Biopreservation and Quality Enhancement of Fish Surimi Using Colorant Plant Extracts

The biopreservation, flavoring, and coloration of foodstuffs, e.g., seafoods, with natural plant derivatives are major demands for consumers and overseers. Different colored plant parts, i.e., Hibiscus sabdariffa calyces, Curcuma longa rhizomes, and Rhus coriaria fruits, were extracted and evaluated as biopreservatives, antimicrobial and colorant agents for fish surimi from Oreochromis niloticus. All colorant plant extracts (CPEs) exhibited strong antibacterial activities against screened pathogens, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Pseudomonas aeruginosa. H. sabdariffa extract (HCE) was the most effectual antimicrobial CPEs. S. aureus was the most sensitive strain to CPEs, whereas S. typhimurium and P. aeruginosa were the most resistant strains. The exterior coloration of tilapia surimi with CPEs resulted in great bacterial count reduction in colored products; stored CPEs-colored surimi had enhanced sensorial attributes. HCE-exposed S. aureus indicated bacterial cell lyses in time-dependent manner. CPEs application as colorants and antibacterial and quality enhancing agents is recommended for seafoods’ biopreservation.

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Antibacterial, Antidiabetic and Brine Shrimp Lethality Activities of Some Selected Medicinal Plants from Kavrepalanchok District of Nepal

Journal of Institute of Science and Technology ◽

10.3126/jist.v24i1.24629 ◽

2019 ◽

Vol 24 (1) ◽

pp. 57-62

Author(s):

Khaga Raj Sharma ◽

Rupak Kharel

Keyword(s):

Medicinal Plants ◽

Plant Extracts ◽

Brine Shrimp ◽

Curcuma Longa ◽

Antidiabetic Activity ◽

Diffusion Method ◽

Toxicity Tests ◽

Melia Azedarach ◽

Dependent Manner ◽

Brine Shrimp Lethality

The methanol extracts of nine medicinal plants collected from Kavrepalanchok district of Nepal were subjected to their potential bioactivity viz. antibacterial, antidiabetic and toxicity tests. Antibacterial property of the extracts was evaluated against Gram-positive and Gram-negative bacteria by agar well diffusion method and antidiabetic activity was investigated by α-amylase inhibition assay. The toxicity of plant extracts was assessed by brine shrimp lethality test. All the nine different plant extracts showed antibacterial activity with the zone of inhibition (ZOI) ranging from 5 to 35 mm. Among the studied plant extracts, Psidium guajava showed the highest ZOI against Salmonella typhi (35 mm) while Melia azedarach was most effective against Staphylococcus aureus (22 mm). The percentage of α- amylase enzyme inhibition was found up to 88.56 ± 3.50 in dose dependent manner. The enzyme inhibitory concentration IC50 value of standard, acarbose was 166.01 μg/mL while the most effective anti-diabetic plant extract of Utrica dioica has 186.67 μg/mL. Moreover, various plant extracts depicted various levels of toxic activities; Curcuma longa demonstrated significant toxicity to Artemia salina with LC50 value 62.10 μg/mL, while Agerantina adenophora, P. guajava and M. azedarach showed moderate toxicity with 103.77, 109.37 and 383.58 μg/mL, respectively.

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Antibacterial Action of Curcumin against Staphylococcus aureus: A Brief Review

Journal of Tropical Medicine ◽

10.1155/2016/2853045 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 76

Author(s):

