scholarly journals Dithiosulfinates and Sulfoxides with Antifungal Activity from Bulbs of Allium sativum L. var. Voghiera

2018 ◽  
Vol 13 (9) ◽  
pp. 1934578X1801300
Author(s):  
Bruna de Falco ◽  
Giuliano Bonanomi ◽  
Virginia Lanzotti
Keyword(s):  
Antimicrobial Activity ◽  
Nmr Spectroscopy ◽  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Trichoderma Harzianum ◽  
Structure Elucidation ◽  
Allium Sativum ◽  
Fungal Species ◽  
Phytochemical Analysis ◽  
Spectroscopic Analyses

A bioassay guided phytochemical analysis of the bulbs of Allium sativum L. var. Voghiera, typical of Voghiera, Ferrara (Italy), allowed the isolation of six new sulfur compounds with dithiosulfinates and sulfoxides functionalities. Structure elucidation of the isolated compounds was carried out by spectroscopic analyses, including NMR spectroscopy and MS spectrometry. Compounds showed significant antimicrobial activity towards two fungal species, the air-borne pathogen Botrytis cinerea and the beneficial fungus Trichoderma harzianum.

Substituted 6,7-dimethoxy-5-oxo-2,3,5,9b-tetrahydrothiazolo[2,3-a]isoindole- 3-1,1-dioxide Derivatives with Antimicrobial Activity and Docking Assisted Prediction of the Mechanism of their Antibacterial and Antifungal Properties

2020 ◽  
Vol 20 (29) ◽  
pp. 2681-2691
Author(s):  
Athina Geronikaki ◽  
Victor Kartsev ◽  
Phaedra Eleftheriou ◽  
Anthi Petrou ◽  
Jasmina Glamočlija ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Drug Targets ◽  
Bacterial Species ◽  
Pharmaceutical Research ◽  
Docking Studies ◽  
Fungal Species ◽  
Docking Analysis ◽  
E Coli ◽  
Antibacterial And Antifungal

Background: Although a great number of the targets of antimicrobial therapy have been achieved, it remains among the first fields of pharmaceutical research, mainly because of the development of resistant strains. Docking analysis may be an important tool in the research for the development of more effective agents against specific drug targets or multi-target agents 1-3. Methods: In the present study, based on docking analysis, ten tetrahydrothiazolo[2,3-a]isoindole derivatives were chosen for the evaluation of the antimicrobial activity. Results: All compounds showed antibacterial activity against eight Gram-positive and Gram-negative bacterial species being, in some cases, more potent than ampicillin and streptomycin against all species. The most sensitive bacteria appeared to be S. aureus and En. Cloacae, while M. flavus, E. coli and P. aeruginosa were the most resistant ones. The compounds were also tested for their antifungal activity against eight fungal species. All compounds exhibited good antifungal activity better than reference drugs bifonazole (1.4 – 41 folds) and ketoconazole (1.1 – 406 folds) against all fungal species. In order to elucidate the mechanism of action, docking studies on different antimicrobial targets were performed. Conclusion: According to docking analysis, the antifungal activity can be explained by the inhibition of the CYP51 enzyme for most compounds with a better correlation of the results obtained for the P.v.c. strain (linear regression between estimated binding Energy and log(1/MIC) with R 2 =0.867 and p=0.000091 or R 2 = 0.924, p= 0.000036, when compound 3 is excluded.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DRIED FLOWERS OF ADENIUM OBESUM

2019 ◽  
pp. 34-36
Author(s):  
SIREESHA KALVA ◽  
RAGHUNANDAN N.
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Inhibition Concentration ◽  
Test Organisms ◽  
Phytochemical Constituents

Objective: To estimate the antimicrobial activity of methanolic extract of Adenium obesum (family Apocyanaceae) in association with phytochemical analysis. Methods: The methanolic extract of the flowers of Adenium obesum was prepared and investigated for phytochemical constituents using standard methods. Antimicrobial activity of methanolic extract Adenium obesum has been carried out against one gram-positive bacteria Staphylococcus aureus and one gram-negative bacteria Escherichia coli and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of methanolic extract was compared with that of standard drug i.e., Ciprofloxacin for antibacterial activity and Fluconazole for antifungal activity. The minimum inhibition concentration was determined by micro dilution method (cup-plate method). Results: The present investigation shows the phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenols, proteins and amino acids, cardiac glycosides, steroids, tannin, and phenolic compounds. The anti-microbial activity of the methanolic extract of the plant showed significant results against all the three of the test organisms in compare to the standard drugs. Conclusion: The present study concluded that methanolic extract of the flowers of adenium obesum proved the existence of phytochemical constituents. The methanolic extract of the was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of antibiotics.

scholarly journals Steroidal Glycosides from the Seeds of Hyoscyamus Niger L. and their Antifungal Activity

