scholarly journals In Vitro and Ex Vivo Chemopreventive Action of Mauritia flexuosa Products

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Joilane Alves Pereira-Freire ◽  
George Laylson da Silva Oliveira ◽  
Layana Karine Farias Lima ◽  
Carla Lorena Silva Ramos ◽  
Stella Regina Arcanjo-Medeiros ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ex Vivo ◽  
Peroxyl Radicals ◽  
Palm Tree ◽  
Mauritia Flexuosa ◽  
Before And After ◽  
Scavenger Action ◽  
Ruminant Animals ◽  
By Products

Mauritia flexuosa (Arecaceae), known as “Buriti,” is a Brazilian palm tree with high economic potential for local communities. Herein, we investigated the phytochemistry profile and antioxidant potential of M. flexuosa fruits and determined the bioaccessibility of phenolic compounds. Peels revealed upper values for phenols, flavonoids, carotenoids, tannins, and ascorbic acid when compared to the pulps and endocarps. All samples showed capacity to scavenger free radicals (0.5, 1.0, 2.0, 4.0, and 8.0 mg/mL) but peels presented higher scavenger action in all methods explored. Phenolic compounds identified by HPLC displayed reduced bioaccessibility after in vitro simulated gastrointestinal digestion for pulp (38.7%), peel (18.7%), and endocarp (22.3%) extracts (P<0.05). Buriti fruits also protected rat blood cells against lysis induced by peroxyl radicals. We demonstrated the promising chemopreventive potentialities of M. flexuosa fruits and their by-products and peels with higher quantities of bioactive compounds and phenolic substances before and after in vitro bioaccessibility investigation. In Brazil, these parts are discarded or underused, mainly as feed for ruminant animals. Consequently, it is extremely important to explore nutritional characteristics of these by-products for human/livestock foods and to install biofriendly techniques and sustainable biotechnology handling of natural resources.

A comparative study of phenolic compounds profile and in vitro antioxidant activity from buriti (Mauritia flexuosa) by-products extracts

LWT ◽  
2021 ◽  
pp. 111941
Author(s):  
Adenilson Renato Rudke ◽  
Kátia Suzana Andrade ◽  
Simone Mazzutti ◽  
Acácio Antônio Ferreira Zielinski ◽  
Victor Rodrigues Alves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Comparative Study ◽  
Mauritia Flexuosa ◽  
By Products ◽  
In Vitro Antioxidant Activity

Assessment of in-vitro bioaccessibility and antioxidant capacity of phenolic compounds extracts recovered from grapevine bunch stem and cane by-products

2021 ◽  
Vol 348 ◽  
pp. 129063
Author(s):  
Susana Ferreyra ◽  
Carolina Torres-Palazzolo ◽  
Rubén Bottini ◽  
Alejandra Camargo ◽  
Ariel Fontana
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
In Vitro Bioaccessibility ◽  
By Products

scholarly journals Evaluation of Agro-Industrial by Products as Potential Local Feed for Ruminant Animals: Chemical Composition, Fiber Fractions and In Vitro Rumen Fermentation

2019 ◽  
Vol 20 (3) ◽  
pp. 155
Author(s):  
Said Mirza Pratama ◽  
Sitti Wajizah ◽  
Anuraga Jayanegara ◽  
Samadi Samadi
Keyword(s):  
Chemical Composition ◽  
Animal Feed ◽  
Rumen Fermentation ◽  
Coffee Pulp ◽  
Fiber Fractions ◽  
Ruminant Animals ◽  
Coconut Meal ◽  
By Products ◽  
In Vitro Rumen Fermentation

Animals are considered as source of protein should be improved their productivity with the minimum cost production. Agro-industrial by products have been used as animal feed to reduce feed cost. The purpose of this study is to evaluate agro-industrial by product in Aceh as potential local feed for ruminant animals based on chemical composition, fiber fraction and in vitro rumen fermentation. There were eight sources of agro-industrial by products (sago residues, coconut meal, soybean-ketchup by product, coffee pulp, cacao pod, sago tree, corncob, and rice brand) which were collected from 3 different locations in Aceh.  All agro-industrial by product samples were dried at 600C for 24 h and ground to pass a 1 mm sieve. Grounded samples were analyzed to determine chemical composition, fiber fractions and in vitro rumen fermentation. Incubation was conducted at temperature 390C for 48 h in water bath with three replicates. Data for in vitro rumen fermentation were statically calculated by using SPSS differences between treatments were stated (P≤0.05) by using Duncan Multiple Range Test (DMRT). The results indicated that agro industrial by product from coconut meal, ketchup residues, coffee by product, cacao by product, and rice brand can be used as source of protein and industrial by product from sago by product, sago tree and corncob can be used as source of energy. Neutral detergent  insoluble  CP (NDICP) and Acid detergent insoluble CP (ADICP)  were relatively high for sago by product, sago tree and rice brand but relatively low for cacao by product and corncob. The value of incubated pH for most feed samples was in the normal range. In vitro dry matter digestibility (IVDMD) and in vitro organic matter  digestibility (IVOMD) were significantly difference (P≤0.05) each agro-industrial by products with the highest for sago and the lowest for coffee by product. In conclusion, agro-industrial by products had a potential feed for ruminant animals both as protein and energy sources. However, feed ingredients with high fiber content and low degradability, further treatments such as physical, chemical and biological treatments were required to improve the feed quality. 

