scholarly journals The Role of miRNA-146a and Proinflammatory Cytokines in Carotid Atherosclerosis

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Pan Huang ◽  
Xiao-ying He ◽  
Min Xu
Keyword(s):  
Peripheral Blood ◽  
Vulnerable Plaque ◽  
Carotid Atherosclerosis ◽  
Area Under The Curve ◽  
Normal Group ◽  
Expression Levels ◽  
Plaque Type ◽  
Tnf Α ◽  
Stable Plaque

The aim of this study was to investigate the expression and significance of miRNA-146a in peripheral blood of patients with carotid atherosclerosis (CAS). Patients with CAS were selected as the stenosis (CAS) group ( n = 180 ). According to the degree of stenosis, they were divided into the mild stenosis group (MS group, n = 64 ), moderate stenosis group (M group, n = 62   cases ), and severe stenosis group (SS group, n = 54 ). According to the plaque type, patients were divided into a stable plaque group (SP group, n = 96 ) and a vulnerable plaque group (VP group, n = 84 ). Patients without CAS represented the normal group ( n = 90 ). The expression levels of miRNA-146a as well as IL-6 and TNF-α in peripheral blood were detected by RT-PCR and ELISA, respectively. The expression levels of miRNA-146a, IL-6, and TNF-α in the CAS group were higher than those in the normal group and positively correlated with the degree of stenosis and plaque vulnerability. The expression levels of miRNA-146a, IL-6, and TNF-α in the stable plaque group were lower than those in the vulnerable plaque group. The area under the curve of miRNA-146a predicting the vulnerability of plaques was significant at 0.641. The expression level of miRNA-146a in the CAS group was positively correlated with IL-6 and TNF-α levels. Our results indicate that miRNA-146a may participate in the occurrence and development of CAS by regulating the expression of IL-6 and TNF-α, which may be potential biomarkers of CAS.

scholarly journals Expression Analysis of Circulating miR-22, miR-122, miR-217 and miR-367 as Promising Biomarkers of Acute Lymphoblastic Leukemia

2021 ◽  
Author(s):  
Fatemeh hosseinpour-soleimani ◽  
Gholamreza Khamisipour ◽  
Zahra Derakhshan ◽  
Bahram Ahmadi
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Lymphoblastic Leukemia ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Healthy Individuals ◽  
Quantitative Real Time Pcr ◽  
Expression Levels

Abstract Background Currently, the role of serum-based biomarkers such as microRNAs in cancer diagnosis has been extensively established. This study aimed to determine expression levels of bioinformatically selected miRNAs and whether they can be used as biomarkers or a new therapeutic target in patients with Acute Lymphoblastic Leukemia (ALL). Materials and Methods The expression levels of serum miR-22, miR-122, miR-217, and miR-367 in 21 ALL patients and 21 healthy controls were measured using quantitative real-time PCR. The receiver operating characteristic (ROC) curve and the associated area under the curve (AUC) was used to assess candidate miRNAs' diagnostic value as a biomarker. Results The results showed that miR-217 was markedly decreased in patients with ALL compared to controls. Moreover, miR-22, miR-122, and miR-367 were found to be upregulated. Furthermore, ROC analysis showed that serum miR-217 and miR-367 could differentiate ALL patients from the healthy individuals, while miR-22 has approximate discriminatory power that requires further investigation. Conclusion Collectively, the results suggested that miR-217 may play a tumor suppressor role in ALL, whereas miR-22, miR-122, and miR-367 could function as an oncogene. Overall, miR-22, miR-217, and miR-367 could be considered possible biomarkers for the early diagnosis of ALL.

