An investigation of oxidant/antioxidant balance in patients with migraine: a case-control study

Abstract Background In recent years, the role of neuroinflammation and oxidative stress in migraine pathogenesis has achieved considerable interest; however, to date findings are equivocal. Thus, the objective of this study was to investigate biomarkers of oxidative stress in episodic and chronic migraineurs (EM and CM patients) and controls. Methods Forty-four patients with EM, 27 individuals with CM and 19 age-sex-matched controls were enrolled. After collecting data on demographic and headache characteristics, blood samples were collected and analyzed to detect serum levels of oxidative stress biomarkers (malondialdehyde (MDA) and nitric oxide (NO)); total antioxidant capacity using Trolox equivalent antioxidant capacity (TEAC) assay; and antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase-1 (GPx-1)). Results Serum levels of CAT and SOD were significantly lower in the CM group than the EM group and controls. However, serum GPx-1 levels of the CM patients were slightly higher than the EM patients and controls (P-value≤0.001). CM patients had lower mean TEAC values than EM patients and controls. In addition, serum levels of NO and MDA were significantly elevated among subjects with CM compared to EM and control individuals (P-value≤0.001). Pearson correlation analysis revealed negative correlations between the number of days of having headaches per month and serum concentrations of the two antioxidant enzymes CAT (r = − 0.60, P-value< 0.001) and SOD (r = − 0.50, P-value< 0.001) as well as TEAC values (r = − 0.61, P-value< 0.001); however, there were positive correlations between headache days and serum GPx-1 levels (r = 0.46, P-value< 0.001), NO (r = 0.62, P-value< 0.001), and MDA (r = 0.64, P-value< 0.001). Conclusion Present findings highlighted that chronic migraineurs had lower total non-enzymatic antioxidant capacity and higher oxidative stress than episodic migraineurs and control individuals. Although more studies are needed to confirm these data, applying novel prophylactic medications or dietary supplements with antioxidant properties could be promising in migraine therapy.

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Oxidative stress and outcome of antioxidant supplementation in patients with polycystic ovarian syndrome (PCOS)

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20181892 ◽

2018 ◽

Vol 7 (5) ◽

pp. 1667 ◽

Cited By ~ 5

Author(s):

Abubakar A. Panti ◽

Constance E. Shehu ◽

Yusuf Saidu ◽

Karima A. Tunau ◽

Emmanuel I. Nwobodo ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Serum Levels ◽

Clinical Pregnancy ◽

Oxidative Stress Marker ◽

Randomised Control Trial ◽

Antioxidant Supplementation ◽

Control Groups ◽

Stress Marker ◽

And Control

Background: PCOS is a condition with significant decrease in antioxidant with an increased risk of oxidative stress. Antioxidant supplementation has been shown to improve insulin sensitivity in PCOS and may improve outcome of management of PCOS. Objectives of this study were to determine the oxidative stress level of PCOS patients, to assess the effect of antioxidant supplementation on the outcome of management of PCOS and to compare with a control group.Methods: The study was a single blind randomised control trial involving 200 patients with PCOS. The study was conducted at Usmanu Danfodiyo University Teaching Hospital Sokoto. They were randomly divided into intervention and control groups and base line serum levels of oxidative stress marker, antioxidant enzymes, vitamins and minerals were determined. Antioxidant supplementation and placebo were given to the intervention and control groups respectively. All the patients had ovulation induction with clomiphene citrate and were followed up for 6 months. Outcome measures were clinical pregnancy or menstrual regularisation. Level of significance was <0.05.Results: There was statistical significance in the serum levels of oxidative stress marker, antioxidant enzymes, vitamins and minerals between the two groups (post intervention). Glutathione peroxidase (p = 0.001), superoxide dismutase (p = 0.0001), catalase (p= 0.0369), melondialdehyde (p= 0.007), Vitamin A, Vitamin C, Vitamin E (p = 0.0001), zinc and copper (p = 0.0001). The clinical pregnancy outcomes were 22 (22%) versus 2 (2%); live births 18 (18%) versus 2 (2%) and menstrual regularisation 48 (48%) versus 46 (46%) in the intervention and control groups respectively.Conclusions: Antioxidant supplementation in this study significantly affected pregnancy rate in patients with PCOS. Larger studies are suggested to revisit the conclusion of the Cochrane review that antioxidants supplementation had no significant role in female infertility.

