scholarly journals Pharmacological Assessment of the Carvacrol Chemotype Essential Oil From Plectranthus amboinicus Growing in Cuba

2020 ◽  
Vol 15 (10) ◽  
pp. 1934578X2096223
Author(s):  
Lianet Monzote ◽  
Alexander M. Scherbakov ◽  
Ramón Scull ◽  
Yamilet I. Gutiérrez ◽  
Prabodh Satyal ◽  
...  
Keyword(s):  
Essential Oil ◽  
Trypanosoma Brucei ◽  
Inhibitory Concentration ◽  
Human Tumor ◽  
Chemical Characterization ◽  
Nutritional Properties ◽  
Main Compound ◽  
Mass Spectrometric Detector ◽  
Plectranthus Amboinicus ◽  
Mcf 7

Plant-derived products are employed in various public health practices and have been considered as a major source of medicines. The genus Plectranthus (Lamiaceae) has been widely respected for its nutritional properties, its essential oil, and its therapeutic values. In the present work, the chemical characterization, antimicrobial, antiparasitic, and cytotoxic properties of the essential oil from Plectranthus amboinicus (Lour.) Spreng and its main compound carvacrol were studied. Twenty-one components were identified in the oil by gas chromatography coupled with a mass spectrometric detector. In this oil, carvacrol constitutes the major compound (71%), which represented the more abundant chemotype. The essential oil did not inhibit growth of Escherichia coli, Staphylococcus aureus, Candida albicans, Trypanosoma cruzi, or Leishmania infantum, but displayed activity against Plasmodium falciparum (half-maximal inhibitory concentration [IC50] = 5.9 µg/mL), Trypanosoma brucei (IC50 = 34.9 µg/mL), and Leishmania amazonensis (IC50 = 58.2 µg/mL), and the human tumor-derived cell lines MCF-7 (IC50 = 29.1 µg/mL), MDA-MB-231 (IC50 = 41.5 µg/mL), and 22Rv1 (IC50 = 29.6 µg/mL), but no cytotoxicity was observed against nonmalignant macrophages. The antiproliferative activity of the oil could be attributed to carvacrol. However, this compound showed certain level of cytotoxicity, which suggests unspecific activity. This study provides evidence about antimicrobial and anticancer potential of the essential oil from P. amboinicus against protozoa and neoplastic diseases, particularly as an antimalarial natural product.

scholarly journals Action ofCoriandrum sativumL. Essential Oil upon OralCandida albicansBiofilm Formation

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
V. F. Furletti ◽  
I. P. Teixeira ◽  
G. Obando-Pereda ◽  
R. C. Mardegan ◽  
A. Sartoratto ◽  
...  
Keyword(s):  
Essential Oil ◽  
Biofilm Formation ◽  
Plant Material ◽  
Inhibitory Concentration ◽  
Chemical Characterization ◽  
Synergistic Action ◽  
Biofilm Growth ◽  
Water Distillation ◽  
Microdilution Method

The efficacy of extracts and essential oils fromAllium tuberosum, Coriandrum sativum, Cymbopogon martini, Cymbopogon winterianus,andSantolina chamaecyparissuswas evaluated againstCandidaspp. isolates from the oral cavity of patients with periodontal disease. The most active oil was fractionated and tested againstC. albicansbiofilm formation. The oils were obtained by water-distillation and the extracts were prepared with macerated dried plant material. The Minimal Inhibitory Concentration—MIC was determined by the microdilution method. Chemical characterization of oil constituents was performed using Gas Chromatography and Mass Spectrometry (GC-MS). C. sativum activity oil upon cell and biofilm morphology was evaluated by Scanning Electron Microscopy (SEM). The best activities against planktonicCandidaspp. were observed for the essential oil and the grouped F8–10fractions fromC. sativum. The crude oil also affected the biofilm formation inC. albicanscausing a decrease in the biofilm growth. Chemical analysis of the F8–10fractions detected as major active compounds, 2-hexen-1-ol, 3-hexen-1-ol and cyclodecane. Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents. The activity ofC. sativumoil demonstrates its potential for a new natural antifungal formulation.

scholarly journals Antimildew Effects of Plectranthus amboinicus Leaf Essential Oil on Paper

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986290 ◽  
Author(s):  
Kuang-Ping Hsu ◽  
Chen-Lung Ho
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Trichoderma Viride ◽  
Gas Chromatography Mass Spectrometry ◽  
Aspergillus Clavatus ◽  
Flame Ionization ◽  
Leaf Oil ◽  
Leaf Essential Oil ◽  
Plectranthus Amboinicus