Expression and Significance of microRNA-3686 in Peripheral Blood of Patients with Refractory Epilepsy

2019 ◽  
Vol 9 (11) ◽  
pp. 1507-1511
Author(s):  
Pan Huang ◽  
Xiao-Ying He ◽  
Min Xu
Keyword(s):  
Diagnostic Criteria ◽  
Roc Curve ◽  
Peripheral Blood ◽  
Refractory Epilepsy ◽  
Area Under The Curve ◽  
Normal Group ◽  
Control Group ◽  
Experimental Group

The study is to investigate the expression and significance of microRNA-3686 in peripheral blood of refractory epilepsy patients (RE). 83 patients with untreated epilepsy were selected as the experimental group. During the treatment, According to diagnostic criteria for refractory epilepsy, 15 patients were diagnosed with refractory epilepsy (RE group) and 68 cases in no refractory epilepsy group (NRE group). 50 patients in the normal group were selected as the control group. The results showed that the expression of microRNA-3686 in the peripheral blood of the experimental group was lower than that of the control group (P < 0.05). The levels of P-gp and MRP1 in peripheral blood of patients in RE group were higher than those in NRE group (P < 0.05). microRNA-3686 was negatively correlated with P-gp (r= – 0.799, P < 0.05), and had no correlation with MRP1 (r= 0.279, P= 0.314); ROC curve showed microRNA-3686 prediction RE of the area under the curve of epilepsy AUC was 0.733, 95% CI (0.603–0.863), (P < 0 05). In Conclusion the microRNA-3686 is low in peripheral blood of patients with refractory epilepsy and may be involved in the resistance of epilepsy by regulating P-gp, but not MRP1.

scholarly journals Relationship between serum microRNA-223 and microRNA-126 and plaque stability in patients with carotid atherosclerosis

2020 ◽  
Author(s):  
Ying zhang ◽  
Jian li ◽  
Jie li ◽  
Lin ma ◽  
Shuhua zhang
Keyword(s):  
Carotid Atherosclerosis ◽  
Characteristic Curve ◽  
Related Factors ◽  
Diagnostic Efficacy ◽  
Expression Levels ◽  
Plaque Instability ◽  
Plaque Stability ◽  
Serum Microrna ◽  
Direct Target Gene ◽  
Stable Plaque

Abstract Background Numerous researches have demonstrated the essential functions of microRNAs (miRNAs) in cardiovascular disease. Herein, we want to probe into the roles of miR-126 and miR-223 in the prediction of plaque stability of carotid atherosclerosis (CA). Methods First, miRNA microarray was performed to analyze the differentially expressed miRNA in serum of normal controls and CA patients. Next, the differential expression of miR-223 and miR-126 in CA was verified and the correlations of their expression levels with plaque stability-related factors were analyzed. Then, the predictive efficacy of miR-223 and miR-126 on plaque stability was analyzed by receiver operating characteristic curve, then the targeting relationships of miR-223 and miR-126 with COX2 were verified by online prediction and luciferase activity assay. Finally, the relationship between COX2 expression and CA plaque stability was analyzed. Results Expression levels of miR-223 and miR-126 were decreased in the serum of CA patients and they had good diagnostic efficacy for CA. Additionally, we found that the expression levels of miR-223 and miR-126 in the serum of CA patients with unstable plaque were lower than those with stable plaque. miR-223 and miR-126 were negatively correlated with plaque instability-related indicators, while COX2, a direct target gene of miR-223 and miR-126, was positively related to plaque instability-related indicators. Conclusion This study indicates that miR-223 and miR-126 are lowly expressed in serum of CA patients, which can be used as a clinical indicator for predicting the plaque stability in CA patients.

scholarly journals Role of some serum biomarkers in the early detection of diabetic cardiomyopathy

2021 ◽  
pp. FSO682
Author(s):  
Amany H Abdelrahman ◽  
Iman I Salama ◽  
Somaia I Salama ◽  
Dalia M Elmosalami ◽  
Mona H Ibrahim ◽  
...  
Keyword(s):  
Early Detection ◽  
Diastolic Dysfunction ◽  
Diabetic Cardiomyopathy ◽  
Systolic Dysfunction ◽  
Area Under The Curve ◽  
Serum Biomarkers ◽  
Control Groups ◽  
Tnf Α