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Effect of the French Oak Wood Extract Robuvit on Markers of Oxidative Stress and Activity of Antioxidant Enzymes in Healthy Volunteers: A Pilot Study

Oxidative Medicine and Cellular Longevity ◽

10.1155/2014/639868 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 9

Author(s):

Martina Horvathova ◽

Zuzana Orszaghova ◽

Lucia Laubertova ◽

Magdalena Vavakova ◽

Peter Sabaka ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Antioxidant Capacity ◽

Healthy Volunteers ◽

Total Antioxidant Capacity ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Antioxidant ◽

Trolox Equivalent ◽

Markers Of Oxidative Stress

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.

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Oral Quercetin Supplementation and Blood Oxidative Capacity in Response to Ultramarathon Competition

International Journal of Sport Nutrition and Exercise Metabolism ◽

10.1123/ijsnem.18.6.601 ◽

2008 ◽

Vol 18 (6) ◽

pp. 601-616 ◽

Cited By ~ 32

Author(s):

John C. Quindry ◽

Steven R. McAnulty ◽

Matthew B. Hudson ◽

Peter Hosick ◽

Charles Dumke ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Damage ◽

Antioxidant Capacity ◽

Aqueous Phase ◽

Antioxidant Properties ◽

Oxidative Capacity ◽

Protein Carbonyls ◽

Trolox Equivalent Antioxidant Capacity ◽

Double Blind ◽

Reducing Ability

Previous research indicates that ultramarathon exercise can result in blood oxidative stress. The purpose of this investigation was to examine the efficacy of oral supplementation with quercetin, a naturally occurring compound with known antioxidant properties, as a potential countermeasure against blood oxidative stress during an ultramarathon competition. In double-blind fashion, 63 participants received either oral quercetin (250 mg, 4×/day; 1,000 mg/day total) or quercetin-free supplements 3 weeks before and during the 160-km Western States Endurance Run. Blood drawn before and immediately after (quercetin finishers n = 18, quercetin-free finishers n = 21) the event was analyzed for changes in blood redox status and oxidative damage. Results show that quercetin supplementation did not affect race performance. In response to the ultramarathon challenge, aqueous-phase antioxidant capacity (ferric-reducing ability of plasma) was similarly elevated in athletes in both quercetin and quercetin-free treatments and likely reflects significant increases in plasma urate levels. Alternatively, trolox-equivalent antioxidant capacity was not altered by exercise or quercetin. Accordingly, neither F2-isoprostances nor protein carbonyls were influenced by either exercise or quercetin supplementation. In the absence of postrace blood oxidative damage, these findings suggest that oral quercetin supplementation does not alter blood plasma lipid or aqueous-phase antioxidant capacity or oxidative damage during an ultramarathon challenge.

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The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs

BMC Veterinary Research ◽

10.1186/s12917-021-02882-1 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Sina Salavati ◽

Asghar Mogheiseh ◽

Saeed Nazifi ◽

Atefeh Amiri ◽

Behrooz Nikahval

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Antioxidant Properties ◽

Mature Female ◽

Control Group ◽

Melatonin Treatment ◽

Melatonin Administration ◽

Before And After ◽

Commercial Kits ◽

And Control

Abstract Background As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce oxidative stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on oxidative stress in dogs before and after OHE. In this study, 25 mature female intact dogs were selected and randomly divided into five equal groups: Melatonin (melatonin, no surgery), OHE (no melatonin, surgery), OHE + melatonin (melatonin, surgery), anesthesia+melatonin (melatonin, sham surgery), and control (no melatonin, no surgery) groups. Melatonin (0.3 mg/Kg/day, p.o.) was administrated to the dogs in the melatonin, OHE + melatonin, and anesthesia+melatonin groups on days − 1, 0, 1, 2, and 3 (day 0 = OHE). Blood sampling was performed on days − 1, 1, 3, and 5 of the study. Blood samples were immediately transferred to the laboratory and sera were separated and stored at − 20 °C. Superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and malondialdehyde (MDA) concentrations were measured with commercial kits. Results The levels of SOD, GPX and CAT were significantly higher in the melatonin and anesthesia+melatonin groups compared to those of the control group at days 3 and 5. The level of antioxidant enzymes significantly decreased in the OHE group compared to that of other groups at days 3 and 5. The administration of melatonin increased the level of antioxidant enzymes in ovariohysterectomized dogs. Ovariohysterectomy significantly increased the concentration of MDA in comparison to that of other groups at day 3. Melatonin administration significantly decreased the level of MDA in melatonin, anesthetized, and ovariohysterectomized dogs at day 3. Conclusions Administration of melatonin on day − 1, 0, 1, 2 and 3 modulate the oxidative stress induced by OHE in dogs by increasing antioxidant enzymes concentration and decreasing MDA levels.

