Polysaccharide-Based Film of Cassia fistula and it's Antibacterial Activity

The film-forming potential of isolate of seed polysaccharide fromCassia fistulawas investigated. Increasing the glycerol concentration in the film increased elongation at break, film solubility and water vapor permeability but decreased tensile strength (TS). The film impregnated with the acetone extract ofAtractylodes lanceawas assessed for inhibition ofEscherichia coliandStaphylococcus aureus. The obtained results showed that the films exhibited antibacterial activity against both bacterial strains. Disc-diffusion assay revealed that the film resulted in a larger inhibition zone around the film onStaphylococcus aureusthan it did onEscherichia coliat the same extract concentrations (0.1 to 0.9 wt %). It can be seen that the film has satisfactory physical and antibacterial properties.

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Physico-chemical characterization and antibacterial activity of different types of honey tested on strains isolated from hospitalized patients

Journal of Apicultural Science ◽

10.1515/jas-2016-0013 ◽

2016 ◽

Vol 60 (1) ◽

pp. 5-18 ◽

Cited By ~ 1

Author(s):

Lia M. Junie ◽

Mihaela L. Vică ◽

Mirel Glevitzky ◽

Horea V. Matei

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Bacterial Species ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Diffusion Method ◽

Bacterial Strains ◽

Disk Diffusion Method ◽

Physico Chemical

AbstractThe first aim of the study was to compare the antibacterial activity of several types of honey of different origins, against some bacterial resistant strains. The strains had been isolated from patients. The second aim was to discover the correlations between the antibacterial character of honey and the physico-chemical properties of the honey. Ten honey samples (polyfloral, linden, acacia, manna, and sunflower) from the centre of Romania were tested to determine their antibacterial properties against the following bacterial species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica serovar Typhimurium, Bacillus cereus, Bacillus subtilis, and Listeria monocytogenes. Bacterial cultures in nutrient broth and the culture medium Mueller-Hinton agar were used. The susceptibility to antibiotics was performed using the disk diffusion method. All honey samples showed antibacterial activity on the isolated bacterial strains, in particular polyfloral (inhibition zone 13-21 mm in diameter) - because it is the source of several plants, and manna (inhibition zone 13-19.5 mm in diameter), and sunflower (inhibition zone 14-18.5 mm in diameter). Pure honey has a significant antibacterial activity against some bacteria which are resistant to antibiotics. Bacterial strains differed in their sensitivity to honeys. Pseudomonas aeruginosa and Staphylococcus aureus were the most sensitive. The present study revealed that honey antibacterial activity depends on the origin of the honey. We also found that there was a significant correlation between antibacterial activity of honeys and the colour of the honey but not between acidity and pH. The statistical analysis showed that the honey type influences the antibacterial activity (diameter of the bacterial strains inhibition zones).

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Aktivitas Antibakteri Ekstrak Daun Widuri Terhadap Escherichia Coli Dan Staphylococcus Aureus

Jurnal Farmasi Tinctura ◽

10.35316/tinctura.v2i1.1539 ◽

2020 ◽

Vol 2 (1) ◽

pp. 1-9

Author(s):

Siti Zamilatul Azkiyah

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacterial Infections ◽

Leaf Extract ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Inhibition Zone ◽

The Body ◽

And Staphylococcus Aureus

Antibiotics have a very important role in fighting bacterial infections in the body. However, the results given so far have not been optimal in overcoming the problem of bacterial infection. An alternative solution is to develop and study plants that have medicinal potential, for example thistle leaves (Calotrophis gigantean). The purpose of this study was to determine the antibacterial activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus. This research is a laboratory-scale experimental research that is to test the activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus including sample identification, extraction, activity testing by observing the inhibition zone of the two tested bacteria. Based on the results of the study stated that the ethanol extract of 70% Widuri leaves (Calotrophis gigantea) had antibacterial activity and could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The active compounds that are thought to have antibacterial properties in the ethanol extract of Widuri leaves (Calotrophis gigantea) are flavonoids, alkaloids and terpenoids. Keywords: Widuri leaves, Escherichia coli, Staphylococcus aureus, Antibacterial ABSTRAK Pentingnya penanggulangan infeksi bakteri menggunakan antibiotik adalah hal yang lazim dalam terapi farmakologi. Dampak dari pemberian antibiotik masih belum maksimal dalam penanggulangannya terhadap infeksi bakteri. Pemilihan alternatif bahan alam dengan kandungan kimia yang memiliki potensi sebagai antibiotik dapat menjadi pilihan lain dalam mengurangi penggunaan antibiotik sintetik, misalnya tanaman widuri (Calotrophis gigantean). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak daun widuri terhadap Escherichia coli dan Staphylococcus aureus. Desain penelitian meliputi identifikasi sampel, ekstraksi, hingga uji aktivitas dengan mengamati zona hambat dari kedua bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% daun Widuri memiliki aktivitas antibakteri dan dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Senyawa aktif yang diduga bersifat antibakteri pada ekstrak etanol daun Widuri adalah senyawa golongan flavonoid, alkaloid dan terpenoid. Kata Kunci: Daun Widuri, Escherichia coli, Staphylococcus aureus, Antibakteri

