Management of Osteoarthritis Using 1 Intra-articular Platelet Concentrate Administration in a Canine Osteoarthritis Model

Background: Osteoarthritis (OA) represents a significant burden to societies, as it affects quality of life and performance and implies a large cost in terms of health care. Autologous platelets are a regenerative treatment modality for OA that are thought to be a potential stimulation of the natural healing cascade. Purpose: To describe the effect of the platelet concentrate V-PET in the management of OA in a naturally occurring canine model, using several outcome assessment modalities. Study Design: Controlled laboratory study. Methods: A total of 40 joints of active working police dogs with hip OA were randomly assigned to a control group (CG) and a platelet concentrate group (PCG; treatment) and evaluated. At treatment day (T0) and 8, 15, 30, 90, and 180 days after treatment, weight distribution, joint range of motion at flexion and extension, thigh girth, digital thermography, radiographic signs, 4 clinical metrology instruments, and synovial fluid interleukin 1 and C-reactive protein levels were recorded. Results were compared using repeated-measures analysis of variance with a Huynh-Feldt correction, paired-samples t test, or Wilcoxon signed rank test, with P < .05. Results: Dogs were 6.5 ± 2.4 years old (mean ± SD) and had a body weight of 26.7 ± 5.2 kg. At T0, 32 (80%) joints were graded as having mild OA, 6 (15%) as moderate, and 2 (5%) as severe. No differences were found between groups at T0. Between the PCG and CG, the symmetry index showed significant improvements in the PCG from 8 days ( P = .01) to 180 days ( P = .01). Joint flexion also improved in the PCG up to 90 days ( P < .05) and extension improved up to 180 days ( P < .01). Several clinical metrology instrument scores also improved up to 90 to 180 days after treatment. In the CG, radiographic signs progressed, while the PCG showed some improved signs. In both groups, increasing body weight and age corresponded with worse clinical and laboratory findings. Conclusion: A single injection of platelet concentrate had a positive effect, lasting up to 6 months, on several clinical, imaging, and laboratory signs in a naturally occurring canine OA model. Clinical Relevance: We characterized the effects of this platelet concentrate in dogs, considered the gold standard of the study of OA, with a group of working animals with similar high demands as athletes.

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The intra-articular administration of triamcinolone hexacetonide in the treatment of osteoarthritis. Its effects in a naturally occurring canine osteoarthritis model

PLoS ONE ◽

10.1371/journal.pone.0245553 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0245553

Author(s):

João C. Alves ◽

Ana Santos ◽

Patrícia Jorge ◽

Catarina Lavrador ◽

L. Miguel Carreira

Keyword(s):

Weight Distribution ◽

Repeated Measures ◽

Weight Bearing ◽

Interleukin 1 ◽

Response To Treatment ◽

Control Group ◽

Radiographic Findings ◽

Triamcinolone Hexacetonide ◽

Paired Samples ◽

Kaplan Meier

Objective To evaluate the effect of an intra-articular (IA) administration of triamcinolone hexacetonide, compared with saline. Patients and methods Forty (N = 40) hip joints were randomly assigned to a treatment group (THG, n = 20, receiving IA triamcinolone hexacetonide) and a control group (CG, n = 20, receiving IA saline). On treatment day (T0), and at 8, 15, 30, 90 and 180 days post-treatment, weight distribution, joint range of motion, thigh girth, digital thermography, radiographic signs, synovial fluid interleukin-1 and C-reactive protein levels were evaluated. Data from four Clinical Metrology Instruments was also gathered. Results were compared Repeated Measures ANOVA, with a Huynh-Feldt correction, Paired Samples T-Test or Wilcoxon Signed Ranks Test. A Kaplan-Meier test was performed to compare both groups, with p<0.05. Results Joints were graded as mild (65%), moderate (20%) and severe (15%). Patients of both sexes, with a mean age of 6.5±2.4 years and bodyweight of 26.7±5.2kg, were included. No differences were found between groups at T0. Comparing THG to CG, weight distribution showed significant improvements in THG from 8 (p = 0.05) up to 90 days (p = 0.01). THG showed lower values during thermographic evaluation in the Lt view (p<0.01). Pain and function scores also improved from 30 to 180 days. Increasing body weight, age, and presence of caudolateral curvilinear osteophyte corresponded to worse response to treatment. Results of the Kaplan Meier test showed significant differences between groups, with THG performing better considering several evaluations and scores. Conclusion THG recorded significant improvements in weight-bearing and in with the considered CMIs, particularly pain scores. Lower thermographic values were registered in THG up to the last evaluation day. Age, sex, and radiographic findings did significantly influenced response to treatment.