Aim: To assess the role of serum biomarkers in early prediction of diabetic cardiomyopathy. Materials and methods: The participants were three groups of Type 2 diabetes mellitus (DM) patients having diastolic dysfunction (DM-DD), systolic dysfunction (DM-SD) and normal echocardiography (DM-N) with two control groups: non-DM diastolic dysfunction patients (DD) and healthy controls. AGEs, TNF-α, IL-6, IGFBP-7, creatinine and insulin were assessed. Results: TNF-α, AGEs, creatinine and insulin panel had area under the curve (AUC) of 0.913 in distinguishing DM-DD from DM-N (78.7% sensitivity and 100% specificity). IL-6 and AGEs panel had AUC 0.795 for differentiating DM-SD from DM-DD (90.6% sensitivity). IL-6, TNF-α and AGEs panel had AUC 0.924 for differentiating diabetic cardiomyopathy from DM-N (85% sensitivity and specificity). Conclusion: A panel of AGEs, IL-6, TNF-α, insulin and creatinine might be used for early detection of DM-DD among T2DM patients.

scholarly journals Leucine-rich α2-glycoprotein-1 upregulation in plasma and kidney of patients with lupus nephritis

2020 ◽  
Author(s):  
Yi Yang ◽  
Ran Luo ◽  
Yichun Cheng ◽  
Tingting Liu ◽  
Wei Dai ◽  
...  
Keyword(s):  
Renal Function ◽  
Lupus Nephritis ◽  
Disease Activity ◽  
Peripheral Blood ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epithelial Cell Line ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Expression Levels

Abstract Background Increased leucine-rich α2-glycoprotein-1 (LRG1) has been observed in various inflammatory and autoimmune diseases. We aimed to explore the expression and role of LRG1 in lupus nephritis (LN). Methods Plasma LRG1 (pLRG1) was measured by enzyme-linked immunosorbent assay in 101 patients with renal biopsy-proven LN and 21 healthy controls (HC). Relationships between pLRG1 and clinical and pathological characteristics were analyzed. The expression of LRG1 in peripheral blood leukocytes and kidney was detected by flow cytometry, immunohistochemistry and immunofluorescence, respectively. Further cell experiments were focused on the role of LRG1. Results We found that LRG1 was expressed in plasma, some peripheral blood leukocytes, proximal tubule and several inflammatory cells. The levels of LRG1 in plasma, peripheral blood leukocytes and kidney were elevated in LN patients as compared to HC. Plasma expression levels of LRG1 correlated positively with renal function and renal disease activity, and reflect specific pathologic lesions in the kidneys of patients with LN. Interleukin-1β and interleukin-6, not tumor necrosis factor-α and interferon γ induced the LRG1 expression in human renal tubular epithelial cell line. Moreover, stimulation of recombinant human LRG1 could inhibit late apoptosis, promote proliferation and regulate expression of inflammatory factors and cytokines. Conclusions Plasma expression levels of LRG1 were associated with renal function, disease activity, and pathology in LN. It might also be involved in renal inflammation, proliferation and apoptosis of endothelial cells. LRG1 might be a potential prognosis novel predictor in LN patients.

scholarly journals NOCTURNIN Gene Diurnal Variation in Healthy Volunteers and Expression Levels in Shift Workers

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Massimo Bracci ◽  
Alfredo Copertaro ◽  
Veronica Ciarapica ◽  
Mariella Barbaresi ◽  
Stefano Esposito ◽  
...  
Keyword(s):  
Diurnal Variation ◽  
Healthy Volunteers ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Shift Workers ◽  
Expression Levels ◽  
Blood Lymphocytes ◽  
The Relationship

