scholarly journals Molecular Blood Group Screening in Donors from Arabian Countries and Iran Using High-Throughput MALDI-TOF Mass Spectrometry and PCR-SSP

2020 ◽  
Vol 47 (5) ◽  
pp. 396-408
Author(s):  
Brigitte Katharina Flesch ◽  
Vanessa Scherer ◽  
Burkhard Just ◽  
Andreas Opitz ◽  
Oswin Ochmann ◽  
...  
Keyword(s):  
Blood Group ◽  
Arabian Peninsula ◽  
Blood Groups ◽  
Heterozygous Mutation ◽  
African Countries ◽  
Maldi Tof ◽  
Panel Methods ◽  
Variant Alleles ◽  
Compatible Blood ◽  
Group Allele

Background and Aims: Only little is known about blood groups other than ABO blood groups and Rhesus factors in Arabian countries and Iran. During the last years, increased migration to Central Europe has put a focus on the question how to guarantee blood supply for patients from these countries, particularly because hemoglobinopathies with the need of regular blood support are more frequent in patients from that region. Therefore, blood group allele frequencies should be determined in individuals from Arabian countries and Iran by molecular typing and compared to a German rare donor panel. Methods: 1,111 samples including 800 individuals from Syria, 147 from Iran, 123 from the Arabian Peninsula, and 41 from Northern African countries were included in a MALDI-TOF MS assay to detect polymorphisms coding for Kk, Fy(a/b), Fynull, Cw, Jk(a/b), Jo(a+/a–), Lu(a/b), Lu(8/14), Ss, Do(a/b), Co(a/b), In(a/b), Js(a/b), Kp(a/b), and variant alleles RHCE*c.697C>G and RHCE*c.733C>G. Yt(a/b), S–s–U–, Velnull, Conull, and RHCE*c.667G>T were tested by PCR-SSP. Results: Of the Arabian donors, 2% were homozygous for the FY*02.01N allele (Fynull), and 15.7% carried the heterozygous mutation. However, 0.8% of the German donors also carried 1 copy of the allele. 3.6% of all and 29.3% of Northern African donors were heterozygous for the RHCE*c.733C>G substitution, 0.4% of the Syrian probands were heterozygous for DO*01/DO*01.-05, a genotype that was lacking in German donors. Whereas the KEL*02.06 allele coding for the Js(a) phenotype was missing in Germans; 0.8% of the Syrian donors carried 1 copy of this allele. 1.8% of the Syrian but only 0.3% of the German donors were negative for YT*01. One donor from Northern Africa homo­zygously carried the GYPB*270+5g>tmutation, inducing the S–s–U+w phenotype, and in 2 German donors a GYPB*c.161G>A exchange, which induces the Mit+ phenotype, caused a GYPB*03 allele dropout in the MALDI assay. The overall failure rate of the Arabian panel was 0.4%. Conclusions: Some blood group alleles that are largely lacking in Europeans but had been described in African individuals are present in Arabian populations at a somewhat lower frequency. In single cases, it could be challenging to provide immunized Arabian patients with compatible blood.

scholarly journals The Transfusion Behavior of Avian Erythrocytes: The Lack of Functional Transplantation Antigens in a Nucleated Cell

Blood ◽  
1961 ◽  
Vol 18 (2) ◽  
pp. 207-219 ◽  
Author(s):  
ROBERT SILBER ◽  
STEPHEN E. HEDBERG ◽  
JOSEPH H. AKEROYD ◽  
DONALD FELDMAN
Keyword(s):  
Blood Group ◽  
Blood Groups ◽  
Naturally Occurring ◽  
Apparent Relationship ◽  
Avian Erythrocytes ◽  
Avian Erythrocyte ◽  
Compatible Blood ◽  
Transplantation Antigens

Abstract 1. Autotransfusion and cross-transfusion experiments in the turkey reveal that the avian erythrocyte, despite the presence of a nucleus, survives normally in homologous recipients of compatible blood groups. 2. Skin homografts were rejected without apparent relationship to blood group compatibility. 3. Evidence for blood groups in the turkey is presented. While no naturally occurring iso-agglutinins were found, stimulation led to the appearance of incomplete antibodies.

