scholarly journals Effect of Administering Sodium Chlorate in Drinking Water on Salmonella Typhimurium Concentrations in Weaned and Finished Pigs

2003 ◽  
Vol 44 (Suppl 1) ◽  
pp. P63
Author(s):  
Robin C Anderson ◽  
Todd R Callaway ◽  
Kenneth J Genovese ◽  
Timothy J Anderson ◽  
Thomas S Edrington ◽  
...  
1981 ◽  
Vol 44 (12) ◽  
pp. 917-920 ◽  
Author(s):  
A. STERSKY ◽  
B. BLANCHFIELD ◽  
C. THACKER ◽  
H. PIVNICK

Day-old chicks (Gallus domesticus) were treated with cultured feces of adult chickens according to the Nurmi concept and were challenged 2 days later with Salmonella typhimurium. Treated chicks were less susceptible to infection than untreated chicks (16% vs. 79% infected). Those treated chicks that did become infected, contaminated their drinking water with fewer Salmonella than the untreated chicks (maximum of 104/ml vs. ⩾ 107/ml). Fecally contaminated water may be a major source for spreading Salmonella infection within a flock.


2013 ◽  
Vol 11 (4) ◽  
pp. 629-635 ◽  
Author(s):  
L. M. Schaefer ◽  
V. S. Brözel ◽  
S. N. Venter

Investigations were carried out to evaluate and quantify colonization of laboratory-scale drinking water biofilms by a chromosomally green fluorescent protein (gfp)-tagged strain of Salmonella Typhimurium. Gfp encodes the green fluorescent protein and thus allows in situ detection of undisturbed cells and is ideally suited for monitoring Salmonella in biofilms. The fate and persistence of non-typhoidal Salmonella in simulated drinking water biofilms was investigated. The ability of Salmonella to form biofilms in monoculture and the fate and persistence of Salmonella in a mixed aquatic biofilm was examined. In monoculture S. Typhimurium formed loosely structured biofilms. Salmonella colonized established multi-species drinking water biofilms within 24 hours, forming micro-colonies within the biofilm. S. Typhimurium was also released at high levels from the drinking water-associated biofilm into the water passing through the system. This indicated that Salmonella could enter into, survive and grow within, and be released from a drinking water biofilm. The ability of Salmonella to survive and persist in a drinking water biofilm, and be released at high levels into the flow for recolonization elsewhere, indicates the potential for a persistent health risk to consumers once a network becomes contaminated with this bacterium.


1981 ◽  
Vol 44 (12) ◽  
pp. 909-916 ◽  
Author(s):  
H. PIVNICK ◽  
B. BLANCHFIELD ◽  
J. -Y. D'AOUST

Chicks (Gallus domesticus) were treated per os with 24-h-old anaerobic cultures of feces from mature chickens 1 day after hatching, challenged with Salmonella typhimurium in the drinking water 2 days later, and sacrificed on day 11 or 12; then the lower third of the intestinal tract was examined for salmonellae. Cultures of feces inoculated directly into the crop or added to the drinking water, even after holding at −70 C for 21 days, protected chicks against infection by S. typhimurium. Cultures serially subcultured daily up to four times were protective, and dilution to 1:80 in drinking water containing 4 % skim milk powder did not decrease their protective effect. Treated chicks were about 1000-fold more resistant to infection by Salmonella than untreated chicks.


2001 ◽  
Vol 64 (2) ◽  
pp. 255-258 ◽  
Author(s):  
ROBIN C. ANDERSON ◽  
SANDRA A. BUCKLEY ◽  
TODD R. CALLAWAY ◽  
KENNETH J. GENOVESE ◽  
LEON F. KUBENA ◽  
...  

Salmonella cause economic losses to the swine industry due to disease and compromised food safety. Since the gut is a major reservoir for Salmonella, strategies are sought to reduce their concentration in pigs immediately before processing. Respiratory nitrate reductase activity possessed by Salmonella also catalyzes the intracellular reduction of chlorate (an analog of nitrate) to chlorite, which is lethal to the microbe. Since most gastrointestinal anaerobes lack respiratory nitrate reductase, we conducted a study to determine if chlorate may selectively kill Salmonella within the pig gut. Weaned pigs orally infected with 8 × 107 CFU of a novobiocin- and nalidixic acid–resistant strain of Salmonella Typhimurium were treated 8 and 16 h later via oral gavage (10 ml) with 0 or 100 mM sodium chlorate. Pigs were euthanized at 8-h intervals after receiving the last treatment. Samples collected by necropsy were cultured qualitatively and quantitatively for Salmonella and for most probable numbers of total culturable anaerobes. A significant (P < 0.05) chlorate treatment effect was observed on cecal concentrations of Salmonella, with the largest reductions occurring 16 h after receiving the last chlorate treatment. An observed treatment by time after treatment interaction suggests the chlorate effect was concentration dependent. Chlorate treatment may provide a means to reduce foodborne pathogens immediately before harvest.