Sin-Yeang Teow ◽

Kitson Liew ◽

Syed A. Ali ◽

Alan Soo-Beng Khoo ◽

Suat-Cheng Peh

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Agents ◽

Curcuma Longa ◽

Antibacterial Activities ◽

Multidrug Resistant ◽

Major Constituent ◽

Global Health Issue ◽

Resistant Strains ◽

Urgent Action

Curcumin, the major constituent of Curcuma longa L. (Zingiberaceae family) or turmeric, commonly used for cooking in Asian cuisine, is known to possess a broad range of pharmacological properties at relatively nontoxic doses. Curcumin is found to be effective against Staphylococcus aureus (S. aureus). As demonstrated by in vitro experiment, curcumin exerts even more potent effects when used in combination with various other antibacterial agents. Hence, curcumin which is a natural product derived from plant is believed to have profound medicinal benefits and could be potentially developed into a naturally derived antibiotic in the future. However, there are several noteworthy challenges in the development of curcumin as a medicine. S. aureus infections, particularly those caused by the multidrug-resistant strains, have emerged as a global health issue and urgent action is needed. This review focuses on the antibacterial activities of curcumin against both methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). We also attempt to highlight the potential challenges in the effort of developing curcumin into a therapeutic antibacterial agent.

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Green Synthesis of Multifunctional Carbon Dots with Antibacterial Activities

Nanomaterials ◽

10.3390/nano11020369 ◽

2021 ◽

Vol 11 (2) ◽

pp. 369 ◽

Cited By ~ 1

Author(s):

Arumugam Saravanan ◽

Moorthy Maruthapandi ◽

Poushali Das ◽

John H. T. Luong ◽

Aharon Gedanken

Keyword(s):

Carbon Dots ◽

Fluorescence Spectra ◽

Antibacterial Effect ◽

Curcuma Longa ◽

Antibacterial Activities ◽

Blue Color ◽

Mean Size ◽

One Step ◽

Bactericidal Activities ◽

Bright Blue

Carbon dots (CDs) were obtained from medicinal turmeric leaves (Curcuma longa) by a facile one-step hydrothermal method and evaluated for their bactericidal activities against two gram-negative; Escherichia coli, Klebsiella pneumoniae, and two gram-positive counterparts; Staphylococcus aureus, S. epidermidis. The CDs exhibited spherical shapes with a mean size of 2.6 nm. The fluorescence spectra of CDs revealed intense fluorescence at λex/em = 362/429 nm with a bright blue color in an aqueous solution. The CDs showed strong photostability under various environmental conditions (pH, salt, and UV-radiation). The complete bactericidal potency of CDs was 0.25 mg/mL for E.coli and S. aureus after 8 h of exposure, while for K. pneumoniae, and S. epidermidis, the CDs at 0.5 mg/mL good antibacterial effect within 8 h and complete eradication after 24 h of exposure is observed. The release of reactive oxygen species played a crucial role in the death of the bacterial cell. The present study provides a strategy for the preparation of CDs from a medicinal plant and their potential antibacterial activities against four common contagious pathogens.

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Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CLPro) Activity

Foods ◽

10.3390/foods10071503 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1503

Author(s):

Carla Guijarro-Real ◽

Mariola Plazas ◽

Adrián Rodríguez-Burruezo ◽

Jaime Prohens ◽

Ana Fita

Keyword(s):

Plant Extracts ◽

Curcuma Longa ◽

Hydrolysis Product ◽

Resonance Energy ◽

Significant Inhibition ◽

Main Role ◽

Turmeric Extract ◽

Ic50 Value

Antiviral treatments inhibiting Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication may represent a strategy complementary to vaccination to fight the ongoing Coronavirus disease 19 (COVID-19) pandemic. Molecules or extracts inhibiting the SARS-CoV-2 chymotripsin-like protease (3CLPro) could contribute to reducing or suppressing SARS-CoV-2 replication. Using a targeted approach, we identified 17 plant products that are included in current and traditional cuisines as promising inhibitors of SARS-CoV-2 3CLPro activity. Methanolic extracts were evaluated in vitro for inhibition of SARS-CoV-2 3CLPro activity using a quenched fluorescence resonance energy transfer (FRET) assay. Extracts from turmeric (Curcuma longa) rhizomes, mustard (Brassica nigra) seeds, and wall rocket (Diplotaxis erucoides subsp. erucoides) at 500 µg mL−1 displayed significant inhibition of the 3CLPro activity, resulting in residual protease activities of 0.0%, 9.4%, and 14.9%, respectively. Using different extract concentrations, an IC50 value of 15.74 µg mL−1 was calculated for turmeric extract. Commercial curcumin inhibited the 3CLPro activity, but did not fully account for the inhibitory effect of turmeric rhizomes extracts, suggesting that other components of the turmeric extract must also play a main role in inhibiting the 3CLPro activity. Sinigrin, a major glucosinolate present in mustard seeds and wall rocket, did not have relevant 3CLPro inhibitory activity; however, its hydrolysis product allyl isothiocyanate had an IC50 value of 41.43 µg mL−1. The current study identifies plant extracts and molecules that can be of interest in the search for treatments against COVID-19, acting as a basis for future chemical, in vivo, and clinical trials.

