scholarly journals Phytochemical Screening and Antimicrobial Activity of Various Extracts of Aerial Parts of Rhanterium epapposum

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1351
Author(s):  
Saravanan Rajendrasozhan ◽  
Hani El Moll ◽  
Mejdi Snoussi ◽  
Ramy M. Romeilah ◽  
Emad A. Shalaby ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phytochemical Content ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Bacteria And Fungi

Rhanterium epapposum, native to the Arabian Peninsula, is traditionally used to cure skin infections. The objective is to screen the phytochemical content and antimicrobial activity of aqueous, methanol and 80% methanol extracts of aerial parts of R. epapposum. The phytochemical screening of aqueous, methanolic, and 80% methanol extracts of R. epapposum was conducted using gas chromatographymass spectrometry. The antimicrobial activities of the extracts were assessed by well diffusion and microdilution methods. Qualitative phytochemical analysis revealed the presence of 2-methoxy-4-vinylphenol in all three extracts, whereas ethanol, 2-methoxy-, acetate; n-hexadecanoic acid; and 2,3-butanediol are present in higher amount exclusively in the methanol, 80% methanol and aqueous extracts of the aerial parts of R. epapposum, respectively. The highest antibacterial activity was shown by the aqueous extract S. aureus, P. aeruginosa, E. cloacae, and K. pneumoniae, methanolic extract against S. aureus, methicillin-resistant S. aureus, and E. coli, and 80% methanol extract against S. epidermidis, and S. paucimobilis. Interestingly, 80% methanol extracts showed the highest antifungal activity against C. albicans, C. guillermondii, C. vaginalis, C. utilis, and C. tropicalis. The aerial parts of R. epapposum showed broad-spread antimicrobial activity against bacteria and fungi. Especially, the 80% methanol extract showed potent antifungal activity against all the tested fungal strains.

Chemical constituents, antimicrobial and antioxidant properties of the aerial parts of Coccinia barteri

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Ethyl Hexadecanoate ◽  
Bacteria And Fungi ◽  
Percentage Abundance

Phytochemical analysis of n-hexane, ethyl acetate and methanol extracts of the aerial parts of Coccinia barteri was carried out. These extracts exhibited satisfactory inhibitory activities against bacteria and fungi strains, which include; Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhii, Klebsiella pneumoniae, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stolonifer. Methanol extract of C. barteri possesses antioxidant activity by scavenging DPPH free radical with IC50 of 187.56 μg/mL, using DPPH antioxidant assay. GC-MS analysis of n-hexane, ethyl acetate and methanol extracts of the plant principally revealed the presence of phytol, ethyl hexadecanoate and clionasterol with their corresponding percentage abundance of 57.75%, 18.33% and 9.79%, respectively.

scholarly journals Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

2008 ◽  
Vol 25 (No. 2) ◽  
pp. 81-89 ◽  
Author(s):  
A. Adiguzel ◽  
H. Ozer ◽  
H. Kilic ◽  
B. Cetin
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Satureja Hortensis ◽  
Bacteria And Fungi

The present work reports the <i>in vitro</i> antimicrobial activities of the essential oil and methanol extract from <i>Satureja hortensis</i> as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), &gamma;-terpinene (18.56%), carvacrol (13.98%), and <i>p</i>-cymene (8.97). The essential oil of <i>Satureja hortensis</i> exhibited the activity against 25 bacteria, 8 fungi, and a yeast, <i>C. albicans</i>; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 &micro;l/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

Preliminary phytochemical screening and antimicrobial activities of plant extract of Elaeocarpus ganitrus Roxb.

