scholarly journals Diagnostic Value of Interferon-Gamma Release Assays Combined with Multiple Indicators for Tuberculous Peritonitis

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Xidong He ◽  
Yuanxue Gao ◽  
Qi Liu ◽  
Zhifang Zhao ◽  
Wanhang Deng ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Disease Control ◽  
Interferon Gamma ◽  
Diagnostic Value ◽  
Tuberculous Peritonitis ◽  
The Other ◽  
Control Group ◽  
Healthy Individuals ◽  
Multiple Indicators ◽  
Interferon Gamma Release Assays

Objective. To investigate the diagnostic value of interferon-gamma release assays combined with multiple indicators for tuberculous peritonitis. Methods. Patients who were admitted to the hospital due to suspected tuberculous peritonitis were prospectively included during the 30-month study period. Moreover, healthy individuals were recruited and included in the control group. All the study participants were assessed using various indexes, such as interferon-gamma release assays. Results. A total of 180 patients with suspected tuberculous peritonitis were enrolled, and 24 were excluded. 73 patients with a confirmed diagnosis of tuberculous peritonitis were included in the tuberculous peritonitis group, 83 patients with other diseases in the other-disease control group, and 52 healthy individuals in the control group. Moreover, 83 patients in the other-disease control group and 52 participants in the control group were identified as 135 nontuberculous peritonitis patients. The area under the receiver operating characteristics curve for the QuantiFERON-TB test was 0.851 (95% confidence interval: 0.799–0.903), and the optimal cutoff value was 0.55 IU/mL, which corresponds to a sensitivity and specificity of 86.30% and 80.00%, respectively. The receiver operating characteristic curves for the combination of the QuantiFERON-TB test and the use of erythrocyte sedimentation rate, serum adenosine deaminase level, serum cancer antigen 125 level, and hypersensitive C-reactive protein level had an area under the curve of 0.859 (95% confidence interval: 0.809–0.909), with a sensitivity and specificity of 97.26% and 62.96%, respectively. Conclusions. The combined use of the QuantiFERON-TB test and multiple indexes can significantly improve the accuracy of diagnosing tuberculous peritonitis.

scholarly journals Serum Protein Profile Of Iraqi Hydatidosis Patients with Different Sites of Infection

2007 ◽  
Vol 4 (4) ◽  
pp. 612-616
Author(s):  
Baghdad Science Journal
Keyword(s):  
Serum Protein ◽  
Protein Profile ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Healthy Individuals ◽  
Banding Patterns ◽  
Blood Samples ◽  
Molecular Weights ◽  
Serum Protein Profile

Blood samples of One hundred and twenty patients from different hospitals in Baghdad infected with hydatidosis in different sites of the body (Liver, Lung, multiorgans and kidney) were collected for this study. On the other hand, 30 healthy individuals were included as a control group. This study was conducted to evaluate the effect of this disease on the serum protein profile of the patients using electrophoresis. The results revealed four different protein banding patterns with difference in number of bands and their molecular weights in comparison to the control group, and these differences depended on the site of infection. However the data showed a presence of the same band in all patients with different site of infection.

The Diagnostic Value of Whole Blood lncRNA ENST00000550337.1 for Pre-Diabetes and Type 2 Diabetes Mellitus

2017 ◽  
Vol 125 (06) ◽  
pp. 377-383 ◽  
Author(s):  
Xuejie Li ◽  
Zhenzhou Zhao ◽  
Chuanyu Gao ◽  
Lixin Rao ◽  
Peiyuan Hao ◽  
...  
Keyword(s):  
Noncoding Rna ◽  
Diagnostic Value ◽  
Differentially Expressed ◽  
Control Group ◽  
Healthy Individuals ◽  
Diabetes Group ◽  
The Third ◽  
Diagnostic Capacity ◽  
Independent Cohort

Abstract This study aims to investigate long noncoding RNA (lncRNA) as biomarker for pre-diabetes and T2DM. LncRNAs in the peripheral blood of 6 healthy individuals and 6 T2DM patients were collected for microarray analysis. Then 5 candidate biomarkers from the differentially expressed lncRNAs were chosen and verified in a larger independent cohort (control group=20; pre-diabetes group=20; and T2DM group=20). The diagnostic capacity of ENST00000550337.1 was further tested in the third cohort (control group, n=60; pre-diabetes group, n=63; and T2DM group, n=64). A total of 17 lncRNAs were found to be differentially expressed between the 2 groups. 14 lncRNAs of these were upregulated in T2DM patients and 3 were downregulated. 5 upregulated lncRNAs were selected as potential biomarkers and verified in the second cohort, and the expression levels of 3 lncRNAs increased gradually from the control group to the pre-diabetes group to the T2DM group. The diagnostic value of ENST00000550337.1 was then tested in the third cohort, and its high diagnostic value for pre-diabetes and T2DM was confirmed. LncRNA ENST00000550337.1 is a potential diagnostic biomarker for pre-diabetes and T2DM.