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Effect of a single intra-articular high molecular weight hyaluronan in a naturally occurring canine osteoarthritis model: a randomized controlled trial

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-021-02423-4 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

J. C. Alves ◽

Ana Margarida Moniz Pereira dos Santos ◽

Patrícia Jorge ◽

Catarina Falcão Trigoso Vieira Bran Lavrador ◽

L. Miguel Carreira

Keyword(s):

Repeated Measures ◽

Financial Burden ◽

Controlled Trial ◽

Interleukin 1 ◽

Well Being ◽

Joint Disease ◽

Response To Treatment ◽

Control Group ◽

Paired Samples ◽

Treatment Experiment

Abstract Background Osteoarthritis (OA) is a complex joint disease and chronic pain source, affecting a patient’s quality of life and posing a financial burden. As the dog is considered a nearly ideal species for translation research of human OA and the most used model for research, exploring spontaneous dog OA under the One Health/One Medicine concept can improve both humans and dogs’ health and well-being. Methods In a clinical treatment experiment, forty (N=40) joints were selected and randomly assigned to a control group (CG), which received 0.9% NaCl or a treatment (HG), which received Hylan G-F 20. Evaluations were performed on treatment day (T0), 8, 15, 30, 90, and 180 days post-treatment. They consisted of four different Clinical Metrology Instruments (CMI), evaluation of weight distribution, joint range of motion, thigh girth, radiographic and digital thermography imaging, synovial fluid interleukin-1 (IL-1), and C-reactive protein concentrations. Results were compared with repeated measures ANOVA, with a Huynh-Feldt correction, Paired samples T-test, or Wilcoxon signed-ranks test, with p<0.05. Results Patients had a mean age of 6.5±2.4 years and a bodyweight of 26.6±5.2kg, and joints graded as mild (n=28, 70%), moderate (n=6, 15%), and severe OA (n=6, 15%). No differences were found between groups at T0. Symmetry index and deviation showed significant improvements in HG from 30 days (p<0.01) up to 180 days (p=0.01). Several CMI scores, particularly pain scores, improved from 90 to 180 days. Radiographic signs progressed in both groups. In both groups, increasing body weight and age corresponded to worse clinical presentation. IA hyaluronan administration produced increased lameness in six cases, which resolved spontaneously. Conclusions This study characterizes the response to treatment with Hylan G-F 20, which can produce significant functional and pain level improvements in patients with OA, even those with factors related to worse response to treatment.

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Impact of Mindfulness Training on Spanish Police Officers’ Mental and Emotional Health: a Non-Randomized Pilot Study

Mindfulness ◽

10.1007/s12671-022-01827-5 ◽

2022 ◽

Author(s):

Jaime Navarrete ◽

Miguel Ángel García-Salvador ◽

Ausiàs Cebolla ◽

Rosa Baños

Keyword(s):

Police Officers ◽

Repeated Measures ◽

Emotional Health ◽

Analysis Of Covariance ◽

Controlled Study ◽

Group Differences ◽

Control Group ◽

Depression Symptoms ◽

Control Group Design ◽

Paired Samples

Abstract Objectives The purpose of this exploratory non-randomized controlled study was to determine the acceptance and effectiveness of an 8-week mindfulness-based intervention (MBI) co-designed by a police officer. Methods A pretest-posttest control group design was followed. Participants (MBI group = 20; control group = 18) answered baseline and post-training self-reported measures. In addition, the weekly emotional state of the MBI group was collected. Paired-samples t-test and analysis of covariance were performed for pre-post within-group and between-group differences, respectively, as well as linear mixed effects analysis of repeated measures for week-by-week data. Results High acceptance and attendance rates, as well as significant pre-post within-group differences in the MBI group in mindfulness (η2 = 0.43), self-compassion (η2 = 0.43), depression (η2 = 0.54), anxiety (η2 = 0.46), stress (η2 = 0.51), difficulties in emotion regulation, sleep quality (η2 = 0.57), and burnout (η2 = 0.31–0.47), were identified. Moreover, police officers who underwent the MBI experienced a week by week decrease of anger, disgust, anxiety, sadness, and desire. Finally, after adjusting for pre-test scores, significant between-group differences were found in the way of attending to internal and external experiences (observing mindfulness facet; ηp2 = 0.21), depression symptoms (ηp2 = 0.23), general distress (ηp2 = 0.24), and the degree of physical and psychological exhaustion (personal burnout; ηp2 = 0.20). Conclusions The preliminary effectiveness of this MBI on psychopathology and quality of life outcomes in Spanish police officers was discussed. Previous evidence regarding the promising use of MBIs in this population was supported.

