Synthesis and characterization of a polymeric network made of polyethylene glycol and chitosan as a treatment with antibacterial properties for skin wounds

2020 ◽  
Vol 35 (2) ◽  
pp. 274-286
Author(s):  
Claudia M Alonzo-de la Rosa ◽  
Francesco Copes ◽  
Pascale Chevallier ◽  
Jonnathan G Santillán-Benitez ◽  
Georgina Carbajal-de la Torre ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Polyethylene Glycol ◽  
Antibacterial Properties ◽  
The United States ◽  
Dermal Fibroblasts ◽  
Attenuated Total Reflection ◽  
Sequential Method ◽  
Polymeric Networks ◽  
Direct Cytotoxicity

Polyethylene glycol has been widely investigated for wound healing and dressing applications. Despite its advantages (i.e. great biocompatibility), polyethylene glycol lacks antibacterial activity. For this reason, semi-interpenetrated polymeric networks were prepared by combining a chemically cross-linked polyethylene glycol network with chitosan. The aim of this work was to identify the best amount of chitosan able to improve the antibacterial properties against Staphylococcus aureus. Briefly, the networks were synthesized by a sequential method, adding chitosan in different proportion to the polyethylene glycol. The antibacterial activity was tested following the MGA 0100 of the Pharmacopeia of the United States of Mexico. Fourier-transform infrared with attenuated total reflection spectroscopy, scanning electron microscopy and swelling behavior PBS at 37° C and room temperature were also performed to characterize the polymeric networks. The results showed that PC-2% was able to inhibit the bacterial growth of Staphylococcus aureus even more than Fosfomycin antibiotic. The networks showed cylindrical pores of different sizes (50–100 µm). The maximum swelling of all the networks was achieved in PBS at 37°C (>315%). Free hemoglobin and hemolysis assays were also evaluated to know the compatibility with erythrocytes. Human dermal fibroblasts were used to evaluate direct cytotoxicity. Therefore, the produced gels exerted interesting antibacterial activity and showed good biocompatibility properties.

scholarly journals Synthesis of New Oxindoles and Determination of Their Antibacterial Properties

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Pablo E. Romo ◽  
Braulio Insuasty ◽  
Rodrigo Abonia ◽  
María del Pilar Crespo ◽  
Jairo Quiroga
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Neisseria Gonorrhoeae ◽  
Room Temperature ◽  
Antibacterial Properties ◽  
Analytical Techniques ◽  
Toluenesulfonic Acid ◽  
Antistaphylococcal Activity ◽  
New Compounds

A versatile method for the synthesis of new oxindoles was developed by the reaction between substituted isatins and 5-aminopyrazoles. The reaction was carried out at room temperature in ethanol using p-toluenesulfonic acid as the catalyst. The products were obtained with acceptable to excellent yields (44–96%). Structures of the new compounds were unambiguously established by spectroscopic and analytical techniques. The antibacterial activity was determined by microdilution assays. Compounds 3b, 3e, and 3g showed antistaphylococcal activity, particularly compound 3e displayed a potent activity against the vancomycin intermediate Staphylococcus aureus (VISA). Compounds 3i, 3j, and 3o inhibited Neisseria gonorrhoeae growth.

scholarly journals An Investigation on the Electrochemical Behavior and Antibacterial and Cytotoxic Activity of Nickel Trithiocyanurate Complexes

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1782
Author(s):  
Amir M. Ashrafi ◽  
Pavel Kopel ◽  
Lukas Richtera
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Cyclic Voltammetry ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Electrochemical Behavior ◽  
Antibacterial Properties ◽  
Redox Behavior ◽  
Electrochemical Redox

