scholarly journals Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 140 ◽  
Author(s):  
Osama A. A. Ahmed ◽  
Usama A. Fahmy ◽  
Rana Bakhaidar ◽  
Mohamed A. El-Moselhy ◽  
Solomon Z. Okbazghi ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Gastric Injury ◽  
Tween 20 ◽  
Protective Effects ◽  
Omega 3 ◽  
Ulcer Index ◽  
Ulcer Disease ◽  
Mucosal Defect ◽  
Protection Efficacy ◽  
Mixture Experimental Design

Peptic ulcer disease is an injury of the alimentary tract that leads to a mucosal defect reaching the submucosa. This study aimed to formulate and optimize omega-3 oil as a self-nanoemulsifying drug delivery system (SNEDDS) to achieve oil dispersion in the nano-range in the stomach to augment omega-3 oil gastric ulcer protection efficacy. Three SNEDDS components were selected as the design factors: the concentrations of the oil omega-3 (X1, 10–30%), the surfactant tween 20 and Kolliphor mixture (X2, 20–40%), and the cosurfactant transcutol (X3, 40–60%). The mixture experimental design proposed twenty-three formulations with varying omega-3 SNEDDS formulation component percentages. The optimized omega-3 SNEDDS formula was investigated for gastric ulcer protective effects by evaluating the ulcer index and by the determination of gastric mucosa oxidative stress parameters. Results revealed that optimized omega-3-SNEDDS achieved significant improvement in the gastric ulcer index in comparison with pure omega-3 oil. Histopathological findings confirmed the protective effect of the formulated optimized omega-3 SNEDDS in comparison with omega-3 oil. These findings suggest that formulation of omega-3 in the form of a SNEDDS would be more effective in gastric ulcer protection than the administration of omega-3 as a crude oil.

scholarly journals Optimized Self-Nanoemulsifying Delivery System Based on Plant-Derived Oil Augments Alpha-Lipoic Acid Protective Effects Against Experimentally Induced Gastric Lesions

Dose-Response ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 155932582110012
Author(s):  
Shaimaa M. Badr-Eldin ◽  
Usama A. Fahmy ◽  
Hibah M. Aldawsari ◽  
Osama A. A. Ahmed ◽  
Nabil A. Alhakamy ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Delivery System ◽  
Lipoic Acid ◽  
Tween 80 ◽  
Protective Effects ◽  
Alpha Lipoic Acid ◽  
Ulcer Index ◽  
Ulcer Disease ◽  
Mucosal Defect ◽  
Polyethylene Glycol 200

Peptic ulcer disease is an injury of the alimentary tract that leads to a mucosal defect reaching the submucosa. Alpha-lipoic acid (ALA), a natural potent antioxidant, has been known as a gastroprotective drug yet its low bioavailability may restrict its therapeutic efficacy. This study aimed to formulate and optimize ALA using a self-nanoemulsifying drug delivery system (SNEDDS) with a size of nano-range, enhancing its absorption and augmenting its gastric ulcer protection efficacy. Three SNEDDS components were selected as the design factors: the concentrations of the pumpkin oil (X1, 10–30%), the surfactant tween 80 (X2, 20–50%), and the co-surfactant polyethylene glycol 200 (X3, 30–60%). The experimental design for the proposed mixture produced 16 formulations with varying ALA-SNEDDS formulation component percentages. The optimized ALA-SNEDDS formula was investigated for gastric ulcer protective effects by evaluating the ulcer index and by the determination of gastric mucosa oxidative stress parameters. Results revealed that optimized ALA-SNEDDS achieved significant improvement in gastric ulcer index in comparison with raw ALA. Histopathological findings confirmed the protective effect of the formulated optimized ALASNEDDS in comparison with raw ALA. These findings suggest that formulation of ALA in SNEDDS form would be more effective in gastric ulcer protection compared to pure ALA.

scholarly journals Optimized Ellagic Acid–Ca Pectinate Floating Beads for Gastroprotection against Indomethacin-Induced Gastric Injury in Rats

