Effect of resveratrol on tubular damage and interstitial fibrosis in kidneys of rats exposed to cigarette smoke

2009 ◽  
Vol 25 (8) ◽  
pp. 539-544 ◽  
Author(s):  
Meltem Kuruş ◽  
Murat Ugras ◽  
Mukaddes Esrefoglu
Keyword(s):  
Cigarette Smoke ◽  
Interstitial Fibrosis ◽  
Periodic Acid ◽  
Control Group ◽  
Albino Rats ◽  
Tubular Damage ◽  
Tubular Atrophy ◽  
Hydropic Degeneration ◽  
Group 4 ◽  
Group 2

The aim of this study was to evaluate the effect of resveratrol on kidney tissue of rats exposed to cigarette smoke. Forty adult male Wistar Albino rats were divided into four groups. Animals in group 1 was the control group. For 6 weeks, group 2 was exposed to cigarette smoke; group 3 received daily intraperitoneal injections of resveratrol (10 mg/kg/d); and group 4 was exposed to both cigarette smoke and intraperitoneal resveratrol. All rats were sacrificed with cervical dislocation. The kidney tissues were obtained, fixed in Bouin’s fixative and embeded in paraffin blocks. Samples were sectioned to 4-5 microns thickness, stained with hematoxylin/eosin (H/E), Masson’s trichromic, periodic acid-schiff (PAS) and were examined by light microscopy for tubular injury and interstitial fibrosis. Results were compared by non-parametric tests. Hydropic degeneration, tubular atrophy, tubulo-interstitial fibrosis, interstitial cell infiltration, vacuolar degeneration and desquamation were prominent in group 2. In group 4, hydropic degeneration, epithelial cell vacuolization and desquamation was not observed, but occasional tubular atrophy and dilation were observed. Our study suggests that, some morphological alterations in the rat kidney, due to cigarette smoke may be prevented by resveratrol.

scholarly journals Cross Talk Between Down Regulation of Steroidogenic Genes Expression, Oxidative and Apoptotic Biomarkers in Testes Induced by Administration of Tramadol and Boldenone and Their Combination in Male Albino Rats

2021 ◽  
Author(s):  
Gihan F. Asaad ◽  
Noha Mowaad ◽  
Marwa E.A. El-Shamarka ◽  
Sahar Khalil
Keyword(s):  
Control Group ◽  
Albino Rats ◽  
Group 4 ◽  
Vital Functions ◽  
Concurrent Use ◽  
Genes Expression ◽  
Group 3 ◽  
Group 2 ◽  
Oxidative Biomarkers ◽  
Reproductive Gland

Abstract BackgroundThe testis is the male reproductive gland or gonad having two vital functions - to produce both sperm and androgens, primarily testosterone.PurposeThe study aimed to investigate the effect of tramadol and boldenone injected alone or in combinatio for 2 months in rats on testicular function.MethodsGroup 1; normal control, Group 2; tramadol Hcl (TRAM) (20 mg/kg bwt.) (i.p). Group 3; boldenone undecylenate (BOLD) (5 mg/kg bwt) (i.m). Group 4; combination of TRAM (20 mg/kg bwt.) and BOLD (5 mg/kg), respectively for 2 months.ResultsTRAM and BOLD alone and in combination rats showed deteriorated testicular functions, lowered serum steroid levels (FSH, LH and testesterone), elevation in oxidative biomarkers (MDA & NO) and reduction in GSH and SOD, downregulation of StaR and HSD17B3 as well as assessment of testicular histopathological using H&E staining, PAS stain for histochemical assessment of polysaccharides and glycoproteins in the testes and Masson trichrome stain to assess the changes in the collagen fibers.ConclusionThe study illuminated the hazard of administration of these drugs for a long period as well as the prominent deleterious effects reported on concurrent use of both drugs.

The Inhibitory Ability of Orobanche Extract on Calcium Oxalate Lithiasis in Male Albino Rats

2020 ◽  
Author(s):  
A. M. Kamal ◽  
M. S. Taha
Keyword(s):  
Treatment Group ◽  
Ethylene Glycol ◽  
Kidney Tissue ◽  
Inhibitory Effect ◽  
Control Group ◽  
Albino Rats ◽  
Potential Candidate ◽  
Group 4 ◽  
Group 5 ◽  
Group 2