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Botanicals to Control Soft Rot Bacteria of Potato

The Scientific World JOURNAL ◽

10.1100/2012/796472 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

M. M. Rahman ◽

A. A. Khan ◽

M. E. Ali ◽

I. H. Mian ◽

A. M. Akanda ◽

...

Keyword(s):

Plant Extracts ◽

Curcuma Longa ◽

Soft Rot ◽

Storage Condition ◽

Storage Conditions ◽

Leaf Extracts ◽

Corchorus Capsularis ◽

Rot Disease ◽

And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

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Screening of Hibiscus and Cinnamomum Plants and Identification of Major Phytometabolites in Potential Plant Extracts Responsible for Apoptosis Induction in Skin Melanoma and Lung Adenocarcinoma Cells

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.779393 ◽

2021 ◽

Vol 9 ◽

Author(s):

Neha Kaushik ◽

Hyunji Oh ◽

Yeasol Lim ◽

Nagendra Kumar Kaushik ◽

Linh Nhat Nguyen ◽

...

Keyword(s):

Lung Adenocarcinoma ◽

Plant Extracts ◽

A549 Cells ◽

Skin Melanoma ◽

Plant Parts ◽

Cancer Cell Death ◽

Clinical Drug Development ◽

Targeting Delivery ◽

Site Targeting ◽

Ethanol Extracts

Carcinogenesis is a major concern that severely affects the human population. Owing to persistent demand for novel therapies to treat and prohibit this lethal disease, research interest among scientists is drawing its huge focus toward natural products, as they have minimum toxicity comparable with existing treatment methods. The plants produce secondary metabolites, which are known to have the anticancer potential for clinical drug development. Furthermore, the use of nanocarriers could boost the solubility and stability of phytocompounds to obtain site-targeting delivery. The identification of potential phytochemicals in natural compounds would be beneficial for the synthesis of biocompatible nanoemulsions. The present study aimed to investigate the potential cytotoxicity of ethanol extracts of Hibiscus syriacus and Cinnamomum loureirii Nees plant parts on human skin melanoma (G361) and lung adenocarcinoma (A549) cells. Importantly, biochemical analysis results showed the presence of high phenol (50–55 µgGAE/mg) and flavonoids [42–45 µg quercetin equivalents (QE)/mg] contents with good antioxidant activity (40–58%) in C. loureirii Nees plants extracts. This plant possesses potent antiproliferative activity (60–90%) on the malignant G361 and A549 and cell lines correlated with the production of nitric oxide. Especially, C. loureirii plant extracts have major metabolites that exhibit cancer cell death associated with cell cycle arrest. These findings support the potential application of Cinnamomum for the development of therapeutic nanoemulsion in future cancer therapy.

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In-vitro Antimicrobial Activities and Phytochemical Screening of Selected Plant Extracts against Some Medically and Agriculturally Important Pathogens

European Journal of Medicinal Plants ◽

10.9734/ejmp/2020/v31i1030293 ◽

2020 ◽

pp. 167-189

Author(s):

Yirgashewa Asfere ◽

Ameha Kebede ◽

Dejene Zinabu

Keyword(s):