The electrochemical redox behavior of three trinuclear Ni(II) complexes [Ni3(abb)3(H2O)3(µ-ttc)](ClO4)3 (1), [Ni3(tebb)3(H2O)3(µ-ttc)](ClO4)3·H2O (2), and [Ni3(pmdien)3(µ-ttc)](ClO4)3 (3), where abb = 1-(1H-benzimidazol-2-yl)-N-(1H-benzimidazol-2-ylmethyl)methan-amine, ttcH3 = trithiocyanuric acid, tebb = 2-[2-[2-(1H-benzimidazol-2-yl)ethylsulfanyl]ethyl]-1H-benzimidazole, and pmdien = N,N,N′,N″,N″-pentamethyldiethylenetriamine is reported. Cyclic voltammetry (CV) was applied for the study of the electrochemical behavior of these compounds. The results confirmed the presence of ttc and nickel in oxidation state +2 in the synthesized complexes. Moreover, the antibacterial properties and cytotoxic activity of complex 3 was investigated. All the complexes show antibacterial activity against Staphylococcus aureus and Escherichia coli to different extents. The cytotoxic activity of complex 3 and ttcNa3 were studied on G-361, HOS, K-562, and MCF7 cancer cell lines. It was found out that complex 3 possesses the cytotoxic activity against the tested cell lines, whereas ttcNa3 did not show any cytotoxic activity.

Polysaccharide-Based Film of Cassia fistula and it's Antibacterial Activity

2014 ◽  
Vol 875-877 ◽  
pp. 87-90 ◽  
Author(s):  
Chutimon Satirapipathkul ◽  
Tanakan Chatdum
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Water Vapor Permeability ◽  
Inhibition Zone ◽  
Vapor Permeability ◽  
Glycerol Concentration ◽  
Bacterial Strains ◽  
Cassia Fistula

The film-forming potential of isolate of seed polysaccharide fromCassia fistulawas investigated. Increasing the glycerol concentration in the film increased elongation at break, film solubility and water vapor permeability but decreased tensile strength (TS). The film impregnated with the acetone extract ofAtractylodes lanceawas assessed for inhibition ofEscherichia coliandStaphylococcus aureus. The obtained results showed that the films exhibited antibacterial activity against both bacterial strains. Disc-diffusion assay revealed that the film resulted in a larger inhibition zone around the film onStaphylococcus aureusthan it did onEscherichia coliat the same extract concentrations (0.1 to 0.9 wt %). It can be seen that the film has satisfactory physical and antibacterial properties.

Synthesis of Nano-Hydroxyapatite from Snakehead (Channa striata) Fish Bone and its Antibacterial Properties

2020 ◽  
Vol 840 ◽  
pp. 293-299
Author(s):  
Poedji Loekitowati Hariani ◽  
Muryati Muryati ◽  
Muhammad Said ◽  
Salni Salni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Fish Bone ◽  
Molar Ratio ◽  
Temperature Variations ◽  
Gram Negative ◽  
Fish Bones ◽  
Channa Striata

Nano-hydroxyapatite was synthesized by coprecipitation method and tested its antibacterial properties. Nano-hydroxyapatite was synthesized using CaO precursors from snakehead (Channa striata) fish bones and (NH4)2HPO4. The synthesis was carried out with temperature variations of 30, 60, 80, and 100 °C. Antibacterial activity was determined using two types of bacteria, namely gram-positive and gram-negative. The XRD spectra show that the highest peak is hydroxyapatite synthesized at a temperature of 100 °C. Hydroxyapatite produced from various synthesis temperatures has the size of nanoparticles in the range 37.32-49.27 nm. The nano-hydroxyapatite functional groups are characterized using FTIR, the analysis indicate the presence of OH, CO32‒ and PO43‒. The molar ratio Ca/P is obtained of 1.71 approaching theoretical hydroxyapatite of 1.67. The resulted nano-hydroxyapatite has significant antibacterial properties to Escherichia coli and Staphylococcus aureus.

scholarly journals Studies on the antibacterial properties of some basic derivatives of cholane and norcholane

1947 ◽  
Vol 134 (877) ◽  
pp. 538-543 ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Surface Tension ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Lactobacillus Helveticus ◽  
Bacteriostatic Activity ◽  
Gram Positive ◽  
Gram Negative ◽  
Derivatives Of

A series of basic derivatives of cholane and norcholane has been examined for bacteriostatic activity against the Gram-positive Staphylococcus aureus and Lactobacillus helveticus and the Gram-negative Bacillus lactis aerogenes . The basic derivatives, in general, were more highly bacteriostatic against the Gram-positive organisms than against the Gram-negative. Of the compounds studied, the highest antibacterial activity was shown by 3:7:12-trihydroxy-23-guanido-norcholane hydrochloride. No relationship was apparent between the lowering of the surface tension of the medium induced by the compounds and their bacteriostatic activity.

scholarly journals Strategies for penicillin V dendronization with cationic carbosilane dendrons and study of antibacterial properties