The present study aimed to evaluate the inhibitory effect of Orobanche extract in ethylene glycol induced nephrolithiasis. Thirty male albino rats were divided into five groups each group contains 6 animals, group (1) control group, group (2) animals were supplied with 0.75% ethylene glycol in drinking water, group (3) animals were administrated Orobanche extract 3g/kg orally, group (4) animals were administrated Cystone 500 mg/kg in addition to 0.75% ethylene glycol, group (5) animals were administrated Orobanche extract 3g/kg orally in addition to 0.75% ethylene glycol the experiment continued for 28 days. Serum and the kidney homogenates were analyzed for various biochemical parameters and urine was examined microscopically for crystals. Orobanche treatment group and Cystone treatment group significantly decreased phosphorus, Calcium and Oxalate in kidney tissue of nephrolithiasis rats and significantly decreased kidney and liver marker in serum of nephrolithiasis rats. Conclusion this result revealed that Orobanche extract could be a potential candidate for phytotherapy against nephrolithiasis.

scholarly journals The Effects of Pomegranate and Carvacrol on Methotrexate-Induced Bone Marrow Toxicity in Rats

2014 ◽  
Vol 37 (2) ◽  
pp. 93 ◽  
Author(s):  
Velat Şen ◽  
Mehtap Bozkurt ◽  
Sevda Söker ◽  
Aydın Ece ◽  
Ali Güneş ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Hematological Parameters ◽  
Control Group ◽  
Albino Rats ◽  
Bone Marrow Toxicity ◽  
Group 4 ◽  
Bone Marrow Damage ◽  
Group 2 ◽  
Wistar Albino Rats

Purpose: The aim of this study was to evaluate the effects of pomegranate (PMG) extract and carvacrol (CARV) on methotrexate (MTX)-induced oxidative stress and bone marrow toxicity. Methods: Wistar albino rats (32 rats) were divided into four groups (n=8): Group 1 was control; Group 2 was given a single intraperitoneal injection of methotrexate (20 mg/kg); Group 3 was treated with carvacrol (73 mg/kg i.p.) one day before MTX (20 mg/kg i.p.) injection; and, Group 4 received a single dose of MTX (20 mg/kg i.p) while PMG was administered orally for seven days at 225 mg/kg. After animals were euthanized, blood samples were taken to evaluate hematological parameters and oxidative stress. In addition, the femur was cropped and bone marrow was extracted for examination. Results: White blood cell count, hemoglobin, hematocrit and platelet count were found to be decreased in the MTX group, but these changes were prevented in the groups that received CARV and PMG. Furthermore, decreased bone marrow cellularity was found in the groups treated with MTX, whereas the PMG and CARV groups had cellularity similar to controls. Strikingly, oxidative stress increased in the MTX group, but was ultimately decreased in the rats that received the antioxidants PMG and CARV. Conclusion: Carvacrol and PMG were found to be protective against methotrexate-induced oxidative bone marrow damage. Use of these antioxidants, in combination with chemotherapeutics, may help to reduce some adverse effects of methotrexate.

NARINGENIN MITIGATES BERYLLIUM INDUCED BEHAVIORAL ALTERATIONS IN RATS

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
KOMAL SINGH SUMAN ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Therapeutic Potential ◽  
Control Group ◽  
Albino Rats ◽  
Behavioral Alterations ◽  
Dark Chamber ◽  
Group 4 ◽  
Therapeutic Choice ◽  
Plus Maze ◽  
Group 2 ◽  
First Time

Beryllium induced neurotoxicity and therapeutic potential of naringenin had been explored for the first time in rats. For this purpose, 30 female albino rats were divided into six groups. Group 1 served as control, group 2 was naringenin per se and rest of the four groups were exposed to beryllium nitrate (1mg/kg, i.p.) daily for 28 days. Naringenin was orally administered in group 4, 5 and 6 at different doses (10, 20 and 30 mg/kg) for 5 days after 28 days of neurotoxicity. All the animals were subjected to elevate plus maze, light dark chamber and rotarod experiments. Beryllium exposure decreased body weight, time spent and number of entries in open arm, increased time spent in close arms as compared to control in elevated plus maze whereas decreased % time spent in bright arena, number of entries in bright arena and increased % time spent in the dark arena in light and dark chamber; decreased motar coordination and balance skills on rotarod. Naringenin showed therapeutic potential and brought the studied variables more towards control at 20 mg/kg dose. It can thus, be concluded that naringenin may be an agent of therapeutic choice in case of beryllium induced behavioral alterations.

scholarly journals The Effects of Curcumin on Wound Healing in a Rat Model of Nasal Mucosal Trauma

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Gokhan Emiroglu ◽  
Zerrin Ozergin Coskun ◽  
Yildiray Kalkan ◽  
Ozlem Celebi Erdivanli ◽  
Levent Tumkaya ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dimethyl Sulfoxide ◽  
Control Group ◽  
Albino Rats ◽  
Sprague Dawley ◽  
Group 4 ◽  
Leukocytic Infiltration ◽  
Sham Control Group ◽  
Group 2 ◽  
Group 1

