Novel mesoporous silica nanocarriers containing gold; a rapid diagnostic tool for tuberculosis

AbstractTuberculosis (TB) is major health concern and reason of deaths from decades to current date. Even though with a lot of advancements, diagnostic techniques, and discovery of standard antibiotics TB remains crucial challenge and can create worst scenario for human health in near future. Nanoparticles play emerging role in diagnosis and treatment of TB. In this study, we developed mesoporous silica nanoparticles containing gold (MSNs@GNPs) for rapid diagnosis and treatment of TB. The physicochemical characterization revealed effective surface morphology and particles diameter, that is applicable for in vitro applications. The in vitro antimicrobial analysis revealed that the designed MSNs@GNPs has retained significantly lower minimal inhibitory concentration (MIC) values and can effectively demolish mycobacterium tuberculosis (Mtb). Furthermore, the diagnosis efficiency of the MSNs@GNPs was evaluated by calorimetric analysis. Which demonstrates that MSNs@GNPs can be used for rapid diagnosis of the tuberculosis when applied on in vitro culture of the Mtb. The current study needs further verification on human’s clinical samples from tuberculosis patients. However, MSNs@GNPs can be a versatile clinical approach for the rapid diagnosis and clinical treatment of the tuberculosis.

Download Full-text

In Vitro Evaluation of pH Responsive Doxazosin Loaded Mesoporous Silica Nanoparticles: A Smart Approach in Drug Delivery

Current Drug Delivery ◽

10.2174/1567201812666150722123704 ◽

2016 ◽

Vol 13 (4) ◽

pp. 574-581 ◽

Cited By ~ 3

Author(s):

Arijit Guha ◽

Nikhil Biswas ◽

Kaustav Bhattacharjee ◽

Piu Das ◽

Ketousetuo Kuotsu

Keyword(s):

Drug Delivery ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Ph Responsive ◽

In Vitro Evaluation

Download Full-text

Mesoporous Silica Nanoparticles and Mesoporous Bioactive Glasses for Wound Management: From Skin Regeneration to Cancer Therapy

Materials ◽

10.3390/ma14123337 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3337

Author(s):

Sara Hooshmand ◽

Sahar Mollazadeh ◽

Negar Akrami ◽

Mehrnoosh Ghanad ◽

Ahmed El-Fiqi ◽

...

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Skin Regeneration ◽

Bioactive Molecules ◽

Wound Management ◽

Bioactive Glasses ◽

Wide Range

Exploring new therapies for managing skin wounds is under progress and, in this regard, mesoporous silica nanoparticles (MSNs) and mesoporous bioactive glasses (MBGs) offer great opportunities in treating acute, chronic, and malignant wounds. In general, therapeutic effectiveness of both MSNs and MBGs in different formulations (fine powder, fibers, composites etc.) has been proved over all the four stages of normal wound healing including hemostasis, inflammation, proliferation, and remodeling. The main merits of these porous substances can be summarized as their excellent biocompatibility and the ability of loading and delivering a wide range of both hydrophobic and hydrophilic bioactive molecules and chemicals. In addition, doping with inorganic elements (e.g., Cu, Ga, and Ta) into MSNs and MBGs structure is a feasible and practical approach to prepare customized materials for improved skin regeneration. Nowadays, MSNs and MBGs could be utilized in the concept of targeted therapy of skin malignancies (e.g., melanoma) by grafting of specific ligands. Since potential effects of various parameters including the chemical composition, particle size/morphology, textural properties, and surface chemistry should be comprehensively determined via cellular in vitro and in vivo assays, it seems still too early to draw a conclusion on ultimate efficacy of MSNs and MBGs in skin regeneration. In this regard, there are some concerns over the final fate of MSNs and MBGs in the wound site plus optimal dosages for achieving the best outcomes that deserve careful investigation in the future.

Download Full-text

Antimicrobial and antibiofilm activities of meropenem loaded-mesoporous silica nanoparticles against carbapenem-resistant Pseudomonas aeruginosa

Journal of Biomaterials Applications ◽

10.1177/08853282211003848 ◽

2021 ◽

pp. 088532822110038

Author(s):

Mohammad Yousef Memar ◽

Mina Yekani ◽

Hadi Ghanbari ◽

Edris Nabizadeh ◽

Sepideh Zununi Vahed ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Carbapenem Resistant ◽

Toxicity In Vitro ◽

Different Levels

The aims of the present study were the determination of antimicrobial and antibiofilm effects of meropenem-loaded mesoporous silica nanoparticles (MSNs) on carbapenem resistant Pseudomonas aeruginosa ( P. aeruginosa) and cytotoxicity properties in vitro. The meropenem-loaded MSNs had shown antibacterial and biofilm inhibitory activities on all isolates at different levels lower than MICs and BICs of meropenem. The viability of HC-04 cells treated with serial concentrations as MICs and BICs of meropenem-loaded MSNs was 92–100%. According to the obtained results, meropenem-loaded MSNs display the significant antibacterial and antibiofilm effects against carbapenem resistant and biofilm forming P. aeruginosa and low cell toxicity in vitro. Then, the prepared system can be an appropriate option for the delivery of carbapenem for further evaluation in vivo assays.

