Anti-HIV-1 Activities and Chemical Constituents from Leaves and Twigs of Santisukia pagetii (Bignoniaceae)

The first phytochemical investigation of the leaves and twigs of Santisukia pagetii (Bignoniaceae) using a bioassay-guided fractionation led to the isolation and identification of seventeen known compounds, including four triterpenoids, 3- O-acetylpomolic acid (1), ursolic acid (2), 3- O-acetylursolic acid (3) and siaresinolic acid (4), three iridoid glycosides, specioside (5), verminoside (6) and ambiguuside (7), three flavonoid glycosides, luteolin-7- O-neohesperidoside (8), apigenin-7- O-neohesperidoside (9) and isoquercitrin (10), two phenolic compounds, p-coumaric acid (11) and caffeic acid (12), one monoterpenoid, ( 6S)-menthiafolic acid (13), together with α-D-glucose (14), β-D-maltose (15), β-sitosterol 3- O-β-D-glucopyranoside (16), a mixture of β-sitosterol (17A) and stigmasterol (17B). Compounds 1–13 were isolated from Santisukia genus for the first time. In addition, compounds 1–7, 9 and 10 were found to be active against HIV-1 in anti-syncytium assay, while only compounds 1 and 3 were found to be active (84.4% and 87.2% inhibition at 200 μg/mL, with IC50 values of 290.96 and 210.34 μM, respectively) against HIV-1 reverse transcriptase. Moreover, anti-HIV-1 activities of compounds 1, 3–8, and 13 were reported for the first time.

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Chemical Constituents of Cichorium intybus and their Inhibitory Effects against Urease and α-Chymotrypsin Enzymes

Natural Product Communications ◽

10.1177/1934578x1100600817 ◽

2011 ◽

Vol 6 (8) ◽

pp. 1934578X1100600

Author(s):

Sumayya Saied ◽

Shazia Shah ◽

Zulfiqar Ali ◽

Ajmal Khan ◽

Bishnu P. Marasini ◽

...

Keyword(s):

Chemical Constituents ◽

2D Nmr ◽

Cichorium Intybus ◽

Spectroscopic Techniques ◽

Inhibitory Effects ◽

Isolation And Identification ◽

Aerial Parts ◽

Phytochemical Investigation ◽

Hydroxyphenylacetic Acid ◽

First Time

Phytochemical investigation of the aerial parts of Cichorium intybus L. resulted in the isolation and identification of two new natural metabolites, 2,6-di[but-3( E)-en-2-onyl]naphthalene (1), and 3,3′,4,4′-tetrahydroxychalcone (2), along with nine known compounds. Their structures were determined by spectroscopic techniques including 1D and 2D NMR. The known compounds were identified as scopoletin (3), 4-hydroxyphenylacetic acid (4), 3-hydroxy-4-methoxybenzoic acid (5), 4,4′-dihydroxychalcone (6), 6,7-dihydroxycoumarine (7), 1-triacontanol (8), lupeol (9), β-sitosterol (10), and β-sitosterol-3- O-β-glucopyranoside (11). Compounds 4-6 and 8 are reported for the first time from C. intybus. Compounds 2 and 3 showed weak inhibitory activities against urease and α-chymotrypsin enzymes, respectively.

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Chemical Constituents of Adesmia Boronioides and Evaluation of their Antioxidant Activity

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2015.8.3.5 ◽

2015 ◽

Vol 8 (3) ◽

pp. 2919-2223

Author(s):

Orlando Muñoz ◽

C Delporte

Keyword(s):

Chemical Constituents ◽

Antioxidant Properties ◽

2D Nmr ◽

Isolation And Identification ◽

Ongoing Research ◽

Phytochemical Investigation ◽

Kaempferol Glycoside ◽

Pharmacologically Active ◽

Pharmacologically Active Compounds ◽

First Time

As part of our ongoing research into the pharmacologically active compounds of Chilean flora, here we did phytochemical investigation of Adesmia boronioides Hook.f (fabaceae). (Papilonaceae) leaves. This effort has led to isolation and identification of liqueritigenin, isoliquiritigenin, sesamin, isodrimenin and a kaempferol glycoside, kaempferol 3-O-β-D-[galactopyranosyl (1–2) rhamnoside]-7-O –α-L-rhamnopyranoside.Compound structures were identified by 1D- and 2D-NMR experiments, mass and UV spectrometry and comparison with data from the literature. This is the first time these compounds have been isolated from this plant’s leaves.These active constituents have been studied further pharmacologically. Antioxidant properties in A. boronioides leaves were determined.

