scholarly journals Preliminary Study on the Effect of Bacillus amyloliquefaciens TL on Cecal Bacterial Community Structure of Broiler Chickens

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Yuxuan Hong ◽  
Yingxian Cheng ◽  
Yanjuan Li ◽  
Xiaowen Li ◽  
Zutao Zhou ◽  
...  
Keyword(s):  
Growth Performance ◽  
Bacillus Amyloliquefaciens ◽  
Broiler Chickens ◽  
High Throughput Sequencing ◽  
Control Diet ◽  
Probiotic Strain ◽  
Feed Additive ◽  
Control Group ◽  
Feed Conversion ◽  
Cecal Microbiota

Probiotics can promote the health and growth performance of animals through modulation of intestinal microbiota. When used as a feed additive, they have the potential to minimize or abolish the use of antibiotics. In this study, we investigated the effect of the probiotic strain Bacillus amyloliquefaciens TL on the growth performance and cecum microflora composition in Cobb 500 broiler chickens. In total, 180 broilers were randomly divided into three groups—each group comprised 4 pens, and each pen contained 15 chickens. The three groups were fed either a control diet, or a diet supplemented with either the antibiotic chlortetracycline or B. amyloliquefaciens TL. Broilers were weighed, and cecum contents were collected on days 7, 14, 21, and 35, respectively. The broilers in both the antibiotic and probiotic groups exhibited significant weight gain compared with controls, exhibiting increases of 16.02% and 13.40%, respectively, after 35 days (P<0.01). Similarly, the feed conversion ratio (FCR, 1–35 days) of broilers in the chlortetracycline and B. amyloliquefaciens TL groups was lower than that of the controls. HiSeq high-throughput sequencing of 16S rRNA of the cecal microbiota was performed on days 7, 14, 21, and 35, respectively. The Firmicutes/Bacteroidetes ratio was higher in the chlortetracycline and B. amyloliquefaciens TL groups than in the control group on days 14, 21, and 35, and especially on day 21. The prevalence of genera Oscillospira, Ruminococcus, Butyricicoccus, and Faecalibacterium (Firmicutes) was higher in the antibiotic and probiotic groups, while that of Bacteroides, Parabacteroides (Bacteroidetes), and Lactobacillus was higher in the control group. In this study, the changes in the microbiota of the probiotic group were similar to those in the antibiotic group. These results suggest that the probiotic strain B. amyloliquefaciens TL can modulate the cecal microbiota of broilers similar to chlortetracycline.

scholarly journals Efficacy of a Synergistic Blend of Organic Acids and ß-1,4 Mannobiose on Cecal Salmonella Counts and Growth Performance in Salmonella Challenged Broiler Chickens: A Meta-Analysis

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2988
Author(s):  
Sandra J. A. van Kuijk ◽  
Yanming Han
Keyword(s):  
Organic Acids ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Meta Analysis ◽  
Control Diet ◽  
Conversion Ratio ◽  
Feed Additive ◽  
Control Group ◽  
Post Inoculation ◽  
Feed Conversion

This study aimed at investigating the effect of a functional synergistic feed additive blend, containing organic acids and ß-1,4 mannobiose, on cecal Salmonella counts and growth performance of broiler chickens. A meta-analysis combining 13 individual studies, executed in Salmonella-challenged broilers comparing a control diet with and without the synergistic blend, was performed. Cecal Salmonella colonies and overall growth performance were measured. Raw data from all studies were combined and analyzed using PROC MIXED in SAS, taking the within and between-study variation into account. In the first 14 days post-inoculation (DPI), cecal Salmonella was reduced by 0.429 log CFU/g (p = 0.011, n = 10 comparisons from five studies) when feeding the synergistic blend compared to the control group. During 15–34 DPI, the overall means were not different between treatments (0.069 log CFU/g; p = 0.519, n = 12 comparisons from eight studies). The feed conversion ratio was improved when feeding the synergistic blend compared to the control diet (1.474 vs. 1.482, respectively; p = 0.002). In conclusion, feeding a synergistic blend, containing organic acids and ß-1,4 mannobiose amongst other ingredients, reduced cecal Salmonella counts during the first 14 DPI and improved growth performance of Salmonella challenged broilers compared to a control diet.

scholarly journals Effect of Nanosilica and Bentonite as Mycotoxins Adsorbent Agent in Broiler Chickens’ Diet on Growth Performance and Hepatic Histopathology

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2129
Author(s):  
Abdallah A. Ghazalah ◽  
Mamduh O. Abd-Elsamee ◽  
Kout Elkloub M. E. Moustafa ◽  
Mohamed Abdelrazik Khattab ◽  
Abd-Elrahim A. A. Rehan
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Control Diet ◽  
Dietary Treatment ◽  
Control Group ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Four Levels ◽  
Hepatic Histopathology

