Suppression of Nε-(Carboxymethyl)Lysine Generation by the Antioxidant N-Acetylcysteine

1999 ◽  
Vol 19 (3) ◽  
pp. 207-210 ◽  
Author(s):  
Masaaki Nakayama ◽  
Giichi Izumi ◽  
Yasushi Nemoto ◽  
Koji Shibata ◽  
Toshio Hasegawa ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Type I Collagen ◽  
Therapeutic Potential ◽  
In Vitro Study ◽  
Type I ◽  
Glycation End Products ◽  
Carboxymethyl Lysine ◽  
Time Courses ◽  
Dialysis Solutions

Objective Accumulated evidence suggests that Nε-(carboxymethyl)lysine (CML), which is a dominant antigen of advanced glycation end-products (AGEs), is generated in the peritoneal cavity of patients undergoing continuous ambulatory peritoneal dialysis (CAPD), and that this process may be involved in the pathophysiology of the peritoneal injury found with CAPD treatment. Since CML is a sequential product of glycation and oxidation processes, CML generation could be suppressed by antioxidants. The aim of this in vitro study was to clarify the effect of N-acetylcysteine (NAC), an antioxidant, on CML generation from proteins under high glucose settings mimicking peritoneal dialysis solutions. Design Test proteins (bovine serum albumin/type I collagen) were incubated continuously for 16 weeks in glucose solutions (200 mmol/L) with or without NAC (2 mmol/L), and the generation time courses (8 and 16 weeks) of CML and furosine (the biomarker of the glycation products of the early Maillard reaction) were determined. Results In both proteins, furosine and CML were progressively generated in accordance with the duration of the incubation period. No apparent differences were found between solutions with and without NAC in furosine levels at the 8th and 16th weeks. However, the generation of CML was lower in the solution with NAC throughout the test periods. Conclusion The results showed that NAC could suppress the generation of CML. This indicates the therapeutic potential of antioxidants for the glycoxidative stress-related peritoneal injury occurring during CAPD.

scholarly journals 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton’s Jelly Mesenchymal Stem Cells

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1448
Author(s):  
Maria Camilla Ciardulli ◽  
Joseph Lovecchio ◽  
Pasqualina Scala ◽  
Erwin Pavel Lamparelli ◽  
Tina Patricia Dale ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Type I Collagen ◽  
In Vitro Study ◽  
Wharton’S Jelly ◽  
Controlled Delivery ◽  
Type I ◽  
Wharton's Jelly

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

scholarly journals Expression of type I collagen in response to Isoniazid exposure is indirect and is facilitated by collateral induction of cytochrome P450 2E1: An in-vitro study

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0236992
Author(s):  
Suman Santra ◽  
Debasree Bishnu ◽  
Gopal Krishna Dhali ◽  
Amal Santra ◽  
Abhijit Chowdhury
Keyword(s):  
Cytochrome P450 ◽  
Type I Collagen ◽  
In Vitro Study ◽  
Vitro Study ◽  
Type I ◽  
Cytochrome P450 2E1

Increase in the Biocompatibility of the Neutralized Chitosan Dermal Scaffold by Reconstruction of Wound Healing Microenvironment: In Vitro Study

2007 ◽  
Vol 342-343 ◽  
pp. 185-188 ◽  
Author(s):  
Yong Ha Youn ◽  
Chun Ho Kim ◽  
Young Ju Choi ◽  
Yong Jae Gin ◽  
Young Sook Son
Keyword(s):  
Wound Healing ◽  
Type I Collagen ◽  
Binding Capacity ◽  
In Vitro Study ◽  
Type I ◽  
Cell Binding ◽  
Chitosan Scaffold ◽  
Dry Process ◽  
Wound Healing Effect

The porous neutralized chitosan scaffold (NCS) was prepared by freeze-dry method. Its poor cell binding capacity was improved approximately five folds by mixing or coating of atelomeric type I collagen. In order to recreate wound-healing microenvironment within the NCS for the better wound healing effect, various concentrations of bFGF and fibronectin (FN) were supplied in the secondary freeze-dry process of the scaffold. NCS+ bFGF and NCS+FN improved the cell binding capacity by four folds and three folds respectively. Therefore supplementation of collagen, b-FGF and/or fibronectin in the NCS can improve the biocompatibility of the chitosanbased scaffold which itself revealed poor cell binding capacity.