Blood Group Genotyping to Prevent Alloimmunization in Children with Sickle Cell Disease

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 2486-2486
Author(s):  
Nancy Robitaille ◽  
Yves D. Pastore ◽  
Anne-Julie Landry ◽  
Catherine Latour ◽  
Josée Lavoie ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Blood Group ◽  
Blood Donors ◽  
Conflicts Of Interest ◽  
African Descent ◽  
African Ancestry ◽  
Adult Life ◽  
Blood Groups ◽  
Cell Disease ◽  
Compatible Blood

Abstract Introduction:Sickle cell disease (SCD) patients can suffer from devastating complications of their disease as a result of chronic hemolysis and vasculopathy. Chronic blood transfusion can effectively prevent some of the most severe organ damage such as cerebral vasculopathy. However, alloimmunization remains a major concern for chronically transfused patients, even if prophylactic antigen matching is performed for C, E and Kell antigens. Variants in the Rh blood group have been well described in people of African descent and must be considered when transfusing patients with SCD. The main objectives of this study were to determine the frequency of variants in the Rh and Duffy blood groups and to identify compatible blood donors presenting similar Rh variants Methods: Extended erythrocyte phenotypes were routinely done at diagnosis for every SCD patient followed at the SCD clinic of CHU Sainte-Justine. Serologic testing was performed by standard methods. Upon informed consent, genotyping for Rh and Fy blood groups was also proposed to all SCD patients. DNA analyses were used to predict D phenotype (including RHD pseudogene), C, c, E, e antigen profile, FY phenotype with GATA-1 status, and to confirm critical position in the RHD (DAU cluster, zygosity) and RHCEgenes (position 48, 254, 733, 1006). The study was approved by the local Research Ethics Board (REB). Results: 203 SCD patients were evaluated: HbSS 64.3%, HbSC 29.6%, HbSb0 3.1%, HbSb+ 2.5% and HbSDIran 0.5%. ABO blood groups followed published prevalence: Group O 48.7%, A 26.2%, B 20.4%and AB 4.7%. 90.2% of patients had either a normal RHD*01 or RHD*10.00 gene which would predict a normal D+ phenotype. Twenty patients would require D− units (9.8%) to reduce their alloimmunization risk (D−, weak partial D and partial D). The RHCE genotyping results were as followed: normal c allele 61.2%, normal e allele 39.3%, partial e allele 34.1% and weak e allele 17.6%. Most RHCE alleles present in the cohort reflected variants previously reported in individuals of African descent. Most patients were compound heterozygotes. 45.8% of RHCE alleles were considered normal: RHCE*ce, RHCE*Ce and RHCE*cE. Fy(a-b-) phenotype was found in 91.6% of patients (186/203). The list of RHCE variant alleles observed in the cohort was compared to Héma-Québec's (blood supplier for the province of Quebec) African descent blood donor database. For partial e, weak partial e and rare hrB− phenotypes, only D+ donors are available whereas some of the patients are D−. The same is true for one Sec− (RH46, high-prevalence antigen) patient for which no donor is available. As for CEAG− recipients (partial ce-phenotype), two compatible blood donors were identified by screening O− units from donors of African descent. Overall, five patients (2.4%) may not have suitable donors in our blood bank based upon genotype compatibility. Twenty other patients could be added to this calculation because of a variant e allele in trans to a normal E allele. Conclusions: The RH and FY results indicate that very few patients require rare blood units. More than 90% present a normal D antigen and are Fy(a−b−), similar to most blood donors of African ancestry. However, RHCE variants could be more problematic in terms of finding compatible units. This study showed a large array of RH variants, although most are present in heterozygous form accompanied by a normal allele. This could explain the low alloimmunization rate of 3% for Rh antigens observed in this cohort (overall alloimmunization rate of 18.9%). However, as all patients were younger than 18 years old, they will probably be exposed to more blood transfusion during their adult life. Having their RH and FY genotype readily available should make the blood donors' selection easier. Further prospective studies are needed to evaluate if such an approach will lower the alloimmunization rate. Disclosures No relevant conflicts of interest to declare.