scholarly journals The Diverse Potential of Gluten from Different Durum Wheat Varieties in Triggering Celiac Disease: A Multilevel In Vitro, Ex Vivo and In Vivo Approach

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3566
Author(s):  
Federica Gaiani ◽  
Sara Graziano ◽  
Fatma Boukid ◽  
Barbara Prandi ◽  
Lorena Bottarelli ◽  
...  
Keyword(s):  
Immune Response ◽  
Celiac Disease ◽  
Durum Wheat ◽  
Ex Vivo ◽  
Diet Group ◽  
Control Group ◽  
Wheat Varieties ◽  
Before And After

The reasons behind the increasing prevalence of celiac disease (CD) worldwide are still not fully understood. This study adopted a multilevel approach (in vitro, ex vivo, in vivo) to assess the potential of gluten from different wheat varieties in triggering CD. Peptides triggering CD were identified and quantified in mixtures generated from simulated gastrointestinal digestion of wheat varieties (n = 82). Multivariate statistics enabled the discrimination of varieties generating low impact on CD (e.g., Saragolla) and high impact (e.g., Cappelli). Enrolled subjects (n = 46) were: 19 healthy subjects included in the control group; 27 celiac patients enrolled for the in vivo phase. Celiacs were divided into a gluten-free diet group (CD-GFD), and a GFD with Saragolla-based pasta group (CD-Sar). The diet was followed for 3 months. Data were compared between CD-Sar and CD-GFD before and after the experimental diet, demonstrating a limited ability of Saragolla to trigger immunity, although not comparable to a GFD. Ex vivo studies showed that Saragolla and Cappelli activated immune responses, although with great variability among patients. The diverse potential of durum wheat varieties in triggering CD immune response was demonstrated. Saragolla is not indicated for celiacs, yet it has a limited potential to trigger adverse immune response.

scholarly journals Inhibition of Stimulated Interleukin-2 Production in Whole Blood: A Practical Measure of Cyclosporine Effect

1999 ◽  
Vol 45 (9) ◽  
pp. 1477-1484 ◽  
Author(s):  
C Michael Stein ◽  
John J Murray ◽  
Alastair JJ Wood
Keyword(s):  
Whole Blood ◽  
Ex Vivo ◽  
Interleukin 2 ◽  
Major Effect ◽  
Biologically Relevant ◽  
Maximum Inhibition ◽  
Before And After ◽  
Dose Dependent Fashion ◽  
The Relationship

Abstract Background: Prediction of cyclosporine (CSA) efficacy and toxicity in individual patients is difficult. There is no practical, biologically relevant, pharmacodynamic measure of CSA effect. A major effect of CSA is to decrease interleukin-2 (IL-2) production; however, measurement of this effect in isolated lymphocytes as a marker of response to CSA has been problematic. Methods: CSA inhibition of phytohemagglutinin-P (PHA)-stimulated IL-2 production, measured by ELISA, was studied ex vivo in whole blood drawn before, and after subjects received 4 mg/kg oral CSA. Results: Four hours after CSA was administered, the mean (± SD) CSA concentration was 702 ± 196 μg/L and PHA-stimulated IL-2 production decreased by 68.7% ± 17.2% (P &lt;0.0001; n = 17). Twenty-four hours after CSA was administered, concentrations were low (64 ± 24 μg/L), with no inhibition of IL-2 production. A rapid, concentration-dependent response occurred. Maximum CSA concentrations (944 ± 187 μg/L) and maximum inhibition of IL-2 production (86.9% ± 13.7%) occurred 90 min after subjects received CSA. In vitro, 32.5–1200 μg/L CSA also inhibited PHA-stimulated IL-2 production in whole blood in a dose-dependent fashion with a similar IC50 (∼300–400 μg/L) ex vivo and in vitro. Conclusion: In the search for a pharmacodynamic marker to better guide immunosuppressive therapy, the relationship between this simple, biologically relevant measure of CSA effect and clinical outcome should be determined.