Objective. The NOCTURNIN gene links nutrient absorption and metabolism to the circadian clock. Shift workers are at a heightened risk of overweight and of developing obesity and metabolic syndrome. This study investigates the diurnal variation of NOCTURNIN in healthy volunteers and its expression levels in rotational shift and daytime workers. Methods. NOCTURNIN expression levels were evaluated in peripheral blood lymphocytes from 15 healthy volunteers at 4-hour intervals for 24 h. Metabolic parameters and NOCTURNIN expression were measured in workers engaged in shift and daytime work. Results. In the group of volunteers NOCTURNIN expression showed diurnal variation, with a peak at 8:00 AM. NOCTURNIN expression was higher in shift workers than in daytime workers. Multivariate analysis confirmed the role of shift work as an independent factor affecting NOCTURNIN expression. Notably, its level correlated directly with body mass index and inversely with total energy expenditure. Conclusions. Measuring NOCTURNIN expression levels in human peripheral blood lymphocytes can improve investigations on the relationship between changes in circadian rhythm and metabolic disorders. Shift workers show higher NOCTURNIN levels than daytime workers.

The Role of Sulfates in Fucoidan Extracted from Fucus evanescens in Proinflammatory Cytokines Production by Human Peripheral Blood Cells in vitro

2020 ◽  
Vol 65 (5-6) ◽  
pp. 3-10
Author(s):  
S. R. Khil'chenko ◽  
T. S. Zaporozhets ◽  
T. N. Zvyagintseva ◽  
N. M. Shevchenko ◽  
N. N. Besednov
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Brown Algae ◽  
Biological Activities ◽  
Molecular Structures ◽  
Sulfated Polysaccharides ◽  
Peripheral Blood Cells ◽  
Fucus Evanescens ◽  
Tnf Α

Fucoidans, sulfated polysaccharides extracted from brown algae (Phaeophyceae), have a wide spectrum of bioactivity. Studies of molecular structures of fucoidans and deciphering of molecular elements' impact on their biological activities are at their active stage. The article shows the role of sulfates and acetyl groups in fucoidan isolated from Fucus evanescens in proinflammatory cytokines production by human heparinized unfractionated peripheral blood cells. Material and Methods. The cells were incubated with native fucoidan (N) and its deacetylated (deA), partially desulfated (deS), and both deacetylated and partially desulfated (deAdeS) derivatives (100 μg/mL). Cytokine concentrations were determined in cell supernatants by ELISA in a 'sandwich' modification with commercial kits. Results. Incubation with N fucoidan led to an increase of IL-6, TNF-α, IL-8 levels in supernatants. Partial removal of sulfate groups cancelled or decreased stimulating effect for IL-6, TNF-α, cytokines, but not for IL-8. deAc fucoidan action was comparable with N polysaccharide. Native polysaccharide and its chemically modified derivatives did not change IFN-γ и IL-10 cytokine production. Conclusion. The obtained results suggest that sulfates have a significant role in cytokine-producing properties of fucoidan extracted from brown algae F.evanescens.

scholarly journals Comparative Study of Protein Expression Levels of Five Plaque Biomarkers and Relation with Carotid Plaque Type Classification in Patients after Carotid Endarterectomy

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Konstantinos Kyriakidis ◽  
Pavlos Antoniadis ◽  
Sohail Choksy ◽  
Rigini M. Papi
Keyword(s):  
Protein Expression ◽  
Vulnerable Plaque ◽  
Carotid Plaque ◽  
Plaque Rupture ◽  
Pearson Correlation ◽  
Plaque Morphology ◽  
Expression Levels ◽  
Plaque Type ◽  
Type Iv ◽  
Patients At Risk