Stem Bark ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Plant Parts ◽

Antibacterial And Antifungal Activities ◽

Ruta Chalepensis ◽

Crude Extracts ◽

Growth Inhibitory ◽

Scientific Justification

In Ethiopia, many plants are used for medicinal drive-by old-style naturopaths without any scientific justification for their therapeutic values. The principal aim of this study were to evaluate the in vitro antibacterial and antifungal activities of the leaf, root and stem bark extracts of Rhamnus prinioides (gesho), Justicia schimperiana (sensel) and Ruta chalepensis (Tena adam) against some common pathogenic species of bacteria and fungi. The results of this study shown that the crude extracts of ethanol, methanol, hexane and water crude extracts had antimicrobial activities on most bacterial and fungi species of some solvent extracts. Ethanol and methanol crude extracts had the highest growth inhibitory effects as compared with those of the aqueous and hexane crude extracts. But, the four solvent crude extracts had fewer antimicrobial activities than commercially available drug(chloramphenicol and clotrimazole). Campylobacter jejuni and Staphylococcus aureus were found to be the most vulnerable microbes to the crude ethanol (99.5%). The growth-inhibitory events of the crude extracts were found to be significantly dissimilar for both concentrations (30 and 60 mg/ml) in all plant parts (p < 0.05). In general, this study did not only indicate that the antibacterial activities of R. prinioides (gesho), J. schimperiana (sensel) and R. chalepensis (Tena adam). It also accesses a scientific justification for its old-style use against some diseases.

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Biocidal potential of Three Plant Extracts on Rhizopus stolonifer, Causal Organism of Irish Potato (Solanum tuberosum L.) Tuber Rot

Volume 5 - 2020, Issue 9 - September - International Journal of Innovative Science and Research Technology ◽

10.38124/ijisrt20aug480 ◽

2020 ◽

Vol 5 (8) ◽

pp. 774-779

Author(s):

Chuku, E.C ◽

Njoku, A.A ◽

Nmom, F.W

Keyword(s):

Plant Extracts ◽

Severity Score ◽

Solanum Tuberosum L ◽

Curcuma Longa ◽

Irish Potato ◽

Citrus Limon ◽

Aqueous Extracts ◽

Rhizopus Stolonifer ◽

Causal Organism ◽

Significant Difference

Research study was carried out to assess the biocidal effect of aqueous extracts of Curcuma longa, Zingiber officinale, Citrus limon peel and synthetic fungicide Mancozeb against Rhizopus stolonifer using the poisoned food technique on PDA. Various concentrations (50, 75, and 100%) of extracts from the rhizomes of C. longa, Z. officinale, the peel of C. limon and Mancozeb (0.002%) significantly inhibited the mycelia growth of R. stolonifer after 3 days. Effects of the synthetic fungicide (Mancozeb) comparative to the plant extracts were also determined. Although the extracts showed varying degrees of antifungal efficacy, 100% concentration of Z. officinale (58.96%) proved to be more potent against R. stolonifer than the other plant extracts but was lower and significantly different when compared with Mancozeb (73.31%) at (P≤0.05) after 3 days. Extracts of C. longa and C. limon peel showed a lower inhibition level ranging from 45.01% to 56.98% and 9.57% to 18.73% respectively and were significantly different when compared with Mancozeb at (P≤0.05). Inhibition of fungal growth increased with a corresponding increase in extract concentration and days. The plant leaf extracts effectively inhibited the mycelial growth of pathogen in vitro after 3 days. In vivo study was carried out using spore suspensions of R. stolonifer. Fresh, healthy and surface sterilized Irish potato tubers were inoculated with 6.4 x 104 spores/ml and treated with aqueous extracts of C. longa, Z. officinale and C. limon peel after 24 hours. The result showed that all plant extracts had significant effect on disease severity in tubers inoculated with R. stolonifer. However, 100% concentration of Z. officinale gave the best rot reduction caused by R. stolonifer with severity score of 0.33 but it was not significantly different at (p<0.05) from mancozeb which had a severity score of 0.67. However, they were significantly different at (p<0.05) from the inoculated control (3.33). There were variations in weight loss but no significant difference was observed among the various treatment methods adopted.