Contributions of blood groups to human genetics

1965 ◽  
Vol 163 (991) ◽  
pp. 151-168 ◽  
Keyword(s):  
Blood Transfusion ◽  
Blood Group ◽  
Group Work ◽  
Human Genetics ◽  
Blood Groups ◽  
Blood Group Antigen ◽  
Academic Environment ◽  
Experimental Side ◽  
Compatible Blood ◽  
Mendel’S Laws

Landsteiner discovered blood groups in 1900, the year in which the significance of Mendel’s work was first appreciated. Yet there was a surprisingly long interval before the ABO groups were seen to be inherited characters, characters perfectly illustrating Mendel’s laws: this took almost 10 years. Nowadays when a new blood group antigen discloses itself one of the first tasks is to work out the manner of inheritance; and to demonstrate its genetic independence of the known groups. The first three blood group systems were found in an academic environment, the fruit of experiments designed for the purpose, but all the rest are the outcome of work in blood transfusion services during the last 26 years. A few of the newer systems disclosed themselves as the result of blood-group incompatibility between foetus and mother, the foetus having inherited from its father an antigen which the mother lacks and to which she has responded by making an antibody. But most of the new systems and subdivisions of old systems are recognized as a result of the immunization of patients who have been transfused with apparently compatible blood. Blood transfusion can be thought of as the vast experimental side of blood group work.

Interrelations of Thrombo-Embolic Diseases and Blood-Group Distribution

1963 ◽  
Vol 09 (02) ◽  
pp. 472-474 ◽  
Author(s):  
W Dick ◽  
W Schneider ◽  
K Brockmüller ◽  
W Mayer
Keyword(s):  
Normal Distribution ◽  
Blood Group ◽  
Blood Groups ◽  
The Other ◽  
Other Hand ◽  
Group Distribution

SummaryA comparison between the repartition of the blood groups in 461 patients suffering from thromboembolic disorders and the normal distribution has shown a statistically ascertained predominance of the group A1. On the other hand the blood groups 0 and A2 are distinctly less frequent than in the normal distribution.

scholarly journals Associations between smoking and blood-group, and the risk of dyslipidaemia amongst French women

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
C. J. MacDonald ◽  
A. L. Madika ◽  
G. Severi ◽  
A. Fournier ◽  
M. C. Boutron-Ruault
Keyword(s):  
Blood Group ◽  
Smoking Status ◽  
Effect Modification ◽  
Blood Groups ◽  
Cross Sectional ◽  
Increased Risk ◽  
Cardio Vascular Disease ◽  
Never Smokers ◽  
Cardio Vascular ◽  
Incident Cases

AbstractDyslipidaemia is a major risk factor for cardio-vascular disease, as it promotes atherosclerosis. While cross-sectional studies have identified higher serum cholesterol amongst individuals with the A blood group, there is less evidence from prospective studies whether this translates into a higher risk of dyslipidaemia that requires treatment, nor if this genetic factor interacts with smoking status. This study aimed to prospectively determine potential associations between smoking, ABO blood groups, and risk of incident dyslipidaemia requiring treatment, and to assess associations over strata of blood ABO group. We assessed associations between blood ABO group, smoking and dyslipidaemia in 74,206 women participating in the E3N cohort. We included women who did not have cardiovascular disease at baseline. Logistic regression was used to determine associations between ABO group, smoking and prevalent dyslipidaemia at baseline. Cox proportional hazard models were then used to determine if blood ABO group and smoking were associated with the risk of incident dyslipidaemia, amongst women free of dyslipidaemia at baseline. At baseline 28,281 women with prevalent dyslipidaemia were identified. Compared to the O-blood group, the non-O blood group was associated higher odds of with prevalent dyslipidaemia (ORnon-O = 1.09 [1.06: 1.13]). Amongst the women free of dyslipidaemia at baseline, 6041 incident cases of treated dyslipidaemia were identified during 454,951 person-years of follow-up. The non-O blood groups were associated with an increased risk of dyslipidaemia when compared to the O-group (HRnon-O = 1.16 [1.11: 1.22]), specifically the A blood-group (HRA = 1.18 [1.12: 1.25]). Current smokers were associated with an increased risk of incident dyslipidaemia (HR smokers = 1.27 [1.16: 1.37]), compared to never-smokers. No evidence for effect modification between smoking and ABO blood group was observed (p-effect modification = 0.45), although the highest risk was observed among AB blood group women who smoked (HR = 1.76 [1.22: 2.55]). In conclusion, the non-O blood groups, specifically the A group were associated with an increased risk of dyslipidaemia. Current smokers were associated with a 30% increased risk of dyslipidaemia. These results could aid in personalised approaches to the prevention of cardiovascular risk-factors.

