scholarly journals Production of a Functional Frozen Yogurt Fortified with Bifidobacterium spp.

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Amro Abdelazez ◽  
Zafarullah Muhammad ◽  
Qiu-Xue Zhang ◽  
Zong-Tao Zhu ◽  
Heba Abdelmotaal ◽  
...  
Keyword(s):  
Sensory Evaluation ◽  
Bifidobacterium Longum ◽  
Bifidobacterium Bifidum ◽  
Lactobacillus Delbrueckii ◽  
Bifidobacterium Infantis ◽  
Bifidobacterium Adolescentis ◽  
Physiochemical Properties ◽  
The People ◽  
Significant Difference ◽  
Frozen Yogurt

Frozen dairy products have characteristics of both yogurt and ice cream and could be the persuasive carriers of probiotics. Functions of the frozen yogurt containing viable bifidobacterial cells are recognized and favored by the people of all ages. We developed a kind of yogurt supplemented by Bifidobacterium species. Firstly, five strains of Bifidobacterium spp. (Bifidobacterium bifidum ATCC 11547, Bifidobacterium longum ATCC 11549, Bifidobacterium infantis ATCC 11551, Bifidobacterium adolescentis ATCC 11550, and Bifidobacterium breve ATCC 11548) were evaluated based on the feasibility criteria of probiotics, comprising acid production, bile tolerance, and adhesion to epithelial cells. Formerly, we combined the optimum strains with yogurt culture (Lactobacillus delbrueckii subsp. bulgaricus EMCC 11102 and Streptococcus salivarius subsp. thermophilus EMCC 11044) for producing frozen yogurt. Finally, physiochemical properties and sensory evaluation of the frozen yogurt were investigated during storage of 60 days at −18°C. Results directed that Bifidobacterium adolescentis ATCC 11550 and Bifidobacterium infantis ATCC 11551 could be utilized with yogurt culture for producing frozen yogurt. Moreover, the frozen yogurt fermented by two bifidobacterial strains and yogurt culture gained the high evaluation in the physiochemical properties and sensory evaluation. In summary, our results revealed that there was no significant difference between frozen yogurt fermented by Bifidobacterium spp. and yogurt culture and that fermented by yogurt culture only.

scholarly journals Isolation of a Bifidogenic Peptide from the Pepsin Hydrolysate of Bovine Lactoferrin

2013 ◽  
Vol 79 (6) ◽  
pp. 1843-1849 ◽  
Author(s):  
Hirotsugu Oda ◽  
Hiroyuki Wakabayashi ◽  
Koji Yamauchi ◽  
Takumi Sato ◽  
Jin-Zhong Xiao ◽  
...  
Keyword(s):  
Bifidobacterium Longum ◽  
Amino Acid Mixture ◽  
Bifidobacterium Bifidum ◽  
Acid Mixture ◽  
Bovine Lactoferrin ◽  
Bifidobacterium Adolescentis ◽  
Content Type ◽  
Active Peptide ◽  
Representative Species ◽  
Pepsin Hydrolysate

