scholarly journals Circulating innate lymphoid cell subtypes and altered cytokine profiles following an atherogenic high-fat diet

2021 ◽  
pp. 175342592110536
Author(s):  
Vuyolwethu Mxinwa ◽  
Phiwayinkosi V. Dludla ◽  
Tawanda M. Nyambuya ◽  
Bongani B. Nkambule
Keyword(s):  
Total Cholesterol ◽  
Lymphoid Cell ◽  
High Fat Diet ◽  
Adaptive Immune Response ◽  
Lymphoid Cells ◽  
High Fat ◽  
Innate Lymphoid Cell ◽  
Insulin Levels ◽  
Cytokine Profiles ◽  
Cholesterol Levels

Impaired Glc tolerance and hyperinsulinemia are a hallmark of type 2 diabetes (T2D) and are associated with an altered innate and adaptive immune response. In this study, we used a high-fat diet (HFD)-induced model of pre-diabetes to explore the pathological implications of altered innate lymphoid cell (ILC) profiles in a state of impaired Glc tolerance. Sixteen male C57BL/6 mice were randomized to receive two experimental diets ( n = 8 per group), low-fat (LFD), and HFD for 8–13 wk. We evaluated the levels of circulating innate lymphoid cells and their respective cytokines following HFD-feeding. The HFD group had impaired Glc tolerance, elevated insulin levels, and increased total cholesterol levels. Notably, the levels of circulating ILC1s were elevated following 13 wk of HFD-feeding. Moreover, the levels of TNF-α were decreased, but there were no changes in IFN-γ levels. Lastly, the levels of circulating ILC2s and ILC3s were comparable between the HFD and LFD group. The findings demonstrated that short-term HFD-feeding increases postprandial blood Glc, total cholesterol and insulin levels. However, the metabolic changes did not alter ILC2 and ILC3 levels and their respective cytokine profiles.

Pengaruh Pemberian Terapi Oksigen Hiperbarik Terhadap Kadar Kolesterol Total Pada Tikus Putih (Rattus novergicus) Jantan Galur Wistar Yang Di Induksi Diet Tinggi Lemak

2018 ◽  
Vol 16 (1) ◽  
pp. 1 ◽  
Author(s):  
AZRUL HILDAN SAFRIZAL
Keyword(s):  
Cardiovascular Disease ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
High Fat Diet ◽  
Oxygen Therapy ◽  
Total Cholesterol Level ◽  
High Fat ◽  
Cholesterol Levels

<p>The pattern and lifestyle of today's society with the presence of an interner facility makes people spend more time sitting out than on exercise and increased consumption of high-fat foods may increase the risk of cardiovascular disease. An effective therapy is needed in preventing the occurrence of cardiovascular disease. Hyperbaric oxygen now starts to develop for the treatment of several diseases, which in turn can increase the gene forming antioxidant enzymes and ROS. To determine effect of hyperbaric oxygen therapy on total cholesterol levels of wistar white rats (Rattusnovergicus) induced bye high fat. The study was carried out in an expeative post test only group control of three groups. One group is given standard feed. All groups induced high-fat diet and standard feed. Of the two groups induced, one group was given hyperbaric oxygen therapy with a dose of 3 x 30 minutes for six days on day 7 at a blood test to determine total cholesterol levels<strong>. </strong>One way Anova parametric statistic test showed that p = 0.007 &lt; α proved hypothesis that hyperbaric oxygen therapy giving effect to total cholesterol level of white mice of jantangalurist rings induced by high fat diet. Total cholesterol was significantly different between K (-) and K (+) and between K (-) and P. It was found that hyperbaric oxygen therapy had an effect on total cholesterol level dose of 3x30 minutes for six days.</p>

scholarly journals Anti-hypercholesterolemic effect of Zingiber montanum extract

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1798
Author(s):  
Swandari Paramita ◽  
Meiliati Aminyoto ◽  
Sjarif Ismail ◽  
Enos Tangke Arung
Keyword(s):  
Total Cholesterol ◽  
Plant Extracts ◽  
High Fat Diet ◽  
High Cholesterol ◽  
High Fat ◽  
Group 4 ◽  
Cholesterol Levels ◽  
Group 6 ◽  
Diet Control ◽  
Group 2