Mycotoxins are toxic secondary metabolites produced by different strains of fungi, such as aspergillus, fusarium, and penicillium that can contaminate feed ingredients or the entire feed of poultry and animals. Mycotoxins can cause many serious complications to both humans and animals due to carcinogenic, mutagenic, and immunosuppressive disorders. Therefore, the present experiment aims to investigate the effect of broiler chickens’ diets supplemented with different levels of nanosilica (NS) as an adsorbent agent of mycotoxins on their growth performance and hepatic histopathology. Detectable levels of toxins were present in the feed before feeding, and all levels of mycotoxins were above the normal limit. A total of 180 one-day-old male Arbor Acres broiler chickens were allocated randomly to six treatment groups with three replicates per group, including ten chickens per replicate. The experiment lasted for five weeks, and dietary treatments included control diet and diets with four levels of nanosilica as 0.05%, 0.10%, 0.15%, and 0.20% as well as 0.50% bentonite (fixfin® Dry) diet. Bodyweight, body weight gain, average daily feed intake, and feed conversion ratio were measured weekly. At the end of the fifth week, six chickens per treatment were sacrificed to investigate the effects of NS and bentonite on carcass characteristics and hepatic histopathology. The results showed that providing broiler chickens’ diets with an adsorbent agent, such as NS or bentonite, can reduce the side effects of mycotoxins and enhance their growth performance. The best record was achieved with NS at 0.20%, compared with the control group and other dietary treatment groups. Accordingly, 0.20% of NS could be used in broiler chickens’ diets to minimize the harmful effects of mycotoxins.

scholarly journals Spirulina (Arthrospira fusiformis) as a potential protein source in practical diets for fry mariculture of Rufiji tilapia (Oreochromis urolepis urolepis)

2019 ◽  
Vol 18 (2) ◽  
pp. 57-67
Author(s):  
Deogratias P. Mulokozi ◽  
Matern S. Mtolera ◽  
Aviti J. Mmochi
Keyword(s):  
Growth Performance ◽  
Culture Media ◽  
Salt Water ◽  
Control Diet ◽  
Survival Rates ◽  
Daily Weight Gain ◽  
Feed Additive ◽  
Control Fish ◽  
Control Group ◽  
Feed Conversion

The effects on growth performance, feed utilization, survival and whole fish body proximate composition of replacing fishmeal (Rastrineobola argentea) with a locally available spirulina species (Arthrospira fusiformis) as a protein ingredient in the feed of Rufiji tilapia (Oreochromis urolepis urolepis) fry was examined. Fishmeal replacement with A. fusiformis was carried out at 5%, 15%, 25%, 35% and 100% (S5, S15, S25, S35 and S100), and the effect of the replacement was compared with the control diet (S0, 0% spirulina). Fish fry were stocked at an average initial weight of 0.57 g at 10 fish per 0.1 m3 and cultured for 60 days using full strength salt water (30-35 ppt). A. fusiformis was isolated from Momela Lake in Arusha National Park, Tanzania and propagated using selected culture media. It was observed that fish fry fed spirulina at a 5% fishmeal substitution level diet had better (p<0.05) final weight (8.48), average daily weight gain (0.132), specific growth rate (4.47), feed conversion rate (2.08) and protein efficiency ratio (1.37) compared to the control fish group. Growth performance in fish fed diet S15 was comparable with the control group (p>0.05). Spirulina supplementation had no effect on fish survival rates and meat quality. It appears that the Momella Lake spirulina may be an appropriate growth-stimulating plant protein when used as a feed additive in Rufiji tilapia mariculture. 

scholarly journals Effect of Probiotics on Growth Performance and Carcass Characteristics in Broilers at Farmer’s Door

2020 ◽  
pp. 1-5
Author(s):  
Hemant Kumar ◽  
Shikha Kumari
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Control Diet ◽  
Carcass Characteristics ◽  
Control Group ◽  
Feed Conversion ◽  
Commercial Diet ◽  
Group B

Aims: An experiment was conducted to investigate the comparative efficacy of two probiotics of different origins (yeast and bacterial based) on the growth performance and carcass characteristics of broilers chicken. Place and Duration of Study: The study was conducted at Krishi Vigyan Kendra, Purnea between January, 2020 to March, 2020. Methods: Two hundred and forty, day old chicks were kept and were randomly divided into 4 groups (A, B, C and D) and each group had 2 replicates of 20 chicks. Four (A, B, C and D) iso-caloric and iso-nitrogenous (ME 3000 kcal/ kg and CP 21.56%) broiler starter and finisher (ME 3000 kcal/ kg and CP 18.75%) diets were formulated containing Saccharomyces cerevisiae, Bacillus cereus toyoi and mixture of both @ 0.2% except group A which was control. Birds were raised for first week on commercial diet. Broiler starter and finisher diets were fed from 8-28 and 29-42 days, respectively. Results: A significant (P<0.05) effect was observed on overall performance of the birds fed diet containing probiotics. Probiotics in feed at 0.2% Saccharomyces cerevisiae supplementation improved body weight gain (+12.7%), feed intake (3.8%) and feed conversion efficiency (-8.7%) compared with the control diet. Among groups, no significant differences were observed in carcass traits but highest dressing percentage was recorded in group-B followed by group-C than group-D and lowest in control group. Conclusion: The result indicated that the addition of probiotics in feed containing 0.2% Saccharomyces cerevisiae improved broiler growth performances and it is beneficial to be used as supplement in feed of broiler chickens.

