Effects of Moringa Oleifera Leaf Polysaccharide on Growth Performance and Immune Response of Broiler Chickens

2021 ◽  
Author(s):  
Hosameldeen Mohamed Husien ◽  
JunJie Huang ◽  
WeiLong Peng ◽  
ShuMei Zheng ◽  
JinGui Li
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Moringa Oleifera ◽  
The Body ◽  
Feed Consumption ◽  
Feed Additive ◽  
Control Group ◽  
High Dose ◽  
Tnf Α

Abstract Moringa oleifera (MO) is a widely used as the nutritious and non-traditional feed supplementation containing kinds of bioactive substances. However, the enhancement effect of Moringa oleifera leaf Polysaccharide (MOLP) as a feed additive in broilers growth performance and immunity remains unclear. In this study, MOLP was obtained by water extraction and alcohol precipitation method, then purified with Trichloroacetic acid (TCA) assay. Chickens were randomly divided into 4 groups, to receive different doses of MOLP (0, 0.1, 0.2, 0.4g/kg) in feed for 3 weeks. The body weight gain (BWG) and feed consumption were recorded for feed conversion ratio (FCR) and average daily feed intake (ADFI) calculation. Broiler chickens were sacrificed and sampled on day 14, 21, 28 (D 14, D 21, and D 28) respectively. Serological indicators, including total protein (TP), albumin (ALB), globulin (GLO), and creatinine (CREA) were detected. ELISA kits were applied for detecting the levels of immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-2 (IL-2), and tumor necrosis factor (TNF-α). From D 21 to D 28, the results showed that middle dose of MOLP significantly increased BWG and ADFI as well as liver and bursa indexes when compared with the control group. In addition, TP and GLO were also increased (P<0.05). All MOLP treatments enhanced the serum concentrations of IgG and IL-2 (P<0.01). Furthermore, results of quantitative RT-PCR showed that high dose of MOLP treatment significantly increased (P<0.001) the mRNA expression levels of IL-2 and TNF-α of chickens relative to the control group. In conclusion, the results showed that MOLP supplementation contributed to improve growth performance and immune response in broiler chickens, and MOLP could be considered as a promising feed additive.

Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Flu Vaccine ◽  
Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

scholarly journals Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens

2021 ◽  
Vol 8 ◽  
Author(s):  
Teketay Wassie ◽  
Zhuang Lu ◽  
Xinyi Duan ◽  
Chunyan Xie ◽  
Kefyalew Gebeyew ◽  
...  
Keyword(s):  
Immune Response ◽  
Gut Microbiota ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Marine Algae ◽  
Basal Diet ◽  
Control Group ◽  
Villus Height ◽  
Crypt Depth ◽  
Caecal Microbiota

Marine algae polysaccharides have been shown to regulate various biological activities, such as immune modulation, antioxidant, antidiabetic, and hypolipidemic. However, litter is known about the interaction of these polysaccharides with the gut microbiota. This study aimed to evaluate the effects of marine algae Enteromorpha (Ulva) prolifera polysaccharide (EP) supplementation on growth performance, immune response, and caecal microbiota of broiler chickens. A total of 200 1-day-old Ross-308 broiler chickens were randomly divided into two treatment groups with ten replications of ten chickens in each replication. The dietary treatments consisted of the control group (fed basal diet), and EP group (received diet supplemented with 400 mg EP/kg diet). Results showed that chickens fed EP exhibited significantly higher (P &lt; 0.05) body weight and average daily gain than the chicken-fed basal diet. In addition, significantly longer villus height, shorter crypt depth, and higher villus height to crypt depth ratio were observed in the jejunal and ileal tissues of chickens fed EP. EP supplementation upregulated the mRNA expression of NF-κB, TLR4, MyD88, IL-2, IFN-α, and IL-1β in the ileal and jejunal tissues (P &lt; 0.05). Besides, we observed significantly higher (P &lt; 0.05) short-chain volatile fatty acids (SCFAs) levels in the caecal contents of the EP group than in the control group. Furthermore, 16S-rRNA analysis revealed that EP supplementation altered gut microbiota and caused an abundance shift at the phylum and genus level in broiler chicken. Interestingly, we observed an association between microbiota and SCFAs production. Overall, this study demonstrated that supplementation of diet with EP promotes growth performance, improves intestinal immune response and integrity, and modulates the caecal microbiota of broiler chickens. This study highlighted the application of marine algae polysaccharides as an antibiotic alternative for chickens. Furthermore, it provides insight to develop marine algae polysaccharide-based functional food and therapeutic agent.