In this study, we evaluated the antimildew effects on paper of Plectranthus amboinicus (Lour.) Spreng leaf essential oil prepared through hydrodistillation. The oil was isolated with a Clevenger-type apparatus and then characterized through gas chromatography with flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of 43 compounds were identified, representing 99.5% of the oil. The main ingredients were carvacrol (50.0%), γ-terpinene (13.1%), and β-caryophyllene (11.3%). The leaf oil had excellent antimildew effects; its minimum inhibitory concentration (MIC) values against Aspergillus clavatus, A. niger, Cladosporium cladosporioides, Chaetomium globosum, Myrothecium verrucaria, Penicillium citrinum, and Trichoderma viride were 100, 200, 75, 75, 100, 150, and 150 μg/cm2 respectively. The leaf oil was further partitioned into 6 derivative fractions (PO1-PO6). PO2 had excellent antimildew effects on the abovementioned strains; at a concentration of 100 μg/cm2, all were 100% inhibited, except A. niger, which was 75.8% inhibited. Carvacrol, the main ingredient of PO2, was then isolated and used for antimildew tests; carvacrol had excellent antimildew activities. Since both P. amboinicus leaf essential oil and carvacrol were proved to have excellent antimildew effects on paper, they are worth further research and development.

scholarly journals Essential oil composition and cytotoxic activity in two species of the plant genus Vismia (Hypericaceae) from the Venezuelan Andes

2020 ◽  
Vol 68 (3) ◽  
Author(s):  
Janne Rojas Vera ◽  
Alexis Buitrago Díaz ◽  
Francisco A. Arvelo ◽  
Felipe J. Sojo ◽  
Alírica I. Suarez ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Tumor Cells ◽  
Biological Activities ◽  
Human Tumor ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Mcf 7

Abstract. Introduction: The Vismia genus belongs to the Hypericaceae family and comprises around 57 species of which 17 have been located in Venezuela. Previous investigations have been carried out in extracts as well as pure isolated compounds, revealing antimicrobial, antioxidant and anti-HIV, among other, biological activities. Objective: This investigation aims to determine the cytotoxic activity of essential oils from leaves of Vismia baccifera Triana & Planch (VBJ and VBV) and Vismia macrophylla Kunth (VM) collected in three different locations of the Venezuelan Andean region. Methods: Essential oils obtained by hydrodistillation were analyzed using gas chromatography-mass spectrometry (GC-MS) and their cytotoxic activity was analyzed following the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Human tumor cell lines from SKBr3, MCF-7 and PANC-1, two breast carcinomas and one pancreatic adenocarcinoma of ductal type, were tested with the oil samples and human dermis fibroblasts were used as non-tumor cells. Results: β-caryophyllene and trans-caryophyllene were present as major components in VBJ and VBV, respectively, while γ-bisabolene was the main component in the VM sample. Anticancer activity was observed on V. baccifera essential oil against SKBr3, MCF-7 and PANC-1. The selectivity index showed that VBV is highly selective against the SKBr3 cell line and has no activity against non-tumor cells. Conclusions: These results are considered a contribution to natural products research and may provide supportive data for future studies on cancer.

scholarly journals Antibiotic Potential and Chemical Composition of the Essential Oil of Piper caldense C. DC. (Piperaceae)

2020 ◽  
Vol 10 (2) ◽  
pp. 631 ◽  
Author(s):  
José Weverton Almeida Bezerra ◽  
Felicidade Caroline Rodrigues ◽  
Rafael Pereira da Cruz ◽  
Luiz Everson da Silva ◽  
Wanderlei do Amaral ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Chemical Characterization ◽  
Fungal Morphology ◽  
Caryophyllene Oxide ◽  
Minimum Fungicidal Concentration ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Low Activity