scholarly journals Biological efficacy of perpendicular type-I collagen protruded from TiO2-nanotubes

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Chia-Yu Chen ◽  
David. M. Kim ◽  
Cliff Lee ◽  
John Da Silva ◽  
Shigemi Nagai ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Type I Collagen ◽  
Cell Attachment ◽  
In Vitro Study ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Type I ◽  
Nih3t3 Cells ◽  
Biological Efficacy

AbstractThe aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO2 nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly, mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental implants. Ti surface modification was accomplished in two steps. First, TiO2 nanotubes (NT) array was fabricated via anodization. Diameters and depths of NTs were controlled by applied voltage and duration. Subsequently, an electrophoretic fusion (EPF) method was applied to fuse Col-I into nanotube arrays in a perpendicular fashion. Surface wettability was assessed by contact angle measurement. The bioactivity of modified TiO2 surfaces was evaluated in terms of NIH3T3 fibroblast attachment, platelet activation, and collagen extension. Early attachment, aggregation, and activation of platelets as well as release of platelet-related growth factors were demonstrated on NT-EPF surfaces. Platelet-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape. A direct linkage between pseudopod-like processes of fibroblasts to NT-EPF surfaces was observed. Furthermore, the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular fashion. Within the limitation of this in vitro study, the TiO2 nanotube with perpendicular Col-I surface (NT-EPF) promoted better cell attachment, induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal.

Chondrocytes behaviors within type I collagen microspheres and bulk hydrogels: an in vitro study

RSC Advances ◽  
2015 ◽  
Vol 5 (67) ◽  
pp. 54446-54453 ◽  
Author(s):  
Jun Liu ◽  
Hai Lin ◽  
Xiupeng Li ◽  
Yujiang Fan ◽  
Xingdong Zhang
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
In Vitro Study ◽  
Vitro Study ◽  
Type I ◽  
Design And Development ◽  
Proliferation And Differentiation ◽  
Cell Niche

Cell niche, which is considered to be critical to the proliferation and differentiation of cells, is one of the most important aspects for the design and development of ideal scaffolds in tissue engineering.

scholarly journals Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds

2021 ◽  
Vol 22 (21) ◽  
pp. 11474
Author(s):  
Michal Wojcik ◽  
Paulina Kazimierczak ◽  
Vladyslav Vivcharenko ◽  
Malgorzata Koziol ◽  
Agata Przekora
Keyword(s):  
Vitamin C ◽  
Cellular Response ◽  
Type I Collagen ◽  
Therapeutic Potential ◽  
Bioactive Molecules ◽  
Wound Dressings ◽  
Type I ◽  
Burn Wounds ◽  
Dressing Materials

Bioactive dressings are usually produced using natural or synthetic polymers. Recently, special attention has been paid to β-glucans that act as immunomodulators and have pro-healing properties. The aim of this research was to use β-1,3-glucan (curdlan) as a base for the production of bioactive dressing materials (curdlan/agarose and curdlan/chitosan) that were additionally enriched with vitamin C and/or hydrocortisone to improve healing of chronic and burn wounds. The secondary goal of the study was to compressively evaluate biological properties of the biomaterials. In this work, it was shown that vitamin C/hydrocortisone-enriched biomaterials exhibited faster vitamin C release profile than hydrocortisone. Consecutive release of the drugs is a desired phenomenon since it protects wounds against accumulation of high and toxic concentrations of the bioactive molecules. Moreover, biomaterials showed gradual release of low doses of the hydrocortisone, which is beneficial during management of burn wounds with hypergranulation tissue. Among all tested variants of biomaterials, dressing materials enriched with hydrocortisone and a mixture of vitamin C/hydrocortisone showed the best therapeutic potential since they had the ability to significantly reduce MMP-2 synthesis by macrophages and increase TGF-β1 release by skin cells. Moreover, materials containing hydrocortisone and its blend with vitamin C stimulated type I collagen deposition by fibroblasts and positively affected their migration and proliferation. Results of the experiments clearly showed that the developed biomaterials enriched with bioactive agents may be promising dressings for the management of non-healing chronic and burn wounds.

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