Influence of feed additive with Betulin on metabolism and productivity of broiler chickens

2021 ◽  
pp. 3-18
Author(s):  
S. A. Shpуnova ◽  
O. A. Yadrishchenskaya ◽  
T. V. Selina ◽  
E. A. Basova
Keyword(s):  
Broiler Chickens ◽  
Practical Importance ◽  
Live Weight ◽  
Biologically Active ◽  
Feed Consumption ◽  
Feed Additive ◽  
Control Group ◽  
Meat Production ◽  
Birch Bark ◽  
Experimental Group

In order to increase the meat productivity of broiler chickens, increase production volumes and reduce the prime-cost of poultry products, it is necessary not only to ensure balanced feeding and compliance with production technology but also to include in the poultry diet non-traditional additives, biologically active substances that contribute to the normalization of metabolic processes and improve the digestibility of feed nutrients. One of these dietary additives is Betulin extracted from birch bark, which has antioxidant and immunomodulatory properties. In this regard, the purpose of the research was to study the influence of compound feed with the introduction of 2 % feed additive based on a synthetic microspherical nanoporous carbon carrier containing biologically active Betulin in its composition on the productivity of broilers. The results of studies on broiler chickens using compound feed with 2 % feed additive have been presented in the article. It has been found that the feed consumption per 1 kg of live weight gain in poultry of the experimental group have been lower by 9,5 % due to better absorption of nutrients, the live weight of broiler chickens of the experimental group at the age of 42 days was 2625,6 g and it was higher than the control group by 3,68 %. The digestibility of crude protein and fat reached 92,73 and 88,03 %, which exceeded the control group by 1,03 and 3,03 abs.%, respectively. It has been found that the use of compound feed with 2 % of the feed additive with the biologically active substance is of practical importance in connection with the increase in the productivity of broilers and the level of profitability of meat production by 8,2 abs.%.

Pengaruh penggunaan ramuan herbal sebagai feed additive terhadap in come over feed cost ayam broiler

2017 ◽  
Vol 5 (2) ◽  
pp. 100-105
Author(s):  
Ferdianus Nono ◽  
Dyah Lestari Yulianti ◽  
Aju Tjatur Nugroho Krisnaningsih
Keyword(s):  
Drinking Water ◽  
Broiler Chickens ◽  
Feed Consumption ◽  
Feed Additive ◽  
Control Group ◽  
Feed Cost ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Feed Costs ◽  
The Cost