Background: High cholesterol levels (hypercholesterolemia) has been recognized to cause various disease, most notably the cardiovascular disease. Unfortunately, most anti-hypercholesterolemic drugs deliver several side effects for patients, by which medicinal plants have begun to attract attention for treating hypercholesterolemia. Among others, Zingiber montanum (J.König) Link ex A.Dietr. has traditionally been taken for treating health problems caused by high cholesterol levels. Hence, this work aimed at investigating anti-hypercholesterolemic effects offered by the plant. Methods: This study was conducted on 30 male Wistar rats. During experiments, the subjects were divided into 6 groups (n=5), i.e. no treatment (Group 1, control); high-fat diet (Group 2, control); high-fat diet with simvastatin (Group 3); high-fat diet with plant extracts (Group 4-6 with 100, 200, and 400 mg/kg BW, respectively). After 4 weeks of treatments, blood samples were collected from each group. Then, plasma concentrations of triglycerides, total cholesterol, high density lipoproteins (HDL), and low density lipoproteins (LDL) were measured. Results: There were significant differences in total cholesterol (p=0.000), LDL (p=0.000) and triglycerides (p=0.001) for Groups 4-6 (high-fat diet treated with different plant extract doses) in comparison with Group 2 (high-fat diet, control). Meanwhile, there were no significant differences in HDL levels (p=0.830) between Group 2 (high-fat diet, control) and other groups. The results also showed significant differences in total cholesterol and LDL for subjects treated with plant extracts (Group 4, 100 mg/kg BW, p=0.000;  Group 5, 200 mg/kg BW, p=0.000; Group 6, 400 mg/kg BW, p=0.000) compared to Group 2 (high-fat diet, control). Then, treatments with 400 mg/kg BW (Group 6) discovered significant reductions in total cholesterol, LDL, and triglycerides (p=0.030). Conclusion: Therefore, Z. montanum has been discovered to deliver anti-hypercholesterolemic effects to experimental subjects, making it potential to act as a natural source of anti-hypercholesterolemic agents.

scholarly journals Anti-hypercholesterolemic effect of Zingiber montanum extract

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1798
Author(s):  
Swandari Paramita ◽  
Meiliati Aminyoto ◽  
Sjarif Ismail ◽  
Enos Tangke Arung
Keyword(s):  
Total Cholesterol ◽  
High Fat Diet ◽  
Diet Group ◽  
High Density Lipoproteins ◽  
Control Group ◽  
High Cholesterol ◽  
High Fat ◽  
Cholesterol Levels ◽  
White Rats ◽  
Diet Control

Background: Hypercholesterolemia, high cholesterol levels in the blood, can contribute to many forms of disease, most notably cardiovascular disease. Anti-hypercholesterolemic agents generally used for those conditions have several side effects for patients. Zingiber montanum, known locally as “bangle”, belongs to the family Zingiberaceae and is a potential plants for alternative anti-hypercholesterolemic agents. This plant, from East Kalimantan, is used in traditional medicine for health problems caused by high cholesterol levels. The aim of this research was to find alternatives to anti-hypercholesterolemic agents, especially from natural sources. Methods: This study was an experimental study using 30 Wistar male white rats. Subjects were randomly divided into 6 groups (n=5): (1) normal control group; (2) high fat diet control group; (3) high fat diet with simvastatin; (4-6) high fat diet with Zingiber montanum extracts 100, 200, and 400 mg/kg. After 4 weeks of treatment, blood was collected from all groups, and plasma concentrations of triglycerides, total cholesterol, high density lipoproteins (HDL), and low density lipoproteins (LDL) were measured. Results: The results showed significant differences in total cholesterol (p=0.000), LDL (p=0.000) and triglycerides (p=0.001) in the high-fat diet group with Z. montanum extract, as compared to the high-fat diet control. Meanwhile, there were no significant differences in HDL levels (p=0.830) between the high-fat diet group and other groups. The results also showed significant differences in total cholesterol and LDLs for rats treated with Z. montanum extract, 100 mg/kg (p=0.000), 200 mg/kg (p=0.000), and 400 mg/kg (p=0.000) compared to the high-fat diet group. The result of Z. montanum 400 mg/kg also showed a significant reduction, not only for total cholesterol and LDLs, but also for triglycerides (p=0.030). Conclusion: It could be concluded that Z. montanum extracts have the potency to be further developed as a new natural source of the anti-hypercholesterolemic agents.