scholarly journals Growth Performance and Intestinal Morphometric Features of Broiler Chickens Fed on Dietary Inclusion of Yellow Mealworm (Tenebrio Molitor), Larvae Powder

2021 ◽  
Author(s):  
Shadi Sedgh-Gooya ◽  
Mehran Torki ◽  
Maryam Darbemamieh ◽  
Hassan Khamisabadi ◽  
Alireza Abdolmohamadi
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Control Diet ◽  
Tenebrio Molitor ◽  
Intestinal Morphology ◽  
Control Group ◽  
Feed Conversion ◽  
Negative Effects ◽  
Remarkable Effect

Abstract Climate change and the lack of conventional feed ingredients have made edible insects a highly nutritious alternative to feed production. The use of insects as food may help solve socio-economic and environmental problems around the world and be in line with the United Nations Sustainable Development Goals. In this study, the growth performance and intestinal morphology of broiler chickens were evaluated under the influence of adding different levels of Tenebrio molitor larvae meal (TM meal) to their diets. One hundred eighty day-old broiler chickens were divided into 3 treatments and 5 replications (12 chickens/pen). The experimental diets included a control diet and treatments containing 2.5 and 5% TM meal, which were fed to birds in the starter (0-10 days) and grower (11-25 days) stages, and during the final period (26-42 days), all birds were fed a regular finisher diet. The results showed that the diet containing TM meal had no remarkable effect on the mortality rate and feed intake (FI) of broilers (P > 0.05). In the period of starter, the addition of 2.5% TM meal to broilers diet increased body weight gain (BWG) than the control group (P £ 0.05). Also, the use of 2.5% TM meal in the starter period showed a significant effect on reducing the feed conversion ratio (FCR) compared to the birds fed by the control diet (P £ 0.05). Besides, the height of the villus, the depth of the crypt, and the ratio of them were not altered among the different treatments (P > 0.05). Overall, it can be concluded that TM meal could improve growth performance in the starter period, and had no negative effects on broilers' performance and intestinal morphology in all the periods of the experiment.

Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Flu Vaccine ◽  
Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

Combined administration of bacteriocin- -producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits

2013 ◽  
Vol 16 (4) ◽  
pp. 619-627 ◽  
Author(s):  
M. Pogány Simonová ◽  
A. Lauková ◽  
L. Chrastinová ◽  
I. Plachá ◽  
V. Strompfová ◽  
...  
Keyword(s):  
Growth Performance ◽  
Enterococcus Faecium ◽  
Average Daily Gain ◽  
Probiotic Strain ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Feed Conversion ◽  
Eleutherococcus Senticosus ◽  
Commercial Diet ◽  
And Control

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.

Effects of Moringa Oleifera Leaf Polysaccharide on Growth Performance and Immune Response of Broiler Chickens

2021 ◽  
Author(s):  
Hosameldeen Mohamed Husien ◽  
JunJie Huang ◽  
WeiLong Peng ◽  
ShuMei Zheng ◽  
JinGui Li
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Moringa Oleifera ◽  
The Body ◽  
Feed Consumption ◽  
Feed Additive ◽  
Control Group ◽  
High Dose ◽  
Tnf Α

Abstract Moringa oleifera (MO) is a widely used as the nutritious and non-traditional feed supplementation containing kinds of bioactive substances. However, the enhancement effect of Moringa oleifera leaf Polysaccharide (MOLP) as a feed additive in broilers growth performance and immunity remains unclear. In this study, MOLP was obtained by water extraction and alcohol precipitation method, then purified with Trichloroacetic acid (TCA) assay. Chickens were randomly divided into 4 groups, to receive different doses of MOLP (0, 0.1, 0.2, 0.4g/kg) in feed for 3 weeks. The body weight gain (BWG) and feed consumption were recorded for feed conversion ratio (FCR) and average daily feed intake (ADFI) calculation. Broiler chickens were sacrificed and sampled on day 14, 21, 28 (D 14, D 21, and D 28) respectively. Serological indicators, including total protein (TP), albumin (ALB), globulin (GLO), and creatinine (CREA) were detected. ELISA kits were applied for detecting the levels of immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-2 (IL-2), and tumor necrosis factor (TNF-α). From D 21 to D 28, the results showed that middle dose of MOLP significantly increased BWG and ADFI as well as liver and bursa indexes when compared with the control group. In addition, TP and GLO were also increased (P<0.05). All MOLP treatments enhanced the serum concentrations of IgG and IL-2 (P<0.01). Furthermore, results of quantitative RT-PCR showed that high dose of MOLP treatment significantly increased (P<0.001) the mRNA expression levels of IL-2 and TNF-α of chickens relative to the control group. In conclusion, the results showed that MOLP supplementation contributed to improve growth performance and immune response in broiler chickens, and MOLP could be considered as a promising feed additive.