  ABSTRAK Tujuan penelitian ini adalah untuk mengetahui pengaruh dari penggunaan ramuan herbal terhadap income over feed cost  ayam broiler. Materi  yang digunakan pada  penelitian ini adalah: ayam broiler DOC. Penelitian ini dirancang dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dan masing-masing perlakuan terdiri dari 4 ulangan. Perlakuan yang digunakan dalam penelitian ini adalah penggunaan ramuan herbal dalam air minum dengan level penggunaan sebagai berikut : P0: tanpa penambahan ramuan herbal, P1:air + 10 % ramuan herbal, P2: air  + 15 % ramuan herbal, P3: air + 20% ramuan herbal. Variabel yang di amati  dalam penelitian ini adalah konsumsi pakan, biaya pakan, dan income over feed cost ayam broiler. Data yang diperoleh (income over feed cost) di analisis dengan menggunakan analisis ragam dengan rancangan Acak Lengkap (RAL). Hasil penelitian menunjukan bahwa biaya pakan tertinggi terdapat pada kelompok perlakuan P3 diikuti berturut-turut oleh P1, P2, P0 dan yang terendah adalah P0. Sedangkan pada P0 biaya pakan paling rendah karena tidak ada penggunaan ramuan herbal. Berdasarkan data penerimaan, penerimaan tertinggi terdapat pada kelompok kontrol P1 Rp 31.430,6  sedangkan penerimaan terandah terdapat pada kelompok P0 sebesar Rp 24.814,8.100 Rendahnya penerimaan dalam penelitian ini disebabkan oleh rendahnya bobot badan ayam broiler selama penelitian. Income Over Feed Cost selama penelitian yang tertinggi terdapat pada kelompok P3 (3.568), dan terendah terdapat pada perlakuan  P1 (979) P0 (1.964), P2 (1.937) karena biaya pakan lebih tinggi dari pada penerimaan.Ini berarti bahwa penggunaan ramuan herbal  dalam air minum berpengaruh sangat nyata (P< 0,01) terhadap Income Over Feed Cost. Berdasarkan hasil penelitian dapat disimpulkan bahwa penggunaan ramuan herbal sebagai feed additive  memberikan pengaruh sangat nyata terhadap IOFC ayam broiler.   Abstract The purpose of this study was to determine the effect of using herbal ingredients on income over feed costs of broiler chickens. The material used in this study was: broiler DOC. This study was designed using a Completely Randomized Design (CRD) consisting of 4 treatments and each treatment consisted of 4 replications. The treatment used in this study is the use of herbal ingredients in drinking water with the use level as follows: P0: without the addition of herbal ingredients, P1: water + 10% herbal ingredients, P2: water + 15% herbal ingredients, P3: water + 20% herbal concoctions. The variables observed in this study were feed consumption, feed costs, and income over feed costs for broiler chickens. The data obtained (income over feed cost) is analyzed using a variety of analysis with a completely randomized design (CRD). The results showed that the highest feed costs were found in the P3 treatment group followed by P1, P2, P0 and the lowest was P0. Whereas at P0 the cost of feed is lowest because there is no use of herbal ingredients. Based on acceptance data, the highest revenue was in the P1 control group of Rp. 31,430.6 while the lowest acceptance was in the P0 group of Rp. 24,814.8,100. The low acceptance in this study was caused by the low body weight of broiler chickens during the study. Income Over Feed Cost during the highest research was found in the P3 group (3,568), and the lowest was found in the treatment of P1 (979) P0 (1,964), P2 (1,937) because the cost of feed was higher than acceptance. drinking water has a very significant effect (P <0.01) on Income Over Feed Cost. Based on the results of the study it can be concluded that the use of herbal ingredients as additive feeds has a very significant effect on IOFC broiler chickens.

The effect of stevia as a feed sweetener on weight gain and feed consumption of broiler chickens

1996 ◽  
Vol 76 (2) ◽  
pp. 267-269 ◽  
Author(s):  
D. J. Wood ◽  
A. Lirette ◽  
D. C. Crober ◽  
H. Y. Ju
Keyword(s):  
Weight Gain ◽  
Experimental Design ◽  
Growth Performance ◽  
Key Words ◽  
Broiler Chickens ◽  
Feed Consumption ◽  
Feed Additive ◽  
Male Chicken

One thousand day-old male chicken broilers housed in 16 floor pens to 42 d of age were used to study the effect of stevia on weight gain and feed consumption. The experimental design was a randomized complete block consisting of four diets (0%, 0.0085%, 0.0425% and 0.085% stevia) each replicated four times. Stevia did not appear to improve feed consumption or weight gain of broilers when used as a feed additive at the concentrations tested in this study. Key words: Broilers, stevia, feed additive, growth performance

scholarly journals Features of protein metabolism in the body of broiler chickens when using the feed additive Peak-Antistress in the diet

2020 ◽  
pp. 103-110
Author(s):  
A. V. Mitfakhutdinov ◽  
E. R. Saifulmuliukov
Keyword(s):  
Broiler Chickens ◽  
Protein Composition ◽  
Feed Additives ◽  
The Body ◽  
Feed Additive ◽  
Control Group ◽  
Protein Accumulation ◽  
White Meat ◽  
Scientific Publications ◽  
Experimental Group

