Preconditioning with PDE1 Inhibitors and Moderate-Intensity Training Positively Affect Systemic Redox State of Rats

Taken into consideration that oxidative stress response after preconditioning with phosphodiesterase inhibitors (PDEIs) and moderate physical activity has still not been clarified, the aim of this study was to assess the effects of PDEIs alone or in combination with physical activity, on systemic redox status. The study was carried out on 96 male Wistar albino rats classified into two groups. The first group included animals exposed only to pharmacological preconditioning (PreC) maneuver (sedentary control (CTRL, 1 ml/day saline, n=12), nicardipine (6 mg/kg/day of NIC, n=12), vinpocetine (10 mg/kg/day of VIN, n=12), and nimodipine (NIM 10 mg/kg/day of, n=12). The second included animals exposed to preconditioning with moderate-intensity training (MIT) on treadmill for 8 weeks. After 5 weeks from the start of training, the animals were divided into four subgroups depending on the medication to be used for pharmacological PreC: moderate-intensity training (MIT+ 1 ml/day saline, n=12), nicardipine (MIT+ 6 mg/kg/day of NIC, n=12), vinpocetine (MIT+ 10 mg/kg/day of VIN, n=12), and nimodipine (MIT+ 10 mg/kg/day of NIM, n=12). After three weeks of pharmacological preconditioning, the animals were sacrificed. The following oxidative stress parameters were measured spectrophotometrically: nitrites (NO2−), superoxide anion radical (O2−), hydrogen peroxide (H2O2), index of lipid peroxidation (TBARS), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH). Our results showed that PDE1 and MIT preconditioning decreased the release of prooxidants and improved the activity of antioxidant enzymes thus preventing systemic oxidative stress.

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Gardening Intervention as a Low- to Moderate-Intensity Physical Activity for Improving Blood Lipid Profiles, Blood Pressure, Inflammation, and Oxidative Stress in Women over the Age of 70: A Pilot Study

HortScience ◽

10.21273/hortsci11232-16 ◽

2017 ◽

Vol 52 (1) ◽

pp. 200-205 ◽

Cited By ~ 8

Author(s):

Sin-Ae Park ◽

A-Young Lee ◽

Hee-Geun Park ◽

Ki-Cheol Son ◽

Dae-Sik Kim ◽

...

Keyword(s):

Oxidative Stress ◽

Physical Activity ◽

Blood Pressure ◽

Blood Lipid ◽

Lipid Profiles ◽

Moderate Intensity ◽

Community Center ◽

Moderate Physical Activity ◽

Positive Effects ◽

And Oxidative Stress

The objective of this study was to investigate the effects of a gardening intervention as a physical activity in women aged over 70 years. Twenty-one women aged over 70 years were recruited from the community in Seoul, South Korea. Eleven subjects at a senior community center participated in a 15-session gardening program (twice a week, average 50 minutes per session) from Sept. to Nov. 2015. The rest of the subjects who were recruited from another senior community center acted as the control group. Blood lipid profiles, blood pressure, inflammation in peripheral-blood mononuclear cells (PBMC), and oxidative stress were assessed by a blood test before and after the 15-session gardening intervention. The results showed that the subjects in the gardening intervention as a low- to moderate-physical activity had a significant improvement in their high-density lipoprotein (HDL) level, systolic and diastolic blood pressures, and the variables related to immunity such as tumor necrosis factor-α (TNF-α) for inflammation in blood and receptor for advanced glycation end products (RAGE) expression for oxidative stress. The results of this study suggested that the 15-session gardening intervention as a low- to moderate-physical activity led to positive effects on the blood lipid profiles, blood pressure, level of inflammatory markers in blood, and oxidative stress of women aged over 70 years.

