scholarly journals Antimicrobial Activity and Characterization of Pomegranate Peel-Based Carbon Dots

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Waseem Akhtar Qureshi ◽  
B. Vivekanandan ◽  
J. Altrin Jayaprasath ◽  
Daoud Ali ◽  
Saud Alarifi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Carbon Dots ◽  
Diffusion Method ◽  
Moderate Activity ◽  
E Coli ◽  
Analytical Instruments ◽  
Antimicrobial Efficiency ◽  
Microbial Efficiency ◽  
Ft Ir

This investigation reports the use of agrowaste pomegranate peels as an economical source for the production of fluorescent carbon dots (C-dots) and their potential application as an antimicrobial agent. The carbon dots were prepared through low-temperature carbonization at 200°C for 120 min. The obtained C-dots were found to be small in size and exhibited blue luminescence at 350 nm. Further, the synthesized C-dots were characterized with the help of analytical instruments such as DLS, UV-visible, FT-IR, TEM, and fluorescence spectrophotometer. Antimicrobial activity of the C-dot PP was estimated by the agar diffusion method and MIC. S. aureus and K. pneumoniae are showing susceptibility towards C-dot PP when compared to the standard and showing a moderate activity against P. aeruginosa and resistance towards E. coli. The obtained C dot PPs were found to be around 5-9 nm in size confirmed from DLS analysis and supported by TEM. The synthesized C-dots were investigated to understand their microbial efficiency against pathogens and found to have antimicrobial efficiency. These results suggest that pomegranate peels are a potential source of carbon dots with antimicrobial efficiency.

scholarly journals Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities

2021 ◽  
Vol 3 (8) ◽  
Author(s):  
Muhammad Yasir ◽  
Basit Zeshan ◽  
Nur Hardy A. Daud ◽  
Izzah Shahid ◽  
Hafza Khalid
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Activity ◽  
Antifungal Drugs ◽  
Proteinase K ◽  
Diffusion Method ◽  
List Type ◽  
Bacterial Isolates ◽  
E Coli ◽  
Inhibitory Potential

Abstract There is a need for more efficient and eco-friendly approaches to overcome increasing microbial infections. Bacteriocins and chitinases from Bacillus spp. can be powerful alternatives to conventional antibiotics and antifungal drugs, respectively. The purpose of this study was to assess the inhibitory potential of bacteriocins and chitinase enzymes against multiple resistant bacterial and fungal pathogens. Bacterial isolates were selected by growth on minimal salts medium and after that were morphologically and biochemically characterized. The physiochemical characterization of bacteriocins was carried out. The inhibitory potential of bacteriocins towards six pathogenic bacteria was determined by the well diffusion assay while chitinase activity towards three fungal strains was determined by the dual plate culture assay. Two bacterial strains (WW2P1 and WRE4P2), out of nine showed inhibition of K. pneumonia, P. aeruginosa, E. coli and MRSA while WW4P2 was positive against S. typhimurium and E. coli and WRE10P2 against P. aeruginosa, S. pneumoniae. Two bacterial isolates (WW3P1 and WRE10P2) were chosen for further study on the basis of their antifungal activities. Of these, WW3P1 isolate was more effective against A. fumigatus as well as A. niger. The proteinaceous nature of the bacteriocins was confirmed by treatment of the crude extract with proteinase K. It was found that the inhibitory activity of strain WW3P1 against E. coli was highest at 20 °C, and against S. pneumoniae it was at 20 °C and pH 10 after treatment with EDTA. Inhibition by strain the WRE10P2 against P. aeruginosa was highest at 20 °C and pH 14. It was found that EDTA increased the inhibitory activity of strain WW2P1 against P. aeruginosa, K. pneumoniae and E. coli by 2 ± 0.235, 3.5 ± 0.288, 2.5 ± 1.040 times, respectively, of strain WRE4P2 against P. aeruginosa and E. coli by 2.5 ± 0.763, 2.7 ± 0.5 times, respectively, and of strain WRE10P2 against S. pneumoniae by 3 ± 0.6236 times. The isolates have promising inhibitory activity, which should be further analyzed for the commercial production of antimicrobials. Article highlights The current study aimed to isolate the microbiome from wheat plant (Triticum aestivum L.), to screen for bacteriocin production and to assess its antimicrobial activity against human pathogens. Forty-one phenotypically different bacterial colonies were subjected to bacteriocin purification from which 25 colonies showed positive reactions. These 25 bacterial isolates were screened against six different human bacterial pathogens using the well diffusion method to check the antimicrobial activity. Out of nine bacterial isolates, WW3P1 and WRE10P2 were able to degrade the chitin and utilize it as their sole energy source. Strain WRE4P2 exhibited partial inactivation in its activity against MRSA after treatment with proteinase K.