2017 ◽  
Vol 95 (9) ◽  
pp. 927-934 ◽  
Author(s):  
Elena Fuentes-Paniagua ◽  
José M. Hernández-Ros ◽  
Juan Soliveri ◽  
José L. Copa-Patiño ◽  
Rafael Gómez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Potassium Salt ◽  
Focal Point ◽  
Antibacterial Properties ◽  
The Other ◽  
Ester Bond ◽  
No Donor ◽  
Penicillin V ◽  
Lactam Ring

Strategies to synthesize a cationic carbosilane dendron containing the antibiotic penicillin V potassium salt (PenVK) at the focal point are discussed. The preparation of such a compound requires the use of systems with no donor atoms such as N or S in their framework, because their presence favours the rupture of the penicillin β-lactam ring. The antibacterial activity of the new dendron containing ammonium groups, at the periphery, and the PenV moiety, at the focal point, against gram-positive Staphylococcus aureus strains was evaluated. These results were compared with those obtained for free PenVK, a related cationic dendron without a penicillin moiety at the focal point, and also compared with an equimolar mixture of this last dendron with free PenV. The data obtained indicate that, on one hand, the conjugation or interaction of PenV with cationic dendrons reduces its activity in comparison with free PenVK. On the other hand, the penicillin dendron is able to release the antibiotic in the presence of esterease, due to the breaking of the ester bond in this derivative.

scholarly journals ANTIBACTERIAL ACTIVITY OF AGASTACHE AURANTIACA ESSENTIAL OILS

2017 ◽  
pp. 63-70 ◽  
Author(s):  
Наталья (Natal'ja) Александровна (Aleksandrovna) Коваленко (Kovalenko) ◽  
Галина (Galina) Николаевна (Nikolaevna) Супиченко (Supichenko) ◽  
Татьяна (Tat'jana) Игоревна (Igorevna) Ахрамович (Ahramovich) ◽  
Анна (Anna) Геннадьевна (Gennad'evna) Шутова (Shutova) ◽  
Виктор (Viktor) Николаевич (Nikolaevich) Леонтьев (Leontiev)
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Gas Liquid Chromatography ◽  
Oil Components ◽  
The Republic ◽  
Main Components

The essential oil obtained by hydrodistillation method from plants of three varieties Agastache aurantiaca, cultivated on the Republic of Belarus were investigated. Some morphobiometric parameters of plants 'Tango', 'Apricot Sprite' and 'Fragrant Delight' and oil yields were determined. Using the technique of gas-liquid chromatography essential oil components were identified and determined. The main components of the essential oil from 'Tango' and 'Fragrant Delight' plants were menton (~ 53 and ~ 65 v/v % respectively) and pulegon (~ 36 and ~ 25 v/v % respectively). The samples of 'Apricot' Sprite' essential oil were rich in isomentone (~ 46 v/v %) and pulegon (~ 41 v/v %). All tested essential oils contained (+)-menton and (+)-pulegon enantiomeric forms only. The antibacterial activity of dimethylsulfoxide and ethanolic essential oil solutions against Staphylococcus aureus, Salmonella alony, Bacillus subtilis, Clostridium sp., Escherichia coli Hfr H, Pseudomonas aeruginosa was proved. Ethanol solutions of essential oils at the concentrations 0.001–0.1 v/v % had significant bactericidal activity. A correlation between the composition of the essential oil and their antibacterial properties was established.

Evaluation of the Glucuronic Acid Production and Antibacterial Properties of Kombucha Black Tea

2019 ◽  
Vol 20 (11) ◽  
pp. 985-990
Author(s):  
Fereshteh Ansari ◽  
Hadi Pourjafar ◽  
Ali Kangari ◽  
Aziz Homayouni
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Salmonella Typhimurium ◽  
Acid Production ◽  
Glucuronic Acid ◽  
Storage Temperature ◽  
Antibacterial Properties ◽  
Lactobacillus Rhamnosus ◽  
Black Tea ◽  
The Difference