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Imaging NAD(H) Redox Alterations in Cryopreserved Alveolar Macrophages from Ozone-Exposed Mice and the Impact of Nutrient Starvation during Long Lag Times

Antioxidants ◽

10.3390/antiox10050767 ◽

2021 ◽

Vol 10 (5) ◽

pp. 767

Author(s):

He N. Xu ◽

Joanna Floros ◽

Lin Z. Li ◽

Shaili Amatya

Keyword(s):

Oxidative Stress ◽

Alveolar Macrophages ◽

Redox State ◽

Redox Status ◽

Nutrient Starvation ◽

Ozone Exposure ◽

Reduced Form ◽

Time Period ◽

Lag Times ◽

The Impact

Employing the optical redox imaging technique, we previously identified a significant redox shift of nicotinamide adenine dinucleotide (NAD and the reduced form NADH) in freshly isolated alveolar macrophages (AM) from ozone-exposed mice. The goal here was twofold: (a) to determine the NAD(H) redox shift in cryopreserved AM isolated from ozone-exposed mice and (b) to investigate whether there is a difference in the redox status between cryopreserved and freshly isolated AM. We found: (i) AM from ozone-exposed mice were in a more oxidized redox state compared to that from filtered air (FA)-exposed mice, consistent with the results obtained from freshly isolated mouse AM; (ii) under FA exposure, there was no significant NAD(H) redox difference between fresh AM that had been placed on ice for 2.5 h and cryopreserved AM; however, under ozone exposure, fresh AM were more oxidized than cryopreserved AM; (iii) via the use of nutrient starvation and replenishment and H2O2-induced oxidative stress of an AM cell line, we showed that this redox difference between cryopreserved and freshly isolated AM is likely the result of the double “hit”, i.e., the ozone-induced oxidative stress plus nutrient starvation that prevented freshly isolated AM from a full recovery after being on ice for a prolonged time period. The cryopreservation technique we developed eliminates/minimizes the effects of oxidative stress and nutrient starvation on cells. This method can be adopted to preserve lung macrophages from animal models or clinical patients for further investigations.

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Serum Albumin Redox States: More than Oxidative Stress Biomarker

Antioxidants ◽

10.3390/antiox10040503 ◽

2021 ◽

Vol 10 (4) ◽

pp. 503

Author(s):

Fuka Tabata ◽

Yasuaki Wada ◽

Satomi Kawakami ◽

Kazuhiro Miyaji

Keyword(s):

Oxidative Stress ◽

Serum Albumin ◽

Animal Studies ◽

Redox State ◽

Ex Vivo ◽

Pathological Condition ◽

Analytical Techniques ◽

Cysteine Residue ◽

Research Field ◽

Systemic Oxidative Stress

Serum albumin is the most abundant circulating protein in mammals including humans. It has three isoforms according to the redox state of the free cysteine residue at position 34, named as mercaptalbumin (reduced albumin), non-mercaptalbumin-1 and -2 (oxidized albumin), respectively. The serum albumin redox state has long been viewed as a biomarker of systemic oxidative stress, as the redox state shifts to a more oxidized state in response to the severity of the pathological condition in various diseases such as liver diseases and renal failures. However, recent ex vivo studies revealed oxidized albumin per se could aggravate the pathological conditions. Furthermore, the possibility of the serum albumin redox state as a sensitive protein nutrition biomarker has also been demonstrated in a series of animal studies. A paradigm shift is thus ongoing in the research field of the serum albumin. This article provides an updated overview of analytical techniques for serum albumin redox state and its association with human health, focusing on recent findings.

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Moderate physical activity reduces 10-year diabetes incidence: the mediating role of oxidative stress biomarkers

International Journal of Public Health ◽

10.1007/s00038-017-1052-8 ◽

2017 ◽

Vol 63 (2) ◽

pp. 297-305 ◽

Cited By ~ 3

Author(s):

Efi Koloverou ◽

◽

Konstantinos Tambalis ◽

Demosthenes B. Panagiotakos ◽

Ekavi Georgousopoulou ◽

...