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

scholarly journals CHARACTERIZATION OF LACTIC ACID BACTERIA AND ANTIMICROBIAL ACTIVITY IN SUI WU’U FROM BAJAWA DISTRICT, NUSA TENGGARA TIMUR, INDONESIA

2020 ◽  
pp. 44-49
Author(s):  
ROSALINA YULIANA AYEN ◽  
ENDANG KUSDIYANTINI ◽  
SRI PUJIYANTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
16S Rrna ◽  
Diffusion Method ◽  
E Coli ◽  
Query Cover

Objective: This research aimed to isolate, determine the characteristics of lactic acid bacteria (LAB) of Sui Wu’u from Bajawa, Nusa Tenggara Timur and identify LAB using 16S rRNA potential as antimicrobial activity against pathogenic bacteria. Methods: Sui Wu’u which has been stored for 6 months was obtained from Bajawa district, inoculated on de Man Rogosa-Sharpe Agar (Merck) + 0.5% CaCO3, purification of LAB, characterization of selected isolates, biochemical test, tolerance test for pH, viability to test temperature, and content NaCl, determination of antimicrobial action by the agar well disk diffusion method using antibiotic (Amoxicillin) as a control and as indicator bacteria (Staphylococcus aureus and Escherichia coli) and isolation of genomic 16S rRNA; molecular identification. Results: Based on research results obtained five isolates of LAB, Gram staining the LAB isolated from Sui Wu’u showed that the isolated bacteria (bacilli and coccus) are Gram-positive, catalase-negative and the isolates have tolerance of viability at temperatures of 10°C, 45°C, and 50°C and to salinitas of 4% and 6.5%. The inhibitory zone LAB isolates (2PKT) against E. coli bacteria (20 mm) and S. aureus (12 mm), and (2PKB) against E. coli bacteria (17 mm) and S. aureus (10 mm). The two selected isolates were identified as Lactobacillus fermentum strain HB bacteria with 100% identification value and 98.93% query cover and L. fermentum strain HT with 100% identification value and 99.23% query cover. Conclusion: L. fermentum from Sui Wu’u has antibacterial activity against Staphylococcus aureus and Escherichia coli.

scholarly journals SYNTHESIS, CHARACTERIZATION OF ANTIMICROBIAL ACTIVITY OF 22’DICHLOROHYDROBENZOIN

2018 ◽  
Vol 11 (12) ◽  
pp. 334
Author(s):  
Thanuja B ◽  
Charles Kanagam
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Optical Behavior ◽  
Thermal Stability Of

Objective: The objective of this work to evaluate the antimicrobial activities of synthesized 22’dichlorohydrobenzoin (22’CD) a new organic crystal.Methods: 22’CD a new organic crystal was grown by vapor diffusion method. Single crystals of 22’CD have been subjected to X-ray diffraction analysis to estimate the lattice parameters and the space group. The molecular structure was confirmed using Fourier transform infrared and nuclear magnetic resonance (NMR) spectral analyses. Optical behavior and thermal stability of the crystal were determined using UV-Vis spectroscopy and thermogravimetry-differential thermal analysis curves. In the present study, antimicrobial activity of 22’CD was evaluated against Escherichia coli and Bacillus subtilis was evaluated by agar well diffusion method.Results: Antibacterial activity of 22’CD was analyzed with ciprofloxacin and miconazole standard and tested against E. coli, Pseudomonas aeruginosa, Salmonella paratyphi, Klebsiella pneumonia’s, Staphylococcus aureus, Streptococcus progenies, and B. subtilis.Conclusion: The 22’CD was found to be effective against E. coli and B. subtitles.

scholarly journals Polyphenolics profile effects upon the antioxidant and antimicrobial activity of propolis extracts

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mădălina Maria Nichitoi ◽  
Ana Maria Josceanu ◽  
Raluca Daniela Isopescu ◽  
Gabriela Olimpia Isopencu ◽  
Elisabeta-Irina Geana ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Diffusion Method ◽  
Type Model ◽  
E Coli ◽  
Antimicrobial Efficiency ◽  
Antioxidant And Antimicrobial Activity ◽  
Dpph Scavenging ◽  
Antibacterial And Antifungal ◽  
The Relationship