scholarly journals Evaluation of Salivary Interferon-Gamma Level in Patients with Recurrent Aphthous Stomatitis

2020 ◽  
Author(s):  
Marieh Honarmand ◽  
Ramin Saravani ◽  
Hossein Ansari ◽  
Iman Teimoori
Keyword(s):  
Interferon Gamma ◽  
Systemic Disease ◽  
Recovery Period ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Control Group ◽  
Healthy Individuals ◽  
Salivary Level ◽  
The Mean ◽  
Control Study

Introduction: Recurrent aphthous stomatitis (RAS) is defined by recurring ulcers restricted to oral mucosa in the patients with no other signs of systemic disease. The purpose of this study was to evaluate the salivary level of interferon gamma in patients with RAS. Methods: In this case-control study, 30 patients with RAS (in Recurrence and recovery period) in the experimental and 25 healthy people as the control group were compared. Salivary interferon gamma level was evaluated by the use of ELISA method. Data were analyzed via SPSS version 16 software with Mann-U-Whitney test and Wilcoxon. P <0.05 was considered significant. Results: The mean salivary interferon gamma levels were 45.01±31.02 pg/ml and 32.04±19.02 pg/ml in the patients with RAS and healthy individuals, respectively. (P>0.001). In addition, interferon gamma level was 45.01± 31.02 pg/ml and 58.03± 52.04 pg/ml in the recurrence and recovery periods, respectively. (P=0.67). Conclusion: Salivary interferon gamma levels were higher in the patients with RAS than healthy individuals.

scholarly journals Cytokine Gene Polymorphisms in Autoimmune Thyroid Disease

2000 ◽  
Vol 85 (5) ◽  
pp. 1984-1988 ◽  
Author(s):  
P. J. Hunt ◽  
S. E. Marshall ◽  
A. P. Weetman ◽  
J. I. Bell ◽  
J. A. H. Wass ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Thyroid Disease ◽  
Odds Ratio ◽  
Autoimmune Thyroid Disease ◽  
Transforming Growth Factor ◽  
Inflammatory Responses ◽  
The Other ◽  
Control Group ◽  
Potential Candidate ◽  
Autoimmune Thyroid

Abstract Susceptibility to the autoimmune thyroid diseases, Graves’ disease (GD) and autoimmune hypothyroidism (AIH), depends on a complex interaction between environmental and genetic factors. The human leukocyte antigen and cytotoxic T lymphocyte-associated-4 regions appear to influence susceptibility to disease, but the effect is not major, and the other genes remain unknown. Cytokines are crucial in the regulation of immune and inflammatory responses and therefore are potential candidate genes for autoimmune thyroid disease. In a case-control study, using a unified method of genotyping, we have examined 15 polymorphisms in 9 cytokine genes in 215 patients with autoimmune thyroid disease (GD, 138; AIH, 77) and 101 normal controls. Polymorphisms in the genes for interleukin-1α (IL-1α), IL-1β, IL-1 receptor antagonist, IL-1 receptor 1, IL-4, IL-4 receptor, IL-6, IL-10, and transforming growth factor-β were investigated. Genotyping was performed using the PCR and sequence-specific primers. Analysis showed a reduced frequency of the variant t allele in the IL-4 promoter polymorphism (position −590) in patients with GD and in the entire patient group (GD and AIH) compared with the control group [corrected P (Pc) = 0.00004 and Pc &lt; 0.00001 for GD and all patients, respectively]. This was reflected in a reduction in the heterozygote genotype in the patient groups compared to the controls [c/t heterozygotes GD, 12%; Pc = 0.06, odds ratio, 0.4 (95% confidence interval, 0.2–0.7); all patients, 11%; Pc = 0.008; odds ratio, 0.4 (95% confidence interval, 0.2–0.7); control subjects, 23%]. There were no significant differences between the study groups for the other polymorphisms examined, and subgroup analysis revealed no association with clinical parameters of disease. These results suggest that an IL-4 variant or a closely linked gene has a modest protective effect against the development of autoimmune thyroid disease, particularly GD. This variation in the IL-4 gene may provide further clues to the pathogenesis of autoimmune thyroid disease and other organ-specific autoimmune diseases. Furthermore, these results suggest that subtle variation in immunoregulatory genes may be associated with autoimmune disease states.