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Anti-HIV Activity of Alkaloids from Dasymaschalon echinatum

Natural Product Communications ◽

10.1177/1934578x1801300110 ◽

2018 ◽

Vol 13 (1) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Samreang Bunteang ◽

Waraporn Chanakul ◽

Sakchai Hongthong ◽

Chutima Kuhakarn ◽

Watcharra Chintakovid ◽

...

Keyword(s):

Reverse Transcriptase ◽

Inhibitory Activity ◽

First Report ◽

Aerial Parts ◽

Amide Derivatives ◽

Bioassay Guided Fractionation ◽

First Time ◽

Anti Hiv ◽

Hiv 1

Bioassay-guided fractionation of the aerial parts of Dasymaschalon echinatum led to the isolation of five known aristolactams; aristolactam AII (1), aristolactam BII (2), piperolactam A (3), piperolactam C (4), and goniopedaline (5), together with two aphorphine alkaloids; duguevalline (6) and noraristolodione (7) and two amide derivatives; asperphenamate (8), and N -benzoyl-L-phenylalaninol (9). Alkaloids 2 and 7 were isolated for the first time from the Dasymaschalon genus. The anti-HIV 1 reverse transcriptase (RT) activity of all isolated compounds was determined. Except for aristolactam BII (2), this is the first report of the anti-HIV 1-RT activity of compounds 1 and 3-9. Compounds 1, 3, 5, 6 and 8 showed weak anti-HIV 1-RT inhibitory activity with IC50 ranging from 112.74 to 225.55μM.

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Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

Molecules ◽

10.3390/molecules26061694 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1694

Author(s):

Kamel Arraki ◽

Perle Totoson ◽

Alain Decendit ◽

Andy Zedet ◽

Justine Maroilley ◽

...

Keyword(s):

Inhibitory Activity ◽

Methanolic Extract ◽

Significant Inhibition ◽

Isolation And Identification ◽

Vasorelaxant Effect ◽

Methanolic Extracts ◽

Phytochemical Investigation ◽

Ic50 Values ◽

First Time

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1–2), together with three other stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The arginase inhibitory activity of compounds 1–7 was evaluated through an in vitro arginase inhibitory assay using purified liver bovine arginase. As a result, five compounds (1, 4–7) showed significant inhibition of arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural arginase inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.

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CONTRIBUTE TO STUDY ON CHEMICAL CONSTITUENTS OF URENA LOBATA (L.) OF VIETNAM

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/57/2/13019 ◽

2019 ◽

Vol 57 (2) ◽

pp. 162

Author(s):

Quan Minh Pham ◽

Hoai Van Thi Tran ◽

Lam Tien Do ◽

Phuong Lan Doan ◽

Inh Thi Cam ◽

...

Keyword(s):

Mental Disorders ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Constituents ◽

2D Nmr ◽

Spectroscopic Methods ◽

Tree Roots ◽

Chemical Structures ◽

Phytochemical Investigation ◽

First Time

Urena lobata L. is used in Vietnamese traditional medicine for the treatment of several diseases. Tree roots are used to treat rheumatism, dysentery, poor digestion, flu, tonsils, malaria, asthma, goiter. Flowers are used to treat chickenpox, fever, and mental disorders. Branches, leaves or whole trees used to treat injuries bruises, rheumatism, mastitis, bites. Phytochemical investigation of the n-hexan and ethyl acetate extract of Urena lobata L. led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-D-glucopyranoside (2), a-acetylamino-phenylpropyl a-benzoylamino-phenylpropanoate (3), quercetin (4), and trans-tiliroside (5). Their chemical structures were determined by spectroscopic methods including MS, 1D, 2D NMR and comparing with those reported in previous papers. Two compounds 3, 5 were isolated for the first time from Urena lobata plant.