2007 ◽  
Vol 2 (1) ◽  
pp. 108-113
Author(s):  
Irina Lunga ◽  
Pavel Chintea ◽  
Stepan Shvets ◽  
Anna Favelb ◽  
Cosimo Pizza
Keyword(s):  
Nmr Spectroscopy ◽  
Antifungal Activity ◽  
Pathogenic Fungi ◽  
Phytochemical Analysis ◽  
Two Dimensional ◽  
Hyoscyamus Niger ◽  
Steroidal Glycosides ◽  
Spirostanol Saponins ◽  
First Time

Phytochemical analysis of the seeds of Hyocyamus niger L. (Solonaceae) resulted in the isolation of six steroidal glycosides, two furostanol (1, 2) and four spirostanol saponins (3, 4, 5, 6), which were found in this plant for the first time. The structures of these compounds were determined by detailed analysis of their spectral data, including two-dimensional NMR spectroscopy and MS spectroscopy. The antifungal activity of a crude steroidal glycoside extract, fractions of spirostanoles and individual glicosides was investigated in vitro against a panel of human pathogenic fungi, yeasts as well as dermatophytes and filamentous species.

scholarly journals Phytochemicals of Apple Pomace as Prospect Bio-Fungicide Agents against Mycotoxigenic Fungal Species—In Vitro Experiments

Toxins ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 361 ◽  
Author(s):  
Marta Oleszek ◽  
Łukasz Pecio ◽  
Solomiia Kozachok ◽  
Żaneta Lachowska-Filipiuk ◽  
Karolina Oszust ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Antioxidant Properties ◽  
Fungal Species ◽  
Apple Pomace ◽  
Quercetin Derivatives ◽  
Spectroscopic Analyses ◽  
Neosartorya Fischeri ◽  
Quercetin Glycosides ◽  
Phytochemical Constituents

The phytochemical constituents of apple waste were established as potential antifungal agents against four crops pathogens, specifically, Botrytis sp., Fusarium oxysporum, Petriella setifera, and Neosartorya fischeri. Crude, purified extracts and fractions of apple pomace were tested in vitro to evaluate their antifungal and antioxidant properties. The phytochemical constituents of the tested materials were mainly represented by phloridzin and quercetin derivatives, as well as previously undescribed in apples, monoterpene–pinnatifidanoside D. Its structure was confirmed by 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopic analyses. The fraction containing quercetin pentosides possessed the highest antioxidant activity, while the strongest antifungal activity was exerted by a fraction containing phloridzin. Sugar moieties differentiated the antifungal activity of quercetin glycosides. Quercetin hexosides possessed stronger antifungal activity than quercetin pentosides.

scholarly journals A COMPARATIVE STUDY ON THE EFFECT OF SOLVENTS ON THE PHYTOCHEMICAL PROFILE AND BIOLOGICAL POTENTIAL OF ORMOCARPUM COCHINCHINENSE AUCT. NON (LOUR.) MERRILL

2016 ◽  
Vol 9 (1) ◽  
pp. 67
Author(s):  
A. Hannah Hepsibah ◽  
G. Jeya Jothi
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Diffusion Method ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Dpph Assay ◽  
Biological Potential ◽  
Phytochemical Profile ◽  
Ethanol Extracts

<p><strong>Objective: </strong>Plants contain different classes of phytochemicals which have different levels of solubility in solvents, based on their polarity. Hence, this study aims to compare the effects of various solvents on the phytochemical profile and biological potential of <em>Ormocarpum cochinchinense</em>. The present study is the first to evaluate the antifungal activity of the plant.<strong></strong></p><p><strong>Methods: </strong>The sequential extraction was carried out using two sets of solvent systems namely hexane, ethyl acetate, ethanol and chloroform, acetone, methanol. The extracts were subjected to standard phytochemical analysis, antimicrobial activity by disc diffusion method against eight bacteria and six fungi and antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay.</p><p><strong>Results: </strong>Varied range of phytochemicals was found in the extracts. Acetone extract was rich in phenolic compounds, whereas terpenoids were extracted only in methanol. Acetone extracts showed highest antibacterial activity with a maximum zone of 14.5 mm against <em>Shigella flexineri</em> whereas ethyl acetate extract showed the best antifungal activity with the highest zone of inhibition of 12 mm against <em>Trichophyton menta agrophytes.</em> Hexane and chloroform extracts did not show any antimicrobial activity. For DPPH assay, the ethanol extracts showed the highest percentage inhibition of 92.87%. <strong></strong></p><p><strong>Conclusion: </strong>The present investigation on the plant <em>O. cochinchinense</em> has proved that the selection of solvent for extraction should be based on the target compounds and their bioactivity and concludes that acetone was the best for the extraction of antibacterial compounds and ethyl acetate for antifungal compounds whereas, ethanol was the best for extracting antioxidant compounds in <em>O. cochinchinense.</em></p>

scholarly journals The efficacy of polyherbal formulation of Moringa oleifera, Viola odorata, Allium sativum against microbes- synergistic effect