Infections by multiresistant microorganisms have led to a continuous investigation of substances acting as modifiers of this resistance. By following this approach, the chemical composition of the essential oil from Piper caldense leaf and its antimicrobial potential were investigated. The antimicrobial activity was determined by broth microdilution method providing values for minimum inhibitory concentration (MIC), IC50, and minimum fungicidal concentration (MFC). The essential oil was tested as a modulator for several antibiotics, and its effect on the morphology of Candida albicans (CA) strains was also investigated. The chemical characterization revealed an oil composed mainly of sesquiterpenes. Among them are caryophyllene oxide (13.9%), spathulenol (9.1%), δ-cadinene (7.6%) and bicyclogermacrene (6.7%) with the highest concentrations. The essential oil showed very low activity against the strains of CA with the lowest values for IC50 and MFC of 1790 μg/mL and 8192 μg/mL, respectively. The essential oil modulated the activity of fluconazole against CA URM 4387 strain, which was demonstrated by the lower IC50 obtained, 2.7 μg/mL, whereas fluconazole itself presented an IC50 of 7.76 μg/mL. No modulating effect was observed in the MFC bioassays. The effect on fungal morphology was observed for both CA INCQS 40006 and URM 4387 strains. The hyphae projection was completely inhibited at 4096 μg/mL and 2048 μg/mL, respectively. Thus, the oil has potential as an adjuvant in antimicrobial formulations.

Chemical Characterization of Lavandula dentata Essential Oil Cultivated in Chile and Its Antibiofilm Effect against Candida albicans

Planta Medica ◽  
2020 ◽  
Vol 86 (16) ◽  
pp. 1225-1234
Author(s):  
Andrea Müller-Sepúlveda ◽  
Camila Cid Chevecich ◽  
José A. Jara ◽  
Carolina Belmar ◽  
Pablo Sandoval ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Chemical Characterization ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Inhibitory Concentrations ◽  
Adhesion Ability ◽  
Viability Assay

Abstract Candida albicans is the most common human fungal pathogen, and with the increase in resistance rates worldwide, it is necessary to search for new pharmacological alternatives. Lavandula dentata L. essential oil is recognized as having antimicrobial properties. However, its effect against fungal biofilms has been poorly described. C. albicans-related infections involve the development of biofilms, which are highly resistant to conventional antifungals. In this work, we evaluated the antibiofilm effect of L. dentata L. essential oil against C. albicans. First, we characterized the essential oil by gas chromatography-mass spectrometry. The antifungal effect on C. albicans reference strains was evaluated by a disk diffusion assay and the minimal inhibitory concentration was obtained through a microdilution assay. The effect of the essential oil on the adhesion ability of C. albicans was determined through a crystal violet assay, and morphogenesis inhibition was assessed by light microscopy. The effect of the essential oil on the microarchitecture of biofilms was evaluated through scanning electron microscopy. Finally, the antibiofilm effect was evaluated through an adapted biofilm scratch assay and XTT viability assay. The main constituent of the essential oil was the monoterpenoid eucalyptol (60%). The essential oil presented minimal inhibitory concentrations of 156 and 130 µg/mL against two strains assayed. This minimal inhibitory concentration inhibited adhesion, morphogenesis, biofilm formation, altered microarchitecture, and decreased the viability of established biofilms formed on abiotic surfaces for both strains assayed. This study demonstrates that the essential oil from L. dentata could be a promising treatment against C. albicans biofilms.

scholarly journals Chemical Composition of Blumea lacera Essential Oil from Nepal. Biological Activities of the Essential Oil and (Z)-Lachnophyllum Ester

2015 ◽  
Vol 10 (10) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Prabodh Satyal ◽  
Bhuwan K. Chhetri ◽  
Noura S. Dosoky ◽  
Samon Shrestha ◽  
Ambika Poudel ◽  
...  
Keyword(s):  
Essential Oil ◽  
Biological Activities ◽  
Human Tumor ◽  
Gas Chromatography Mass Spectrometry ◽  
Gel Chromatography ◽  
Aerial Parts ◽  
Silica Gel Chromatography ◽  
Antibacterial And Antifungal ◽  
Blumea Lacera ◽  
Mcf 7

The essential oil from the aerial parts of Blumea lacera collected from Biratnagar, Nepal, has been obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major component from the oil, (Z)-lachnophyllum ester, was isolated by preparative silica gel chromatography. B. lacera oil was dominated by (Z)-lachnophyllum ester (25.5%), (Z)-lachnophyllic acid (17.0%), germacrene D (11.0%), (E)-β-farnesene (10.1%), bicyclogermacrene (5.2%), (E)-caryophyllene (4.8%), and (E)-nerolidol (4.2%). Also detected in the oil were (E)-lachnophyllic acid (3.3%) and (E)-lachnophyllum ester (1.7%). (Z)-Lachnophyllum ester exhibited cytotoxic activity against MDA-MD-231, MCF-7, and 5637 human tumor cells, as well as antibacterial and antifungal activity.

scholarly journals Study on chemical composition, anti-pathogenic micro-organisms activity and inhibition activity against human breast cancer cell (MCF 7) of Juniperus sibirica essential oil