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A New Oxoaporphine Alkaloid from the Root of Dasymaschalon glaucum

Natural Product Communications ◽

10.1177/1934578x1801301115 ◽

2018 ◽

Vol 13 (11) ◽

pp. 1934578X1801301

Author(s):

Weerachai Silprakob ◽

Nuntaporn Sukhamsri ◽

Chutima Kuhakarn ◽

Sakchai Hongthong ◽

Surawat Jariyawat ◽

...

Keyword(s):

Spectroscopic Data ◽

Chemical Structures ◽

Phytochemical Investigation ◽

Anti Hiv ◽

Hiv 1

The first phytochemical investigation of the root of Dasymaschalon glaucum led to the isolation of a new oxoaporphine alkaloid 1 together with seven known compounds, duguevalline (2), stigmalactam (3), enterocarpam III (4), desmosdumotin B (5), spiraeamide (6), kaempferol-3- O-glucopyranoside (7) and β-sitosterol (8). The chemical structures of all isolated compounds were established on the basis of their spectroscopic data. The cytotoxic and anti-HIV 1-RT activities of isolated compounds were also evaluated.

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In-Vitro Virucidal and Virustatic anti HIV-1 Effects of Extracts from Persea Americana Mill, (Avocado) Leaves

Antiviral Chemistry and Chemotherapy ◽

10.1177/095632029600700401 ◽

1996 ◽

Vol 7 (4) ◽

pp. 179-183 ◽

Cited By ~ 12

Author(s):

M.D. Wigg ◽

A.A. Al-Jabri ◽

S.S. Costa ◽

E. Race ◽

B. Bodo ◽

...

Keyword(s):

Syncytium Formation ◽

Persea Americana ◽

Reverse Phase ◽

Methanolic Extracts ◽

P24 Antigen ◽

Virucidal Effect ◽

First Time ◽

Anti Hiv ◽

Hiv 1

Aqueous (PA1) and methanolic extracts (PA2a–d; PA3) from the tropical tree Persea americana Mill. (Lauraceae), were evaluated for their cellular toxicity and anti-HIV-1 activity both in virustatic and virucidal assays. With the exception of PA3 and PA2d, all extracts showed anti-HIV-1 activity at concentrations which were not toxic for the H9 indicator cells. From the methanol insoluble extract (PA2) four different fractions (PA2a–d) were obtained using reverse-phase column chromatography, and two of the fractions (b and c) showed detectable virucidal effect. One fraction (PA2a) showed virustatic effects inhibiting HIV syncytium formation and viral p24 antigen formation at concentrations which were not toxic for the indicator cells. The results demonstrate for the first time that extracts from P. americana leaves have moderate anti-HIV-1 activity in vitro.

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STUDY ON CHEMICAL CONSTITUENTS AND CYTOTOXICITY OF ASPIDISTRA LETREAE

HUE UNIVERSITY JOURNAL OF SCIENCE NATURAL SCIENCE ◽

10.26459/hueuni-jns.v129i2a.5656 ◽

2020 ◽

Vol 129 (1B) ◽

Author(s):

Duc Viet Ho

Keyword(s):

Methanol Extract ◽

Human Cancer ◽

Chemical Constituents ◽

Hep G2 ◽

Pure Compound ◽

Human Cancer Cell Lines ◽

Phytochemical Investigation ◽

Reported Data ◽

First Time

A phytochemical investigation of whole Aspidistra letreae plants led to the isolation of 2H-chromen-2-one (1), α-tocopherol (2), (E)-phytol (3), asparenydiol (4) and (25S)-spirost-1β,3α,5β-triol (5). Their structures were determined on the basis of NMR spectral evidences and in comparison with the reported data. Of these, asparenydiol (4) was isolated from the genus Aspidistra for the first time. This is also the first report on the separation and structural determination of (25S)-spirost-1β,3α,5β-triol (5) as a pure compound. The methanol extract exhibited a moderate cytotoxicity against the LU-1, HeLa, MDA-MB-231, Hep-G2, and MKN-7 human cancer cell lines with IC50 values ranging from 52.58 ± 3.65 to 64.78 ± 4.89 μg/mL.