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INHIBITORY EFFECT OF ONION (ALLIUM CEPA LINN) AND SUGAR PASTE MIXTURE ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI BY IN VITRO

Public Health of Indonesia ◽

10.36685/phi.v5i4.310 ◽

2019 ◽

Vol 5 (4) ◽

pp. 131-137

Author(s):

Tahiruddin Tahiruddin ◽

Diah Indriastuti

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Allium Cepa ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Control Group ◽

Control Group Design ◽

Onion Extract

Background: Staphylococcus aureus and Escherichia coli are infection agents. The onion is known to have antibacterial properties. Meanwhile, sugar paste is effective to inhibit bacterial growth.Objective: This study aimed to identify the antibacterial activity of onion extract (Allium cepa Linn) and sugar paste mixture on staphylococcus aureus and Escherichia coli by in vitro.Methods: We used a post-test-only control group design with a completely random design. The onion extract with sugar paste mixture was divided into 4 concentrations; 25%, 50%, 75% and 100% with 3 repetitions. The data were analyzed by measuring inhibition zone diameter and tested using the One-Way Analysis of Variance (ANOVA) and Tukey test.Results: Findings showed that on average, the antibacterial activity of onion extract and sugar paste mixture on staphylococcus aureus was 14.57mm, 17.44mm, 18.36mm, and 22.28mm, respectively 25%, 50%, 75%, and 100% concentrations. Meanwhile, on Escherichia coli, it was 17.27mm, 19.67mm, 20.31mm, and 21.62mm.Conclusion: onion extract and sugar paste mixture can inhibit the growth of Staphylococcus aureus and Escherichia coli.

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Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

Jurnal Riset Kimia ◽

10.25077/jrk.v10i2.335 ◽

2019 ◽

Vol 10 (2) ◽

pp. 94-98

Author(s):

Adlis Santoni ◽

Mai Efdi ◽

Akmel Suhada

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Inhibition Zone ◽

Endemic Plant ◽

Dpph Method ◽

Hexane Extracts

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibition zone 10 mm at concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

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FLORAL EXTRACT OF Tibouchina granulosa (DESR.) COGN. PHYTOCHEMISTRY PROSPECTION, AND ANTIBACTERIAL ACTIVITY

Ipê Agronomic Journal ◽

10.37951/2595-6906.2021v5i1.6880 ◽

2021 ◽

Vol 5 (1) ◽

pp. 1-9

Author(s):

Antonio Carlos Pereira de Menezes Filho ◽

Matheus Vinícius Abadia Ventura ◽

Carlos Frederico de Souza Castro

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Floral Organ ◽

Salt Formation ◽

Bacterial Strains ◽

Positive Results

Tibouchina granulosa is a species that blooms annually in several regions of Brazil. This species is still little explored in terms of phytocompounds in all organs of this plant, especially the floral organ. Flowers of T. granulosa were collected in the municipality of Rio Verde, Goiás, Brazil, in 2021. The hydroethanolic floral extract was prepared by maceration and qualitative phytochemical (colorimetric reactions and salt formation) and antibacterial analyzes performed and the results expressed in millimeters of inhibition at different concentrations in mg mL-1. Several phytochemical classes were observed with positive results, especially for alkaloids, phenolics, oxylates, saponins, carbohydrates and tannins. As for the bacterial assay, potential antibacterial activity was observed for all bacterial strains tested, except for Salmonella serovar Thyphymurium and serovar Enteritidis. Expressive inhibitions were observed for Enterococcus faecalis > Pseudomonas aeruginosa > Staphylococcus aureus and Escherichia coli at the highest concentrations between 100-50 mg mL-1. The floral extract of Tibouchina granulosa showed phytotherapeutic potential with the presence of several phytochemical groups and expressive antibacterial activity.