ABSTRACTLactoferrin is an iron-binding glycoprotein found in the milk of most mammals for which various biological functions have been reported, such as antimicrobial activity and bifidogenic activity. In this study, we compared the bifidogenic activity of bovine lactoferrin (bLF) and pepsin hydrolysate of bLF (bLFH), isolated bifidogenic peptide from bLFH, and investigated the bifidogenic spectra of bLF, bLFH, and its active peptide against 42 bifidobacterial strains comprising nine species. AgainstBifidobacterium breveATCC 15700T, minimal effective concentrations of bLF and bLFH were 300 and 10 μg/ml. AgainstBifidobacterium longumsubsp.infantisATCC 15697T, the minimal effective concentration of bLFH was 30 μg/ml, and bLF did not show bifidogenic activity within 300 μg/ml. As an active peptide, a heterodimer of A1-W16and L43-A48linked by a disulfide bond was isolated. Previously, this peptide was identified as having antibacterial activity. An amino acid mixture with the same composition as this peptide showed no bifidogenic activity. The strains of each species whose growth was highly promoted (>150%) by this peptide at 3.75 μM were as follows:B. breve(7 out of 7 strains [7/7]),B. longumsubsp.infantis(5/5),Bifidobacterium bifidum(2/5),B. longumsubsp.longum(1/3),Bifidobacterium adolescentis(3/6),Bifidobacterium catenulatum(1/4),Bifidobacterium pseudocatenulatum(0/4),Bifidobacterium dentium(0/5), andBifidobacterium angulatum(0/3). Growth of none of the strains was highly promoted by bLF at 3.75 μM. We demonstrated that bLFH showed stronger bifidogenic activity than natural bLF, especially against infant-representative species,B. breveandB. longumsubsp.infantis; furthermore, we isolated its active peptide. This is the first report about a bifidogenic peptide derived from bLF.

scholarly journals ОСОБЕННОСТИ ПОЛУЧЕНИЯ СИНБИОТИЧЕСКОГОКИСЛОМОЛОЧНОГО ПРОДУКТА НА ОСНОВЕКОНСОРЦИУМА БИФИДОБАКТЕРИЙ

2019 ◽  
Author(s):  
Е.П. Каменская ◽  
М.В. Обрезкова ◽  
Е.Е. Базеева
Keyword(s):  
Bifidobacterium Longum ◽  
Bifidobacterium Bifidum ◽  
Bifidobacterium Adolescentis

Одним из приоритетных направлений пищевых технологий является создание продуктов, обладающих лечебно-профилактическим и функциональным действием, в частности кисломолочных симбиотических продуктов, сочетающих в себе сложные компоненты направленного действия – пробиотики и пребиотики. Цель работы – исследование взаимодействия штаммов различных видов бифидобактерий для получения на их основе консорциума с новыми биотехнологическими свойствами и создание синбиотического кисломолочного продукта с высоким титром бифидобактерий. Объектами исследований были выбраны штаммы Bifidobacterium bifidum 791, Bifidobacterium longum 379 М, Bifidobacterium adolescentis МС-42. При оценке качества в работе применяли общепринятые методы исследований. Установлено, что оптимальным соотношением штаммов B. bifidum 791, B. longum 379 М, B. аdolescentis МС-42 в составе комбинированной закваски является 1:1:2. Диско-диффузионным методом проведен анализ устойчивости консорциума к 23 антибиотикам разных поколений и спектров действий. Наибольшая устойчивость отмечена к бета-лактамным антибиотикам, бактерицидная активность которых обусловлена ингибированием синтеза клеточной стенки микроорганизмов. Также изучено влияние различных концентраций пребиотиков полисахаридной природы (пектина и инулина) на изменение количества жизнеспособных клеток бифидобактерий. Показано, что синбиотический кисломолочный продукт на основе подобранного консорциума бифидобактерий с внесением 2,5 % пектина может быть рекомендован для профилактики и коррекции нарушений дисбаланса кишечной микрофлоры, а также поддержания неспецифической резистентности организма. 

scholarly journals Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract

2009 ◽  
Vol 75 (6) ◽  
pp. 1534-1545 ◽  
Author(s):  
Francesca Turroni ◽  
Elena Foroni ◽  
Paola Pizzetti ◽  
Vanessa Giubellini ◽  
Angela Ribbera ◽  
...  
Keyword(s):  
Bifidobacterium Longum ◽  
Bifidobacterium Bifidum ◽  
Selective Medium ◽  
Rrna Gene ◽  
Bifidobacterium Adolescentis ◽  
Fecal Samples ◽  
Bifidobacterium Animalis ◽  
Human Intestinal Microbiota ◽  
Health Promoting ◽  
Internally Transcribed Spacer

