SOD polymorphism in the Xiphinema americanum-group (Nematoda: Longidoridae)

AbstractIsoelectrofocusing of superoxide dismutase (SOD) isoforms was carried out on the extracts of 117 nematode populations belonging to the so-called Xiphinema americanum-group. These populations came from the USA (77), Chile (5), Argentina (1), Venezuela (5), Portugal (15), Italy (2), Crete (1), Montenegro (1), Slovakia (4), Hungary (3), Egypt (1) and India (2). A total of 17 bands of enzyme activity were observed in the screening, whilst single enzyme phenotypes showed from two to eight bands. The high degree of SOD polymorphism of this nematode collection was grouped by cluster analysis into seven distinct homogeneous groups characterised by specific combinations of SOD markers. Sub-groups could be discriminated for larger groups. The small Groups 3 and 5 were constituted mostly by populations from USA east coast states (i.e., NY and PA, respectively). The larger Group 1 resulted from the association of populations coming from various and distant North American States. In other large groups, North American populations were associated with South American and European populations. Overall, the data presented here suggest that geographic separation and different hosts do not seem to be the source of genetic diversity for the X. americanum-group. When an adequate number of populations were collected from the same country, the variability expressed by such a sub-sample was comparable to that of the whole nematode collection. For the first time, homogeneous populations of a large collection of X. americanum-group populations were associated by molecular means in order to explore further approaches that may help resolve the recalcitrant taxonomy and phylogeny of this much debated group.

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New and unusual host records for North American and South American spider wasps (Hymenoptera: Pompilidae)

Zootaxa ◽

10.11646/zootaxa.4891.1.1 ◽

2020 ◽

Vol 4891 (1) ◽

pp. 1-112

Author(s):

FRANK E. KURCZEWSKI ◽

RICK C. WEST ◽

CECILIA WAICHERT ◽

KELLY C. KISSANE ◽

DARRELL UBICK ◽

...

Keyword(s):

United States ◽

South America ◽

Central America ◽

North American ◽

Western Hemisphere ◽

South American ◽

New Host ◽

United States Mexico ◽

First Time ◽

Taxonomic Order

New and unusual host records for 133 species and subspecies of Pompilidae predominantly from the southwestern United States, Mexico, Central America, and South America are presented in modified taxonomic order. First-time species host records are given for Calopompilus Ashmead, Pepsis Fabricius, Hemipepsis Dahlbom, Priocnessus Banks, Entypus Dahlbom, Pompilocalus Roig-Alsina, Sphictostethus Kohl, Auplopus Spinola, Ageniella Banks, Eragenia Banks, Aporus Spinola, Poecilopompilus Ashmead, Tachypompilus Ashmead, Anoplius Dufour, Priochilus (Fabricius) and Notocyphus Smith. New host spider families are introduced for Calopompilus, Pepsis, Hemipepsis, Priocnessus, Entypus, Cryptocheilus Panzer, Priocnemis Schiødte, Auplopus, Ageniella, Eragenia, Aporus, Tachypompilus, Anoplius, Priochilus and Notocyphus. Eight host spider families are reported from the Western Hemisphere for the first time: Halonoproctidae (Notocyphus dorsalis dorsalis Cresson); Dipluridae (Pepsis pretiosa Dahlbom, P. montezuma Smith, P. infuscate Spinola, P. atripennis Fabricius, P. martini Vardy, Priocnessus vancei Waichert and Pitts); Nemesiidae (Pepsis pallidolimbata Lucas, P. viridis Lepeletier, P. spp., Pompilocalus hirticeps (Guérin), Sphictostethus gravesii (Haliday), S. striatulus Roig-Alsina, Priocnemis oregona Banks); Barychelidae (Eragenia sp.); Paratropididae (Pepsis stella Montet); Trechaleidae (Hemipepsis toussainti (Banks), Entypus unifasciatus cressoni (Banks), Tachypompilus ferrugineus (Say), Tachypompilus unicolor cerinus Evans, Priochilus gloriosum (Cresson); Desidae (Ageniella accepta (Cresson), Sphictostethus isodontus Roig-Alsina) and Selenopidae (Priochilus scrupulum (Fox), Tachypompilus erubescens (Taschenberg) or xanthopterus (Rohwer)). The first known host records for the rare South American pompilid genera Chirodamus (Lycosidae: Lycosa sp.) and Herbstellus (Nemesiidae: Diplothelopsis cf bonariensis Mello-Leitão) are presented.