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The Effects of Melatonin Treatment on Oxidative Stress Induced by Ovariohysterectomy in Dogs

10.21203/rs.3.rs-123019/v1 ◽

2020 ◽

Author(s):

Sina Salavati ◽

Asghar Mogheiseh ◽

Saeed Nazifi ◽

Atefeh Amiri ◽

Behrooz Nikahval

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Antioxidant Properties ◽

Mature Female ◽

Control Group ◽

Control Groups ◽

Melatonin Treatment ◽

Melatonin Administration ◽

Before And After ◽

And Control

Abstract Background: As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on oxidative stress in dogs before and after OHE. In this study, 25 mature female intact dogs were selected and randomly divided into five equal groups: Melatonin, OHE, melatonin+OHE+melatonin, melatonin+anesthesia+melatonin, and control groups. Melatonin (0.3 mg/Kg/day, p.o.) was administrated to the dogs in the melatonin, melatonin+OHE+melatonin, and melatonin+anesthesia+melatonin groups on days -1, 1, 2, and 3 (day 0=OHE). Blood sampling was performed on days -1, 1, 3, and 5 of the study. Blood samples were immediately transferred to the laboratory and sera were separated and stored at -20 °C. Superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) concentrations were significantly higher in the melatonin and melatonin+anesthesia+melatonin groups compared to those of the control group. Results: The level of antioxidant enzymes significantly decreased in the OHE group compared to that of other groups. The administration of melatonin increased the level of antioxidant enzymes in ovariohysterectomized dogs. OHE significantly increased the concentration of malondialdehyde (MDA) in comparison to that of other groups. Melatonin administration significantly decreased the level of MDA in intact, anesthetized, and ovariohysterectomized dogs. Conclusions: It can be stated that the administration of melatonin one day before and during one week after OHE could control oxidative stress in dogs with increased antioxidant enzymes and decreased MDA levels.

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Isolation and structural elucidation of dimeric epigallocatechin-3-gallate autoxidation products and their antioxidant capacity

European Food Research and Technology ◽

10.1007/s00217-021-03846-3 ◽

2021 ◽

Author(s):

Julian Alfke ◽

Uta Kampermann ◽

Svetlana Kalinina ◽

Melanie Esselen

Keyword(s):

Antioxidant Capacity ◽

Antioxidant Properties ◽

Cd Spectroscopy ◽

Trolox Equivalent Antioxidant Capacity ◽

Published Data ◽

Dietary Polyphenols ◽

Cellular Effects ◽

The Impact

AbstractDietary polyphenols like epigallocatechin-3-gallate (EGCG)—which represents the most abundant flavan-3-ol in green tea—are subject of several studies regarding their bioactivity and health-related properties. On many occasions, cell culture or in vitro experiments form the basis of published data. Although the stability of these compounds is observed to be low, many reported effects are directly related to the parent compounds whereas the impact of EGCG degradation and autoxidation products is not yet understood and merely studied. EGCG autoxidation products like its dimers theasinensin A and D, “P2” and oolongtheanin are yet to be characterized in the same extent as their parental polyphenol. However, to investigate the bioactivity of autoxidation products—which would minimize the discrepancy between in vitro and in vivo data—isolation and structure elucidation techniques are urgently needed. In this study, a new protocol to acquire the dimers theasinensin A and D as well as oolongtheanin is depicted, including a variety of spectroscopic and quadrupole time-of-flight high-resolution mass spectrometric (qTOF-HRMS) data to characterize and assign these isolates. Through nuclear magnetic resonance (NMR) spectroscopy, polarimetry, and especially circular dichroism (CD) spectroscopy after enzymatic hydrolysis the complementary atropisomeric stereochemistry of the isolated theasinensins is illuminated and elucidated. Lastly, a direct comparison between the isolated EGCG autoxidation products and the monomer itself is carried out regarding their antioxidant properties featuring Trolox equivalent antioxidant capacity (TEAC) values. These findings help to characterize these products regarding their cellular effects and—which is of special interest in the flavonoid group—their redox properties.