scholarly journals Maternal Quercetin Consumption during Pregnancy May Help Regulate Total Cholesterol/HDL-Cholesterol Ratio without Effect on Cholesterol Levels in Male Progeny Consuming High-Fat Diet

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1242
Author(s):  
Masakatsu Takashima ◽  
Wataru Tanaka ◽  
Hiroki Matsuyama ◽  
Hayato Tajiri ◽  
Hiroyuki Sakakibara
Keyword(s):  
Total Cholesterol ◽  
High Fat Diet ◽  
Control Diet ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Blood Cholesterol ◽  
High Fat ◽  
Cholesterol Ratio ◽  
Male Progeny ◽  
Cholesterol Levels

Quercetin has been shown to have anti-obesity effects, but it is unknown whether these effects can be transmitted from mothers to their progeny. In this study, we investigated whether maternal quercetin consumption during pregnancy has a protective effect on high-fat diet–induced hyper lipid levels and overweight in progeny. Female mice consumed a control diet or a diet containing 1.0% quercetin during breeding. The male progeny were then divided into four groups that were (1) sacrificed at postnatal day 3; (2) born to dams fed the control diet and also fed the control diet (C-C), (3) born to dams fed the control diet and then fed a 30% high-fat diet (C-HF), or (4) born to dams fed the Q-diet and then fed the HF diet (Q-HF). Maternal consumption of quercetin did not affect body weight or blood lipid parameters in either dams or neonates at postnatal day 3. After 13 weeks, the Q-HF group exhibited greater body and liver weights, and higher blood cholesterol levels than the C-HF group. However, the total cholesterol/ high density lipoprotein (HDL)-cholesterol ratios in the Q-HF and C-C groups remained similar. In conclusion, maternal quercetin consumption does not appear to protect the next generation from high-fat diet–induced hyper cholesterol level in the blood and liver, and consequently overweight, but may help regulate the total cholesterol/HDL-cholesterol ratio.

scholarly journals Efek jus belimbing pada kadar Total Kolesterol Rattus norvegicus yang diberi diet tinggi lemak

2019 ◽  
Vol 17 (1) ◽  
pp. 24
Author(s):  
PUTRI DIANA MAULIYAH ULFA ◽  
INDRI NGESTI RAHAYU ◽  
IGM ANTARA HAMBARSIKA
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Total Cholesterol Level ◽  
Standard Diet ◽  
High Fat ◽  
Experimental Animals ◽  
Cholesterol Levels ◽  
Experimental Group

<p>Background: Hypercholesterolemia is a condition in which increased cholesterol concentration in the blood exceeds normal values. Consuming fats and foods rich in cholesterol can increase total cholesterol levels in the blood and cause cholesterol buildup in the arteries. Starfruit (Averrhoa bilimbi L.) has saponin and flavonoid compounds which are expected to play a role in reducing total cholesterol levels. The aim of this study is to determine the effect of star fruit juice (Averrhoa bilimbi L.) to total cholesterol blood levels of male Wistar strain (Rattus norvegicus) with a high-fat diet. Method: Experimental study with post-test only control group design. Twenty-four male white rats (Rattus norvegicus) of the Wistar strain are divided into 3 groups: the experimental group which is given standard diet for 28 days; group of experimental animals who were given a high-fat diet for 28 days; the experimental group treated with a high-fat diet for 28 days and on the 15<sup>th</sup>  until 28<sup>th</sup> days were given starfruit juice (Averrhoa bilimbi L.) with a dose of 4ml/200grwieght/day. On the 29th day, it is measuredtotal cholesterol levels for all groups of experimental animals. Results: The One-Way ANOVA test shows a significant increase in the average of total cholesterol levels (p = 0,009) in group of experimental animals that are given a high-fat diet (x = 60,625± 3,462mg/dL) compared to groups of experimental animals given standard diet (x = 50± 8,468mg/dL). There is a increase in the average in cholesterol level of the experimental group given a high-fat diet and the starfruit juice (Averrhoa bilimbi L.) (x = 65,5 ± 8,960) compared to the mean total cholesterol level of group of animals given a high-fat diet (x = 60,625± 3,462). However, there is no significant increase (p = 0,201). Conclusion: Administration of starfruit juice (Averrhoa bilimbi L.) was not significantly difference in statistics on average of cholesterol level to male wistar rat (Rattus norvegicus) induced by high fat diet</p><p>Keywords: high fat diet, starfruit juice, total cholesterol level</p>