scholarly journals Applicability of an In-Vitro Digestion Model to Assess the Bioaccessibility of Phenolic Compounds from Olive-Related Products

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6667
Author(s):  
Patricia Reboredo-Rodríguez ◽  
Carmen González-Barreiro ◽  
Elena Martínez-Carballo ◽  
Noelia Cambeiro-Pérez ◽  
Raquel Rial-Otero ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Structural Changes ◽  
Virgin Olive Oil ◽  
In Vitro Digestion ◽  
Current Status ◽  
Human Organism ◽  
Olive Leaves ◽  
Functional Value ◽  
Before And After

The Mediterranean diet includes virgin olive oil (VOO) as the main fat and olives as snacks. In addition to providing nutritional and organoleptic properties, VOO and the fruits (olives) contain an extensive number of bioactive compounds, mainly phenolic compounds, which are considered to be powerful antioxidants. Furthermore, olive byproducts, such as olive leaves, olive pomace, and olive mill wastewater, considered also as rich sources of phenolic compounds, are now valorized due to being mainly applied in the pharmaceutical and nutraceutical industries. The digestive system must physically and chemically break down these ingested olive-related products to release their phenolic compounds, which will be further metabolized to be used by the human organism. The first purpose of this review is to provide an overview of the current status of in-vitro static digestion models for olive-related products. In this sense, the in-vitro gastrointestinal digestion methods are widely used with the following aims: (i) to study how phenolic compounds are released from their matrices and to identify structural changes of phenolic compounds after the digestion of olive fruits and oils and (ii) to support the functional value of olive leaves and byproducts generated in the olive industry by assessing their health properties before and after the gastrointestinal process. The second purpose of this review is to survey and discuss all the results available to date.

scholarly journals Evaluation of Nutritive Values of Agro-Industrial By-Products (AIBPS) and Effect of Their Nano Structural Characteristics on the Kinetics of Biogas Production and Methane Emission

2021 ◽  
Author(s):  
Shahram Shirmohammadi ◽  
Akbar Taghizadeh ◽  
Hamid Paya ◽  
Arash Javanmard ◽  
Soheila Abachi ◽  
...  
Keyword(s):  
Methane Emission ◽  
Biogas Production ◽  
Ex Vivo ◽  
Structural Characteristics ◽  
In Vitro Digestibility ◽  
Fermentation Parameters ◽  
Experimental Treatments ◽  
Kinetics Of ◽  
By Products

Abstract Over the past decades, the agro-industrial by-products (AIBP) has received considerable attention. With this motivation, the aim of this study was to investigate the effect of AIBP as a source of non-fiber carbohydrates on biogas production kinetic, methane emission and fermentation characteristics. Experimental treatments were (1) Sugar beet pulp (SBP) (control), (2) Apple pomace (AP), (3) Orange pulp (OP, (4) 33% AP + 66% OP, (5) 50% AP + 50% OP and (6) 66% AP + 33%OP. For this work, we analyzed the data collected from the kinetics of digestion through biogas production, ex-vivo methane emission, in-vitro digestibility of dry matter and fermentation parameters. Field emission scanning electron microscope was used to show the nano structural differences of the AIBPs. Our results demonstrated the significant differences of the crude protein among the treatments (P < 0.05). Biogas production and methane emission were significantly higher in SBP and OP treatments (P < 0.05). The most eminent and the lowest amounts of acetate were observed for AP and OP (61.84 mmol/L, 58.15 mmol/L), respectively. More broken edges were obvious in OP images. particle size was rather smaller in SBP. Images of AP showed a sleek surface which may act as a shield preventing more digestion. Overall, beside reducing environmental contamination by AIBP, our results showed a positive effect of AIBPs on degradation and biogas kinetics, methane emission and in vitro fermentation parameters describing that they can be used as a good source of non-fiber energy sources.