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Inverse Occlusion: A Binocularly Motivated Treatment for Amblyopia

Neural Plasticity ◽

10.1155/2019/5157628 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 9

Author(s):

Jiawei Zhou ◽

Zhifen He ◽

Yidong Wu ◽

Yiya Chen ◽

Xiaoxin Chen ◽

...

Keyword(s):

Ocular Dominance ◽

Contrast Ratio ◽

Primary Outcome Measure ◽

Secondary Outcome ◽

Rank Test ◽

Short Term ◽

Laboratory Findings ◽

Paired Samples ◽

Signed Rank Test ◽

Refractive Correction

Recent laboratory findings suggest that short-term patching of the amblyopic eye (i.e., inverse occlusion) results in a larger and more sustained improvement in the binocular balance compared with normal controls. In this study, we investigate the cumulative effects of the short-term inverse occlusion in adults and old children with amblyopia. This is a prospective cohort study of 18 amblyopes (10-35 years old; 2 with strabismus) who have been subjected to 2 hours/day of inverse occlusion for 2 months. Patients who required refractive correction or whose refractive correction needed updating were given a 2-month period of refractive adaptation. The primary outcome measure was the binocular balance which was measured using a phase combination task; the secondary outcome measures were the best-corrected visual acuity which was measured with a Tumbling E acuity chart and converted to logMAR units and the stereoacuity which was measured with the Random-dot preschool stereogram test. The average binocular gain was 0.11 in terms of the effective contrast ratio (z=−2.344, p=0.019, 2-tailed related samples Wilcoxon Signed Rank Test). The average acuity gain was 0.13 logMAR equivalent (t17=4.76, p<0.001, 2-tailed paired samples t-test). The average stereoacuity gain was 339 arc seconds (z=−2.533, p=0.011). Based on more recent research concerning adult ocular dominance plasticity, we conclude that inverse occlusion in adults and old children with amblyopia does produce long-term gains to binocular balance and that acuity and stereopsis can improve in some subjects.

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Investigation of the Effect of Regular Exercise on Some Motoric Features in Children in the 7–12 Age Group

Journal of Education and Learning ◽

10.5539/jel.v8n3p81 ◽

2019 ◽

Vol 8 (3) ◽

pp. 81

Author(s):

Kürşat Hazar

Keyword(s):

Body Weight ◽

Exercise Program ◽

T Test ◽

The Body ◽

Control Group ◽

Regular Exercise ◽

Age Group ◽

Paired Samples ◽

Explosive Force ◽

The Difference

The aim of this study was to investigate the effects of regular exercise on some motoric features in 7–12-year-old boys and girls in 8 weeks period of time. To the study 66 boys with an average age of 8, ± 7963, as 54 of them in research and 12 of them in control groups; 66 girls, 36 of them as research and 30 as control group, a total of 132 individuals who joins summer sports schools within the body of Muğla Sıtkı Koçman Directorate of Health, Culture and Sports with no health problems participated in the study. Two tests were applied to the children before and after the exercise program. Height, body weight, balance, flexibility, bounce force, explosive force, sit-up and 20m velocity measurements were performed to the participants. SPSS 21.00 package program was used for statistical evaluation of the obtained data. For paired comparisons, Paired Samples t test was applied to determine the effect of the independent variable on the dependent variable. In order to determine the difference between the groups, Independent samples t test was applied and the level of significance was set at p <0.05 and 0.01. In the study, statistically significant differences were found in favor of the research group between the first and second measurement results of height, body weight, balance, flexibility, bounce force, explosive force, sit-up and velocity (p <0.05, p <0.01). As a result, in the light of the data obtained from the comparisons between the groups, it was determined that the regularly performed exercises positively affect the body weight, static balance, flexibility, bounce force, explosive force, sit-up and velocity values in 7–12 age group boys and girls.

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Clinical and laboratory findings associated with naturally occurring babesiosis in dromedary camels

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/bvip-2014-0034 ◽

2014 ◽

Vol 58 (2) ◽

pp. 229-233 ◽

Cited By ~ 10

Author(s):

Ayman A. Swelum ◽

Alaa B. Ismael ◽

Ahmed F. Khalaf ◽

Mohamed A. Abouheif

Keyword(s):