Atherosclerosis is an inflammatory process resulting in local plaque deposition in the vessel wall of arteries with symptoms to various areas of vascular tree. Identification of patients with progressive advanced atherosclerotic disease is mainly based on the known characteristics of the vulnerable or recently ruptured plaque. Molecular and cellular features associated with the vulnerable plaque are considered potential diagnostic markers for plaque rupture and thrombosis. Here, protein expression levels of the metalloproteases MMP-1, MMP-9, osteopontin (OPN), and cytokines TNFα and IL-6 in tissue extracts of carotid plaques in patients after endarterectomy were estimated by Western immunoblotting, after SDS-PAGE analysis and evaluated based on the ultrasonographic plaque morphology. The gender and age effect was also examined. MMP-1, MMP-9, and IL-6 were expressed in higher levels compared to OPN and TNFa as well as in symptomatic (with type II and III carotid plaque classification) than asymptomatic (type IV) patients with differences considered statistically significant (P values <0.05). A significant positive correlation between MMP-1 and IL-6 (with Pearson correlation coefficient 0.748) is also notable. The data give further insight into the possible role of specific biomarker and enhance the need for further studies in order to clarify the proper one(s) for detection of the vulnerable plaque and help identify patients at risk for cardiovascular events.

scholarly journals Acupuncture Can Regulate the Distribution of Lymphocyte Subsets and the Levels of Inflammatory Cytokines in Patients With Mild to Moderate Vascular Dementia

2021 ◽  
Vol 13 ◽  
Author(s):  
Hui Zhi ◽  
Yao Wang ◽  
Shichen Chang ◽  
Pan Pan ◽  
Zhenzhen Ling ◽  
...  
Keyword(s):  
T Cells ◽  
Immune Function ◽  
Vascular Dementia ◽  
Inflammatory Cytokines ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Acupuncture Treatment ◽  
Normal Group ◽  
Tnf Α ◽  
Ifn Γ

Background: Vascular dementia (VD) is the second most common type of dementia after Alzheimer’s disease, but there is a lack of definitive treatment for VD. Acupuncture treatment is effective in improving the cognitive impairment and behavioral capacity of patients with VD. In recent years, more studies indicated that peripheral inflammation and abnormal peripheral immune function may aggravate neuroinflammation and cognitive dysfunction. However, there are few studies about the acupuncture and the abnormal peripheral immune function of VD. Also, few studies concern the regulating effect of acupuncture on peripheral immunity of patients with VD.Objective: The aim of this study was to explore the effect of the “sanjiao” acupuncture method on peripheral immunity of patients with mild to moderate VD.Methods: A total of 30 patients with VD were involved in the acupuncture group (AG), which was treated with the “sanjiao” acupuncture method once a day for six times a week and lasted for 12 weeks, and 30 healthy elderly people were assigned to the normal group (NG), which had no treatment. The distribution of lymphocyte subsets and the levels of some inflammatory cytokines in the peripheral blood of subjects were evaluated using the flow cytometry (FCM) and the enzyme-linked immunosorbent assay (ELISA).Results: A total of 60 subjects were involved in this study, while 58 subjects completed the entire trial. Before treatment, the levels of CD3+ T, CD4+ T cells, CD4+/CD8+, Tregs, B cells, IFN-γ, and IL-10 in patients with VD were significantly decreased compared with the normal group (all P &lt; 0.05 or P &lt; 0.01). The level of TNF-α in peripheral blood of patients with VD was significantly increased (P &lt; 0.01). After acupuncture treatment, the levels of CD3+ T, CD4+ T cells, and IFN-γ were significantly increased (all P &lt; 0.05 or P &lt; 0.01). The level of TNF-α was significantly decreased (P &lt; 0.01). The proportion of Tregs was increased (P &lt; 0.01), but it was still lower than that of the normal group (P &lt; 0.05).Conclusion: The acupuncture method can increase the proportion of CD3+, CD4+ T cells, and Tregs in peripheral blood of patients with VD. And, it reduces the levels of pro-inflammatory factor TNF-α, which achieves the anti-inflammatory effects and immunostimulation. It suggests that acupuncture can improve the peripheral immune dysfunction of patients with VD by regulating the distribution of lymphocyte subsets and the levels of inflammatory cytokines.Clinical Trial Registration: [www.chictr.org.cn], identifier [ChiCTR-IOR-17012052].

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