated in B16 cells before evaluating its activities on cellular tyrosinase, melanogenesis, melanin excretion, morphological appearance, and cellular oxidants. Germinated riceberry rice extract showed increased potency of antioxidants and was also twice as effective for mushroom tyrosinase inhibition when compared with ungerminated riceberry rice extract. In B16 cells, the extract inhibited cellular tyrosinase, melanogenesis, and cellular oxidants in a dose-dependent manner when compared with untreated cells. Germinated riceberry rice extract also displayed an effect on B16 cells morphology by reducing the number of melanin- containing cells and their dendriticity. Additionally, the germination of riceberry rice dominantly enhanced two phenolic acids, protocatechuic acid and vanillic acid, which have the potential for antioxidant-associated hyperpigmentation control. Thus, the restricted germination of riceberry rice tended to promote protocatechuic acid and vanillic acid, which dominantly displayed antioxidants and tyrosinase-related melanogenic inhibition.

scholarly journals Germinated Buckwheat: Effects of Dehulling on Phenolics Profile and Antioxidant Activity of Buckwheat Seeds

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 740
Author(s):  
Andrej Živković ◽  
Tomaž Polak ◽  
Blaž Cigić ◽  
Tomaž Požrl
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Functional Foods ◽  
Phenolic Content ◽  
Germination Rate ◽  
Dry Weight ◽  
Total Phenolic ◽  
Negative Effects ◽  
Germinated Seeds

The aim was to investigate the effects of the cold dehulling of buckwheat seeds on their germination, total phenolic content (TPC), antioxidant activity (AA) and phenolics composition. Cold dehulling had no negative effects on germination rate and resulted in faster rootlet growth compared to hulled seeds. Although the dehulling of the seeds significantly decreased TPC and AA, the germination of dehulled seeds resulted in 1.8-fold and 1.9-fold higher TPC and AA compared to hulled seeds. Liquid chromatography coupled to mass spectrometry identified several phenolic compounds in free and bound forms. Rutin was the major compound in hulled seeds (98 µg/g dry weight), orientin and vitexin in 96-h germinated dehulled seeds (2205, 1869 µg/g dry weight, respectively). During germination, the increases in the major phenolic compounds were around two orders of magnitude, which were greater than the increases for TPC and AA. As well as orientin and vitexin, high levels of other phenolic compounds were detected for dehulled germinated seeds (e.g., isoorientin, rutin; 1402, 967 µg/g dry weight, respectively). These data show that dehulled germinated seeds of buckwheat have great potential for use in functional foods as a dietary source of phenolic compounds with health benefits.

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