2016 ◽  
Vol 5 (09) ◽  
pp. 4885 ◽  
Author(s):  
Khushbu Pandey ◽  
Mahendra Singh* ◽  
Bharat Pandey ◽  
Anshulika Upadhyaya ◽  
Kamal K. Pande
Keyword(s):  
Antimicrobial Activity ◽  
Rhizopus Oryzae ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

The present study was carried out for phytochemical screening of principle bioactive compounds and antimicrobial activity in Elaeocarpus ganitrus Roxb., Phytochemical analysis revealed the presence of saponin, terpenoid, steroid, saponin, flavonoid, tannin and alkaloid. The petroleum, ether, chloroform, methanol, acetone and aqueous extracts were subjected to antimicrobial activity against bacterial strains Staphylococcus aureus, Pseudomonas, E. coli and Bacillus subtilis against anti-fungal strains A.awamori, A.fumigatus, Rhizopus oryzae, Trichoderma viridae and C.oryzae. The antibacterial and antifungal activity was evaluated by disc-diffusion method.

scholarly journals ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF THE LEAF EXTRACTS OF EUCALYPTUS GLOBULUS

2017 ◽  
pp. 85-89
Author(s):  
Subramanian Abirami ◽  
Kannan Nishanthini ◽  
Mani Poonkothai
Keyword(s):  
Antimicrobial Activity ◽  
Functional Groups ◽  
Eucalyptus Globulus ◽  
Acetone Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Ft Ir ◽  
Bacteria And Fungi

Objective: To investigate the antimicrobial activity and phytochemical analysis of Eucalyptus globulus leaves.Methods: The antimicrobial activity of the leaves of E. globulus extracted with acetone, ethanol and petroleum ether was studied using well diffusion method against the selected bacterial and fungal isolates. The antimicrobial susceptibility of the bacteria and fungi to various concentrations of the extract was evaluated by the micro broth dilution method. The preliminary phytochemical screening was performed in the acetone extract of E. globulus. The presence of functional groups in the acetone extract of the leaves of E. globulus was determined by Fourier to transform infrared (FT-IR) spectroscopy.Results: Results suggested that the acetone extract exhibited maximum antimicrobial activity against the tested bacteria and fungi when compared with ciprofloxacin and fluconazole respectively (standard antibiotics). Minimum inhibitory concentration (MIC) showed that the wells containing a concentration of 12.5 mg/ml and 6.25 mg/ml of acetone extract inhibited the bacteria and fungi effectively. Phytochemical screening of acetone extract of E. globulus showed the presence of alkaloids, flavonoids, phenols, tannins, quinones, glycosides, steroids, terpenoids and leucoanthocyanides. FT-IR spectral analysis of the leaf extract of E. globulus showed the presence of functional groups such as–OH,-NH, C=O and–COOH.Conclusion: Thus to conclude, different plants and their formulations have been used in ayurveda to cure diseases for several decades. The findings of the study indicate the potential to exploit the leaves of Eucalyptus globulus in the treatment of microbial diseases.

scholarly journals Phytochemical Analysis and Antifungal Activity of Ficus Sycomorus L. (Moraceae) Extracts on some Dermatophytic Fungi

2021 ◽  
Vol 6 (1) ◽  
pp. 11-17
Author(s):  
Umar Lawal ◽  
◽  
L. Mansir ◽  
M. G. Sani
Keyword(s):  
Antifungal Activity ◽  
Methanol Extract ◽  
Fungal Infections ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Microsporum Canis ◽  
Phytochemical Screening ◽  
Ms Analysis ◽  
Dermatophytic Fungi ◽  
Ficus Sycomorus

Ficus sycomorus L is a large, semi-deciduous spreading savannah tree that belongs to the family (Moraceae) is used by many tribes in the treatments of skin infections. The aim of this study was to carry out phytochemical analysis and antifungal evaluation of F. sycomorus L. stem and leaf Methanol extract on some dermatophytic fungi. The stem and leaf crude extracts were obtained after drying using maceration and percolation with methanol as the solvent. The extract was subjected to qualitative phytochemical screening as well as GC-MS analysis for the presence of bioactive constituents. The antifungal inhibitory effect of the crude stem and leaf extracts was assessed by agar well diffusion methods. The phytochemical screening revealed that the leaves and stem-bark contained secondary metabolites such as tannins, flavonoids, alkaloids, saponins, reducing sugar and steroid. The GC-MS assay showed that the most prevailing compound in the methanolic stem-bark extract was 2, 9, Diazabicyclo (4, 4, 0) decane-3,10 dione, while in the methanolic leaves extract was acetic acid. The anti-dermatophytic assay of the extracts against Microsporum canis and Tricophyton tonsurans showed that the extracts inhibited the fungi with zones of inhibitions ranging from 6.33±0.47 to 24.33±0.47mm with the leaf extract exhibiting higher inhibition zones than the stem extract (p˂0.01). The study shows that both the stem and leaf methanol extract of Ficus sycomorus L possesses antifungal activity and should be considered as a potential antifungal agent in search of newer agents to address dermatophytic fungal infections Key words: Ficus sycomorus L, Phytochemical/GC-MS Analysis, Antifungal activity