scholarly journals Probiotic level effects on growth performance, carcass traits, blood parameters, cecal microbiota, and immune response of broilers

2016 ◽  
Vol 88 (2) ◽  
pp. 1011-1021 ◽  
Author(s):  
MOHAMMADREZA POURAKBARI ◽  
ALIREZA SEIDAVI ◽  
LEILA ASADPOUR ◽  
ANDRÉS MARTÍNEZ
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Control Diet ◽  
Carcass Traits ◽  
Blood Parameters ◽  
Feed Conversion ◽  
Probiotic Supplementation ◽  
Cecal Microbiota ◽  
Feed Conversion Rate

Probiotic effects on growth performance, carcass traits, blood parameters, cecal microbiota, and immune response of broilers were studied. Two hundred one-day-old male chickens were allocated to one of five treatments (four replicates of 10 birds per treatment): control, and the same control diet supplemented with 0.005%, 0.01%, 0.015% and 0.02% probiotics. Probiotics in feed at 0.01% or higher levels of supplementation improved body weight gain (+12%) and feed conversion rate (-5%) compared with the control. There were no effects on carcass traits, but the relative weights of drumsticks and wings showed increasing and decreasing linear responses, respectively, to probiotic supplementation level. Blood plasma glucose and albumin contents linearly increased (from 167.1 to 200.5 mg dl-1, and from 1.70 to 3.25 g dl-1) with increasing probiotic supplementation. Triglycerides and cholesterol contents were lower in probiotic supplemented treatments (average contents 71.3 and 125.3 mg dl-1 vs. 92.6 and 149.9 mg dl-1 in the control). Probiotics decreased cecal Escherichia coli counts, but had no effects on immunity related organs or immune response. The linear trends, either positive or negative, observed in many of the parameters studied, suggest that more studies are needed to establish the optimal concentration of probiotics in broiler feed.

scholarly journals Effects of Bacillus amyloliquefaciens LFB112 on Growth Performance, Carcass Traits, Immune, and Serum Biochemical Response in Broiler Chickens

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1427
Author(s):  
Marhaba Ahmat ◽  
Junhao Cheng ◽  
Zaheer Abbas ◽  
Qiang Cheng ◽  
Zhen Fan ◽  
...  
Keyword(s):  
Growth Performance ◽  
Bacillus Amyloliquefaciens ◽  
Broiler Chickens ◽  
Average Daily Gain ◽  
Carcass Traits ◽  
Serum Triglyceride ◽  
Basal Diet ◽  
Feed Conversion ◽  
Vegetative Cells ◽  
Serum Biochemical

This study aimed to investigate the effects of Bacillus amyloliquefaciens LFB112 on the growth performance, carcass traits, immune response, and serum biochemical parameters of broiler chickens. A total of 396 1 day old, mixed-sex commercial Ross 308 broilers with similar body weights were allotted into six treatment groups. The assigned groups were the CON group (basal diet with no supplement), AB (antibiotics) group (basal diet + 150 mg of aureomycin/kg), C+M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 powder with vegetative cells + metabolites), C group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 vegetative cell powder with removed metabolites), M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 metabolite powder with removed vegetative cells), and CICC group (basal diet + 5 × 108 CFU/kg Bacillus subtilis CICC 20179). Results indicated that chickens in the C+M, C, and M groups had higher body weight (BW) and average daily gain (ADG) (p < 0.05) and lower feed conversion ratio (FCR) (p = 0.02) compared to the CON group. The C+M group showed the lowest abdominal fat rate compared to those in the CON, AB, and CICC groups (p < 0.05). Compared to the CON group, serum IgA and IgG levels in the C+M, C, and M groups significantly increased while declining in the AB group (p < 0.05). B. amyloliquefaciens LFB112 supplementation significantly reduced the serum triglyceride, cholesterol, urea, and creatinine levels, while increasing the serum glucose and total protein (p < 0.05). In conclusion, B. amyloliquefaciens LFB112 significantly improved the growth performance, carcass traits, immunity, and blood chemical indices of broiler chickens and may be used as an efficient broiler feed supplement.

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