We explored the effects of topical curcumin on the healing of nasal mucosal wounds. A total of 32 Sprague-Dawley Albino rats were randomized in equal numbers into four groups, and unilateral nasal wounds were created using an interdental brush. Group 1 (the sham-control group) contained untreated rats with traumatized right-side nasal cavities; Group 2 and 3 rats were similarly traumatized and treated with topical curcumin (5 and 10 mg/mL) dissolved in dimethyl sulfoxide daily for 7 days after trauma; Group 4 rats were treated with topical dimethyl sulfoxide only. All rats were decapitated on day 15 and the healing sites evaluated by blinded observers in terms of the presence of cellular hyperplasia, goblet cell hypertrophy and degeneration, leucocytic infiltration, ciliary loss and degeneration, edema, and vascular dilation. On histopathological evaluation, all of cellular hyperplasia, leukocytic infiltration, and edema were significantly reduced in Group 3 compared with Group 1 (p=0.001, p=0.004, and p=0.008, resp.). Thus, curcumin reduced the inflammatory response and significantly accelerated wound healing.

scholarly journals QUERCETIN NANOPARTICLES REPRESSED LIVER AND BRAIN TOXICITIES INDUCED BY TARTRAZINE IN RATS

2018 ◽  
Vol 8 (5) ◽  
pp. 230-240
Author(s):  
G. Mohamed Eman ◽  
M. Abo-laila Ibrahim ◽  
A.M. Hendawy Hassan ◽  
Mohammad F Ebtehal
Keyword(s):  
Brain Injuries ◽  
Histopathological Examination ◽  
Blood Parameters ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Glutathione Peroxidases ◽  
Group 4 ◽  
Gaba Content ◽  
Group 2

Tartrazine (TZ) is an azobenzene artificial yellow dye for foods, drugs, and cosmetics. Quercetin is member of the flavonoid family. The current study investigated the protective effect of quercetin nanoparticles (QNPs) against TZ. Male albino rats were divided into group1:  control, group 2: TZ (50 mg/kg), group 3: QNPs (5 mg/kg), and group 4: QNPs+TZ. The results of the present study revealed that, the oral administration of TZ dye caused a significantly increased liver biomarkers. Also, a marked effect on lipid profile and blood parameters were shown. In addition TZ induced an elevation in the examined oxidative stress biomarkers and decrease in glutathione peroxidases and acetylcholine esterase. TZ diminished exploration and rearing in open field test as well as elevation in GABA content and apoptosis as well as changes in tissues by histopathological examination. In fact, the results showed good influence of QNPs in improving injuries associated with TZ administration. Keywords:  Tartrazine Dye, Quercetin Nanoparticles, Liver-Brain injuries.

scholarly journals The Curative Effect of Berberine Nanoparticles and Cisplatin Combination Therapies Against Hepatocarcinogenesis-Induced By N-Nitroso-Diethylamine In Male Rat

2018 ◽  
Vol 11 (1) ◽  
pp. 2180-2200
Author(s):  
Nema Abdelhameed Mohamed ◽  
Awatef Mohamed Ali ◽  
Doaa Ahmed Ghareeb ◽  
Adham Rashed Mohamed ◽  
Yasmin Mohamed Elmokhtar
Keyword(s):  
Gene Expression ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Albino Rats ◽  
Group 4 ◽  
Ccl4 Treatment ◽  
Group 5 ◽  
Group 2 ◽  
Wistar Albino Rats

This study aimed to investigate whether berberine nanoparticles (BBR-NPs) and/or cisplatin supplementation could prevent hepatocarcinogenesis-induced by N-nitroso-diethylamine (DENA) in male rats. Male Wistar albino rats were divided into five groups; Group 1: Control; Group 2: DENA-CCl4; Group 3: DENA-CCl4+Cisplatin; Group 4: DENA-CCl4+BBR-NPs; Group 5: DENA-CCl4+Cisplatin+BBR-NPs. DENA-CCl4 significantly increase AST, ALT, ALP, LDH, GGT, AFP activities and total bilirubin, while, 5, NT,  total protein and albumin decreased. DENA-CCl4 treatment caused increment in MDA levels and reduction in SOD, CAT, GPx and GSH in liver tissues. Moreover, DENA-CCl4 increase the gene expression of ADAM17 and TNF-α however,  P53 was declined. In addition, DENA-CCl4 caused severe histopathological lesions in the liver tissue. Interestingly, administration of berberine nanoparticles alone or in combination with cisplatin improves the hepatocarcinogenesis induced by DENA-CCl4 on the physiological, biochemical, molecular and histological levels by decreasing oxidative stress and preserving gene expression of ADAM17, TNF-α and P53. The present findings suggest that BBR-NPs with cisplatin might offer a promising strategy for the prevention of liver cancer.