scholarly journals Anti-dyslipidemia Effects of Tart Cherry Dietary Supplementation on Body Weight and Lipid Profiles of Serum in High Fat Diet-Fed Mice

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1192-1192
Author(s):  
Songhee Ahn ◽  
Hyun-Sook !Kim
Keyword(s):  
Body Weight ◽  
Total Cholesterol ◽  
High Fat Diet ◽  
Serum Triglyceride ◽  
Dietary Supplementation ◽  
Control Group ◽  
Tart Cherry ◽  
High Fat ◽  
Cholesterol Levels ◽  
Diet Control

Abstract Objectives The objective of this study was to investigate the anti-dyslipidemia effects of tart cherry supplementation on body weight and lipid profiles of serum in High Fat Diet-Fed Mice. Methods After 2 weeks of adaptation period, forty 7-week-old male C57BL/6J mice were randomly divided into 4 groups (n = 10 per group): normal diet control group (ND), high fat diet control group (HF), HF group fed with 1% tart cherry powder (LC, low dose of cherry), HF group fed with 5% tart cherry powder (HC, high dose of cherry). After 12 weeks of tart cherry dietary supplementation, serum triglyceride (TG), total cholesterol (TC), and high-density lipoprotein cholesterol (HDL-C) were analyzed. Results Final body weight of LC (1%) and HC (5%) was significantly lower than that of HF control group (P &lt; 0.001). Also, body weight gained in LC (1%) and HC (5%) was significantly lower than HF control group (P &lt; 0.001). In serum, triglyceride (TG) and total cholesterol (TC) levels were significantly lower in HC (5%) group compared to HF control group (P &lt; 0.05, P &lt; 0.001, respectively). Serum HDL-cholesterol levels in LC (1%) and HC (5%) groups was significantly higher than HF control group (P &lt; 0.05). Conclusions Tart cherry dietary supplementation may have an anti-dyslipidemia effect in high fat diet-fed mice by lowering body weight gain and modulating serum cholesterol levels. Further analysis of AMPK-regulated fatty acid oxidation biomarkers are under investigation. Funding Sources This study received no external funding.

scholarly journals Anti-Obesity and Hypocholesterolemic Actions of Protamine-Derived Peptide RPR (Arg-Pro-Arg) and Protamine in High-Fat Diet-Induced C57BL/6J Mice

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2501
Author(s):  
Maihemuti Mijiti ◽  
Ryosuke Mori ◽  
Bingyu Huang ◽  
Kenichiro Tsukamoto ◽  
Keisuke Kiriyama ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Fecal Excretion ◽  
Control Group ◽  
High Fat ◽  
Tissue Weight ◽  
Cholesterol Levels ◽  
Adipose Tissue Weight ◽  
Fas Mrna ◽  
Hypocholesterolemic Peptide

Dietary protamine can ameliorate hyperlipidemia; however, the protamine-derived active peptide and its hypolipidemic mechanism of action are unclear. Here, we report the discovery of a novel anti-obesity and hypocholesterolemic peptide, RPR (Arg-Pro-Arg), derived from protamine in mice fed a high-fat diet for 50 days. Serum cholesterol levels were significantly lower in the protamine and RPR groups than in the control group. White adipose tissue weight was significantly decreased in the protamine and RPR groups. The fecal excretion of cholesterol and bile acid was significantly higher in the protamine and RPR groups than in the control group. We also observed a significant decrease in the expression of hepatic SCD1, SREBP1, and adipocyte FAS mRNA, and significantly increased expression of hepatic PPARα and adipocyte PPARγ1 mRNA in the protamine group. These findings demonstrate that the anti-obesity effects of protamine are linked to the upregulation of adipocyte PPARγ1 and hepatic PPARα and the downregulation of hepatic SCD1 via SREBP1 and adipocyte FAS. RPR derived from protamine has a crucial role in the anti-obesity action of protamine by evaluating the effective dose of adipose tissue weight loss.