Blood Cell ◽

Control Group ◽

Iron Level ◽

Dromedary Camels ◽

Laboratory Findings ◽

Naturally Occurring ◽

Blood Smears ◽

Mean Corpuscular Haemoglobin ◽

Biochemical Analyses ◽

Wbc Count

Abstract Clinical, haematological, and biochemical changes induced by naturally occurring babesiosis in dromedary camels were described. Of 258 dromedary camels studied, 34 camels suffered from fever, appetite loss, weakness, depression, and reluctant movement; abortion and/or infertility were also observed. Parasitological blood examinations were performed using Giemsastained blood smears. The clinically affected animals were diagnosed with babesiosis, with 13.17% overall morbidity. Camels that suffered from babesiosis were subjected to haematological and biochemical analyses and the affected group was compared with a control group containing 34 healthy camels. The affected animals showed a highly significant (P<0.001) reduction of the total red blood cell (RBC) count, haemoglobin (HGB) concentration, and mean corpuscular volume (MCV) as well as a highly significant reduction (P<0.01) of haematocrit (HCT) and a significant reduction of (P<0.05) mean corpuscular haemoglobin (MCH). Additional, highly significant increases (P<0.01) in white blood cell (WBC) count and plateletcrit (PCT) percentage were detected. However, other haematological parameters were not significantly altered. There was a very significant reduction (P<0.001) of the blood iron level and a very significant increase (P<0.001) in blood urea nitrogen (BUN) and lactate dehydrogenase (LDH) in the affected camels. Additionally, significant increases in total protein, albumin, γ-glutamyltransferase (GGT), aspartate aminotransferase (AST), and total bilirubin were observed in the affected camels. It was concluded that babesiosis highly affects the haematobiochemical parameters of dromedary camels, including the liver, kidney, and muscle functions. These results represent novel findings concerning natural babesiosis in camels.

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Effects of Rapid Maxillary Expansion on Head Posture, Postural Stability, and Fall Risk

APOS Trends in Orthodontics ◽

10.4103/apos.apos_64_17 ◽

2017 ◽

Vol 7 ◽

pp. 175-180

Author(s):

Fatih Celebi ◽

Feyza Hologlu ◽

Sibel Akbulut ◽

Ali Altug Bicakci

Keyword(s):

Fall Risk ◽

Postural Stability ◽

Head Position ◽

Rapid Maxillary Expansion ◽

Risk Scores ◽

Rank Test ◽

Control Group ◽

Maxillary Expansion ◽

Head Posture ◽

Paired Samples

Objective The aim of this study was to investigate the effects of rapid maxillary expansion (RME) on head posture, postural stability, and fall risk. Materials and Methods A sample of 51 adolescent patients was randomly divided into two groups. In the first group, which consisted of 28 patients (15 females and 13 males), RME was performed as a part of routine orthodontic treatment. The remaining 23 individuals (12 females and 11 males) served as the control group. Lateral cephalometric radiographs taken in natural head position, postural stability, and fall risk scores were obtained during the first visit. They were repeated on average 3.8 months and 3.5 months later for the study and control groups, respectively. The changes were analyzed using the Wilcoxon signed-rank test, paired samples t-test, Mann–Whitney U-test, and independent samples t-test. Results As a result of RME, a statistically significant decrease was detected in the fall risk score (P < 0.05) in the study group, while the head position and postural stability remained unchanged. For the control group, no significant changes were observed in all measurements. Conclusions The result of the present study suggests that RME has a capacity of improving fall risk.

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The effect of ketamine on preventing postpartum depression

Journal of Medicine and Life ◽

10.25122/jml-2020-0116 ◽

2021 ◽

Vol 14 (1) ◽

pp. 87-92

Author(s):

Mina Alipoor ◽

◽

Marzeyeh Loripoor ◽

Majid Kazemi ◽

Farshid Farahbakhsh ◽

...

Keyword(s):

Body Weight ◽

Postpartum Depression ◽

Repeated Measures ◽

Intervention Group ◽

Depression Scale ◽

Caesarian Section ◽

Depression Score ◽

Control Group ◽

Chi Square ◽

Double Blind

Postpartum depression is a common disabling psychosocial disorder that could have adverse effects on the life of the mother, infant, and family. The present study was conducted to evaluate the effect of ketamine on preventing postpartum depression in women undergoing caesarian sections considering the relatively known positive effect of ketamine on major depression. The present double-blind, randomized clinical trial was conducted on 134 women undergoing scheduled caesarian sections. Participants were randomly allocated into two groups of control and intervention. To induce anesthesia, 1–2 mg/kg of body weight of Nesdonal and 0.5 mg/kg of body weight of ketamine were used in the intervention group, while only 3–5 mg/kg of body weight Nesdonal was administered in the control group. Data were gathered using the Edinburgh Postnatal Depression Scale (EPDS) in three stages: before the caesarian section and two and four weeks after the caesarian section. Data were analyzed using variance analysis with repeated measures and the Chi-square test. Results of the present study showed that the mean (± standard deviation) of the depression score in the intervention and control groups were 13.78±3.87 and 13.79±4.78(p = 0.98) before the caesarian section, 11.82±3.41 and 14.34±4.29 (p < 0.001) two weeks after and 10.84±3.48 and 13.09±3.79 (p = 0.001) four weeks after the caesarian section, respectively. Using ketamine in the induction of general anesthesia could be effective in preventing postpartum depression. However, further studies are required to strengthen these findings.