AbstractPropolis, a complex bee product, is a source of numerous bioactive principles, beneficial for human health, therefore it is intensively studied. In the present work, extracts of propolis from Bihor Romanian County were studied to identify the relationship between the polyphenolic derivatives profile and their antioxidant and antimicrobial activity. Extracts were obtained using water and 25%, 50%, and 70% ethanolic solutions (w/w), at 2:1, 4:1, and 6:1 liquid: solid ratios (w/w). 21 polyphenolic derivatives were quantified by UHPLC-MS, proving that the extracts composition strongly depends on the solvent. The sum of quantified polyphenolics extracted varied between 1.5 and 91.2 mg/g propolis. The antioxidant capacity was evaluated using the free radicals 2,2’-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging methods. Antimicrobial efficiency was tested against Gram-positive (B. subtilis), Gram-negative bacteria (E. coli), and fungi (C. albicans) by disc-diffusion method. All extracts, even the aqueous ones, demonstrated antibacterial and antifungal activity. Chemometric methods (partial least squares) and a saturation-type model were used to evaluate the contribution of various bioactive principles in building the antioxidant capacity of extracts. Both experimental and modelling results show that 50% ethanolic extracts provide a rich polyphenolics profile and ensure a good antioxidant capacity.

scholarly journals Antimicrobial Activity of Thin Solid Films of Silver Doped Hydroxyapatite Prepared by Sol-Gel Method

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Simona Liliana Iconaru ◽  
Patrick Chapon ◽  
Philippe Le Coustumer ◽  
Daniela Predoi
Keyword(s):  
Thin Films ◽  
Antimicrobial Activity ◽  
Sol Gel ◽  
Optical Emission ◽  
Antimicrobial Effect ◽  
Gel Method ◽  
Sol Gel Method ◽  
E Coli ◽  
Ft Ir

In this work, the preparation and characterization of silver doped hydroxyapatite thin films were reported and their antimicrobial activity was characterized. Silver doped hydroxyapatite (Ag:HAp) thin films coatings substrate was prepared on commercially pure Si disks by sol-gel method. The silver doped hydroxyapatite thin films were characterized by various techniques such as Scanning electron microscopy (SEM) with energy Dispersive X-ray attachment (X-EDS), Fourier transform infrared spectroscopy (FT-IR), and glow discharge optical emission spectroscopy (GDOES). These techniques have permitted the structural and chemical characterisation of the silver doped hydroxyapatite thin films. The antimicrobial effect of the Ag:HAp thin films onEscherichia coliandStaphylococcus aureusbacteria was then investigated. This is the first study on the antimicrobial effect of Ag:HAp thin films obtained by sol-gel method. The results of this study have shown that the Ag:HAp thin films withxAg=0.5are effective againstE. coliandS. aureusafter 24 h.

scholarly journals Synthesis, Characterization and Antimicrobial Activity of N-2-(4-Chlorophenyl)acetyl Derivatives of (S)-Amino Acids

2019 ◽  
Vol 32 (2) ◽  
pp. 381-384
Author(s):  
G. Venkateshappa ◽  
P. Raghavendra Kumar ◽  
Krishna2
Keyword(s):  
Amino Acids ◽  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative ◽  
E Coli ◽  
Ft Ir ◽  
Bacteria And Fungi ◽  
Derivatives Of ◽  
Ft Ir Spectroscopy ◽  
Agar Well Diffusion Method

This paper reports the synthesis, characterization and antibacterial activity of N-2-(4-chlorophenyl)acetyl derivatives of various (S)-amino acids such as (S)-alanine, (S)-phenylalanine, (S)-leucine, (S)-methionine, (S)-proline and (S)-tryptophane. These compounds have been successfully synthesized and their structures were confirmed by 1H NMR and 13C NMR and FT-IR spectroscopy. The antimicrobial activity of these six (S)-amino acids derivatives have been evaluated by the agar well diffusion method against pathogens both Gram-positive (S. aureus) and Gram-negative (K. aerogenes, E. coli and P. desmolyticumas) bacteria and fungi (A. flavus and C. albicans). All these compounds have shown mild to moderate antimicrobial activity.

scholarly journals Novel Triazine Centred Manganese Based Complex: A Photophysical and Biological Studies