ABSTRACT Although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. We performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rRNA gene sequences (16S rRNA gene and internally transcribed spacer [ITS] 16S-23S spacer sequences) of isolated colonies. A total of 900 isolates were collected, of which 704 were shown to belong to bifidobacteria. Analyses showed that the culturable bifidobacterial population from intestinal and fecal samples include six main phylogenetic taxa, i.e., Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bifidobacterium adolescentis, Bifidobacterium pseudolongum, Bifidobacterium breve, and Bifidobacterium bifidum, and two species mostly detected in fecal samples, i.e., Bifidobacterium dentium and Bifidobacterium animalis subp. lactis. Analysis of bifidobacterial distribution based on age of the subject revealed that certain identified bifidobacterial species were exclusively present in the adult human gut microbiota whereas others were found to be widely distributed. We encountered significant intersubject variability and composition differences between fecal and mucosa-adherent bifidobacterial communities. In contrast, a modest diversification of bifidobacterial populations was noticed between different intestinal regions within the same individual (intrasubject variability). Notably, a small number of bifidobacterial isolates were shown to display a wide ecological distribution, thus suggesting that they possess a broad colonization capacity.

scholarly journals Lactulose ingestion causes an increase in the abundance of gut-resident bifidobacteria in Japanese women: a randomised, double-blind, placebo-controlled crossover trial

2021 ◽  
pp. 1-12
Author(s):  
Y. Sakai ◽  
H. Hamano ◽  
H. Ochi ◽  
F. Abe ◽  
K. Masuda ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Bacterial Species ◽  
Bifidobacterium Longum ◽  
Illumina Miseq ◽  
Japanese Women ◽  
Bifidobacterium Bifidum ◽  
Control Group ◽  
Double Blind ◽  
Bifidobacterium Adolescentis ◽  
Cell Counts

The genus Bifidobacterium comprises various bacterial species, and the complement of species within the human intestinal tract differs from individual to individual. The balance of these bifidobacterial species remains poorly understood, although it is known that the abundance of bifidobacteria increases following the ingestion of prebiotics. We previously conducted a randomised, placebo-controlled, double-blind, crossover study of 2 g/day lactulose ingestion for 2 weeks in 60 Japanese women. To study the effect of lactulose ingestion on each bifidobacterial species, here, we measured the abundance of each of the principal bifidobacterial species. After lactulose ingestion, the log cell counts of the Bifidobacterium adolescentis group (8.97±0.08 vs 9.39±0.08, P=0.0019), Bifidobacterium catenulatum group (9.45±0.10 vs 9.65±0.10, P=0.0032) and Bifidobacterium longum group (9.01±0.07 vs 9.29±0.07, P=0.0012) were significantly higher than in the placebo ingestion control group. However, the log cell counts were similar for Bifidobacterium breve (8.12±0.12 vs 8.33±0.12, P=0.20), Bifidobacterium bifidum (9.08±0.12 vs 9.42±0.14, P=0.095) and Bifidobacterium animalis subspecies lactis (8.65±0.53 vs 8.46±0.46, P=0.77). Cluster analysis of the log cell count data at the bifidobacterial species level revealed three distinct clusters, but the combinations and ratios of the constituent bifidobacteria were not affected by lactulose ingestion. Furthermore, principal coordinate analysis of the intestinal microbiota in the lactulose and placebo ingestion groups using Illumina MiSeq showed no significant differences in the intestinal microbiota as a whole. These results suggest that 2 g/day lactulose ingestion for 2 weeks significantly increases the abundance of intestinal bifidobacteria, but does not affect the intestinal microbiota as a whole.