Keyword(s):

Oxidative Stress ◽

Physical Activity ◽

Moderate Physical Activity ◽

Diabetes Incidence ◽

Oxidative Stress Biomarkers ◽

Mediating Role ◽

Stress Biomarkers

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Interaction of physical activity and diet: implications for haemostatic factors

Public Health Nutrition ◽

10.1017/s136898009900052x ◽

1999 ◽

Vol 2 (3a) ◽

pp. 383-390 ◽

Cited By ~ 5

Author(s):

Rainer Rauramaa ◽

SB Väisänen

Keyword(s):

Physical Activity ◽

Blood Coagulation ◽

Lifestyle Changes ◽

Regular Physical Activity ◽

Moderate Intensity ◽

Moderate Physical Activity ◽

Platelet Aggregability ◽

Moderate Intensity Physical Activity ◽

Intensity Physical Activity ◽

The Impact

AbstractRegular moderate intensity physical activity and habitual diet providing no more than one third of energy from fats have been recommended for the prevention of atherosclerotic diseases. The background for these guidelines is the key role of plasma lipids. However, the importance of thrombogenesis in acute myocardial infarction has become obvious during the last decade. Hyperlipidaemia and excess of adipose tissue increase platelet aggregability and blood coagulation, and decrease fibrinolysis. Both regular physical activity and dietary fat reduction decrease blood lipids and body fat thereby diminishing the risk of thrombosis. Currently, data on interactions between physical activity and diet on haemostasis are scarce, and the few studies available have not demonstrated additional effects when these two lifestyle modifications have been combined. This paper is restricted only to studies using controlled randomized design. Regular moderate intensity physical activity as well as diet rich in omega-3 fatty acids decrease platelet aggregability. The effects of regular physical activity on plasma fibrinogen remain contradictory, while the impact of diet is even less clear. Plasminogen activator inhibitor-1, a possible link between insulin resistance syndrome and coronary heart disease, may decrease due to physical training or low fat diet. It can be hypothesized that moderation in physical activity and diet carries a more powerful impact on blood coagulation and fibrinolysis than either lifestyle modification alone. Studies focusing on the interactions of regular moderate physical activity and fat-modified diet are needed in efforts to optimize the preventive actions by lifestyle changes.

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The Regulation of the Metastatic Cascade by Physical Activity: A Narrative Review

Cancers ◽

10.3390/cancers12010153 ◽

2020 ◽

Vol 12 (1) ◽

pp. 153 ◽

Cited By ~ 2

Author(s):

Sophie van Doorslaer de ten Ryen ◽

Louise Deldicque

Keyword(s):

Physical Activity ◽

Careful Analysis ◽

The Body ◽

Narrative Review ◽

Moderate Intensity ◽

Moderate Physical Activity ◽

Moderate Intensity Exercise ◽

Tumor Spread ◽

Warm Up ◽

Cancer Dissemination

The purpose of this narrative review is to provide an overview of the currently available knowledge about the mechanisms by which physical activity may affect metastatic development. The search terms exercise [Title/Abstract] AND metastasis [Title/Abstract] returned 222 articles on PUBMED on the 10 February 2019. After careful analysis of the abstracts, a final selection of 24 articles was made. Physical activity regulates the levels of metastatic factors in each of the five steps of the process. Moderate intensity exercise appears to prevent tumor spread around the body, among others, by normalizing angiogenesis, destroying circulating tumor cells, and decreasing endothelial cells permeability. Contrarily, high-intensity exercise seems to favor cancer dissemination, likely through excessive stress, which can be somewhat counteracted by an appropriate warm-up. In conclusion, chronic adaptations to moderate-intensity endurance exercise seem the most effective way to achieve a preventive effect of exercise on metastases. Altogether, the data gathered here reinforce the importance of encouraging cancer patients to perform moderate physical activity several times a week. To limit the undesired events thereof, a good knowledge of the patient’s training level is important to establish an adapted exercise training program.

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Sequential Analysis of Oxidative Stress Markers and Vitamin C Status in Acute Bacterial Osteomyelitis

Mediators of Inflammation ◽

10.1155/2014/975061 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Rade Grbic ◽

Dijana J Miric ◽

Bojana Kisic ◽

Ljiljana Popovic ◽

Vojkan Nestorovic ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Sequential Analysis ◽