2019 ◽  
pp. 41-48
Author(s):  
Javzmaa N ◽  
Altantsetseg Sh ◽  
Sarantuya J ◽  
Tuul Ts ◽  
V G Shiretorova
Keyword(s):  
Breast Cancer ◽  
Essential Oil ◽  
Essential Oils ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Chemical Characterization ◽  
Cytotoxic Activities ◽  
Food Preservatives ◽  
Micro Organisms ◽  
Mcf 7

In recent years, due to the growing interest in natural products, plant essential oils have come more into the focus of phytomedicine [1,2]. Essential oils, one of the secondary metabolites in plants, are mixture of volatile organic compounds. They are used as food preservatives, additives, and natural remedies for food, cosmetic, pharmaceutical and agriculture sectors [2,3].In this study, we have investigated chemical characterization of needle essential oil from wild growing Juniperus sibirica in Mongolia and its antibacterial, antifungal and cytotoxic activities. In the essential oil of Juniperus sibirica needle, 78 compounds were identified. Among them α-pinene (48.6%), limonene + phellandrene (9.6%), myrtenyl acetate (4.03%), myrcene (3.3%) and β-pinene (3.0%) were identified as major constituents. This oil owned inhibitory activity against Bacillus. subtilis, Staphylococcus. aureus and Aspergillus. niger with 21, 17 and 11 mm clear zones. In addition, the oil at 50 μg/mL inhibited the growth of MCF-7 cell by 35.3% and at 100 μg/mL by 44.5%, respectively.The essential oil of Mongolian Juniperus sabina would be used as a preservative material in cosmetic and food products, as a bioactive agent in anti-inflammatory and wound healing products because of the determined antibacterial activity. In further, a detailed study on its anti-cancer and activity against pathogenic micro-organisms are necessary due to the promising anti-proliferative activity in breast cancer cell (MCF 7). Ямаан арц (Juniperus sibirica)-ны эфирийн тосны химийн бүрэлдэхүүн, бичил биетний эсрэг, хөхний хорт хавдрын (MCF 7) эсийн өсөлтийг дарангуйлах идэвхийн судалгаа Хураангуй: Сүүлийн жилүүдэд эфирийн тосны эрэлт ихсэж, тэдгээрийн хими, биологийн судалгаа эрчимтэй хийгдэх боллоо [1,2]. Эфирийн тос нь үнэртэн, гоо сайхан, шүдний эмчилгээ, хөдөө аж ахуйн бүтээгдэхүүн, ариутгагч бэлдмэл, хүнсний хадгалалтыг уртасгах, амт үнэр оруулах нэмэлт болон байгалийн гаралтай эмийн түүхий эдээр өргөн ашиглагддаг дэгдэмхий нэгдлүүдийн хольцоос тогтох ургамлын хоёрдогч метаболитуудын нэг юм [2,3].Бид энэхүү судалгаагаар Монголын ямаан арцны шилмүүсний эфирийн тосны химийн бүрэлдэхүүн, бичил биетний эсрэг, хөхний хорт хавдрын MCF 7 эсийг дарангуйлах идэвхийг тодорхойллоо. Ямаан арцны шилмүүсний эфирийн тосонд 78 бүрэлдэхүүн бодис илэрснээс -пинен-48.6%, лимонен+фелландрен-9.6%, миртенил ацетат-4.0%, β-пинен-3.0%, мирцен-3.6%, ∆3-карен- 3.4% зонхилон агуулагдаж байв. Энэ тосны бичил биетний эсрэг идэвхийг агар диффузын аргаар тодорхойлоход B.subtilis, S.aureus, A.niger бичил биетнүүдийн ургалтыг 21, 17, 11мм хүрээ үүсгэн дарангуйлсан бол MTT аргаар MCF-7 хөхний хорт хавдрын эсийн өсөлтийг дарангуйлах идэвхийг тодорхойлоход 50 мкг/мл тундаа 37.3%, 100 мкг /мл тундаа 44.5% дарангуйлав.Монголын ямаан арцны эфирийн тосыг түүний бичил биетний эсрэг идэвхэд үндэслэн гоо сайхан, хүнсний бүтээгдэхүүний хадгалалтыг уртасгах, идээт үрэвсэл, яр шархны эсрэг бэлдмэлийн биоидэвхт түүхий эдээр ашиглаж болох юм. Мөн хөхний хорт хавдрын эсийн өсөлтийг идэвхтэйгээр дарангуйлж байгаа учир цаашид ямаан арцны эфирийн тосны бичил биетний болон хавдрын эсрэг идэвхийн судалгааг нарийвчлан хийх нь зүйтэй. Түлхүүр үг: Juniperus sibirica, α-пинен, миртенил ацетат, B.subtilis, эфирийн тос .

