scholarly journals Assessment of Wettability and Contact Angle of Bonding Agent with Enamel Surface Etched by Five Commercially Available Etchants: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Deepika Katyal ◽  
Aravind Kumar Subramanian ◽  
Adith Venugopal ◽  
Anand Marya
Keyword(s):  
Contact Angle ◽  
Statistical Analysis ◽  
In Vitro Study ◽  
Enamel Surface ◽  
Bonding Agent ◽  
Etch Pattern ◽  
Angle Measurements ◽  
Significant Difference ◽  
Contact Angle Measurements

Background. The quantity and quality of the etching pattern produced by acids play a significant role in the wettability and contact angle of the adhesive with the enamel surface in orthodontics. The better the etch pattern, the more the surface energy of the enamel, better the penetration of the adhesive, which ultimately results in better bond strength. The present study aimed to evaluate the contact angle of the bonding agent with the enamel surface etched by five commercially available etchants and check if any difference existed between the five. Materials and Methods. Twenty-five human maxillary and mandibular central incisors and premolars extracted for orthodontic or dental purposes were used in this study as samples. The teeth were allocated into five groups based upon the etchant used to etch the enamel surface. After the samples were etched, a hard tissue microtome was used to create thin slices of the enamel surface. The samples were then exposed to bonding agent Ormco Enlight. The bonding agent was released in the form of droplets onto the enamel slices mounted on a contact angle goniometer. The contact angle values were tabulated, and statistical analysis using the one-way ANOVA test was carried out. Results. The contact angle measurements of the etchant group, DPI, were the lowest, while D-tech has the highest contact angle values. However, statistical analysis revealed no statistically significant difference between the contact angle measurements of the five groups included in the study. Conclusion. No statistically significant difference existed between the five etchant groups included in the study. However, commercially available etchant DPI showed lesser contact angle and thus better wettability in comparison to other groups. Further elemental analysis and surface analysis are required to validate these results.

Surface Wettability Enhances Osteoblast Adhesion on Silicon-Substituted Hydroxyapatite Thin Films

2007 ◽  
Vol 330-332 ◽  
pp. 877-880 ◽  
Author(s):  
E.S. Thian ◽  
J. Huang ◽  
Serena Best ◽  
Zoe H. Barber ◽  
William Bonfield
Keyword(s):  
Thin Films ◽  
Contact Angle ◽  
Actin Filaments ◽  
Culture Study ◽  
Cell Culture Study ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Osteoblast Adhesion ◽  
Lower Contact

Crystalline hydroxyapatite (HA) and 0.8 wt.% silicon-substituted HA (SiHA) thin films were produced using magnetron co-sputtering. These films were subjected to contact angle measurements and in vitro cell culture study using human osteoblast-like (HOB) cells. A wettability study showed that SiHA has a lower contact angle, and thus is more hydrophilic in nature, as compared to HA. Consequently, enhanced cell growth was observed on SiHA at all time-points. Furthermore, distinct and well-developed actin filaments could be seen within HOB cells on SiHA. Thus, this work demonstrated that the surface properties of the coating may be modified by the substitution of Si into the HA structure.

scholarly journals Milled versus moulded mock-ups based on the superimposition of 3D meshes from digital oral impressions: a comparative in vitro study in the aesthetic area

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Francesca Cattoni ◽  
Giulia Teté ◽  
Alessandro Mauro Calloni ◽  
Fabio Manazza ◽  
Giorgio Gastaldi ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Test Group ◽  
In Vitro Study ◽  
Mean Value ◽  
Digital Impression ◽  
Control Group ◽  
Minimal Invasiveness ◽  
Significant Difference ◽  
The Aesthetic

Abstract Background Aesthetic porcelain veneers proved to be a long-term reliable prosthetic solution, ensuring minimal invasiveness. The use of veneers requires an adhesive cementation technique, so maintaining as much enamel as possible is to ensure lasting success. A diagnostic mock-up is a key tool that allows a preview of the outcome of the aesthetic restoration: it is obtainable both in an analog and digital way. With the recent developments in impression technology and the ever so fast growing use of CAD-CAM technologies it is useful to understand the pros and cons of either one of these techniques (analog and digital) in order to identify the easier and more convenient workflow in aesthetic dentistry. Methods After taking pictures and impressions of the dental arcs of a patient in need of aesthetic rehabilitation, 52 resin models were produced and a digital drawing of the smile was outlined. Both an analog and a digital wax-up were obtained from two of the 52 models: the latter was obtained using digital impressions and a dedicated software. The analog wax-up was then used to produce 25 matrices that have later been used to mould 25 resin mock-ups using a traditional moulding protocol (Control Group - CG). The digital wax-up was used to mill 25 PMMA mock-ups. Each mock-up, both milled and moulded (total 50), was then laid on the other 50 resin models as a digital impression of it was taken. The STL. files of the milled mock-ups were compared with the 3D CAD wax-up made using a specific software. The STL. files of the analog printed mock-ups were compared with the traditional wax-up design. A statistical analysis was carried out to evaluate the difference between the groups. Results The statistical analysis showed a significant difference (P > 0.01) between the mean value of the distance between the points of the overlapping STL. meshes in GC (0.0468 mm) and in TG (Test Group - TG) (0,0109 mm). Conclusions The study showed a difference in accuracy between traditional moulded and milled mock-ups compared to their original wax-up. The data analysis reports that the digital method allows for greater accuracy. Within the limitations of this study, a fully digital workflow is to considered more reliable when it come to creating an esthetic mockup: the digital procedure has been shown to be more accurate than the one made manually which is much more operator dependent and it brings an increase to the chance of error, and that could ultimately affect the final result.