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Abstract MP58: Tissue And Sex Specific Effects Of Gstm1 Deletion In Mouse Models

Hypertension ◽

10.1161/hyp.78.suppl_1.mp58 ◽

2021 ◽

Vol 78 (Suppl_1) ◽

Author(s):

Yves Wang ◽

Nhu Nguyen ◽

Keith Nehrke ◽

Paul S Brookes ◽

Thu H Le

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Mouse Model ◽

Molecular Mechanisms ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Antioxidant Properties ◽

Iri Model ◽

Increased Risk ◽

Study Gene Expression

The glutathione S-transferase ( Gst ) gene family encodes antioxidant enzymes. In humans, a common null allele deletion variant of GST μ-1 ( GSTM1 ) is highly prevalent across populations and is associated with increased risk and progression of various diseases. Using a Gstm1 knockout (KO) mouse model, we previously showed that KO mice with angiotensin II-induced hypertension (HTN) have increased kidney injury compared to wild-type (WT) controls, mediated by elevated oxidative stress. In the same mouse model, we have recently reported that in a Langendorff-perfused cardiac ischemia-reperfusion injury (IRI) model, where damage is also mediated by oxidative stress, male KO hearts are protected while females are not. Here, we investigated the molecular mechanisms for this difference in male hearts. WT and KO mice of both sexes were studied at 12-20 weeks of age. Hearts were snap frozen at baseline and after 25 min of global ischemia, and kidneys were collected at baseline and 4 weeks following HTN induction. A panel of 18 Gst genes were probed by qPCR from baseline hearts and kidneys of both sexes. Global metabolites were assayed using Metabolon, Inc. from hearts of both sexes and kidneys of males, at both baseline and diseased states. Analysis by qPCR (n = 3/group) showed that male, but not female, KO hearts had upregulation of other Gst s. In contrast, no significant differences between were found in male kidneys. Metabolomics (n = 6/group) detected 695 metabolites in hearts and 926 in kidneys. There were increases in several metabolites in KO vs. WT hearts including those with antioxidant properties. Notably, increases in carnosine and anserine were observed in KO male hearts but not in female hearts, while that of other antioxidant-related metabolites were observed in hearts of both sexes, but not in kidneys. HTN induced significant increases in metabolites in KO vs. WT kidneys in the pathways related to and linking methionine, cysteine, and glutathione, which were not observed in hearts. In this study, gene expression and metabolites suggest that the mechanisms compensating for the loss of GSTM1 are both tissue and sex specific. The resulting differences in antioxidant enzymes and metabolites may explain the unexpected protection for male Gstm1 KO hearts in IRI.

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The Influence of Hyaluronic Acid Adjunctive Therapy of Periodontitis on Salivary Markers of Oxidative Stress: Randomized, Controlled Clinical Trial

Antioxidants ◽

10.3390/antiox11010135 ◽

2022 ◽

Vol 11 (1) ◽

pp. 135

Author(s):

Iwona Olszewska-Czyz ◽

Kristina Kralik ◽

Marin Tota ◽

Jelena Prpic

Keyword(s):

Oxidative Stress ◽

Hyaluronic Acid ◽

Antioxidant Capacity ◽

Adjunctive Therapy ◽

Antioxidant Properties ◽

Periodontal Therapy ◽

Oral Disease ◽

Controlled Clinical Trial ◽

Clinical Attachment Loss ◽

Periodontal Tissues

Periodontitis is a common oral disease affecting the tooth-supporting tissues. Bacteria have been long viewed as the main causative factor in its development; however, many investigations have proved that aberrant immune and inflammatory response and the resulting misbalance between the damage caused by reactive oxygen species and the antioxidant capacity of tissues may be an underlying factor in disease progression that reduces healing potential. The objective of the current trial is to assess the outcomes of the addition of hyaluronic acid (HA) to standard non-surgical periodontal therapy (NST) on some major oxidative stress markers in saliva. HA-based gel designed for dental application was used and the measurements were taken after 3 months. HA adjunctive therapy had a significantly greater increase in markers with antioxidant properties as well as total antioxidant capacity compared to standard NST alone. Furthermore, clinically measured levels of gingival inflammation (bleeding on probing-BOP) and periodontal destruction (clinical attachment loss-CAL) were significantly correlated with these markers, and the correlation was negative. This investigation demonstrates that HA may indeed express antioxidant properties and improve the antioxidant capacity of periodontal tissues, thus improving the prognosis for the teeth and the results of periodontal therapy. Further investigations will be necessary to determine the duration of these effects over time.