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1006 ◽  
Author(s):  
Nabil A. Alhakamy ◽  
Shaimaa M. Badr-Eldin ◽  
Osama A. A. Ahmed ◽  
Abdulrahman A. Halwani ◽  
Hibah M. Aldawsari ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ellagic Acid ◽  
Gastric Ulcers ◽  
Gastric Injury ◽  
Parameter Determination ◽  
Ulcer Index ◽  
Oxidative Stress Parameter ◽  
Mucosal Defect ◽  
Inflammatory Drugs

A peptic ulcer is an alimentary tract injury that leads to a mucosal defect reaching the submucosa. This work aimed to optimize and maximize ellagic acid (EA) loading in Ca pectinate floating beads to maximize the release for 24 h. Three factors were selected: Ca pectinate concentration (X1, 1–3 w/v %), EA concentration (X2, 1–3 w/v %) and the dropping time (X3, 10–30 min). The factorial design proposed eight formulations. The optimized EA–Ca pectinate formulation was evaluated for the gastric ulcer index and the oxidative stress parameter determination of gastric mucosa. The results indicated that the optimum EA–Ca pectinate formula significantly improved the gastric ulcer index in comparison with raw EA. The protective effect of the optimized EA–Ca pectinate formula was further indicated by the histopathological features of the stomach. The results of the study indicate that an EA formulation in the form of Ca pectinate beads would be effective for protection against gastric ulcers because of Nonsteroidal anti-inflammatory drugs (NSAID) administration.

Effect of heat-killed Enterococcus faecalis EF-2001 on ethanol-induced acute gastric injury in mice: Protective effect of EF-2001 on acute gastric ulcer

2020 ◽  
Vol 39 (5) ◽  
pp. 721-733 ◽  
Author(s):  
D-B Jeon ◽  
H-G Shin ◽  
B-W Lee ◽  
S-H Jeong ◽  
J-H Kim ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Enterococcus Faecalis ◽  
Interleukin 1 ◽  
Mucosal Injury ◽  
Underlying Mechanism ◽  
Gastric Injury ◽  
Protective Effects ◽  
Necrosis Factor Alpha ◽  
Mitogen Activated Protein ◽  
Consequent Reduction

Enterococcus faecalis is a facultative anaerobic gram-positive commensal bacterium common in the gastrointestinal tract of animals and humans. This study aimed to investigate the protective effects of heat-killed E. faecalis EF-2001 (EF-2001) on acute gastric ulcer using a murine model of ethanol (EtOH)-induced acute gastric injury. EF-2001 (20, 40, and 80 mg/kg/day) was administered by oral gavage for 5 days before EtOH treatment (10 mL/kg body weight). EF-2001 effectively attenuated EtOH-induced gastric mucosal injury with reduced gastric mucosal ulcer and histological damage score. Pretreatment of EF-2001 markedly suppressed the phosphorylation of mitogen-activated protein kinases (MAPKs; ERK1/2, JNK, and p38MAPK). In addition, EF-2001 significantly inhibited phosphorylation of nuclear factor kappa B (NF-κB) and subsequently suppressed the upregulation of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor alpha, interleukin 1 beta, and interleukin 6 in gastric tissues. Taken together, these results suggest that EF-2001 exerts a gastroprotective effect against acute gastric injury, and the underlying mechanism might be associated with the suppression of MAPKs and NF-κB signaling and consequent reduction of pro-inflammatory mediators or cytokines.

scholarly journals EFFECT OF PHARMACOLOGICAL DOSES OF GARLIC AND OMEGA 3 ON GASTRIC LESIONS INDUCED BY ETHANOL IN MICE

2016 ◽  
Vol 9 (5) ◽  
pp. 153
Author(s):  
Ausama Ayoob Jaccob
Keyword(s):  
Gastric Ulcer ◽  
Gastric Ulceration ◽  
Gastric Lesions ◽  
Omega 3 ◽  
Control Groups ◽  
Ulcer Index ◽  
Once Daily ◽  
Garlic Oil ◽  
Induction Process ◽  
Area Increase