scholarly journals Antimicrobial activities and phytochemical screening of leaf extract of Mikania micrantha H.B.K

2018 ◽  
Vol 30 ◽  
pp. 274-286
Author(s):  
Anjana Devkota ◽  
Anita Sahu
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Mikania Micrantha ◽  
Crude Leaf Extract

Antimicrobial activities and phytochemical screening of leaves of Mikania micrantha was tested in laboratory against phytopathogenic fungi and human pathogenic bacteria. The leaves samples were extracted in distilled water and methanol. The crude extracts of leaves were assessed in-vitro for antimicrobial activity using different concentrations (50, 100, 150,200, and 250 mg/ml) against five fungal strains (viz. Sclerotium rolfsii, Phytopthora capsici, Alternaria brassicae, Fusarium oxysporum and Botrytis cinerea) and six bacterial strains (Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, Bacillus subtilis, Enterococcusfaecalis and Staphylococcus aureus). Antifungal activity was tested by Poison Food Technique and Linear Mycelium growth Reduction (LMGR) percentage was determined. In crude extractof M. micrantha, the highest LMGR percentage was found in F. oxysporum in both distilledwater and methanol extract. Antibacterial activity was carried out by Disc Diffusion method. In the crude leaf extract of M. micrantha Gram negative bacteria was found more resistant than Gram positive bacteria. Methanol extract was found more effective in determining the Zone of Inhibition for all the strains of bacteria in all the concentrations. The plant extracts were found more effective in showing antibacterial activity than antifungal activity. The phytochemicalscreening revealed that the selected species contained tannin, saponins, alkaloid, flavonoid, cardiac glycosides and terpenoids. This result supports the potential of this plant species used as a new chemotherapeutic drug.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF WHOLE AERIAL PART OF THE HERB LEUCAS PLUKENETII SPRENG (FAMILY-LAMINACEAE)

2017 ◽  
Vol 9 (3) ◽  
pp. 87
Author(s):  
Uttam Borah ◽  
Biswajit Dash ◽  
Suvakanta Dash ◽  
Lawrence Kalita
Keyword(s):  
Antimicrobial Activity ◽  
Aerial Part ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Standard Methods ◽  
Aerial Parts ◽  
Phytochemical Constituents

Objective: To evaluate the antimicrobial activity of ethanolic extract of Leucas Plukenetii Spreng (family-laminaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of whole aerial parts of the herb Leucas Plukenetiispreng (family-laminaceae) was prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungal strains using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the plant Leucas Plukenetii Spreng. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. The antimicrobial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of whole aerial parts of the Leucas Plukenetii Spreng contains the high presence of phytochemicals. The ethnaolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals Determination of Phytoconstituents and Antimicrobial activities of aqueous and methanol extracts of Neem (Azadirachta indica) leaves

2021 ◽  
pp. 60-67
Author(s):  
Odangowei Inetiminebi Ogidi ◽  
Mike NoahAyebabogha ◽  
Patricia Ukamaka Eze ◽  
Omu Okiemute ◽  
Chinaza Esther Okafor
Keyword(s):  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Plant Resources ◽  
Methanol Extracts ◽  
Neem Leaves