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Chemical constituents and cytotoxicity of Aspidistra letreae

HUE UNIVERSITY JOURNAL OF SCIENCE NATURAL SCIENCE ◽

10.26459/hueuni-jns.v129i1b.5656 ◽

2020 ◽

Vol 129 (1B) ◽

pp. 31-39

Author(s):

Duc Viet Ho ◽

Hanh Nhu Thi Hoang ◽

Khue Minh Vo ◽

Anh Tuan Le ◽

Hoai Thi Nguyen

Keyword(s):

Human Cancer ◽

Chemical Constituents ◽

Hep G2 ◽

Human Cancer Cell Lines ◽

Phytochemical Investigation ◽

Ic50 Values ◽

Reported Data ◽

Whole Plants ◽

First Time

A phytochemical investigation of whole Aspidistra letreae plants led to the isolation of 2H-chromen-2-one (1), α-tocopherol (2), (E)-phytol (3), asparenydiol (4) and (25S)-spirost-1β,3α,5β-triol (5). Their structures were determined on the basis of NMR spectral evidences and in comparison with the reported data. Of these, asparenydiol (4) was isolated from the genus Aspidistra for the first time. This is also the first report on the separation and structural determination of (25S)-spirost-1β,3α,5β-triol (5) as a pure compound. The methanol extract from the whole plants of Aspidistra letreae exhibits moderate cytotoxicity against the LU-1, HeLa, MDA-MB-231, Hep-G2, and MKN-7 human cancer cell lines with IC50 values ranging from 52.58 ± 3.65 to 64.78 ± 4.89 μg/mL.

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7α-Hydroxyfriedelan-3-one-26-ol-29-oic acid and other Constituents from Pileostegia viburnoides var. glabrescens

Natural Product Communications ◽

10.1177/1934578x1601100716 ◽

2016 ◽

Vol 11 (7) ◽

pp. 1934578X1601100

Author(s):

Sheng-Huang Chen ◽

Juan Yu ◽

Qin-Wen Li ◽

Jian-Ping Zhao ◽

David E. Wedge ◽

...

Keyword(s):

Leukemia Cell ◽

Agrostis Stolonifera ◽

Leukemia Cell Line ◽

X Ray Diffraction ◽

Isolation And Identification ◽

X Ray ◽

Phytochemical Investigation ◽

Raw 264.7 Macrophage Cells ◽

Murine Leukemia Cell ◽

First Time

Phytochemical investigation of the roots and rhizomes of Pileostegia viburnoides var. glabrescens led to the isolation and identification of 31 compounds (1-31), 25 of which (1-2, 4-7, 9-12, 15-18, 21-31) were isolated from the Pileostegia genus for the first time. 7α-Hydroxyfriedelan-3-one-26-ol-29-oic acid (1) is a new friedelane-type triterpene. The structure of n-butyl-β-D-fructopyranoside (2) was determined by single-crystal X-ray diffraction. Compounds 4 and 12 displayed marginal cytotoxicity against the P388 murine leukemia cell line with IC50 values of 13.4 μM and 25.0 μM, respectively. Compound 23 exhibited marginal anti-inflammatory activity by the inhibition of lipopolysaccharide induced nitric oxide production in RAW 264.7 macrophage cells, with an IC50 value of 32.0 μM. Compounds 3, 8-10, 25, and 27 were phytotoxic to the dicot Lactuca sativa (lettuce) and/or the monocot Agrostis stolonifera (bentgrass).

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