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Potential of plant extracts in combination with bacterial antagonist treatment as biocontrol agent of red rot of sugarcane

Canadian Journal of Microbiology ◽

10.1139/w06-126 ◽

2007 ◽

Vol 53 (2) ◽

pp. 196-206 ◽

Cited By ~ 14

Author(s):

V. Jayakumar ◽

R. Bhaskaran ◽

S. Tsushima

Keyword(s):

Plant Extracts ◽

Field Experiments ◽

Curcuma Longa ◽

Saccharum Officinarum ◽

Red Rot ◽

Colletotrichum Falcatum ◽

Similar Response ◽

Red Rot Disease ◽

Leaf Extracts ◽

Rot Disease

Plant extracts and antifungal microorganisms were tested singly and in combination for biocontrol of sugarcane red rot disease ( Colletotrichum falcatum ) using two sugarcane ( Saccharum officinarum L.) cultivars, CoC671 and CoC92061, in pot and field experiments. Leaf extracts of Abrus precatorius and Bassia latifolia and the rhizome extract of Curcuma longa reduced Colletotrichum falcatum mycelial growth by 80%, 58%, and 57%, respectively. Although sugarcane- planting materials (setts) treated individually with either Pseudomonas fluorescens Md1 or A. precatorius in pot experiments had the lowest incidences of red rot, 20.1% and 24.2%, respectively, none of the plant extracts were effective in the field. In contrast, when the two varieties were tested separately in two field locations, the setts treated with A. precatorius in combination with a spray or soil application of P. fluorescens Md1 had the lowest incidence of red rot in both locations, e.g., 3.1% and 3.4% incidence for CoC92061 in one location, and had a similar response to the chemical control. The results suggest the applicability of plant-based extracts for the suppression of sugarcane red rot disease in the field as an environment-friendly tool in combination with antagonists.

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Curcumin Induces Neural Differentiation of Human Pluripotent Embryonal Carcinoma Cells through the Activation of Autophagy

BioMed Research International ◽

10.1155/2019/4378710 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 2

Author(s):

Nudjanad Heebkaew ◽

Narawadee Rujanapun ◽

Phongsakorn Kunhorm ◽

Thiranut Jaroonwitchawan ◽

Nipha Chaicharoenaudomrung ◽

...

Keyword(s):

Neural Differentiation ◽

Embryonal Carcinoma ◽

Curcuma Longa ◽

Neuronal Morphology ◽

Carcinoma Cells ◽

Ros Generation ◽

Dependent Manner ◽

Embryonal Carcinoma Cells ◽

Positive Role ◽

Neural Lineage

Curcumin is a natural polyphenolic compound, isolated from Curcuma longa, and is an important ingredient of Asian foods. Curcumin has revealed its strong activities of anti-inflammatory, antioxidant, and anticancer. The efficient amount of curcumin could induce differentiation of stem cells and promoted the differentiation of glioma-initiating cells; however, the mechanisms underlying neural induction of curcumin have not yet been revealed. In this study, neural-inducing ability of curcumin was explored by using human pluripotent embryonal carcinoma cells, NTERA2 cells. The cells were induced toward neural lineage with curcumin and were compared with a standard neutralizing agent (retinoic acid). It was found that, after 14 days of the induction by curcumin, NTERA2 cells showed neuronal morphology and expressed neural-specific genes, including NeuroD, TUJ1, and PAX6. Importantly, curcumin activated neurogenesis of NTERA2 cells via the activation of autophagy, since autophagy-related genes, such as LC3, LAMP1, and ATG5, were upregulated along with the expression of neural genes. The inhibition of autophagy by chloroquine suppressed both autophagy and neural differentiation, highlighting the positive role of autophagy during neural differentiation. This autophagy-mediated neural differentiation of curcumin was found to be an ROS-dependent manner; curcumin induced ROS generation and suppressed antioxidant gene expression. Altogether, this study proposed the neural-inducing activity of curcumin via the regulation of autophagy within NTERA2 cells and underscored the health beneficial effects of curcumin for neurodegenerative disorders, such as Alzheimer’s disease and Parkinson’s disease.

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