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

Effect of Bonding Agent on Retention of Different Sealants: An in Vitro Study

2021 ◽  
Vol 45 (3) ◽  
pp. 177-185
Author(s):  
Moaz H Attar ◽  
Medhat A Abdallah ◽  
Hussein A Alharthy ◽  
Omar A El Meligy
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Bonding Agent ◽  
Micro Ct ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Sealant Retention

Objectives: To investigate the effect of Co-curing versus Staged-curing and No-bonding on retention of different resin-based sealants (RBS). Study design: For shear bond strength (SBS) and microleakage tests, 90 extracted premolars were divided equally into 3 groups (I, II, III). Each group was further subdivided equally into 3 subgroups (a, b, c). No-bonding subgroups did not receive a bonding agent, Staged-curing subgroups received a bonding agent that was cured before sealant application, while Co-curing subgroups received a bonding agent that was cured after sealant application. Seal-it was applied for group I, Helioseal-F for group II and Clinpro for group III. SBS buttons were tested using Instron machine, while microleakage specimens were examined using micro-CT. Results: Clinpro showed the highest SBS values in Staged-curing and No-bonding groups (8.72±2.39, 12.51±3.16) respectively. Staged-curing was significantly greater in SBS values than those for other groups (P<0.05). There was a significant difference in microleakage values of Staged-curing among different RBS (P = 0.003), while there was no significant difference in values of No-bonding and Co-curing among different RBS (P = 0.541, P = 0.521). Conclusions: The use of a bonding agent as Staged-curing was more effective in improving sealant retention than No-bonding and Co-curing.

scholarly journals Effects of CO2 laser irradiation on matrix-rich biofilm development formation – An in vitro study

2016 ◽  
Author(s):  
Bruna Raquel Zancope ◽  
Vanessa B Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte, Jr ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Laser Irradiation ◽  
Co2 Laser ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

Background. CO2 laser has been used to morphologically and chemically modify the dental enamel surface as well as to turn it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of CO2 laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2 laser (λ = 10.6 µM). Methods. For this in vitro study, it was employed 96 specimens of bovine enamel, which were divided into 2 groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by 1, 3 and 5 days under intermittent cariogenic condition in the irradiated and non irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and in fifth day, were evaluated by polysaccharides analysis, quantitative real time PCR as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test. Results. The mean of log CFU/ml obtained for the 1-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to CO2 laser, CFU/mL and CFU/ Dry Weight in 3 day was reduced significantly compared with control group. The difference in the genes expression (gtfB and gbpB) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with CO2 laser. Similar morphology was also visible with both treatments, however irradiated group revealed evidence of melting and fusion in the specimens. Conclusion. In conclusion CO2 laser irradiation modify the energy surface and disrupt the initial biofilm formation.

scholarly journals Development of New Method to Reposition Orthodontic Brackets Without Debonding by Using an Ultrasonic Device: An In Vitro Study

2020 ◽  
Vol 54 (3) ◽  
pp. 214-219
Author(s):  
Toshihito Sato ◽  
Michiru Wakabayashi
Keyword(s):  
In Vitro Study ◽  
Enamel Surface ◽  
New Method ◽  
Frictional Heat ◽  
Air Cooling ◽  
Ultrasonic Device ◽  
Significant Difference ◽  
Time Required ◽  
Bracket Position

Objective: The purpose of this study was to develop a new method to change a bracket position without debonding the metal bracket that was bonded with a polymethyl methacrylate (PMMA) orthodontic adhesive resin by using an ultrasonic device. Materials and Methods: An originally designed scaler tip was developed for a piezoelectric ultrasonic scaler. The adhesive turned to melting state by thermal heat that was generated by ultrasonic frictional heat. When the adhesive was in melting state, the bracket was rotated approximately 20° and held in this position until the adhesive hardened again by air cooling. Shear bond strengths were measured on each sample in the control and the repositioning groups. To evaluate the thermal effects of the increased temperature on the enamel surface and pulpal cavity, extracted human lower premolars were monitored during the repositioning time. Results: The time required for the repositioning procedures was approximately 7 s. The temperature of the enamel surface increased by 53°C and the pulpal wall increased by 0.5°C during the repositioning procedures. The bonding strength showed no significant difference between the control and repositioning groups. Conclusion: This method for bracket repositioning without debonding using an ultrasonic device proved to be a new method for bracket repositioning.