Bone Cells ◽

Redox Status ◽

Oxidative Stress Markers ◽

Total Vitamin ◽

Baseline Serum ◽

Stress Markers ◽

Systemic Oxidative Stress

In bacterial bone infections, excessively formed oxidants may result in local and systemic oxidative stress. Vitamin C is the major extracellular nonenzymatic antioxidant, also implicated in bone cells metabolism and viability. The physiological functions of vitamin C largely depend on its redox status. We sequentially assessed oxidative stress markers, hydroperoxides and malondialdehyde (MDA), total antioxidant activity (AOA), total vitamin C, ascorbic acid (Asc), and oxidized/reduced vitamin C ratio in 137 patients with acute osteomyelitis (OM). Compared to 52 healthy controls, in OM group baseline serum hydroperoxides, MDA and oxidized/reduced vitamin C ratio were higher whilst Asc and AOA were lower (P < 0.05, resp.). On the other side, total vitamin C levels in patients and controls were similar(P > 0.05), thereby suggesting a relative rather than absolute vitamin C deficiency in OM. During the follow-up, oxidative stress markers, AOA, and oxidizedreduced vitamin C ratio were gradually returned to normal, while there was no apparent change of total vitamin C concentrations. Persistently high values of oxidized/reduced vitamin C ratio and serum MDA were found in subacute OM. In conclusion, acute OM was associated with enhanced systemic oxidative stress and the shift of vitamin C redox status towards oxidized forms.

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Significance of Elevated Blood Metal Ion Levels in Patients with Metal-on-Metal Prostheses: An Evaluation of Oxidative Stress Markers

The Open Orthopaedics Journal ◽

10.2174/1874325001004010221 ◽

2010 ◽

Vol 4 (1) ◽

pp. 221-227 ◽

Cited By ~ 21

Author(s):

Cathy Tkaczyk ◽

Alain Petit ◽

John Antoniou ◽

David J Zukor ◽

Maryam Tabrizian ◽

...

Keyword(s):

Oxidative Stress ◽

Metal Ion ◽

Biological Effects ◽

Redox Status ◽

Heme Oxygenase 1 ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Systemic Oxidative Stress ◽

Metal On Metal ◽

Significant Difference

It is widely known that cobalt and chromium ions can enhance the production of reactive oxygen species, known to be damaging to cells by disturbing their redox status and then generating oxidative stress. The aim of the present study was to determine if increased metal ion levels induce a state of oxidative stress in patients with metal-on-metal (MM) hip arthroplasty. Results indicated that there was no significant difference in the concentration of oxidative stress markers (total antioxidants, peroxides, and nitrated proteins) in the patients with MM bearings compared to patients without prostheses. The activity antioxidant enzymes was stable (catalase and glutathione peroxidase) or slightly decreased (superoxide dismutase and heme oxygenase-1) over time. This work is the first to determine the biological effects of metal ions released from MM hip implants with regards to mid-term systemic oxidative stress and showed that the increased levels of Co and Cr ions are not associated with significant oxidative stress damage in the plasma of patients with these implants.

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Effects of moderate and high intensity isocaloric aerobic training upon microvascular reactivity and myocardial oxidative stress in rats

10.1101/655530 ◽

2019 ◽

Author(s):

Lorena Paes ◽

Daniel Lima ◽

Cristiane Matsuura ◽

Maria das Graças de Souza ◽

Fátima Cyrino ◽

...

Keyword(s):