Investigating bifidobacteria and human milk oligosaccharide composition of lactating mothers

2020 ◽  
Vol 96 (5) ◽  
Author(s):  
Gabriele Andrea Lugli ◽  
Sabrina Duranti ◽  
Christian Milani ◽  
Leonardo Mancabelli ◽  
Francesca Turroni ◽  
...  
Keyword(s):  
Human Milk ◽  
Intestinal Tract ◽  
Bifidobacterium Longum ◽  
Bifidobacterium Bifidum ◽  
Metagenomic Data ◽  
Bifidobacterium Adolescentis ◽  
Milk Samples ◽  
Flow Cytometric ◽  
Lactating Mothers

ABSTRACT Human milk is known to carry its own microbiota, of which the precise origin remains obscure. Breastfeeding allows mother-to-baby transmission of microorganisms as well as the transfer of many other milk components, such as human milk oligosaccharides (HMOs), which act as metabolizable substrates for particular bacteria, such as bifidobacteria, residing in infant intestinal tract. In the current study, we report the HMO composition of 249 human milk samples, in 163 of which we quantified the abundance of members of the Bifidobacterium genus using a combination of metagenomic and flow cytometric approaches. Metagenomic data allowed us to identify four clusters dominated by Bifidobacterium adolescentis and Bifidobacterium pseudolongum, Bifidobacterium crudilactis or Bifidobacterium dentium, as well as a cluster represented by a heterogeneous mix of bifidobacterial species such as Bifidobacterium breve and Bifidobacterium longum. Furthermore, in vitro growth assays on HMOs coupled with in silico glycobiome analyses allowed us to elucidate that members of the Bifidobacterium bifidum and B. breve species exhibit the greatest ability to degrade and grow on HMOs. Altogether, these findings indicate that the bifidobacterial component of the human milk microbiota is not strictly correlated with their ability to metabolize HMOs.

scholarly journals Functional Probiotic Yoghurt with Spirulina

2019 ◽  
Vol 38 (04) ◽  
Author(s):  
Rita Narayana ◽  
Asaram Kale
Keyword(s):  
Bifidobacterium Bifidum ◽  
Sampling Time ◽  
Point Of View ◽  
Lactobacillus Delbrueckii ◽  
Streptococcus Salivarius ◽  
Bioactive Substances ◽  
Dairy Foods ◽  
Significant Difference ◽  
High Degree ◽  
Made In

An attempt has been made in the present study to explore the potential of Spirulina, a cyanobacterium, photoautotrophic microorganism in initiating a stimulatory effect on the microflora of Probiotic yoghurt. Probiotic yoghurt was prepared by adding 1 percent inoculum of probiotic Bifidobacterium bifidum to yoghurt cultures viz., Streptococcus. salivarius ssp. thermophilus and Lactobacillus. delbrueckii ssp. bulgaricus. Spirulina enriched functional Probiotic yoghurt was prepared by using 1 gram of Spirulina per litre of mix.The pH and acidity of probiotic yoghurt and Spirulina enriched Probiotic yoghurt on 0 day was 4.31± 0.007, 0.96± 0.002 and 4.31± 0.009, 0.96± 0.005 respectively. On the 3rd it was 4.30 ± 0.003, 0.96 ± 0.004 and 4.31 ± 0.004, 0.96± 0.005 respectively. There was no significant difference in the pH and acidity of Probiotic and Spirulina enriched yoghurt between these 2 sampling periods. The pH and acidity of probiotic yoghurt and spirulina enriched probiotic yoghurt on the 7th day was 4.28 ± 0.001, 1.11 ± 0.030 and 4.31 ± 0.004, 1.02 ± 0.023 respectively. Significant difference was noticed in pH and acidity in these two treatments on 7th day. The Spirulina enriched sample was less acidic than Probiotic yoghurt. There was virtually no difference in viable numbers of S. salivarius ssp. thermophilus, L. delbrueckii ssp. bulgaricus and Bifidobacterium bifidum on 0 and 3rd day. However the growth of the three lactic acid bacteria used was higher in Spirulina enriched yoghurt than in Probiotic yoghurt on the 7th day. The addition of cyanobacterial bio mass to Bifidobacterium bifidum, S. salivarius ssp. thermophilus and L. delbrueckii ssp. bulgaricus had beneficial effect on their viability. No spoilage organism was detected at any sampling time, indicating the high degree of sanitation during processing and packaging products. Thus the abundance of bioactive substances in Spirulina is of great importance from a nutritional point of view as it provides new opportunity for the manufacture of functional dairy foods.