Background: Kombucha beverage is considered as a dietary supplement and drinking it strengthens the body’s immune system which prevents diseases. Objective: The purpose of this study was to determine the amount of glucuronic acid and antibacterial activity of Kombucha black tea drink during its production at different storage temperature. Methods: The extent of glucuronic acid at temperatures of 20°C and 30°C was explored by the use of the HPLC system for 21 days. To analyse the antibacterial property, the influence of Kombucha black tea supernatant on the growth of Salmonella typhimurium, Staphylococcus aureus, and Lactobacillus rhamnosus bacteria was examined via the two procedures of the disc and agar well diffusion. Results: The production of glucuronic acid underwent a variation at 20°C from 17.0 mg/L on day 1 to roughly 27.2 mg/L on day 21, and the difference was significant. Furthermore, the quantity of this acid at 30°C increased from 42.2 mg/L on day 1 to 48.0 mg/L on day 21. The amount of glucuronic acid produced at 30°C was significantly greater than that at 20°C (p<0.05). This study indicated that the Kombucha black tea has antibacterial activity against Salmonella typhimurium and Staphylococcus aureus, but not against Lactobacillus rhamnosus. However, there are no statistical differences in antibacterial activity of Kombucha between incubation at 20oC and 30oC (P>0.05). Conclusion: This study offers a perspective on glucuronic acid production (especially in 30°C rather than 20°C) and antibacterial activity of Kombucha black tea beverage.

scholarly journals Synthesis, antibacterial activities, and sustained perfume release properties of optically active5-hydroxy- and 5-acetoxyalkanethioamide analogues

2020 ◽  
Vol 26 (1) ◽  
pp. 84-98
Author(s):  
Yasutaka Shimotori ◽  
Masayuki Hoshi ◽  
Narihito Ogawa ◽  
Tetsuo Miyakoshi ◽  
Taisei Kanamoto
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Sustained Release ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Optically Active ◽  
High Antibacterial Activity ◽  
Subsequent Deacetylation

Abstract5-Acetoxy- and 5-hydroxyalkanethioamide analogues showed high antibacterial activity against Staphylococcus aureus. Antibacterial thioamides were prepared from 5-alkyl-δ-lactones by amidation, thionation, and subsequent deacetylation. Optically active thioamides with 99% diastereomeric excesses were prepared by diastereomeric resolution using Cbz-L-proline as the resolving agent. Antibacterial thioamides were slowly lactonized by a lipase catalyst. Therefore, these thioamides are potential sustained-release perfume compounds having antibacterial properties.

scholarly journals Comparative evaluation of antibacterial activity of silver oxides with different oxygen content

2021 ◽  
pp. 23-28
Author(s):  
E.M. Gordina ◽  
◽  
S.A. Bozhkova ◽  
A.A. Erusin ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Oxygen Content ◽  
Optical Density ◽  
Antibacterial Properties ◽  
Nutrient Broth ◽  
Low Oxygen ◽  
Pure Silver ◽  
And Staphylococcus Aureus ◽  
Density Results

Aim of the study. To study the presence of antibacterial properties in silver oxides with different oxygen content in relation to sensitive and methicillinresistant Staphylococcus aureus strains. Material and Methods. Films of silver and its oxides were coated on paper surface in vacuum via the method of ion-plasma arc sputtering of the metallic target. Antibacterial activity of the obtained samples of silver oxides and pure silver against Staphylococcus aureus АТСС 29213 and Staphylococcus aureus ATCC 43300 was studied applying the diff usion method as well as in liquid nutrient broth with consequent measuring of optical density. Results. As a result of antibacterial activity investigation of the samples obtained via the diff usion method and liquid nutrient broth, it was revealed that all samples possessed antibacterial activity in relation to type strains of S. aureus. Th erewith, the effi cacy of the samples with pure silver was signifi cantly lower than that of silver oxides (Ag0,2O, Ag0,4O, Ag1O, Ag1,5O, Ag2O). It was shown that the activity of silver oxides did not depend on the oxygen concentration in them. Thus, statistically signifi cant diff erences in optical density of the nutrient medium with bacteria and the medium with incubated S. aureus АТСС 29213 (MSSA) and AgxO (р<0.001). Analogical results were obtained aft er studying of infl uence exerted by silver on S. aureus АТСС 43300 (MRSA). Conclusion. All silver oxides samples created were characterised by antibacterial activity against S. aureus. The data obtained point at the prospects in application of silver oxide with low oxygen content for prevention and treatment of Staphylococcus aureus strains infection.

Sign in / Sign up

Export Citation Format

Share Document