Download Full-text

Polymeric Mesoporous Silica Nanoparticles for Enhanced Delivery of 5-Fluorouracil In Vitro

Pharmaceutics ◽

10.3390/pharmaceutics11060288 ◽

2019 ◽

Vol 11 (6) ◽

pp. 288 ◽

Cited By ~ 19

Author(s):

Thashini Moodley ◽

Moganavelli Singh

Keyword(s):

Side Effects ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Therapeutic Potential ◽

Mesoporous Silica Nanoparticles ◽

Drug Loading ◽

Hek293 Cells ◽

Cancer Drug ◽

Delivery Vehicles

There is a need for the improvement of conventional cancer treatment strategies by incorporation of targeted and non-invasive procedures aimed to reduce side-effects, drug resistance, and recurrent metastases. The anti-cancer drug, 5-fluorouracil (5-FU), is linked to a variety of induced-systemic toxicities due to its lack of specificity and potent administration regimens, necessitating the development of delivery vehicles that can enhance its therapeutic potential, while minimizing associated side-effects. Polymeric mesoporous silica nanoparticles (MSNs) have gained popularity as delivery vehicles due to their high loading capacities, biocompatibility, and good pharmacokinetics. MSNs produced in this study were functionalized with the biocompatible polymers, chitosan, and poly(ethylene)glycol to produce monodisperse NPs of 36–65 nm, with a large surface area of 710.36 m2/g, large pore volume, diameter spanning 9.8 nm, and a favorable zeta potential allowing for stability and enhanced uptake of 5-FU. Significant drug loading (0.15–0.18 mg5FU/mgmsn), controlled release profiles (15–65%) over 72 hours, and cell specific cytotoxicity in cancer cells (Caco-2, MCF-7, and HeLa) with reduced cell viability (≥50%) over the non-cancer (HEK293) cells were established. Overall, these 5FU-MSN formulations have been shown to be safe and effective delivery systems in vitro, with potential for in vivo applications.

Download Full-text

Design considerations for mesoporous silica nanoparticulate systems in facilitating biomedical applications

Open Material Sciences ◽

10.2478/mesbi-2014-0001 ◽

2014 ◽

Vol 1 (1) ◽

Cited By ~ 26

Author(s):

Diti Desai ◽

Didem Sen Karaman ◽

Neeraj Prabhakar ◽

Sina Tadayon ◽

Alain Duchanoy ◽

...

Keyword(s):

Mesoporous Silica ◽

Biomedical Applications ◽

Common Ground ◽

Particle System ◽

Mesoporous Silica Nanoparticles ◽

Cellular Internalization ◽

Design Considerations ◽

Design Options ◽

Nanoparticulate Systems

AbstractMesoporous silica nanoparticles (MSNs) have advanced to the forefront of multifunctional nanoparticulate systems in nanomedicine, owing to this highly fexible materials platform enabling a multitude of design options, often in a modular fashion. Drug delivery ability, detectability via diferent imaging modalities, and stimuliresponsiveness are often combined into one particle system. Very sophisticated and versatile designs along with impressive demonstrations of applicability have been reported to date, but a common ground when it comes to some critical considerations valid for any nanoparticle intended for biomedical purposes is lacking to some degree. In this study, we attempt to take a glance at some of the most crucial aspects of biomedical nanoparticulate design and relate how they apply specifically toMSNs. These considerations include fuorophore labeling and leaching with respect to immobilization to MSNs, the surrounding conditions, carrier biodegradability, and surface coating. Surface modifcation strategies and surface charge tuning are further considered in conjunction to the relative amount of cellular uptake and serum protein adsorption. Cellular internalization routes and biological techniques used to evaluate especially in vitro biobehavior are discussed. Our attempt is hereby to draw attention to some of the most frequently occurring issues to be considered in the design of MSN systems for biomedical applications

Download Full-text

pH-Responsive Release of Ruthenium Metallotherapeutics from Mesoporous Silica-Based Nanocarriers

Pharmaceutics ◽

10.3390/pharmaceutics13040460 ◽

2021 ◽

Vol 13 (4) ◽

pp. 460

Author(s):

Minja Mladenović ◽

Ibrahim Morgan ◽

Nebojša Ilić ◽

Mohamad Saoud ◽

Marija V. Pergal ◽

...

Keyword(s):

Drug Delivery ◽

Mesoporous Silica ◽

Inductively Coupled Plasma ◽

Drug Delivery Systems ◽

Mesoporous Silica Nanoparticles ◽

Release Kinetics ◽

Delivery Systems ◽

Emission Spectrometry ◽

Ph Responsive

Ruthenium complexes are attracting interest in cancer treatment due to their potent cytotoxic activity. However, as their high toxicity may also affect healthy tissues, efficient and selective drug delivery systems to tumour tissues are needed. Our study focuses on the construction of such drug delivery systems for the delivery of cytotoxic Ru(II) complexes upon exposure to a weakly acidic environment of tumours. As nanocarriers, mesoporous silica nanoparticles (MSN) are utilized, whose surface is functionalized with two types of ligands, (2-thienylmethyl)hydrazine hydrochloride (H1) and (5,6-dimethylthieno[2,3-d]pyrimidin-4-yl)hydrazine (H2), which were attached to MSN through a pH-responsive hydrazone linkage. Further coordination to ruthenium(II) center yielded two types of nanomaterials MSN-H1[Ru] and MSN-H2[Ru]. Spectrophotometric measurements of the drug release kinetics at different pH (5.0, 6.0 and 7.4) confirm the enhanced release of Ru(II) complexes at lower pH values, which is further supported by inductively coupled plasma optical emission spectrometry (ICP-OES) measurements. Furthermore, the cytotoxicity effect of the released metallotherapeutics is evaluated in vitro on metastatic B16F1 melanoma cells and enhanced cancer cell-killing efficacy is demonstrated upon exposure of the nanomaterials to weakly acidic conditions. The obtained results showcase the promising capabilities of the designed MSN nanocarriers for the pH-responsive delivery of metallotherapeutics and targeted treatment of cancer.

Download Full-text