ABSTRACTObjective: This work was designed to investigate and compare the possible protective effect of pharmacological doses of garlic and omega 3 againstgastric lesions induced by ethanol in mice.Methods: A total of 30 mice involved in the study were divided into five groups with 6 mice for each. Groups 1 (ulcer control) and 2 (normal control),Groups 3 (positive ulcer control treated with ranitidine 50 mg/kg), Groups 4 and 5 treated with garlic oil and omega 3 oil at doses 200 and 150 mg/kg,respectively. All groups treated orally by gastric gavage once daily for 14 days before starting gastric ulcer (GU) induction process by absolute ethanoladministration.Results: A treatment with garlic and omega 3 ameliorated the severity of gastric ulceration evidenced by reduced ulcer index area, increase %gastroprotection, increase mucus content, reduced erosions, and necrosis.Conclusion: Oral administration of pharmacological doses of garlic oil and omega 3 oil shows significant gastroprotection against GU models inducedby absolute ethanol confirmed by biochemical and histological data.Keywords: Gastric ulcer, Garlic, Omega 3, Gastroprotection.

scholarly journals Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Jun Zhang ◽  
Zai-Lin Fu ◽  
Zhao-Xing Chu ◽  
Bi-Wei Song
Keyword(s):  
Superoxide Dismutase ◽  
Gastric Ulcer ◽  
Medicinal Plant ◽  
Oral Administration ◽  
Protein Content ◽  
Gastric Injury ◽  
Gastric Tissue ◽  
Gastroprotective Activity ◽  
Ulcer Index ◽  
Antiulcer Drug

Background. Abelmoschus manihot (L.) Medic flower is a medicinal plant for the treatment of diseases in China. The present study was carried out to scientifically validate the gastroprotective activity and clarify the possible mechanism of the total flavones from Abelmoschus manihot (L.) Medic flowers (TFA). Methods. Gastric ulcer was induced in mice by oral administration of ethanol. The gastroprotective activity of TFA was evaluated by the gastric ulcer index and histological examinations. The gastric tissue was collected in the form of homogenate. The level of malondialdehyde (MDA) and glutathione (GSH), the activity of superoxide dismutase (SOD), and protein content were measured. Western blotting for the expression of Bax, Bcl-2, TNF-α, and NF-κB(p65) was also carried out. The effect of TFA was compared with that of standard antiulcer drug omeprazole (100 mg/kg). Results. This gastroprotective effect of TFA could be attributed to the increase in the activity of SOD and GSH and decrease in the levels of MDA and also decrease in the levels of Bax, TNF-α, and NF-κB(p65) expressions and increase in the Bcl-2 expression level. Conclusion. The findings of this study demonstrated that TFA could significantly attenuate ethanol-induced gastric injury via antioxidative, anti-inflammatory, and antiapoptotic effects.

scholarly journals Antiulcerogenic effect of Capparis cartillaginea decne on indomethacin induced gastric ulcer in wistar rats

2020 ◽  
Vol 8 (10) ◽  
pp. 3445
Author(s):  
Nancy W. Mugo ◽  
Christine Wangia ◽  
Gideon Kikuvi ◽  
Samuel Ngugi
Keyword(s):  
Gastric Juice ◽  
Plant Extracts ◽  
Wistar Rats ◽  
Muscularis Mucosa ◽  
Total Acidity ◽  
Ulcer Index ◽  
Ulcer Disease ◽  
Mucosal Defect ◽  
Gastrointestinal Obstruction ◽  
Life Threatening