2015 ◽  
Vol 3 (04) ◽  
pp. 06-10
Author(s):  
Jagadeesh Singh SD ◽  
Meenakshi P Singh ◽  
Rajesh R
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Synergistic Effect ◽  
Moringa Oleifera ◽  
Allium Sativum ◽  
Salmonella Typhi ◽  
Polyherbal Formulation ◽  
Viola Odorata ◽  
Methanolic Extracts ◽  
High Zone

The purpose of our present study is to test antimicrobial and antifungal activity of polyherbal formulations. The testing of the antimicrobial activity on polyhernal formulation was done against gram positive (Staphylococcus aureus, Bacillus subtilis) and gram negative (Salmonella typhi, Escherichia coli,) bacteria. The testing of antifungal activity was done against Aspergillus niger and Candida albicans with different combinations of polyherbal formulation. The microorganism to be tested was grown in relevant nutritional media. The solvent used for the extraction of Moringa oleifera, Viola odorata, Allium sativum was methanol. These methanolic extracts of chosen plants were further used in specific proportions for the preparation of polyherbal formulation (PF-1, PF-2, and PF-3). The resultant compositions were further used to check the efficacy against selected experimental microorganisms. PF-3 showed the significant high zone of inhibition against all the experimental microorganisms as compared to the other two combinations PF-1 and PF-2. Thus, the result has revealed that the antimicrobial activity is due to the synergistic effect of secondary metabolites present in these selected plants.

scholarly journals Phytochemical Screening and Antimicrobial Activity of Various Extracts of Aerial Parts of Rhanterium epapposum

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1351
Author(s):  
Saravanan Rajendrasozhan ◽  
Hani El Moll ◽  
Mejdi Snoussi ◽  
Ramy M. Romeilah ◽  
Emad A. Shalaby ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phytochemical Content ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Bacteria And Fungi

Rhanterium epapposum, native to the Arabian Peninsula, is traditionally used to cure skin infections. The objective is to screen the phytochemical content and antimicrobial activity of aqueous, methanol and 80% methanol extracts of aerial parts of R. epapposum. The phytochemical screening of aqueous, methanolic, and 80% methanol extracts of R. epapposum was conducted using gas chromatographymass spectrometry. The antimicrobial activities of the extracts were assessed by well diffusion and microdilution methods. Qualitative phytochemical analysis revealed the presence of 2-methoxy-4-vinylphenol in all three extracts, whereas ethanol, 2-methoxy-, acetate; n-hexadecanoic acid; and 2,3-butanediol are present in higher amount exclusively in the methanol, 80% methanol and aqueous extracts of the aerial parts of R. epapposum, respectively. The highest antibacterial activity was shown by the aqueous extract S. aureus, P. aeruginosa, E. cloacae, and K. pneumoniae, methanolic extract against S. aureus, methicillin-resistant S. aureus, and E. coli, and 80% methanol extract against S. epidermidis, and S. paucimobilis. Interestingly, 80% methanol extracts showed the highest antifungal activity against C. albicans, C. guillermondii, C. vaginalis, C. utilis, and C. tropicalis. The aerial parts of R. epapposum showed broad-spread antimicrobial activity against bacteria and fungi. Especially, the 80% methanol extract showed potent antifungal activity against all the tested fungal strains.

scholarly journals Comparison of Antimicrobial Activity ofAllium sativumCloves from China and Taşköprü, Turkey

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Ali Yetgin ◽  
Kerem Canlı ◽  
Ergin Murat Altuner
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

In this study, antimicrobial activities of two different samples ofAllium sativumL. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species ofBacillus,Enterobacter,Enterococcus,Escherichia,Klebsiella,Listeria,Pseudomonas,Salmonella, andStaphylococcuswere used for testing antibacterial activity. In addition, antifungal activity againstCandida albicanswas also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DRIED FLOWERS OF ADENIUM OBESUM

2019 ◽  
pp. 34-36
Author(s):  
SIREESHA KALVA ◽  
RAGHUNANDAN N.
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Inhibition Concentration ◽  
Test Organisms ◽  
Phytochemical Constituents

Objective: To estimate the antimicrobial activity of methanolic extract of Adenium obesum (family Apocyanaceae) in association with phytochemical analysis. Methods: The methanolic extract of the flowers of Adenium obesum was prepared and investigated for phytochemical constituents using standard methods. Antimicrobial activity of methanolic extract Adenium obesum has been carried out against one gram-positive bacteria Staphylococcus aureus and one gram-negative bacteria Escherichia coli and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of methanolic extract was compared with that of standard drug i.e., Ciprofloxacin for antibacterial activity and Fluconazole for antifungal activity. The minimum inhibition concentration was determined by micro dilution method (cup-plate method). Results: The present investigation shows the phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenols, proteins and amino acids, cardiac glycosides, steroids, tannin, and phenolic compounds. The anti-microbial activity of the methanolic extract of the plant showed significant results against all the three of the test organisms in compare to the standard drugs. Conclusion: The present study concluded that methanolic extract of the flowers of adenium obesum proved the existence of phytochemical constituents. The methanolic extract of the was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of antibiotics.

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