scholarly journals Effect of Soybean and Soybean Koji on Obesity and Dyslipidemia in Rats Fed a High-Fat Diet: A Comparative Study

2021 ◽  
Vol 18 (11) ◽  
pp. 6032
Author(s):  
Sihoon Park ◽  
Jae-Joon Lee ◽  
Hye-Won Shin ◽  
Sunyoon Jung ◽  
Jung-Heun Ha
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Unsaturated Fatty Acids ◽  
Density Lipoprotein ◽  
Serum Triglyceride ◽  
Lipoprotein Cholesterol ◽  
Health Concern ◽  
High Fat ◽  
Cholesterol Levels

Soybean koji refers to steamed soybeans inoculated with microbial species. Soybean fermentation improves the health benefits of soybeans. Obesity is a serious health concern owing to its increasing incidence rate and high association with other metabolic diseases. Therefore, we investigated the effects of soybean and soybean koji on high-fat diet-induced obesity in rats. Five-week-old male Sprague-Dawley rats were randomly divided into four groups (n = 8/group) as follows: (1) regular diet (RD), (2) high-fat diet (HFD), (3) HFD + steamed soybean (HFD+SS), and (4) HFD + soybean koji (HFD+SK). SK contained more free amino acids and unsaturated fatty acids than SS. In a rat model of obesity, SK consumption significantly alleviated the increase in weight of white adipose tissue and mRNA expression of lipogenic genes, whereas SS consumption did not. Both SS and SK reduced serum triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels, and increased high-density lipoprotein cholesterol levels. SS and SK also inhibited lipid accumulation in the liver and white adipose tissue and reduced adipocyte size. Although both SS and SK could alleviate HFD-induced dyslipidemia, SK has better anti-obesity effects than SS by regulating lipogenesis. Overall, SK is an excellent functional food that may prevent obesity.

scholarly journals Antiobesity and Hypolipidemic Activity of Moringa oleifera Leaves against High Fat Diet-Induced Obesity in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Souravh Bais ◽  
Guru Sewak Singh ◽  
Ramica Sharma
Keyword(s):  
Body Weight ◽  
Body Temperature ◽  
Total Cholesterol ◽  
High Fat Diet ◽  
Moringa Oleifera ◽  
Chronic Administration ◽  
The Body ◽  
Atherogenic Index ◽  
High Fat ◽  
Diet Induced Obesity

In the present study, the methanolic extract of Moringa oleifera leaves (MEMOL) was evaluated for antiobesity activity in rats. The antiobesity potential of MEMOL was studied against high fat diet-induced obesity (HFD) in rats. In this study, chronic administration of HFD in rats produced hypercholesterolemia (116.2 ± 0.27 mg/dL), which led to an increase in the body weight (225 gr), total cholesterol, triglycerides (263.0 ± 4.69 mg/dL), and attenuation in the levels of HDL (34.51 ± 2.20 mg/dL) as well as changes in body temperature of animals. Treatment of obese rats with MEMOL for 49 days resulted in a significant (P<0.001) change in body weight, total cholesterol, triglycerides, and LDL level along with a significant (P<0.001) increase in body temperature as compared to the HFD-induced obesity. MEMOL treated rats also showed a significant decrease in the level of liver biomarkers, organ weight, and blood glucose level. Further, rats treated with MEMOL (200 mg and 400 mg/kg) show reduced atherogenic index (1.7 ± 0.6 and 0.87 ± 0.76). The results indicate that the rats treated with Moringa oleifera (MO) have significantly attenuated the body weight without any change in the feed intake and also elicited significant thermogenic effect and to act as hypolipidemic and thermogenic property in obesity related disorders.

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