Oxidative Stress ◽

High Intensity ◽

Aerobic Training ◽

Training Group ◽

Moderate Intensity ◽

Sedentary Control ◽

Enos Expression ◽

Microvascular Reactivity ◽

Intensity Training ◽

Biomarkers Of Oxidative Stress

ABSTRACTSystemic and central cardiovascular adaptations may vary in response to chronic exercise performed with different intensities and volumes. This study compared the effects of aerobic training with different intensities but equivalent volume upon microvascular reactivity in cremaster muscle and myocardial biomarkers of oxidative stress in Wistar rats. After peak oxygen uptake (VO2peak) assessment, rats (n=24) were assigned into three groups: moderate-intensity exercise training (MI); high-intensity exercise training (HI); sedentary control (SC). Treadmill training occurred during 4 weeks, with exercise bouts matched by the energy expenditure (3.0-3.5 Kcal). Microvascular reactivity was assessed in vivo by intravital microscopy in cremaster muscle arterioles, while biomarkers of oxidative stress and eNOS expression were quantified at left ventricle and at aorta, respectively. Similar increasing vs. sedentary control group (SC) occurred in moderate intensity training group (MI) and high-intensity training group (HI) for endothelium-dependent vasodilation (10−4M: MI: 168.7%, HI: 164.6% vs. SC: 146.6%, P=0.0004). Superoxide dismutase (SOD) (HI: 0.13 U/mg vs. MI: 0.09 U/mg and SC: 0.06 U/mg; P=0.02), glutathione peroxidase (GPX) (HI: 0.00038 U/mg vs. MI: 0.00034 U/mg and SC: 0.00024 U/mg; P=0.04), and carbonyl protein content (HI: 0.04 U/mg vs. MI: 0.03 U/mg and SC: 0.01 U/mg; P=0.003) increased only in HI. No difference across groups was detected for catalase (CAT) (P=0.12), Thiobarbituric acid reactive substances (TBARS) (P = 0.38) or eNOS expression in aorta (P=0.44). In conclusion, higher exercise intensity induced greater improvements in myocardium antioxidant defenses, while gains in microvascular reactivity appeared to rely more on exercise volume than intensity.

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High-Salt Diet in the Pre- and Postweaning Periods Leads to Amygdala Oxidative Stress and Changes in Locomotion and Anxiety-Like Behaviors of Male Wistar Rats

Frontiers in Behavioral Neuroscience ◽

10.3389/fnbeh.2021.779080 ◽

2022 ◽

Vol 15 ◽

Author(s):

Pedro Ernesto de Pinho Tavares Leal ◽

Alexandre Alves da Silva ◽

Arthur Rocha-Gomes ◽

Tania Regina Riul ◽

Rennan Augusto Cunha ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Redox State ◽

Redox Status ◽

High Salt ◽

Standard Diet ◽

High Salt Diet ◽

Salt Diet ◽

Plus Maze ◽

Male Wistar Rats

High-salt (HS) diets have recently been linked to oxidative stress in the brain, a fact that may be a precursor to behavioral changes, such as those involving anxiety-like behavior. However, to the best of our knowledge, no study has evaluated the amygdala redox status after consuming a HS diet in the pre- or postweaning periods. This study aimed to evaluate the amygdala redox status and anxiety-like behaviors in adulthood, after inclusion of HS diet in two periods: preconception, gestation, and lactation (preweaning); and only after weaning (postweaning). Initially, 18 females and 9 male Wistar rats received a standard (n = 9 females and 4 males) or a HS diet (n = 9 females and 5 males) for 120 days. After mating, females continued to receive the aforementioned diets during gestation and lactation. Weaning occurred at 21-day-old Wistar rats and the male offspring were subdivided: control-control (C-C)—offspring of standard diet fed dams who received a standard diet after weaning (n = 9–11), control-HS (C-HS)—offspring of standard diet fed dams who received a HS diet after weaning (n = 9–11), HS-C—offspring of HS diet fed dams who received a standard diet after weaning (n = 9–11), and HS-HS—offspring of HS diet fed dams who received a HS diet after weaning (n = 9–11). At adulthood, the male offspring performed the elevated plus maze and open field tests. At 152-day-old Wistar rats, the offspring were euthanized and the amygdala was removed for redox state analysis. The HS-HS group showed higher locomotion and rearing frequency in the open field test. These results indicate that this group developed hyperactivity. The C-HS group had a higher ratio of entries and time spent in the open arms of the elevated plus maze test in addition to a higher head-dipping frequency. These results suggest less anxiety-like behaviors. In the analysis of the redox state, less activity of antioxidant enzymes and higher levels of the thiobarbituric acid reactive substances (TBARS) in the amygdala were shown in the amygdala of animals that received a high-salt diet regardless of the period (pre- or postweaning). In conclusion, the high-salt diet promoted hyperactivity when administered in the pre- and postweaning periods. In animals that received only in the postweaning period, the addition of salt induced a reduction in anxiety-like behaviors. Also, regardless of the period, salt provided amygdala oxidative stress, which may be linked to the observed behaviors.

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