scholarly journals Biosynthesis and chemical characterization of an intracellular red pigment of Talaromyces islandicus T101

2020 ◽  
Author(s):  
Igor Vinícius Pimentel Rodrigues ◽  
Katia Regina Assunção Borges ◽  
Neurene da Cruz ◽  
Amanda Mara Teles ◽  
Marcos Antonio Custódio Neto da Silva ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Chemical Characterization ◽  
Cancer Cell Line ◽  
Synthetic Dyes ◽  
E Coli ◽  
Food Borne ◽  
Diphenyltetrazolium Bromide ◽  
Alternative Sources ◽  
Mcf 7

ABSTRACTThe interest in red colorants by the food industry has been increasing recently due to its wide application in many foods and beverages, and also to the carcinogenic and teratogenic effects of some synthetic dyes. Many ascomycetous fungi are able to synthesize and produce pigments, rendering them as alternative sources of natural dyes that are independent of environmental conditions. Talaromyces islandicus TI01 was isolated from a marine-influenced environment that has been suffering for decades from anthropogenic actions in its body of water. Broth microdilution technique was performed to analyze the antimicrobial activity. For analysis of the cytotoxic activity, the MTT [3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide] assay was conducted. The chemical analysis of the extract was performed by LC/MS (liquid chromatography coupled to mass spectrometry). The minimum bactericidal concentration (MBC) of T. islandicus’ intracellular red pigmented extract (IRPE) for E. coli ATCC 25922 and S. aureus ATCC 25923 was 1000 μg/ml. The minimum inhibitory concentration (MIC) for E. coli was 250 μg/mL and for S. aureus 500 μg/mL, respectively, whereas for C. tropicalis ATCC 1369 was 62.5 μg/mL. IC50 for breast cancer cell line (MCF-7) was 45.43 ± 1.657 μg / mL. The major compounds present in the extract were: Luteoskyrin (1) and N-GABA-PP-V (6-[(Z)-2-Carboxyvinyl]-N-GABA-PP-V) (2). The results show that IRPE from T. islandicus TI01 has a prominent antibacterial activity against E. coli and S. aureus, making this pigment interesting for development of new food colorants and/or conservative agents, since these bacteria are food-borne pathogens.

scholarly journals Chemical Composition, Leishmanicidal and Cytotoxic Activities of the Essential Oils fromMangifera indicaL. var. Rosa and Espada

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Eduardo H. S. Ramos ◽  
Marcílio M. Moraes ◽  
Laís L. de A. Nerys ◽  
Silene C. Nascimento ◽  
Gardênia C. G. Militão ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Mangifera Indica ◽  
Human Tumor ◽  
Therapeutic Agents ◽  
Cytotoxic Activities ◽  
Human Tumor Cells ◽  
Main Compound ◽  
Ht 29 ◽  
Brazilian Biodiversity

The essential oils fromMangifera indicavar. Rosa and Espada latex were obtained by hydrodistillation and analyzed using GC-FID and GC-MS. Twenty-seven components were identified. The main compound in the essential oil fromM. indicavar. Espada (EOMiE) was terpinolene (73.6%). The essential oil ofM. indicavar. Rosa (EOMiR) was characterized by high amounts ofβ-pinene (40.7%) and terpinolene (28.3%). In the test for leishmanicidal activity against promastigotes forms ofL. amazonensis, EOMiR and EOMiE showed IC50(72 h) of 39.1 and 23.0 μg/mL, respectively. In macrophages, EOMiR and EOMiE showed CC50of 142.84 and 158.65 μg/mL, respectively. However, both were more specific to the parasite than macrophages, with values of selectivity index of 6.91 for EOMiE and 3.66 for EOMiR. The essential oils were evaluated for their cytotoxicity against the human tumor cells HEp-2, HT-29, NCI-H292, and HL-60. The EOMiR and EOMiE were most effective against the HL-60, with IC50values of 12.3 and 3.6 μg/mL, respectively. The results demonstrated that the essential oils ofM. indicacan destroyL. amazonensisand inhibit tumor cell growth. These findings contribute to the knowledge of the Brazilian biodiversity as a source of potential therapeutic agents.

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