scholarly journals Evaluation of Enamel Surface after Removal of Adhesive Resin: An in Vitro Study

2018 ◽  
Vol 8 (2) ◽  
pp. 12-16
Author(s):  
Geeta Pyakurel ◽  
Gazi Shamim Hassan ◽  
Mahmood Sajedeen
Keyword(s):  
Surface Roughness ◽  
Stainless Steel ◽  
Tungsten Carbide ◽  
In Vitro Study ◽  
T Test ◽  
Enamel Surface ◽  
Adhesive Resin ◽  
Significant Difference ◽  
And Tungsten

Introduction: Preservation of the enamel surface during the removal of orthodontic appliance is an essential aspect for clinicians. However, various therapeutic measures can affect the enamel surface. The objective of the research is to evaluate the roughness of enamel surface after the removal of adhesive resin. Materials & Method: 40 extracted human premolar teeth were randomly divided into two groups and the enamel surfaces were initially subjected to profilometer for the assessment of surface roughness. Following bracket bonding, debonding was done and adhesive resin was removed by stainless steel bur in Group A and tungsten carbide bur in Group B. Again, the surface roughness was measured by profilometer. Independent t-test was performed to compare the enamel surface roughness between two groups and paired t-test to compare the enamel surface roughness within the groups. Result: The mean average surface roughness of stainless steel bur was 27.009 ± 4.8420 μm and tungsten carbide was 31.426 ± 5.0956 μm. The result showed that there was significant difference in enamel surface roughness between two groups. Conclusion: The roughness values were found to be significantly decreased with the use of stainless steel bur (SS) than tungsten carbide bur (TC).

scholarly journals Mathematical Model to Predict the Affinity Between Aggregate/Bitumen

2020 ◽  
Vol 22 (3) ◽  
pp. 197
Author(s):  
P. Caputo ◽  
G.A. Ranieri ◽  
D. Miriello ◽  
A. Bloise ◽  
A.A. Abe ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Contact Angle ◽  
Chemical Composition ◽  
Bond Angle ◽  
Angle Measurements ◽  
Significant Difference ◽  
Road Surfaces ◽  
Contact Angle Measurements ◽  
Established Fact ◽  
The Mathematical Model

The stones used for the construction of road surfaces have a complex mineralogical and hence chemical composition. They are made up of several types of minerals put together. This generates a significant difference in adhesion with the bituminous binder. The aim of this study is to create a mathematical model able to predict the adhesion between bitumen and stone on the basis of contact angle measurements made on different pure minerals. The mathematical model used was developed keeping in mind the exponential bond that the minerals have with the corresponding bond angle. This model also confirmed the established fact that the lower the value of Δ, the better the adhesion between the bitumen and the aggregate.

scholarly journals THE EFFECT OF THERMOCYCLING ON MARGINAL SEALING ABILITY OF MTA PLUS AND BIODENTINE WHEN USED AS ROOT-END FILLING MATERIALS

2020 ◽  
Vol 8 (11) ◽  
pp. 894-900
Author(s):  
Deebah Choudhary ◽  
Keyword(s):  
Statistical Analysis ◽  
In Vitro Study ◽  
Blue Dye ◽  
Depth Of Penetration ◽  
Jammu And Kashmir ◽  
Filling Materials ◽  
Sealing Ability ◽  
Group I ◽  
Significant Difference

Background:Marginal microleakage occurs when restorations are poorly adapted to the cavity walls which leads to penetration of bacteria, fluid and nutrient into the marginal gap. Aim: The study aimed to evaluate the effect of thermocycling on marginal sealing ability of two root-end filling materials i.e. MTA Plus and Biodentine. Materials and Methods: An in vitro study was conducted at Department of Conservative Dentistry and Endodontics in Institute of Dental Sciences (IDS), Jammu, Jammu and Kashmir, India. Forty extracted teeth with single root were decoronated maintaining a standardized length of 16mm. Biomechanical preparation and obturation was done for all the teeth. Samples were stored at 37oC and 100% humidity for seven days. Apical 3mm was resected and cavities were prepared which were randomly divided into two experimental groups with twenty samples each. The cavities were filled with MTA Plus in group I and Biodentine in group II. These groups were further divided into two subgroups of ten each (n=10). The second subgroup of both the groups did not receive any treatment and the first subgroup samples were subjected to 500 thermocycles in water bath at temperature ranges between 5oC and 55oC with a dwell time of 30 second. The samples were immersed in methylene blue dye and after through washing they were sectioned longitudinally. Examination was done under stereomicroscope to measure the depth of penetration. Statistical Analysis: The data collected were put to statistical analysis by one-way ANOVA (analysis of variance) and post hoc tests. Results: The average depth of dye penetration was least for Biodentine when thermocycling treatment was not given (0.13mm) and maximum value was depicted by MTA Plus when undergone thermal cycles (0.57mm). It is also noted that no significant difference is seen in Biodentine with or without artificial aging procedure. Conclusion:Biodentine showed least amount of microleakage, thus a better sealing ability.Alsothere was little effect of thermocycling onmicroleakage of both the materials.

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