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Trametes gibbosa (Basidiomycetes, Polyporales) in the USA and Canada

Canadian Journal of Botany ◽

10.1139/b07-013 ◽

2007 ◽

Vol 85 (3) ◽

pp. 342-346 ◽

Cited By ~ 5

Author(s):

J. Kout ◽

J. Vlasák

Keyword(s):

United States ◽

Czech Republic ◽

North America ◽

Mating Type ◽

North American ◽

Eastern North America ◽

The Czech Republic ◽

The North ◽

The Usa ◽

First Time

The polypore Trametes gibbosa (Pers.) Fries, common in Europe and Asia, is reported from eastern North America for the first time. Single basidiospore cultures from Pennsylvania, United States, and Quebec, Canada, were paired with each other and with cultures from the Czech Republic. The North American intercollection crosses were 60% compatible and 100% compatible with the Czech cultures. All the crosses among the Czech cultures were 100% compatible. The recent introduction of T. gibbosa to North America is suggested as a possible explanation for the limited number of mating-type alleles and subsequent incompatibility among the North American cultures.

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Description of a fossil camelid from the Pleistocene of Argentina, and a cladistic analysis of the Camelinae

Swiss Journal of Palaeontology ◽

10.1186/s13358-020-00208-6 ◽

2020 ◽

Vol 139 (1) ◽

Author(s):

Sinéad Lynch ◽

Marcelo R. Sánchez-Villagra ◽

Ana Balcarcel

Keyword(s):

New Species ◽

North American ◽

Cladistic Analysis ◽

Species Level ◽

Phylogenetic Position ◽

South American ◽

Lama Guanicoe ◽

Vicugna Vicugna ◽

First Time ◽

A New Species

AbstractWe describe a well-preserved South American Lamini partial skeleton (PIMUZ A/V 4165) from the Ensenadan (~ 1.95–1.77 to 0.4 Mya) of Argentina. The specimen is comprised of a nearly complete skull and mandible with full tooth rows, multiple elements of anterior and posterior limbs, and a scapula. We tested this specimen’s phylogenetic position and hypothesized it to be more closely related to Lama guanicoe and Vicugna vicugna than to Hemiauchenia paradoxa. We formulate a hypothesis for the placement of PIMUZ A/V 4165 within Camelinae in a cladistic analysis based on craniomandibular and dental characters and propose that future systematic studies consider this specimen as representing a new species. For the first time in a morphological phylogeny, we code terminal taxa at the species level for the following genera: Camelops, Aepycamelus, Pleiolama, Procamelus, and Alforjas. Our results indicate a divergence between Lamini and Camelini predating the Barstovian (16 Mya). Camelops appears as monophyletic within the Camelini. Alforjas taylori falls out as a basal member of Camelinae—neither as a Lamini nor Camelini. Pleiolama is polyphyletic, with Pleiolama vera as a basal Lamini and Pleiolama mckennai in a more nested position within the Lamini. Aepycamelus and Procamelus are respectively polyphyletic and paraphyletic. Together, they are part of a group of North American Lamini from the Miocene epoch.

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9/11 and Armageddon: the Christian Right and George W. Bush

Holy Land Studies ◽

10.3366/hls.2003.0009 ◽

2003 ◽

Vol 2 (1) ◽

pp. 33-50

Author(s):

Göran Gunner

Keyword(s):

New York ◽

Christian Right ◽

Political Life ◽

Religious Leader ◽

The European Union ◽

The World ◽

Mega Church ◽

The Usa ◽

Jerry Falwell ◽

High Degree

Authors from the Christian Right in the USA situate the September 11 attack on New York and Washington within God's intentions to bring America into the divine schedule for the end of the world. This is true of Pat Robertson and Jerry Falwell, and other leading figures in the ‘Christian Coalition’. This article analyses how Christian fundamentalists assess the roles of the USA, the State of Israel, Islam, Iraq, the European Union and Russia within what they perceive to be the divine plan for the future of the world, especially against the background of ‘9/11’. It argues that the ideas of the Christian Right and of President George W. Bush coalesce to a high degree. Whereas before 9/11 many American mega-church preachers had aspirations to direct political life, after the events of that day the President assumes some of the roles of a mega-religious leader.