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The Prognostic Role of Glutathione and Its Related Antioxidant Enzymes in the Recurrence of Hepatocellular Carcinoma

Nutrients ◽

10.3390/nu13114071 ◽

2021 ◽

Vol 13 (11) ◽

pp. 4071

Author(s):

Yung-Fang Hsiao ◽

Shao-Bin Cheng ◽

Chia-Yu Lai ◽

Hsiao-Tien Liu ◽

Shih-Chien Huang ◽

...

Keyword(s):

Oxidative Stress ◽

Hepatocellular Carcinoma ◽

Antioxidant Enzymes ◽

Prognostic Significance ◽

Tumor Resection ◽

Trolox Equivalent Antioxidant Capacity ◽

Antioxidant Capacities ◽

The Impact ◽

Hcc Recurrence

The imbalance of high oxidative stress and low antioxidant capacities is thought to be a significant cause of the development and progression of hepatocellular carcinoma (HCC). However, the impact of oxidative stress, glutathione (GSH), and its related antioxidant enzymes on the recurrence of HCC has not been investigated. The purpose of this study was to compare the changes to oxidative stress and GSH-related antioxidant capacities before and after tumor resection in patients with HCC recurrence and non-recurrence. We also evaluated the prognostic significance of GSH and its related enzymes in HCC recurrence. This was a cross-sectional and follow-up study. Ninety-two HCC patients who were going to receive tumor resection were recruited. We followed patients’ recurrence and survival status until the end of the study, and then assigned patients into the recurrent or the non-recurrent group. The tumor recurrence rate was 52.2% during the median follow-up period of 3.0 years. Patients had significantly lower plasma malondialdehyde level, but significantly or slightly higher levels of GSH, glutathione disulfide, trolox equivalent antioxidant capacity, glutathione peroxidase (GPx), and glutathione reductase (GR) activities after tumor resection compared to the respective levels before tumor resection in both recurrent and non-recurrent groups. GSH level in HCC tissue was significantly higher than that in adjacent normal tissue in both recurrent and non-recurrent patients. Decreased plasma GPx (HR = 0.995, p = 0.01) and GR (HR = 0.98, p = 0.04) activities before tumor resection, and the increased change of GPx (post—pre-resection) (HR = 1.004, p = 0.03) activity were significantly associated with the recurrence of HCC. These findings suggest there might be a possible application of GPx or GR as therapeutic targets for reducing HCC recurrence.

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Effect of Propofol, Sevoflurane and Desflurane on Systemic Redox Balance

International Journal of Immunopathology and Pharmacology ◽

10.1177/039463200702000316 ◽

2007 ◽

Vol 20 (3) ◽

pp. 585-593 ◽

Cited By ~ 15

Author(s):

G. Cinnella ◽

G. Vendemiale ◽

M. Dambrosio ◽

G. Serviddio ◽

P.L. Pugliese ◽

...

Keyword(s):

Antioxidant Capacity ◽

Antioxidant Properties ◽

Redox Balance ◽

Trolox Equivalent Antioxidant Capacity ◽

Sodium Thiopental ◽

Blood Samples ◽

Plasma Antioxidant Capacity ◽

Trolox Equivalent ◽

Plasma Antioxidant ◽

Group D

We studied the effects of Propofol, Desflurane, and Sevoflurane on the systemic redox balance in patients undergoing laparohysterectomy. We measured blood concentration of glutathione (GSH), plasma antioxidant capacity (Trolox Equivalent Antioxidant Capacity-TEAC), and lipid peroxidation products (malondialdehyde (aMDA) and 4-hydroxynonenal (aHNE) protein adducts). Sixty patients were randomly placed into three groups of twenty people each. In Group P anesthesia was induced with Propofol 2 mg/kg and maintained with 12–10–8 mg/kg/min; in Groups S and D anesthesia was induced with 3 mg/kg Sodium Thiopental and maintained with 2% Sevoflurane and 6% Desflurane, respectively. Blood samples were collected prior to induction (T0bas), 60min and 24h postoperatively (T160' and T224 h). In Group P, GSH increased on T160' (p<0.02) and returned to baseline on T24h, while TEAC remained unmodified; in Groups S, GSH and TEAC decreased on T160' (p<0.01 vs. T0bas, p<0.03 vs. T0bas, respectively); in Group D, on T160' there was a slight decrease of GSH and TEAC. The levels of aMDA slightly decreased throughout the study periods in Group P, increased in Group D, and remained stable in Group S. Propofol showed antioxidant properties, while Sevoflurane and Desflurane seemed to shift the redox balance towards oxidation, yet without inducing overt oxidative damage.

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