scholarly journals Triazophos induced neuro-splenic toxicity and evaluation of antioxidative potential of aqueous Broccoli extract in Wistar albino rats

2021 ◽  
Vol 13 (2) ◽  
pp. 616-626
Author(s):  
Dharmender Sharma ◽  
Gurinder Kaur Sangha
Keyword(s):  
Histological Study ◽  
Control Group ◽  
Albino Rats ◽  
White Pulp ◽  
Protective Effects ◽  
Glutathione S Transferase ◽  
Group 4 ◽  
Group 5 ◽  
Group 2 ◽  
Wistar Albino Rats

The present investigation was carried out to assess the antioxidative potential of Broccoli sprouts aqueous extract (BE) against triazophos (TZ) induced oxidative stress (OS) in brain and spleen. In the experimental setup, six groups of rats were formed; Control (group 1), BE (group 2), TZ (group 3), and also BE+TZ groups such as BE1 (group 4), BE2 (group 5) and BE3 (group 6) groups. Rats were orally intubated for 30 days as per experimental design. After sacrifice, OS biomarkers viz; catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and lipid peroxidation (LPO) levels were determined in brain and spleen. Acetylcholinesterase (AChE) activity was observed in plasma and brain samples. Histological study of the spleen in TZ rats showed increased thickness of capsule, congestion and hypocellularity in follicles of spleen’s white pulp and the histoarchitecture was restored in TZ+BE group rats. TZ caused degenerative changes in brain histology and rats showed mild congestion along with haemorrhage in the cerebral cortex. Results suggest that TZ exposure is associated with neural toxicity along with altered spleen stress biomarkers, which further corroborates with histopathological findings. It is inferred that BE exerts multi-mechanistic protective effects against TZ induced neuro-splenic toxicity which is attributable to its protective antioxidant actions.

scholarly journals Effects of Energy Drink in Combination with Two Different Brands of Alcohol on Some Biochemical Parameters of Male Albino Rats

2020 ◽  
Vol 5 (9) ◽  
pp. 707-710
Author(s):  
Okuda, A. Frank ◽  
Ehiwario, N. Julius ◽  
Oshilim, O. Anthony
Keyword(s):  
Biochemical Parameters ◽  
Energy Drink ◽  
Control Group ◽  
Albino Rats ◽  
Treatment Groups ◽  
Group 4 ◽  
Significant Difference ◽  
Group 2 ◽  
Biochemical Analyses ◽  
Group 1

This study was carried out to ascertain the effect of energy drink and mixture of energy drink with different brands of alcohol on some biochemical parameters. Sixteen (16) male albino rats weighing 120- 160g were divided into four (4) groups. The groups were divided thus: Group 1: Control- feed and water ad libitum, Group 2: 3.75ml/kg Bullet Energy drink only, Group 3: 3.75ml/kg Bullet energy drink + 1ml/kg of 41% alcohol dry gin and Group 4: 3.75ml/kg Bullet energy drink + 1ml/kg of 5.1% alcoholic beer. The experiment lasted for 28 days after which the animals were fasted for 12 hours before they were sacrificed and their blood collected for biochemical analyses. There was a significant increase in body weight of animals in group 2-4 and also the glucose concentration was elevated. The result presented in table 2 showed that there is a statistically significant difference (p<0.05) in the level of ALT and ALP in treatment groups when compared to the control group (1). Also, elevated level of cholesterol and triglycerides were also observed. From this data, we may conclude that there are high risk of constantly taking energy drink alone or in combination with alcohol.

Dose-Dependent Effect of Short-Term Repeated Exposure to Amitraz on Some Reproductive Parameters in Male Albino Rats

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Seriki Samuel Adinoyi
Keyword(s):  
Sperm Concentration ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Reproductive Parameters ◽  
Albino Rat ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Dose Dependent

Amitraz is a pesticide used in agriculture and public health to control insects, weeds, animals, and vectors of disease. Although the use of pesticides is of benefit in general, abuse of the pesticides is harmful due to their potential toxicity to humans and animals. Present study examined the effect of the pesticide on the male reproductive parameters of the male albino rats. 20 rats were grouped into four. Group 1 was control, Group 2 was given low dose Amitraz, Group 3 was median dose, and Group 4 was high dose. The administration was for a period of 21 days. Results showed that Amitraz has dose dependent toxicity effect on the reproductive parameter of the rat. Amitraz is toxic to the reproductive parameters of the albino rat, and could lower sperm concentration, sperm motility, and sperm morphology. By implication, Amitraz is toxic to the reproductive system and could cause infertility in male.

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