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Synthesis and Antimicrobial Screening of Some Novel 5-Substituted-1,10b-dihydroimidazole[1,2-c]quinazoline Derivatives

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2021.14.2.6 ◽

2021 ◽

Vol 14 (2) ◽

pp. 5406-5411

Author(s):

Haribhai Rabari ◽

Hetal Vankar ◽

Beenkumar Prajapati

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Aspergillus Niger ◽

Antifungal Compound ◽

Bacterial Strains ◽

Standard Drug ◽

Quinazoline Derivatives

The emergence of multidrug microbial resistance is the main challenges that the modern scientists have so far been facing in the recent era. In this respect, new series of drug classes having potential to give antimicrobial effect have been synthesized. A new series of 5- substituted-1,10 b-dihydroimidazole[1,2-c]quinazoline derivatives 8a-e have been synthesized and screened for antibacterial activity and antifungal activity. Synthesized derivatives were characterized by IR, MASS and 1H-NMR spectroscopy. Synthesized compounds show good activity, which was comparable to the standard drug and it can be useful for the further clinical study. Antibacterial activity was evaluated against four different pathogenic bacterial strains like Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudo-monas aeruginosa. Among the screened compounds, 8e show good antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC of 50 and 100 μg/ml respectively. Antifungal activity was evaluated against two strains of fungi. Among the synthesized derivates, compound 8c was emerged out as the potent antifungal compound against Candida albicans and Aspergillus niger with MIC of 25 μg/ml and 75μg/ml respectively. Compound 8e also shows good antifungal activity with MIC of 50 μg/ml against both Candida albicans and Aspergillus niger. The overall results of this study indicated that synthesized quinazoline derivatives had the potential to act as an antibacterial and antifungal agent, hence further investigation is warranted.

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Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

Molecules ◽

10.3390/molecules25173955 ◽

2020 ◽

Vol 25 (17) ◽

pp. 3955 ◽

Cited By ~ 1

Author(s):

Xin Wang ◽

Yi Shen ◽

Kiran Thakur ◽

Jinzhi Han ◽

Jian-Guo Zhang ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Cell Membrane ◽

Bacterial Cell ◽

Tricarboxylic Acid Cycle ◽

Inhibition Zone ◽

Underlying Mechanism ◽

Bacterial Suspension

Though essential oils exhibit antibacterial activity against food pathogens, their underlying mechanism is understudied. We extracted ginger essential oil (GEO) using supercritical CO2 and steam distillation. A chemical composition comparison by GC-MS showed that the main components of the extracted GEOs were zingiberene and α-curcumene. Their antibacterial activity and associated mechanism against Staphylococcus aureus and Escherichia coli were investigated. The diameter of inhibition zone (DIZ) of GEO against S. aureus was 17.1 mm, with a minimum inhibition concentration (MIC) of 1.0 mg/mL, and minimum bactericide concentration (MBC) of 2.0 mg/mL. For E. coli, the DIZ was 12.3 mm with MIC and MBC values of 2.0 mg/mL and 4.0 mg/mL, respectively. The SDS-PAGE analysis revealed that some of the electrophoretic bacterial cell proteins bands disappeared with the increase in GEO concentration. Consequently, the nucleic acids content of bacterial suspension was raised significantly and the metabolic activity of bacteria was markedly decreased. GEO could thus inhibit the expression of some genes linked to bacterial energy metabolism, tricarboxylic acid cycle, cell membrane-related proteins, and DNA metabolism. Our findings speculate the bactericidal effects of GEO primarily through disruption of the bacterial cell membrane indicating its suitability in food perseveration.