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Pengaruh Pemijatan terhadap Pertumbuhan dan Perkembangan Bayi Umur 3 – 4 Bulan di Wilayah Kerja Puskesmas Kanjilo Kecamatan Barombong Kabupaten Gowa

UMI Medical Journal ◽

10.33096/umj.v1i1.3 ◽

2019 ◽

Vol 1 (1) ◽

pp. 1-14

Author(s):

Anna Sari Dewi

Keyword(s):

Body Weight ◽

Weight Gain ◽

Body Weight Gain ◽

Research Result ◽

The Body ◽

Rank Test ◽

Control Group ◽

Significance Level ◽

Development Indicator ◽

And Control

The research aimed at investigating whether there was the massage effect on the growth and development of the infants of 3 – 4 months old in the working area of PHC Kanjilo, Barombong District, Gowa Regency. This was a quasy experiment research with the pretest-posttest method on the treatment and control groups. Sample consisted of 38 subjects who were selected by the purposive sampling technique. The samples were divided into 2 group, namely the treatment group ( n=19) and control group (n=19) by matching the same age and sex in the two groups. The variables measured were the body weight gain, height gain, development indicator change. The research used the statistic test of Wilcoxon signed rank test, Mann Whitney U test, McNemar test with the significance level of 0.05. The research result indicates that after the massage has been carried out for 30 days, there is the significant change particularly on the body weight gain variable with the value of p = 0,008 (<0.05) and the development indicator change variable with the value of p = 0.05. While for the height, the change is not significant between the infants who are given the massage and are not given the massage with the value of p = 0,091 (> 0.05), likewise, the development indicator change is not significant on the infants who are not massaged (p=0,317).

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Improving patient satisfaction: the virtual breakthrough series collaborative

International Journal of Health Care Quality Assurance ◽

10.1108/ijhcqa-01-2018-0014 ◽

2019 ◽

Vol 32 (1) ◽

pp. 296-306

Author(s):

Made Indra Wijaya ◽

Abd Rahim Mohamad ◽

Muhammad Hafizurrachman

Keyword(s):

Patient Satisfaction ◽

Continuous Improvement ◽

Repeated Measures ◽

Rank Test ◽

Control Group ◽

Feedback Form ◽

Content Type ◽

Friedman's Test ◽

Breakthrough Series Collaborative ◽

Breakthrough Series

Purpose The purpose of this paper is to improve the Siloam Hospitals’ (SHs) patient satisfaction index (PSI) and overcome Indonesia’s geographical barriers. Design/methodology/approach The topic was selected for reasons guided by the Institute of Healthcare Improvement virtual breakthrough series collaborative (VBSC). Subject matter experts came from existing global quality development in collaboration with sales and marketing, and talent management agencies/departments. Patient satisfaction (PS) was measured using the SH Customer Feedback Form. Data were analysed using Friedman’s test. Findings The in-patient (IP) department PSI repeated measures comparison during VBSC, performed using Friedman’s test, showed a statistically significant increase in the PSI, χ2 = 44.00, p<0.001. Post hoc analysis with Wilcoxon signed-rank test was conducted with a Bonferroni correction applied, which resulted in a significant increase between the baseline and action phases (Z=3.317, p=0.003) between the baseline and continuous improvement phases (Z=6.633, p<0.001), and between the action and continuous improvement phases (Z=3.317, p=0.003), suggesting that IP PSI was continuously increasing during all VBSC phases. Like IP PSI, the out-patient department PSI was also continuously increasing during all VBSC phases. Research limitations/implications The VBSC was not implemented using a control group. Factors other than the VBSC may have contributed to increased PS. Practical implications The VBSC was conducted using virtual telecommunication. Although conventional breakthrough series might result in better cohesiveness and commitment, Indonesian geographical barriers forced an alternative strategy, which is much more cost-effective. Originality/value The VBSC, designed to improve PS, has never been implemented in any Indonesian private hospital group. Other hospital groups might also appreciate knowing about the VBSC to improve their PSI.

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