An Insight into the General Trend of Blood Group Dissemination among Local Population of Muzaffarabad

2020 ◽  
pp. 39-43
Author(s):  
OJS Admin
Keyword(s):  
Blood Group ◽  
Disease Susceptibility ◽  
General Trend ◽  
Local Population ◽  
Blood Groups ◽  
Blood Group System ◽  
Group System ◽  
Insight Into

Blood groups ABO and Rhesus, constituting the most principal blood group system, are of key signicance for clinical and transfusion practices and are moreover, thought to be associated with disease susceptibility.

scholarly journals Correlation of ABO Blood Groups and Rh Factor with The Severity of Generalized Chronic Periodontitis: Across Sectional Study in Riyadh, Saudi Arabia

2019 ◽  
Vol 7 (4) ◽  
pp. 617-622 ◽  
Author(s):  
Diana Mostafa ◽  
Essam I. Elkhatat ◽  
Pradeep Koppolu ◽  
Muna Mahgoub ◽  
Esam Dhaifullah ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Blood Group ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Blood Groups ◽  
Blood Group Antigens ◽  
Abo Blood Groups ◽  
Sectional Study ◽  
Cross Sectional ◽  
Group I

BACKGROUND: The development of periodontal diseases depends on the presence of causative microorganisms, host immunity and risk factors. Although variability present among the types of periodontal diseases, all are represented to a shared interaction between host and bacteria. ABO blood groups are the most investigated erythrocyte antigen system. However, limited investigations have been conducted to explore the alliance between ABO blood groups and periodontal diseases. AIM: Our purpose was to explore any possible association between the severity of chronic periodontitis with ABO blood groups and Rh factor. METHODS: A cross-sectional study was carried out on 205 patients out of 1126 generalised chronic periodontitis patients (GCP) who were referred to Al-Farabi Colleges, Riyadh, Saudi Arabia. They were categorized into; group I (mild), group II (moderate) and group III (sever). RESULTS: The patients with blood group O were at a greater risk to develop GCP irrespective of its severity, followed by those with blood group A, B, and AB. The dispensation of the Rh factor in all groups exhibited a significantly greater distribution of Rh positive. CONCLUSION: Genetic factors such as ABO blood group antigens may act as a risk influencer that affects the progression and severity of the chronic periodontitis.

scholarly journals Distribution of ABO and Rh blood groups in Nepalese medical students: a report

2000 ◽  
Vol 6 (1) ◽  
pp. 156-158
Author(s):  
T. Pramanik ◽  
S. Pramanik
Keyword(s):  
Medical Students ◽  
Blood Group ◽  
Blood Groups ◽  
Blood Group A ◽  
Group A ◽  
Group B ◽  
Group Distribution

The frequencies of ABO and rhesus blood groups vary from one population to another. We studied blood group distribution in 120 Nepalese students; 34% were blood group A, 29% group B, 4% group AB and 32.5% group O. The frequency of Rh-negative blood was 3.33% and Rh-positive 96.66%

scholarly journals Investigation of association between ABO blood groups and COVID-19 clinical severity

2021 ◽  
Vol 8 (12) ◽  
pp. 673-676
Author(s):  
Arzu İrvem ◽  
Abdurrahman Sarmış ◽  
Özlem Akgün Doğan ◽  
Jale Yıldız ◽  
Zafer Habib ◽  
...  
Keyword(s):  
Risk Factors ◽  
Blood Group ◽  
Blood Groups ◽  
Ct Imaging ◽  
Clinical Severity ◽  
Imaging Findings ◽  
Mortality And Morbidity ◽  
Significant Difference ◽  
Important Center ◽  
Severity Of The Disease

Objective: COVID-19 has been detected in Turkey since March 11, 2020. Istanbul has become an important center of the pandemic in Turkey. Various risk factors for COVID-19 infection, mortality, and morbidity are under investigation. Recent studies have suggested that certain blood groups are risk factors for the disease. The aim of this study is the evaluation the relationship between blood groups and the risk of contracting COVID-19 disease, clinical severity of the disease, and CT (computed tomography) imaging findings. Material and Methods: Age, gender, blood group data, clinical severity and CT images of 300 patients who were positive with RT PCR (Reverse transcription-polymerase chain reaction) and were followed up in the clinic were retrospectively scanned and recorded. The clinical severity of the disease and CT imaging findings were scored, and the data were evaluated statistically. Results: While the incidence of COVID-19 was high in the A blood group, it was low in the 0 blood group. Although there was no significant difference between blood types and clinical severity, the involvement in the B blood group was more severe on CT imaging. Conclusion: People with A blood group should pay more attention to protection and isolation. Investigating this difference and underlying pathogenic mechanisms can guide science with advanced studies.

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