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An Investigation on the Electrochemical Behavior and Antibacterial and Cytotoxic Activity of Nickel Trithiocyanurate Complexes

Materials ◽

10.3390/ma13071782 ◽

2020 ◽

Vol 13 (7) ◽

pp. 1782

Author(s):

Amir M. Ashrafi ◽

Pavel Kopel ◽

Lukas Richtera

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Cyclic Voltammetry ◽

Antibacterial Activity ◽

Cytotoxic Activity ◽

Cell Lines ◽

Electrochemical Behavior ◽

Antibacterial Properties ◽

Redox Behavior ◽

Electrochemical Redox

The electrochemical redox behavior of three trinuclear Ni(II) complexes [Ni3(abb)3(H2O)3(µ-ttc)](ClO4)3 (1), [Ni3(tebb)3(H2O)3(µ-ttc)](ClO4)3·H2O (2), and [Ni3(pmdien)3(µ-ttc)](ClO4)3 (3), where abb = 1-(1H-benzimidazol-2-yl)-N-(1H-benzimidazol-2-ylmethyl)methan-amine, ttcH3 = trithiocyanuric acid, tebb = 2-[2-[2-(1H-benzimidazol-2-yl)ethylsulfanyl]ethyl]-1H-benzimidazole, and pmdien = N,N,N′,N″,N″-pentamethyldiethylenetriamine is reported. Cyclic voltammetry (CV) was applied for the study of the electrochemical behavior of these compounds. The results confirmed the presence of ttc and nickel in oxidation state +2 in the synthesized complexes. Moreover, the antibacterial properties and cytotoxic activity of complex 3 was investigated. All the complexes show antibacterial activity against Staphylococcus aureus and Escherichia coli to different extents. The cytotoxic activity of complex 3 and ttcNa3 were studied on G-361, HOS, K-562, and MCF7 cancer cell lines. It was found out that complex 3 possesses the cytotoxic activity against the tested cell lines, whereas ttcNa3 did not show any cytotoxic activity.

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Antibacterial Diterpenes from the Roots of Ceriops tagal

Natural Product Communications ◽

10.1177/1934578x0800300104 ◽

2008 ◽

Vol 3 (1) ◽

pp. 1934578X0800300 ◽

Cited By ~ 1

Author(s):

Musa Chacha ◽

Renameditswe Mapitse ◽

Anthony J. Afolayan ◽

Runner R. T. Majinda

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Cereus ◽

Spectroscopic Data ◽

Dilution Method ◽

Agar Dilution Method ◽

Bacterial Strains ◽

Agar Dilution ◽

Ceriops Tagal

Investigation of the roots of Ceriops tagal led to the isolation of a new isopimarane, together with the known diterpenes isopimar-8(14)-en-15,16-diol and erythroxyl-4(17),15(16)-dien-3-one. The structure of the new compound was identified as isopimar-8(14)-en-16-hydroxy-15-one. These structures were determined from extensive spectroscopic data analysis. The isolates were screened for antibacterial activity using the agar dilution method against ten test bacterial strains ( Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Micrococcus kristinae, Pseudomonas aeruginosa, Salmonella pooni, Serratia marcescens, Staphylococcus aureus, S. epidermidis and Streptococcus pyrogens). Isopimar-8(14)-en-16-hydroxy-15-one exhibited activity, with MIC values of 0.5 mg/mL against Streptococcus pyrogens; 0.25 mg/mL against Salmonella pooni and 0.1 mg/mL against Bacillus cereus, Staphylococcus aureus and Micrococcus kristinae.

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Synthesis of Nano-Hydroxyapatite from Snakehead (Channa striata) Fish Bone and its Antibacterial Properties

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.840.293 ◽

2020 ◽

Vol 840 ◽

pp. 293-299

Author(s):

Poedji Loekitowati Hariani ◽

Muryati Muryati ◽

Muhammad Said ◽

Salni Salni

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Properties ◽

Fish Bone ◽

Molar Ratio ◽

Temperature Variations ◽

Gram Negative ◽

Fish Bones ◽

Channa Striata

Nano-hydroxyapatite was synthesized by coprecipitation method and tested its antibacterial properties. Nano-hydroxyapatite was synthesized using CaO precursors from snakehead (Channa striata) fish bones and (NH4)2HPO4. The synthesis was carried out with temperature variations of 30, 60, 80, and 100 °C. Antibacterial activity was determined using two types of bacteria, namely gram-positive and gram-negative. The XRD spectra show that the highest peak is hydroxyapatite synthesized at a temperature of 100 °C. Hydroxyapatite produced from various synthesis temperatures has the size of nanoparticles in the range 37.32-49.27 nm. The nano-hydroxyapatite functional groups are characterized using FTIR, the analysis indicate the presence of OH, CO32‒ and PO43‒. The molar ratio Ca/P is obtained of 1.71 approaching theoretical hydroxyapatite of 1.67. The resulted nano-hydroxyapatite has significant antibacterial properties to Escherichia coli and Staphylococcus aureus.

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