Scientific publications describe the positive effect of feed additives and pharmacological complexes on metabolic processes in the body of broiler chickens, in particular, on the protein composition of the blood, meat productivity and protein accumulation in meat. The Peak-Antistress feed additive developed at the Department of Morphology, Physiology and Pharmacology of the South Ural State Agrarian University allows to reduce the technological load on the poultry body by stimulating the general metabolism and complex antioxidant action. Experiments on the use of the feed additive Peak-Antistress were carried out on broiler chickens of the final hybrid of the Arbor Acres cross in an industrial-type poultry farm with floor technology. The poultry was divided into three groups of 6,000 chickens each and kept in one workshop in separate sections. The control group received the main diet, the 1st experimental group received the main diet and feed additive at a dose of 1269 g / t of feed 5 days before slaughter, the 2nd experimental group received the main diet and feed additive at a dose of 1693 g / t of feed 5 days before slaughter. Slaughter of broiler chickens was carried out according to the technological instructions of the enterprise on the 38th day. Against the background of the use of the feed additive in the experimental groups of broiler chickens, stimulation of protein metabolism was observed, as evidenced by an increase in total protein in the blood by 7.9–20.1%, an increase in the level of protein in white meat by 0.1–0.3%, and a tendency to an increase in amino acids in white meat protein by 0.2–12.3%.

scholarly journals Preliminary Study on the Effect of Bacillus amyloliquefaciens TL on Cecal Bacterial Community Structure of Broiler Chickens

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Yuxuan Hong ◽  
Yingxian Cheng ◽  
Yanjuan Li ◽  
Xiaowen Li ◽  
Zutao Zhou ◽  
...  
Keyword(s):  
Growth Performance ◽  
Bacillus Amyloliquefaciens ◽  
Broiler Chickens ◽  
High Throughput Sequencing ◽  
Control Diet ◽  
Probiotic Strain ◽  
Feed Additive ◽  
Control Group ◽  
Feed Conversion ◽  
Cecal Microbiota

Probiotics can promote the health and growth performance of animals through modulation of intestinal microbiota. When used as a feed additive, they have the potential to minimize or abolish the use of antibiotics. In this study, we investigated the effect of the probiotic strain Bacillus amyloliquefaciens TL on the growth performance and cecum microflora composition in Cobb 500 broiler chickens. In total, 180 broilers were randomly divided into three groups—each group comprised 4 pens, and each pen contained 15 chickens. The three groups were fed either a control diet, or a diet supplemented with either the antibiotic chlortetracycline or B. amyloliquefaciens TL. Broilers were weighed, and cecum contents were collected on days 7, 14, 21, and 35, respectively. The broilers in both the antibiotic and probiotic groups exhibited significant weight gain compared with controls, exhibiting increases of 16.02% and 13.40%, respectively, after 35 days (P<0.01). Similarly, the feed conversion ratio (FCR, 1–35 days) of broilers in the chlortetracycline and B. amyloliquefaciens TL groups was lower than that of the controls. HiSeq high-throughput sequencing of 16S rRNA of the cecal microbiota was performed on days 7, 14, 21, and 35, respectively. The Firmicutes/Bacteroidetes ratio was higher in the chlortetracycline and B. amyloliquefaciens TL groups than in the control group on days 14, 21, and 35, and especially on day 21. The prevalence of genera Oscillospira, Ruminococcus, Butyricicoccus, and Faecalibacterium (Firmicutes) was higher in the antibiotic and probiotic groups, while that of Bacteroides, Parabacteroides (Bacteroidetes), and Lactobacillus was higher in the control group. In this study, the changes in the microbiota of the probiotic group were similar to those in the antibiotic group. These results suggest that the probiotic strain B. amyloliquefaciens TL can modulate the cecal microbiota of broilers similar to chlortetracycline.

The effectiveness of feeding complex mineral and vitamin feed additive «Nadezhda» to lactating cows

2021 ◽  
pp. 30-37
Author(s):  
A. R. Farkhutdinova ◽  
M. T. Sabitov ◽  
M. G. Malikova
Keyword(s):  
The Body ◽  
Metabolic Energy ◽  
Feed Consumption ◽  
Feed Additive ◽  
Control Group ◽  
Black And White ◽  
Lactating Cows ◽  
White Breed ◽  
Quality Of Milk ◽  
Experimental Group