Background: Peptic ulcer disease is a non-malignant, mucosal lesion of the stomach or duodenum. The mucosal defect reaches the muscularis mucosa and sometimes, beyond causing life threatening complications, including haemorrhage, perforations, gastrointestinal obstruction and malignancy.Methods: The animals were pre-treated with omeprazole 20 mg/kg and 300 mg/kg of Capparis cartillaginea decne orally for 14 days. On the 15th day, ulcers were induced using indomethacin 30 mg/kg and 4 hours post ulcer induction, they were sacrificed. Ulcer index, pH, total acidity and volume were determined.Results: Extensive lesions were seen in indomethacin ulcerated rats with mean ulcer score of (1.260±0.18). In comparison, there were minimal areas of erosion on animals pre-treated with omeprazole (0.14±0.025) and plant extracts (0.280±0.097). Indomethacin-induced ulcer treated animals showed the highest volume of gastric juice output (3.14±0.21 ml), whereas the animals pre-treated with omeprazole had lower gastric juice output (2.20±0.2 9ml). This was comparable to animals pre-treated with the plant extract (1.80±0.13 ml). The pH was high in animals pre-treated with omeprazole (5.02±0.53). This was also seen in animals pre-treated with the extract (4.82±0.31). This was in comparison to the low pH seen in indomethacin ulcerated animals (2.20±0.16). Indomethacin-induced ulcer treated animals showed high levels of total acidity (88.64±1.71 mEq/L). Whereas the animals pre-treated with omeprazole had lower total acidity (55.26±3.77 mEq/L), which was also mirrored in animals pre-treated with the plant extracts (61.44±2.42 mEq/L).Conclusions: The extracts of Capparis cartillaginea decne showed anti-ulcer effect on indomethacin induced ulcers in Wistar rats.

scholarly journals Protective effects of a unique combination of nutritionally active ingredients on risk factors and gene expression associated with atherosclerosis in C57BL/6J mice fed a high fat diet

2021 ◽  
Author(s):  
Joe W. E. Moss ◽  
Jessica O Williams ◽  
Wijdan Al-Ahmadi ◽  
Victoria O'Morain ◽  
Yee-Hung Chan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Risk Factors ◽  
Cardiovascular Disease ◽  
High Fat Diet ◽  
Inflammatory Disorder ◽  
Protective Effects ◽  
Unique Combination ◽  
Omega 3 ◽  
High Fat ◽  
Food Ingredients

Atherosclerosis, an inflammatory disorder of the vasculature and the underlying cause of cardiovascular disease, is responsible for one in three global deaths. Consumption of active food ingredients such as omega-3...

Role of duodenal reflux in nonglandular gastric ulcer disease of the mature horse

2010 ◽  
Vol 31 (S29) ◽  
pp. 24-29 ◽  
Author(s):  
HELEN M. BERSCHNEIDER ◽  
A. T. BLIKSLAGER ◽  
M. C. ROBERTS
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Disease ◽  
Duodenal Reflux

scholarly journals Evaluation of a poly-herbal preparation for the treatment of peptic ulcer

2017 ◽  
Vol 12 (2) ◽  
pp. 11
Author(s):  
Zaman Hayat ◽  
Mueen Ahmad Chaudhry ◽  
. Alamgeer
Keyword(s):  
Peptic Ulcer ◽  
Gastric Ulcer ◽  
Treated Group ◽  
Ulcer Formation ◽  
Ulcer Index ◽  
Herbal Formulation ◽  
High K ◽  
Ulcer Model ◽  
Dose Dependent ◽  
Gross Examination

<p class="Abstract">The aim of this study was to validate the traditional uses of ulcerene, a poly-herbal formulation in ethanol, aspirin and stress-induced gastric ulcer model of rat. The extent of gastric ulcer formation was studied, using ulcer score, ulcer index, percentage cure through gross examination and histopathological evaluation. A significant (p&lt;0.001) dose-dependent anti-ulcerant effect was observed in ulcerene (50 and 100 mg/kg)-treated group with highest effectiveness against ethanol-induced ulcer. The concentration-dependent spasmolytic effect was seen in spontaneously contracting, high K<sup>+</sup> (80 mM) and carbachol (1 µM)-induced jejunum contractions (10, 0.3 and 1 mg/mL), similar to dicyclomine (10, 1 and 3 µM), indicated non-specific spasmolytic mechanism behind the effect. By considering these results, ulcerene can be suggested for the treatment of peptic ulcer.</p>

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