2019 ◽  
Vol 32 (1) ◽  
pp. 91-94
Author(s):  
L. Alphonse ◽  
P. Tharmaraj ◽  
B. Avila Josephine ◽  
V. Mary Teresita
Keyword(s):  
Antimicrobial Activity ◽  
Metal Complex ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Fluorescent Properties ◽  
E Coli ◽  
Physico Chemical ◽  
Biological Studies ◽  
Ft Ir

In this work, Mn(II) complex of triazine based ligand has been synthesized and characterized using various physico-chemical methods including C,H,N elemental analysis, FT-IR, 1H NMR and EI-mass analysis. The synthesized compounds serve as potential photoactive material as indicated from its characteristic fluorescent properties. The ligand and its metal complex were assayed for in vitro antimicrobial activity on four bacterial strains (S. aureus, B. subtilis, E. coli and K. pneumoniae) using well-diffusion method and it was observed that metal complex showed enhanced antimicrobial activity against all tested strains as compared to ligand.

scholarly journals Synthesis and antimicrobial activity of some new pyrazoline derivatives bearing sulfanilamido moiety

2019 ◽  
Vol 10 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Maysoon Mohammed Almahdi ◽  
Ahmed Elsadig Mohammed Saeed ◽  
Nadia Hanafy Metwally
Keyword(s):  
Antimicrobial Activity ◽  
Moderate Activity ◽  
Gram Positive ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli ◽  
Chemical Structures ◽  
In Vitro Antibacterial Activity ◽  
Ft Ir

In the present study, a series of new pyrazoline derivatives bearing sulfanilamido moiety were synthesized and obtained in good yields. The chemical structures of the compounds were elucidated by spectral data (FT-IR, MS, UV-VIS and NMR). The synthesized compounds 41-70 were screened for their antimicrobial activity and compared with controls. The in vitro antibacterial activity of compounds 41-45 and 48-57 was checked against two Gram positive microorganisms (S. aureus and S. mutans) and three Gram negative microorganisms (E. coli, K. pneumonia and P. aureginosa), their antifungal activity was checked against C. albicans. The preliminary results showed that these compounds had moderate activity against the tested organisms. Compounds 41, 48, 51 and 56 exhibited promising antimicrobial activity against S. aureus compared to standard drug Ampicilin. Final synthesized compounds 58-70 were tested against two Gram positive (S. aureus and B. subtilis) and two Gram negative (E. coli and P. aureginosa) microorganisms, their activity against C. albicans was also checked and they did not exhibit any antimicrobial activity.

Synthesis, SAR, In-Silico appraisal and Anti-Microbial Study of substituted 2-aminobenzothiazoles derivatives

2019 ◽  
Vol 15 ◽  
Author(s):  
Devidas G. Anuse ◽  
Suraj N. Mali ◽  
Bapu R. Thorat ◽  
Ramesh S. Yamgar ◽  
Hemchandra K. Chaudhari
Keyword(s):  
Antimicrobial Activity ◽  
In Silico ◽  
Docking Study ◽  
Moderate Activity ◽  
Molecular Docking Study ◽  
Mass Spectral ◽  
Gram Positive Bacteria ◽  
Ft Ir ◽  
In Silico Molecular Docking

Background: Antimicrobial resistance is major global health problem, which is being rapidly deteriorating the quality of human health. Series of substituted N-(benzo[d]thiazol-2-yl)-2-(4-(6-fluorobenzo[d]isoxazol-3-yl)piperidin-1-yl)acetamide (3a-j) were synthesized from substituted N-(benzo[d]thiazol-2-yl)-2-chloroacetamide/bromopropanamide (2a-j) and 6-fluoro-3-(piperidin-4-yl)benzo[d]isoxazole (2) and further evaluated for their docking properties and antimicrobial activity. Methods: All synthesized compounds were characterized by FT-IR, NMR and Mass spectral analysis. All compounds were allowed to dock against different antimicrobial targets having PDB ID: 1D7U and against common antifungal target having PDB ID: 1EA1. Results: The compounds 3d and 3h were showed good activity against Methicillin-resistant Staphylococcus aureus (MRSA, resistance Gram-positive bacteria). All synthesized compounds showed good to moderate activity against selected bacterial and fungal microbial strains. If we compared the actual in-vitro antimicrobial activity and in-silico molecular docking study, we found that molecules 3i and 3h were more potent than the others. Conclusion: Our current study would definitely pave the new way towards designing and synthesis of more potent 2-aminobenzothiazoles derivatives.

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