Neem (Azadirachta Indica)is a plant that has been used as traditional medicine for a household remedy against various human ailments since ancient times. The objective of this study was to determine the phytoconstituents and antimicrobial activities of aqueous and methanol extracts of A. indica leaves. Qualitative and quantitative phytochemical analysis was carried out using the standard method of Association of Official Analytical Chemist and the antimicrobial activity of the concentrated extracts was evaluated by determination of the diameter of zone of inhibition against the microorganisms using the agar well diffusion method. Phytochemical screening of the leaves gave a positive result for saponins, tannins, steroids, glycoside, terpenoids, flavonoids, and alkaloids. The antimicrobial results show that both plant extracts had antimicrobial activity against the test organisms, the aqueous extracts were found to show the greater antimicrobial effect on Shigellasp (10.0±1.0 mm), Staphylococcus sp (10.0±1.0 mm), Penicilliumsp (9.2±0.8 mm) and Mould (10.2±1.3 mm) while methanol extract shows maximum efficacy on E. coli (26±1.0 mm), Vibrio sp (10.6±0.6 mm) and Yeast (9.2±0.3 mm). Therefore, these findings support the traditional knowledge of local users and it is a preliminary, scientific, validation for the use of neem leaves for the antimicrobial activity to promote proper conservation and sustainable use of the plant resources.

scholarly journals PHYTOCHEMICAL SCREENING OF VOLVARIELLA VOLVACEA (STRAW MUSHROOM) EXTRACT FROM ACEH’S LOCAL CULTIVATION

2018 ◽  
Vol 18 (1) ◽  
pp. 32-37
Author(s):  
Sadli .
Keyword(s):  
Secondary Metabolite ◽  
Methanol Extract ◽  
Phytochemical Analysis ◽  
Volvariella Volvacea ◽  
Phytochemical Screening ◽  
Phytochemical Content ◽  
Mushroom Extract ◽  
Straw Mushroom

Abstract. A research on analyzing the phytochemical content in Volvariella volvacea (straw mushroom) extract from a local cultivation in Aceh has been conducted. Straw mushroom is potentially a medicine ingredient. This study aims to examine the secondary metabolite compounds in the methanol extract of straw mushroom. The extraction of the secondary metabolite compounds was undertaken by using the maceration method with methanol solvent. Phytochemical test was conducted qualitatively by using the meyer’s reagent, bouchardat, dragendrof, FeCl3, HCl 0.1 M, HCl 2N and Lieberman – bouchardat. Phytochemical analysis was carried out on flavonoids, alkaloids, glycosides, saponins, tannins and terpenoids. The phytochemical analysis’ result shows that the straw mushroom extract contains alkaloids, flavonoids, terpenoids, and saponins.Keywords: straw mushroom, methanol extract, maceration method, phytochemical screening 

scholarly journals Volatiles from Selected Apiaceae Species Cultivated in Poland—Antimicrobial Activities

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 695
Author(s):  
Jaroslaw Widelski ◽  
Konstantia Graikou ◽  
Christos Ganos ◽  
Krystyna Skalicka-Wozniak ◽  
Ioanna Chinou
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Gram Positive ◽  
Ongoing Research ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Bacteria And Fungi

As part of our ongoing research on phytoconstituents that can act as promising antimicrobial agents, the essential oils of nine selected Apiaceae plants, cultivated in Poland, were studied. The volatiles of the aerial parts with fruits (herba cum fructi) of Silaum silaus, Seseli devenyense, Seseli libanotis,Ferula assa-foetida, Glehnia littoralis and Heracleum dulce, in addition to the fruits (fructi) of Torilis japonica and Orlaya grandiflora as well as of the aerial parts (herba) of Peucedanum luxurians were investigated through Gas Chromatography–Mass Spectrometry to identify more than 60 different metabolites. The essential oils from S. devenyense, H. dulce, T. japonica and P. luxurians are reported for the first time. All examined species were also assayed for their antimicrobial activities against several human pathogenic Gram-positive and -negative bacteria and fungi. The species H. dulce, S. devenyense and S. libanotis exerted the strongest antimicrobial activity, mostly against Gram-positive bacteria strains (MIC values 0.90–1.20 mg/mL). To the best of our knowledge, this is the first attempt to determine the antimicrobial activity of the above Apiaceae species.

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