Neutralization of Enoxaparine-lnduced Bleeding by Protamine Sulfate

1990 ◽  
Vol 63 (02) ◽  
pp. 271-274 ◽  
Author(s):  
J Van Ryn-McKenna ◽  
L Cai ◽  
F A Ofosu ◽  
J Hirsh ◽  
M R Buchanan
Keyword(s):  
Blood Loss ◽  
Unfractionated Heparin ◽  
Ex Vivo ◽  
Factor Xa ◽  
Protamine Sulfate ◽  
Low Molecular Weight Heparins ◽  
Before And After ◽  
Made In

SummaryIt has been suggested that protamine sulfate is a poor antidote for the bleeding side-effeets of low molecular weight heparins (LMWHs) in vivo, since protamine sulfate does not completely neutralize the anti-factor Xa activity of LMWHs in vitro or ex vivo. Therefore, we performed experiments to compare directly the abilities of protamine sulfate to neutralize the anticoagulant activities of the LMWH, enoxaparine, and unfractionated heparin ex vivo, with its ability to neutralize the bleeding side-effeets of both compounds in vivo. Bleeding was measured as the amount of blood lost from 5 cuts made in rabbits ears before and after treatment with enoxaparine or unfractionated heparin ± protamine sulfate. Plasma anti-factor Xa and anti-thrombin activities ex vivo, were measured chromogenically. Doses of 400 and 1,500 anti-factor Xa U/kg of heparin and enoxaparine, respectively, were required to enhance blood loss to the same extent. Protamine sulfate completely neutralized blood loss induced by both compounds, but did not neutralize the anti-factor Xa nor antithrombin activities ex vivo. We conclude that protamine sulfate is an effective antidote for the bleeding side-effeets of enoxaparine and unfractionated heparin, despite its inability to completely neutralize their anticoagulant activities.

Lipocortin-1 distribution in bronchoalveolar lavage from healthy human lung: effect of prednisolone

1995 ◽  
Vol 79 (1) ◽  
pp. 121-128 ◽  
Author(s):  
S. F. Smith ◽  
T. D. Tetley ◽  
A. K. Datta ◽  
T. Smith ◽  
A. Guz ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bronchoalveolar Lavage ◽  
Ex Vivo ◽  
Specific Cell ◽  
Surface Binding ◽  
Healthy Human ◽  
Before And After ◽  
The Difference ◽  
Specific Cell Surface

Lipocortin-1 (LC-1; annexin-1) may mediate some anti-inflammatory actions of the glucocorticoids, probably after binding to specific cell surface binding sites. We have quantified LC-1 levels in bronchoalveolar lavage (BAL) fluid and cells collected from seven healthy volunteers before and after 7 days of treatment with an oral glucocorticoid, prednisolone (30 mg/day). Extracellular BAL LC-1 was higher and cellular LC-1 was lower after prednisolone than before [extracellular: before, median 98 ng/mg albumin (range 48–350 ng/mg albumin); after, 236 ng/mg albumin (19–414 ng/mg albumin); P < 0.05. Cellular: before, 23.3 ng/10(6) cells (14.6–26.9 ng/10(6) cells); after, 18.0 ng/10(6) cells (122–268 ng/10(6) cells); P < 0.05]. The distribution of LC-1 within BAL cells ex vivo (cell surface = 25%, cytosol = 50%, membrane = 25%) was unaffected by prednisolone treatment. However, in adherent cells that had been cultured for 4 h, 70–80% of the LC-1 was on the cell surface. In summary, prednisolone appears to promote cellular release of LC-1. The difference in distribution of cellular LC-1 in BAL cells ex vivo and in vitro may reflect adherence and/or activation.

scholarly journals Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Simon Schroeder ◽  
Christin Mache ◽  
Hannah Kleine-Weber ◽  
Victor M. Corman ◽  
Doreen Muth ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Middle East ◽  
Ex Vivo ◽  
Transcriptional Profiling ◽  
Phenotypic Change ◽  
Knock Out ◽  
Immune Genes ◽  
Before And After ◽  
Pre Treatment

AbstractMiddle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels across the Middle East and Africa. Virus-induced pneumonia in humans results from animal contact, with a potential for limited onward transmission. Phenotypic changes have been suspected after a novel recombinant clade (lineage 5) caused large nosocomial outbreaks in Saudi Arabia and South Korea in 2016. However, there has been no functional assessment. Here we perform a comprehensive in vitro and ex vivo comparison of viruses from parental and recombinant virus lineages (lineage 3, n = 7; lineage 4, n = 8; lineage 5, n = 9 viruses) from Saudi Arabia, isolated immediately before and after the shift toward lineage 5. Replication of lineage 5 viruses is significantly increased. Transcriptional profiling finds reduced induction of immune genes IFNB1, CCL5, and IFNL1 in lung cells infected with lineage 5 strains. Phenotypic differences may be determined by IFN antagonism based on experiments using IFN receptor knock out and signaling inhibition. Additionally, lineage 5 is more resilient against IFN pre-treatment of Calu-3 cells (ca. 10-fold difference in replication). This phenotypic change associated with lineage 5 has remained undiscovered by viral sequence surveillance, but may be a relevant indicator of pandemic potential.

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