1999 ◽  
Vol 1999 (1) ◽  
pp. 821-823
Author(s):  
Martha A. Wolf

ABSTRACT The Clark Fork and Flathead Basin Sub-Area consists of five counties in the state of Montana and the Flathead Nation. A section of pipeline running through the Flathead reservation was closed by the Confederated Salish and Kootenai Tribes, necessitating product removal from the pipeline in Missoula, Montana, shipment via train and truck over mountainous areas, and reinjection into the pipeline in Thompson Falls, Montana. The area also has hazardous material response issues demonstrating the need for an integrated hazmat and oil response plan. The Sub-Area Committee (sAC) consists of federal, state, tribal, local, and industry responders, planners and Natural Resource Trustees. One week to the day after the first sAC meeting, a train derailment occurred in the designated area. Four cars containing chlorine, one car containing potassium cresylate, and one car containing sodium chlorate began leaking—resulting in one death, over 350 injuries, evacuation of the town of Alberton, closure of Clark Fork for all recreational activities, detour of air traffic and closure of 63 miles of Interstate 90 for 17 days. Thirty minutes after the Alberton derailment, another derailment occurred near Doxon, Montana (also in the planning area) releasing 1,000 gallons of diesel fuel into the Clark Fork River. The plan consists of a core plan that contains the information vital to the response activities, and a volume that has all backup information that is not essential during the response. The plan is also in digitized format that runs off GIS maps. The maps contain all contacts, spill/release sources, available equipment, drinking water intakes, schools, nursing homes, hospitals, fish, wildlife and other sensitive environments. This plan is believed to be the tool that will insure smoother, more efficient responses in the future.


2003 ◽  
Vol 82 (9) ◽  
pp. 1403-1406 ◽  
Author(s):  
JA Byrd ◽  
RC Anderson ◽  
TR Callaway ◽  
RW Moore ◽  
KD Knape ◽  
...  

2006 ◽  
Vol 4 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Maggy N. B. Momba ◽  
Veronica K. Malakate ◽  
Jacques Theron

In order to study the prevalence of enteric pathogens capable of causing infection and disease in the rural communities of Nkonkobe, bacterial isolates were collected from several surface water and groundwater sources used by the community for their daily water needs. By making use of selective culture media and the 20E API kit, presumptive Escherichia coli, Salmonella spp. and Vibrio cholerae isolates were obtained and then analysed by polymerase chain reaction assays (PCR). The PCR successfully amplified from water samples a fragment of E. coli uidA gene that codes for β-D-glucuronidase which is a highly specific characteristic of enteropathogenic E. coli, enterotoxigenic E. coli and entero-invasive E. coli. The PCR also amplified the epsM gene from water samples containing toxigenic V. cholerae. Although E. coli was mostly detected in groundwater sources, toxigenic V. cholerae was detected in both surface and groundwater sources. There was a possibility of Salmonella typhimurium in Ngqele and Dyamala borehole water samples. The presence of these pathogenic bacteria in the above drinking water sources may pose a serious health risk to consumers.


Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2683
Author(s):  
Michailia Angelopoulou ◽  
Konstantina Tzialla ◽  
Angeliki Voulgari ◽  
Mary Dikeoulia ◽  
Ioannis Raptis ◽  
...  

Biosensors represent an attractive approach for fast bacteria detection. Here, we present an optical biosensor for the detection of Salmonella typhimurium lipopolysaccharide (LPS) and Salmonella bacteria in drinking water, based on white light reflectance spectroscopy. The sensor chip consisted of a Si die with a thin SiO2 layer on top that was transformed into a biosensor through the immobilization of Salmonella LPS. The optical setup included a reflection probe with seven 200 μm fibers, a visible and near-infrared light source, and a spectrometer. The six fibers at the reflection probe circumference were coupled with the light source and illuminated the biosensor chip vertically, whereas the central fiber collected the reflected light and guided it to the spectrometer. A competitive immunoassay configuration was adopted for the analysis. Accordingly, a mixture of LPS or bacteria solution, pre-incubated for 15 min, with an anti-Salmonella LPS antibody was pumped over the chip followed by biotinylated secondary antibody and streptavidin for signal enhancement. The binding of the free anti-Salmonella antibody to chip-immobilized LPS led to a shift of the reflectance spectrum that was inversely related to the analyte concentration (LPS or bacteria) in the calibrators or samples. The total assay duration was 15 min, and the detection limits achieved were 4 ng/mL for LPS and 320 CFU/mL for bacteria. Taking into account the low detection limits, the short analysis time, and the small size of the chip and instrumentation employed, the proposed immunosensor could find wide application for bacteria detection in drinking water.


1946 ◽  
Vol 25 (5) ◽  
pp. 476-478 ◽  
Author(s):  
H.C. Gauger ◽  
R.E. Greaves

2003 ◽  
Vol 66 (4) ◽  
pp. 660-663 ◽  
Author(s):  
YONG SOO JUNG ◽  
ROBIN C. ANDERSON ◽  
JAMES A. BYRD ◽  
THOMAS S. EDRINGTON ◽  
RANDLE W. MOORE ◽  
...  

The effects of two feed supplements on Salmonella Typhimurium in the ceca of market-age broilers were determined. Broilers orally challenged 6 days before slaughter with a novobiocin- and nalidixic acid–resistant strain of Salmonella Typhimurium were divided into one of four groups (20 birds each). The first group (the control group) received no treatment, the second group received sodium nitrate (SN) treatment (574 mg of NaNO3 per kg of feed), the third group received experimental chlorate product (ECP) treatment (15 mM NaClO3 equivalents), and the fourth group received ECP treatment in combination with SN treatment. The SN treatment was administered via feed for 5 days immediately before slaughter, and ECP was provided via ad libitum access to drinking water for the last 2 days before slaughter. Cecal contents were subjected to bacterial analysis. Significant (P < 0.05) Salmonella Typhimurium reductions (ca. 2 log units) relative to levels for untreated control broilers were observed for broilers receiving ECP in combination with SN. The ECP-only treatment resulted in significant (P < 0.05) reductions (ca. 0.8 log) of Salmonella Typhimurium in trial 2. We hypothesize that increasing Salmonella Typhimurium nitrate reductase activity resulted in increased enzymatic reduction of chlorate to chlorite, with a concomitant decrease in cecal Salmonella Typhimurium levels. On the basis of these results, preadaptation with SN followed by ECP supplementation immediately preharvest could be a potential strategy for the reduction of Salmonella Typhimurium in broilers.


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