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More Christian Apocrypha

Bulletin for the Study of Religion ◽

10.1558/bsor.v41i3.16 ◽

2012 ◽

Vol 41 (3) ◽

pp. 16-21

Author(s):

Tony Burke

Keyword(s):

New Testament ◽

Old Testament ◽

North American ◽

English Language ◽

Primary Sources ◽

University Of Edinburgh ◽

The University ◽

First Time ◽

Medieval Christianity

Scholars interested in the Christian Apocrypha (CA) typically appeal to CA collections when in need of primary sources. But many of these collections limit themselves to material believed to have been written within the first to fourth centuries CE. As a result a large amount of non-canonical Christian texts important for the study of ancient and medieval Christianity have been neglected. The More Christian Apocrypha Project will address this neglect by providing a collection of new editions (some for the first time) of these texts for English readers. The project is inspired by the More Old Testament Pseudepigrapha Project headed by Richard Bauckham and Jim Davila from the University of Edinburgh. Like the MOTP, the MCAP is envisioned as a supplement to an earlier collection of texts—in this case J. K. Elliott’s The Apocryphal New Testament (Oxford 1991), the most recent English-language CA collection (but now almost two decades old). The texts to be included are either absent in Elliott or require significant revision. Many of the texts have scarcely been examined in over a century and are in dire need of new examination. One of the goals of the project is to spotlight the abilities and achievements of English (i.e., British and North American) scholars of the CA, so that English readers have access to material that has achieved some exposure in French, German, and Italian collections.

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Diversification of the North American Doellingeria-Eucephalus Clade (Astereae: Asteraceae) Inferred from Molecular and Morphological Evidence

Systematic Botany ◽

10.1600/036364419x15710776741477 ◽

2019 ◽

Vol 44 (4) ◽

pp. 930-942

Author(s):

Geraldine A. Allen ◽

Luc Brouillet ◽

John C. Semple ◽

Heidi J. Guest ◽

Robert Underhill

Keyword(s):

North American ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Sister Group ◽

Morphological Evidence ◽

South American ◽

New Combinations ◽

The North ◽

Hybridization Event ◽

Ancient Hybridization

Abstract—Doellingeria and Eucephalus form the earliest-diverging clade of the North American Astereae lineage. Phylogenetic analyses of both nuclear and plastid sequence data show that the Doellingeria-Eucephalus clade consists of two main subclades that differ from current circumscriptions of the two genera. Doellingeria is the sister group to E. elegans, and the Doellingeria + E. elegans subclade in turn is sister to the subclade containing all remaining species of Eucephalus. In the plastid phylogeny, the two subclades are deeply divergent, a pattern that is consistent with an ancient hybridization event involving ancestral species of the Doellingeria-Eucephalus clade and an ancestral taxon of a related North American or South American group. Divergence of the two Doellingeria-Eucephalus subclades may have occurred in association with northward migration from South American ancestors. We combine these two genera under the older of the two names, Doellingeria, and propose 12 new combinations (10 species and two varieties) for all species of Eucephalus.

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TAMM HORSFALL PROTEIN: THE MEN BEHIND THE EPONYM

Nephrology (Saint-Petersburg) ◽

10.24884/1561-6274-2019-23-2-117-119 ◽

2019 ◽

Vol 23 (2) ◽

pp. 117-119 ◽

Cited By ~ 1

Author(s):

D. N. Paskalev ◽

B. T. Galunska ◽

D. Petkova-Valkova

Keyword(s):