Bifidobacterial strains shared by mother and child as source of probiotics

2018 ◽  
Vol 9 (2) ◽  
pp. 231-238 ◽  
Author(s):  
A. Peirotén ◽  
J.L. Arqués ◽  
M. Medina ◽  
E. Rodríguez-Mínguez
Keyword(s):  
Breast Milk ◽  
Bifidobacterium Longum ◽  
Bifidobacterium Bifidum ◽  
Special Role ◽  
Bifidobacterium Adolescentis ◽  
Probiotic Potential ◽  
Gastrointestinal Conditions ◽  
Mother And Child ◽  
Mode Of Birth

Importance of bifidobacteria as part of the infant intestinal microbiota has been highlighted. Their acquisition is influenced by the mode of birth and the feed regime afterwards, with a special role of the maternal microbiota. The presence of the same shared bifidobacterial strains between breast milk and infant faeces in 14 mother-infant pairs was assessed by means of pulsed-field gel electrophoresis (PFGE) genotyping. Four shared strains of Bifidobacterium breve (2), Bifidobacterium longum subsp. infantis and B. longum subsp. longum were found in breast milk-infant faeces pairs. Two years later, a second survey yielded four shared strains of the species Bifidobacterium adolescentis, Bifidobacterium bifidum, B. longum subsp. longum and Bifidobacterium pseudocatenulatum. Moreover, a B. bifidum strain was found to be shared by the infant faeces of the first study and the mother faeces tested two years later, pointing out a long term persistence. Some of the selected bifidobacterial strains showed probiotic potential due to their survival to gastrointestinal conditions and their ability to form biofilms.

scholarly journals Effect of inulin on the human gut microbiota: stimulation ofBifidobacterium adolescentisandFaecalibacterium prausnitzii

2008 ◽  
Vol 101 (4) ◽  
pp. 541-550 ◽  
Author(s):  
Carlett Ramirez-Farias ◽  
Kathleen Slezak ◽  
Zoë Fuller ◽  
Alan Duncan ◽  
Grietje Holtrop ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bifidobacterium Longum ◽  
Control Period ◽  
Bifidobacterium Bifidum ◽  
Bifidobacterium Adolescentis ◽  
Food Ingredients ◽  
Bifidobacterium Animalis ◽  
Bacterial Groups

Prebiotics are food ingredients that improve health by modulating the colonic microbiota. The bifidogenic effect of the prebiotic inulin is well established; however, it remains unclear which species ofBifidobacteriumare stimulatedin vivoand whether bacterial groups other than lactic acid bacteria are affected by inulin consumption. Changes in the faecal microbiota composition were examined by real-time PCR in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in comparison with a control period without any supplement intake. The prevalence of most bacterial groups examined did not change after inulin intake, although the low G+C % Gram-positive speciesFaecalibacterium prausnitziiexhibited a significant increase (10·3 % for control periodv.14·5 % during inulin intake,P = 0·019). The composition of the genusBifidobacteriumwas studied in four of the volunteers by clone library analysis. Between three and fiveBifidobacteriumspp. were found in each volunteer.Bifidobacterium adolescentisandBifidobacterium longumwere present in all volunteers, andBifidobacterium pseudocatenulatum,Bifidobacterium animalis,Bifidobacterium bifidumandBifidobacterium dentiumwere also detected. Real-time PCR was employed to quantify the four most prevalentBifidobacteriumspp.,B. adolescentis,B. longum,B. pseudocatenulatumandB. bifidum, in ten volunteers carrying detectable levels of bifidobacteria.B. adolescentisshowed the strongest response to inulin consumption, increasing from 0·89 to 3·9 % of the total microbiota (P = 0·001).B. bifidumwas increased from 0·22 to 0·63 % (P < 0·001) for the five volunteers for whom this species was present.

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