Diagnostic Value of Interferon- gamma Release Assays (QuantiFERON-TB Gold® In Tube) in Childhood Tuberculosis

2011 ◽  
Vol 79 (2) ◽  
pp. 183-187 ◽  
Author(s):  
Rajeshwar Dayal ◽  
Vikas Verma ◽  
B. Sharma ◽  
G. Kumar ◽  
N. Kumar ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Diagnostic Value ◽  
Childhood Tuberculosis ◽  
Interferon Gamma Release Assays

scholarly journals Pulmonary Immune-Compartment-Specific Interferon Gamma Responses in HIV-Infected Individuals with Active Tuberculosis (TB) in an Area of High TB Prevalence

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
S. Buldeo ◽  
D. M. Murdoch ◽  
M. S. Suchard
Keyword(s):  
Interferon Gamma ◽  
Peripheral Blood ◽  
Intracellular Cytokine Staining ◽  
Control Group ◽  
Purified Protein Derivative ◽  
Tb Control ◽  
Diagnostic Potential ◽  
Interferon Gamma Release Assays ◽  
Secretory Antigen ◽  
Smear Negative

There is a paucity of data on the pulmonary immune-compartment interferon gamma (IFNγ) response toM. tuberculosis, particularly in settings of high tuberculosis (TB) prevalence and in HIV-coinfected individuals. This data is necessary to understand the diagnostic potential of commercially available interferon gamma release assays (IGRAs) in both the pulmonary immune-compartment and peripheral blood. We used intracellular cytokine staining by flow cytometry to assess the IFNγ response to purified protein derivative (PPD) and early secretory antigen 6 (ESAT6) in induced sputa (ISp) and blood samples from HIV-infected, smear-negative, TB suspects. We found that individuals with active TB disease produced significantly less IFNγ in response to PPD in their induced sputa samples than individuals with non-active TB (control group). This difference was not reflected in the peripheral blood, even within the CD27− CD4+ memory T lymphocyte population. These findings suggest that progression to active TB disease may be associated with the loss of IFNγ secretion at the site of primary infection. Our findings highlight the importance of studying pulmonary immune-compartmentM. tuberculosisspecific responses to elucidate IFNγ secretion across the spectrum of TB disease.

scholarly journals The urinary biopterin in autism spectrum disorder

Pteridines ◽  
2021 ◽  
Vol 32 (1) ◽  
pp. 17-22
Author(s):  
Aleksandra Waligóra ◽  
Aleksandra Damasiewicz-Bodzek ◽  
Piotr Gorczyca ◽  
Sławomir Waligóra ◽  
Krystyna Tyrpień-Golder
Keyword(s):  
Autism Spectrum Disorder ◽  
Autism Spectrum ◽  
Spectrum Disorder ◽  
The Other ◽  
Urine Samples ◽  
Control Group ◽  
Healthy Children ◽  
Healthy Individuals ◽  
Children With Asd ◽  
Significant Difference

Abstract Objective The aim of the study was to determine whether biopterin is present in significantly lower quantities in urine samples of patients with autism spectrum disorder (ASD) compared to healthy individuals. Methods The concentration of biopterin in urine samples was measured by ELISA using commercially available kit. The study involved 53 children aged 3–16 years with ASD and 60 healthy children aged 2–14 years. Results Significantly lower biopterin concentration was observed in autistic patients compared to the control group. However, no significant difference was observed between mild, moderate, and severe ASD. Conclusion One of the potential causes of decrease in urinary biopterin levels may be tetrahydrobiopterin (BH4) deficiency, which has extensive and serious health consequences for the nervous system. The results of measuring biopterin as a fully oxidized form of BH4 may suggest that biosynthesis or regeneration of BH4 may be decreased in children with ASD. On the other hand, decreased urinary biopterin levels in children with ASD may be due to BH4 overuse, a good regeneration process, and decreased urinary excretion; and abnormalities in BH4 metabolism appear to be related to the aetiology of ASD or may be due to ASD.

Relationships between values of myocardial remodeling parameters and level of apoptosis biomarkers in patients with ischemic cardiomyopathy

2021 ◽  
Vol 1 (30) ◽  
pp. 38-42
Author(s):  
G. N. Mukhambetova ◽  
O. S. Polunina ◽  
L. P. Voronina ◽  
N. B. Greenberg ◽  
E. A. Polunina ◽  
...  
Keyword(s):  
Left Ventricle ◽  
Ischemic Cardiomyopathy ◽  
The Other ◽  
Control Group ◽  
Annexin A5 ◽  
Myocardial Remodeling ◽  
Healthy Individuals ◽  
Systolic Volume ◽  
Sphericity Index ◽  
Close Relationship