Viral Replication ◽

Research Team ◽

Molecular Mechanisms ◽

Thick Ascending Limb ◽

Rockefeller Institute ◽

The Usa ◽

Natural Inhibitor ◽

Simplex Virus ◽

First Time ◽

New Protein

Tamm–Horsfall Protein (uromodulin) is named after Igor Tamm and Franc Horsfall Jr who described it for the first time in 1952. It is a glycoprotein, secreted by the cells in the thick ascending limb of the loop of Henle. This protein will perform a number of important pathophysiological functions, including protection against uroinfections, especially caused by E. Сoli, and protection against formation of calcium concernments in the kidney. Igor Tamm (1922-1995) is an outstanding cytologist, virologist and biochemist. He is one of the pioneers in the study of viral replication. He was born in Estonia and died in the USA. In 1964 he was elected for a professorship in Rockefeller Institute for Medical Research, where has been working continuously. Since 1959, he became a head of the virology lab established by his mentor and co-author Franc Horsfall. In the course of studies on the natural inhibitor of viral replication, Tamm and Horsfall isolated and characterized biochemically a new protein named after their names. Franc Lappin Horsfall Jr (1906-1971) was a well-known clinician and virologist with remarkable achievements in internal medicine. He was born and died in the USA. He worked in the Rockefeller Hospital from 1934 to 1960, then in the Center for Cancer Research at the Sloan-Kettering Institute. Here he was a leader of a research team studying the molecular mechanisms of immunity, the effects of chemotherapy with benzimidazole compounds (together with I. Tamm), coxsackie viruses, herpes simplex virus, etc.

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Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽

10.21273/hortsci.33.3.492f ◽

1998 ◽

Vol 33 (3) ◽

pp. 492f-493

Author(s):

Roberto F. Vieira ◽

James E. Simon ◽

Peter Goldsbrough ◽

Antonio Figueira

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Rapd Markers ◽

Volatile Oil ◽

Essential Oil Composition ◽

Oil Composition ◽

Traditional Medicines ◽

The Tropics ◽

High Degree ◽

First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

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Nucleotide polymorphisms in three genes support host and geographic speciation in tree pathogens belonging toGremmeniellaspp.

Canadian Journal of Botany ◽

10.1139/b02-103 ◽

2002 ◽

Vol 80 (11) ◽

pp. 1151-1159 ◽

Cited By ~ 8

Author(s):

M Dusabenyagasani ◽

G Laflamme ◽

R C Hamelin

Keyword(s):

North America ◽

Dna Sequences ◽

North American ◽

Abies Balsamea ◽

Host Specialization ◽

Rrna Genes ◽

Nucleotide Polymorphisms ◽

Group I ◽

Geographic Separation ◽

Pinus Spp

We detected nucleotide polymorphisms within the genus Gremmeniella in DNA sequences of β-tubulin, glyceraldehyde phosphate dehydrogenase, and mitochondrial small subunit rRNA (mtSSU rRNA) genes. A group-I intron was present in strains originating from fir (Abies spp.) in the mtSSU rRNA locus. This intron in the mtSSU rRNA locus of strains isolated from Abies sachalinensis (Fridr. Schmidt) M.T. Mast in Asia was also found in strains isolated from Abies balsamea (L.) Mill. in North America. Phylogenetic analyses yielded trees that grouped strains by host of origin with strong branch support. Asian strains of Gremmeniella abietina (Lagerberg) Morelet var. abietina isolated from fir (A. sachalinensis) were more closely related to G. abietina var. balsamea from North America, which is found on spruce (Picea spp.) and balsam fir, and European and North American races of G. abietina var. abietina from pines (Pinus spp.) were distantly related. Likewise, North American isolates of Gremmeniella laricina (Ettinger) O. Petrini, L.E. Petrini, G. Laflamme, & G.B. Ouellette, a pathogen of larch, was more closely related to G. laricina from Europe than to G. abietina var. abietina from North America. These data suggest that host specialization might have been the leading evolutionary force shaping Gremmeniella spp., with geographic separation acting as a secondary factor.Key words: Gremmeniella, geographic separation, host specialization, mitochondrial rRNA, nuclear genes.

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Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea

Pathogens ◽

10.3390/pathogens10040425 ◽

2021 ◽

Vol 10 (4) ◽

pp. 425

Author(s):

Hyung-Woo Kang ◽

Eun-Yong Lee ◽

Kyoung-Ki Lee ◽

Mi-Kyeong Ko ◽

Ji-Young Park ◽

...

Keyword(s):

Nucleotide Sequence ◽

South Korea ◽

Molecular Level ◽

Economic Losses ◽

Equine Herpesvirus ◽

Equine Herpesvirus 1 ◽

Mlst Analysis ◽

Herpesvirus 1 ◽

First Time ◽

Group 1

Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7–100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701–818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.

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