The feasibility of using the complex mineral and vitamin feed additive “Nadezhda” in the rations of lactating first-calf heifers of Black-and-White breed has been examined in the article. It has been established that feed contain a low content of elements such as calcium, phosphorus, magnesium, sulfur, manganese, copper, zinc and cobalt. The ratio of nutrients and minerals, the degree of their availability for assimilation by the body does not provide their need. The use of the complex mineral and vitamin feed additive contributed to an increase in the milk productivity of first-calf heifers and the quality of milk, feed consumption per unit of production. All tested recipes of the complex mineral and vitamin feed additive, which have different versions of macro-, microelements and vitamins in their composition had a positive effect on the metabolic processes in the body of animals. For the production of 1 kg of milk in the experimental groups of cows less metabolic energy was spent by 5,09 (P < 0,05) and 8,06 % (P < 0,05), respectively, raw protein by 5,19 and 8,19 % (P < 0,05), digestible protein by 5,12 (P < 0,05) and 8,15 % (P < 0,05) compared with the animals of the control group. At the same time from the cows of the 1st experimental group have been received more milk by 151,6 kg in the amount of 2489 rubles, from the 2nd experimental group by 250,6 kg of milk in the amount of 4248,0 rubles. The prime cost of production of 1 kg of milk in cows of the 1st experimental group in comparison with the animals of the control group decreased by 0,72 rubles, and the 2nd experimental group by 1,21 rubles. The level of profitability of milk production in cows have been fed with additive recipe No. 1, in comparison with the animals of the control group increased by 3,71 abs.%, recipe No. 2 by 9,91 abs.%.

scholarly journals Influence of stocking density on body conformation in broilers

2007 ◽  
Vol 61 (5-6) ◽  
pp. 313-323
Author(s):  
Almira Softic ◽  
Abdulah Gagic ◽  
Aida Kavazovic ◽  
Cazim Crnkic ◽  
Velija Katica ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Stocking Density ◽  
Live Weight ◽  
The Body ◽  
Feed Consumption ◽  
Control Group ◽  
Body Conformation ◽  
Live Body Weight ◽  
Research Findings ◽  
The One

In this experiment the influence of stocking density on the body conformation of broiler carcasses was investigated. One hundred and twenty broiler chickens were divided into three groups; each group comprised 40 chickens. At the end of the first week of the fattening period 20 chickens in each group were marked by random sampling. The first testing group (P1) represented the one with a lower stocking density (12 chickens per square meter); the second one (P2) with a higher stocking density (18 chickens per square meter), while the control group (K) was set in accordance with the technological recommendations (15 chickens per square meter). Breast circumference, drumstick circumference, keel length (crista sterni), breast depth and breast angle were monitored and measured on a weekly basis. Body conformation measures were determined on the carcasses in a horizontal position with their backs placed on the table. The appropriate instruments such as millimetre tape, caliper and ZP-3 protractor were used. In addition, a weekly live weight and feed consumption were measured. Production results (live weight, gain, feed-to-gain ratio and European Production Index (EPI) were calculated. Carcass grades were calculated based on the mass ratio of the cooled carcass and the live body weight before slaughtering. The research findings have confirmed that overcrowding in production facilities is always risky in regard to the expected production results. In contrast, by fully conforming to the production technology requirements, it is possible to achieve better production results.

scholarly journals The growth performance and immunity of broiler chickens affected by dietary chitosan oligosaccharides

2020 ◽  
Author(s):  
Hang Zhao ◽  
Saikun Pan
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Average Daily Gain ◽  
Control Group ◽  
High Dose ◽  
Feed Conversion ◽  
Chitosan Oligosaccharides ◽  
Feeding Trials ◽  
Factor Α ◽  
Necrosis Factor

Abstract Background: The present study aimed to explore the effects of dietary chitosan oligosaccharides (COs) on the growth performance and immunity of broiler chickens.Methods: Four test diets were supplemented with different doses of COs (0, 50, 100 and 150 mg/kg) and formulated. Triplicate groups of broiler chickens were fed with one of the diets ad libitum for 6 weeks. Results: After the end of the feeding trials, oral CO administration improved average daily gain; the activities of digestive amylase, lipase and protease; the activities of serum superoxide dismutase and glutathione peroxidase; serum tumor necrosis factor-α, interleukin (IL)-1, IL-2, IgG, IgM and IgA levels; and reduced feed conversion ratio and malondialdehyde level compared with those of the control group. However, a high dose of CO (150 g/kg) did not further increase its efficiency compared with the moderate dose of CO (100 g/kg). Conclusions: Oral CO administration improved the growth performance and immunity of juvenile broiler chickens and could be used as a feed supplement.

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