The aim of the research. To study levels of the apoptosis biomarkers annexin A5 (AnxA5) and Bcl‑2 and to identify the presence of correlations of structural and functional parameters of the myocardium with the level of the studied biomarkers in patients with ischemic cardiomyopathy (ICMP).Materials and methods. Patients with ICMP (n = 47) were examined as the main group. The control group included 30 somatically healthy individuals. Bcl‑2 and APA5 levels were determined in the blood serum by the enzyme immunoassay.Results. It was found that in the group of patients with ICMP. The level of AnxA5 was statistically significantly higher (p < 0.001), and the level of Bcl‑2 was statistically significantly lower (p < 0.001) than in the control group. Based on the results of the correlation analysis a noticeable close relationship on the Cheddock scale was revealed between levels of the studied apoptosis biomarkers and the values of the sphericity index of in diastole and systole of left ventricle (LV), final diastolic and systolic volume of LV and ejection fraction of LV. Between the other parameters of myocardial remodeling and levels of the studied biomarkers of apoptosis, the closeness of relationships on the Cheddock scale were weak and moderate.

scholarly journals Rate of tuberculosis infection in children and adolescents with household contact with adults with active pulmonary tuberculosis as assessed by tuberculin skin test and interferon-gamma release assays

2015 ◽  
Vol 144 (4) ◽  
pp. 712-723 ◽  
Author(s):  
M. A. G. FERRARINI ◽  
F. G. SPINA ◽  
L. Y. WECKX ◽  
H. M. LEDERMAN ◽  
M. I. De MORAES-PINTO
Keyword(s):  
Children And Adolescents ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Interferon Gamma ◽  
Exposed Group ◽  
Tuberculosis Infection ◽  
Control Group ◽  
Control Groups ◽  
Interferon Gamma Release Assays ◽  
And Control

SUMMARYTuberculosis (TB) infection was evaluated in Brazilian immunocompetent children and adolescents exposed and unexposed (control group) to adults with active pulmonary TB. Both groups were analysed by clinical and radiological assessment, TST, QFT-IT and T-SPOT.TB. The three tests were repeated after 8 weeks in the TB-exposed group if results were initially negative. Individuals with latent tuberculosis infection (LTBI) were treated and tests were repeated after treatment. Fifty-nine TB-exposed and 42 controls were evaluated. Rate of infection was 69·5% and 9·5% for the exposed and control groups, respectively. The exposed group infection rate was 61% assessed by TST, 57·6% by T-SPOT.TB, and 59·3%, by QFT-IT. No active TB was diagnosed. Agreement between the three tests was 83·1% and 92·8% in the exposed and control groups, respectively. In the exposed group, T-SPOT.TB added four TB diagnoses [16%, 95% confidence interval (CI) 1·6–30·4] and QFT-IT added three TB diagnoses (12%, 95% CI 0–24·7) in 25 individuals with negative tuberculin skin test (TST). Risk factors associated to TB infection were contact with an adult with active TB [0–60 days: odds ratio (OR) 6·9; >60 days: OR 27·0] and sleeping in the same room as an adult with active TB (OR 5·2). In Brazilian immunocompetent children and adolescents, TST had a similar performance to interferon-gamma release assays and detected a high rate of LTBI.

Increased Levels of Albumin in Bronchial Washing Fluid of Patients with Bronchial Carcinoma. Could Albumin be Considered as a Tumor Marker?

2004 ◽  
Vol 19 (4) ◽  
pp. 316-321 ◽  
Author(s):  
N. Charokopos ◽  
M. Leotsinidis ◽  
M. Tsiamita ◽  
H.P. Kalofonos ◽  
P. Vasillakos ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Tumor Markers ◽  
Bronchial Carcinoma ◽  
Diagnostic Value ◽  
The Other ◽  
Control Group ◽  
Cross Sectional ◽  
Significant Difference ◽  
Bronchial Washing

Background The aim of this study was to evaluate the significance of albumin in bronchial washing fluid (BWF) and its relationship to three tumor markers (CEA, CA 19–9 and NSE). Methods Serum and BWF samples were collected in a group of 60 patients. Albumin and tumor markers in the BWF and serum of three groups: a control group (CG), a chronic bronchitis group (CBG) and a lung cancer group (CaG), were analyzed in a prospective cross-sectional study. The diagnostic yields of the tests in each environment (serum and BWF) were evaluated by using as cutoff points the values of the corresponding 90th percentile of CG and CBG taken together. Results A significant difference in albumin level (p<0.001) was noted in the BWF of patients with cancer compared with the other two groups. In addition, a significant difference in CEA level (p<0.001) was observed in the serum of cancer patients compared with the other two groups. The cutoff values for CEA in serum and albumin in BWF were 2.20 ng/mL and 2.00 g/dL, respectively. The areas under the corresponding ROC curves were 93% and 97%. Combination of CEA-serum and albumin-BWF by logistic regression analysis increased their diagnostic value. Conclusion Measurement of albumin levels in BWF could be a useful additional diagnostic tool to differentiate malignant from non-malignant lung diseases. Moreover, the combined measurement of CEA in serum and albumin in BWF could be of aid in the follow-up of lung cancer patients.

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