scholarly journals <i>In Vitro</i> Antimicrobial Characterization of <i>Lactobacillus</i> Isolates Towards Their Use as Probiotic Alternatives to Antibiotic Growth Promoters

2019 ◽  
Vol 4 (3) ◽  
pp. 72
Author(s):  
Raoul Emeric Guetiya Wadoum ◽  
Fonteh Anyangwe Florence ◽  
Kaktcham Pierre Marie ◽  
Ulrich Landry Bemmo Kamdem ◽  
Chancel Hector Momo Kenfack ◽  
...  
Keyword(s):  
Growth Promoters ◽  
Antibiotic Growth Promoters

scholarly journals The Biological Activity of Monarda didyma L. Essential Oil and Its Effect as a Diet Supplement in Mice and Broiler Chicken

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3368
Author(s):  
Héloïse Côté ◽  
André Pichette ◽  
Alexis St-Gelais ◽  
Jean Legault
Keyword(s):  
Body Weight ◽  
Essential Oil ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Growth Promoters ◽  
Antibiotic Growth Promoters ◽  
Monarda Didyma

The use of growth-promoting antibiotics in livestock faces increasing scrutiny and opposition due to concerns about the increased occurrence of antibiotic-resistant bacteria. Alternative solutions are being sought, and plants of Lamiaceae may provide an alternative to synthetic antibiotics in animal nutrition. In this study, we extracted essential oil from Monarda didyma, a member of the Lamiaceae family. We examined the chemical composition of the essential oil and then evaluated the antibacterial, antioxidant, and anti-inflammatory activities of M. didyma essential oil and its main compounds in vitro. We then evaluated the effectiveness of M. didyma essential oil in regard to growth performance, feed efficiency, and mortality in both mice and broilers. Carvacrol (49.03%) was the dominant compound in the essential oil extracts. M. didyma essential oil demonstrated antibacterial properties against Escherichia coli (MIC = 87 µg·mL−1), Staphylococcus aureus (MIC = 47 µg·mL−1), and Clostridium perfringens (MIC = 35 µg·mL−1). Supplementing the diet of mice with essential oil at a concentration of 0.1% significantly increased body weight (+5.4%) and feed efficiency (+18.85%). In broilers, M. didyma essential oil significantly improved body weight gain (2.64%). Our results suggest that adding M. didyma essential oil to the diet of broilers offers a potential substitute for antibiotic growth promoters.

scholarly journals In vitro Antibacterial Efficacy of Non-Antibiotic Growth Promoters in Poultry Industry

2020 ◽  
Vol 57 (1) ◽  
pp. 45-54
Author(s):  
Mashael R. Aljumaah ◽  
Manal M. Alkhulaifi ◽  
Alaeldein M. Abudabos
Keyword(s):  
Antibacterial Efficacy ◽  
Poultry Industry ◽  
Growth Promoters ◽  
Antibiotic Growth Promoters

scholarly journals Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
UMI FATMAWATI ◽  
ANJA MERYANDINI ◽  
ABDJAD ASIH NAWANGSIH ◽  
Aris Tri Wahyudi
Keyword(s):  
Plant Growth ◽  
Lateral Roots ◽  
Dry Weight ◽  
Vital Role ◽  
Growth Promoters ◽  
16S Rrna Sequence ◽  
Streptomyces Spp ◽  
Phosphate Solubilizing

Abstract. Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. Actinomycetes which colonized plant rhizosphere has a vital role in improving plant growth by producing Indole-3-Acetic Acid (IAA). The aim of this study was to evaluate the potency of actinomycetes isolated from soybean rhizosphere as promoter agents for plant growth in vitro. Fifty actinomycetes isolates were successfully isolated from soybean rhizosphere. Based on the colorimetric methods, 35 isolates can produce IAA in various concentration, in the range of 0.46-30.6 mg/L. Seed germination assay using Ragdoll methods revealed that 26 isolates significantly promoted germination parameters, including the hypocotyl and the radicular length, the number of the lateral roots, and dry weight of the plant. Also, 14 from 26 isolates showed phosphate solubilizing activity in different phosphate-solubilizing index ranging from 1.25-2.62. Eight isolates were able to grow in N-free medium, indicating that these isolates have the ability in fixing nitrogen. Out of 23 from 26 isolates were detected to produce siderophore. All the tested isolates show chitinase production except ASR 55. Based on the observed parameters, it showed that there are four potential isolates (ASR 46, ASR 58, ASR 75 and ASR 76) as promising plant-growth promoters, phosphate solubilizer, nitrogen fixer, siderophore and chitinase producer. Based on the result of 16S rRNA sequence analysis, four potential isolates were identified as Streptomyces spp. in different taxa of strains and species.

scholarly journals Study In Vitro: Kombinasi Herbal dan Probiotik sebagai Alternatif Antibiotic Growth Promoter

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Vinsa Cantya Prakasita
Keyword(s):  
Animal Feed ◽  
Bifidobacterium Longum ◽  
Growth Zone ◽  
Diffusion Method ◽  
Growth Promoter ◽  
Growth Promoters ◽  
Antibiotic Growth Promoters ◽  
The Government

Antibiotic growth promoters (AGP) are widely used in animal feed for feed efficiency, stimulate growth, productivity and minimize mortality by preventing infection, but now its use has been officially banned by the government. As a result of the ban, many farmers or animal feed producer seek solutions in various ways such as the addition of herbs, probiotics, and a combination of both, but there are still few reports on the role of herbs, probiotics and a combination of both for alternative substitutes for AGP. This study aims to determine the role of herbs in the growth of probiotics. Herbs used ginger, turmeric, galingale, wild ginger, and temuireng, while the probiotics used are Bifidobacterium longum, B. bifidum, Bacillus sp., and Saccharomyces cereviciae. The benefits of herbs in supporting probiotics were tested using the disc diffusion method. Chloramphenicol disc ware used as control positive. Observation and measurement of the growth zone of probiotics were carried out after incubation. The results of this study prove that herbs do not inhibit the growth of probiotics. Herbal content can be a prebiotic candidate that can support the growth of probiotics. The combination of herbal-probiotics can potentially be an AGP substitute in animal feed.

Assays for Phospholipid-Dependent Formation of Thrombin and Xa: A Potential Method for Quantifying Lupus Anticoagulant Activity

1991 ◽  
Vol 66 (04) ◽  
pp. 453-458 ◽  
Author(s):  
John T Brandt
Keyword(s):  
Specific Activity ◽  
Anticoagulant Activity ◽  
Factor Xa ◽  
Clinical Importance ◽  
Potential Method ◽  
Quantitative Expression ◽  
Increased Risk ◽  
Apparent Association

SummaryLupus anticoagulants (LAs) are antibodies which interfere with phospholipid-dependent procoagulant reactions. Their clinical importance is due to their apparent association with an increased risk of thrombo-embolic disease. To date there have been few assays for quantifying the specific activity of these antibodies in vitro and this has hampered attempts to purify and characterize these antibodies. Methods for determining phospholipid-dependent generation of thrombin and factor Xa are described. Isolated IgG fractions from 7 of 9 patients with LAs were found to reproducibly inhibit enzyme generation in these assay systems, permitting quantitative expression of inhibitor activity. Different patterns of inhibitory activity, based on the relative inhibition of thrombin and factor Xa generation, were found, further substantiating the known heterogeneity of these antibodies. These systems may prove helpful in further purification and characterization of LAs.

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