Polymeric Mesoporous Silica Nanoparticles for Enhanced Delivery of 5-Fluorouracil In Vitro

There is a need for the improvement of conventional cancer treatment strategies by incorporation of targeted and non-invasive procedures aimed to reduce side-effects, drug resistance, and recurrent metastases. The anti-cancer drug, 5-fluorouracil (5-FU), is linked to a variety of induced-systemic toxicities due to its lack of specificity and potent administration regimens, necessitating the development of delivery vehicles that can enhance its therapeutic potential, while minimizing associated side-effects. Polymeric mesoporous silica nanoparticles (MSNs) have gained popularity as delivery vehicles due to their high loading capacities, biocompatibility, and good pharmacokinetics. MSNs produced in this study were functionalized with the biocompatible polymers, chitosan, and poly(ethylene)glycol to produce monodisperse NPs of 36–65 nm, with a large surface area of 710.36 m2/g, large pore volume, diameter spanning 9.8 nm, and a favorable zeta potential allowing for stability and enhanced uptake of 5-FU. Significant drug loading (0.15–0.18 mg5FU/mgmsn), controlled release profiles (15–65%) over 72 hours, and cell specific cytotoxicity in cancer cells (Caco-2, MCF-7, and HeLa) with reduced cell viability (≥50%) over the non-cancer (HEK293) cells were established. Overall, these 5FU-MSN formulations have been shown to be safe and effective delivery systems in vitro, with potential for in vivo applications.

Download Full-text

Therapeutic Potential of Polymer-Coated Mesoporous Silica Nanoparticles

Applied Sciences ◽

10.3390/app10010289 ◽

2019 ◽

Vol 10 (1) ◽

pp. 289 ◽

Cited By ~ 4

Author(s):

Kuldeep K. Bansal ◽

Deepak K. Mishra ◽

Ari Rosling ◽

Jessica M. Rosenholm

Keyword(s):

Drug Delivery ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Therapeutic Potential ◽

Mesoporous Silica Nanoparticles ◽

Drug Loading ◽

Polymer Coatings ◽

Drug Delivery Carrier ◽

Great Solution ◽

Selection Of

Mesoporous silica nanoparticles (MSNs) find tremendous applications in drug delivery due to several advantages such as their easy fabrication process, high drug loading, biodegradability, biocompatibility, and so forth. Nevertheless, despite several advantages, the use of this striking drug delivery carrier is restricted due to premature drug release owing to the porous structure. Coating of the pores using polymers has emerged as a great solution to this problem. Polymer coatings, which act as gatekeepers, avoid the premature release of loaded content from MSNs and offers the opportunity for controlled and targeted drug delivery. Therefore, in this review, we have compiled the polymer-based coating approaches used in recent years for improving the drug delivery capability of MSNs. This manuscript provides an insight into the research about the potential of polymer-coated MSNs, allowing the selection of right polymer for coating purposes according to the desired application.

Download Full-text

Functionalized Mesoporous Silica Nanoparticles as Delivery Systems for Doxorubicin: Drug Loading and Release

Applied Sciences ◽

10.3390/app11136121 ◽

2021 ◽

Vol 11 (13) ◽

pp. 6121

Author(s):

Candace M. Day ◽

Martin J. Sweetman ◽

Yunmei Song ◽

Sally E. Plush ◽

Sanjay Garg

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Release Kinetics ◽

Drug Loading ◽

In Vitro Release ◽

Delivery Systems ◽

Stimuli Responsive ◽

Kinetics Of

Functionalized nanoparticles have played a major role in the field of targeted therapy, owing to their ability to control the release and for the selective delivery of entrapped materials to tumours. In this work, we described the loading capacity and in vitro release kinetics of mesoporous silica nanoparticles (MSNs), functionalized with Poly-L-Histidine and Tamoxifen. The model drug Doxorubicin (DOX) was successfully encapsulated into MSN-based systems, using the technique of solvent immersion. A post-surface grafting loading method was investigated on functionalized systems, with DOX loading content determined using HPLC. Dialysis bag diffusion was employed to investigate the release kinetics of DOX-loaded-systems at pH 7.4 and 5. The amount of DOX released from native MSNs systems over a 72 h period at pH 5 was approximately 40%; and at pH 7.4 ≈ 30%. A moderate pH dependent release behaviour was observed with both our functionalized systems: DOX@MSN-PLH and DOX@MSN-PLH-TAM; with approximately 5% of DOX released from DOX@MSN-PLH-TAM at pH 7.4 and about 9% released at pH 7.4 over 72 h. The maximal cumulated release of DOX molecules from DOX@MSN-PLH after 72 h was ≈ 18% at pH 7.4 and ≈ 23% at pH 5, respectively. The outcome of this work offers a promising contribution towards building future stimuli-responsive nano-drug delivery systems.

Download Full-text

Erythrocyte-cancer hybrid membrane-camouflaged mesoporous silica nanoparticles loaded with Gboxin for glioma-targeting therapy

Current Pharmaceutical Biotechnology ◽

10.2174/1389201022666210719164538 ◽

2021 ◽

Vol 22 ◽

Author(s):

Xiuxiu Jiao ◽

Xiaoyan Yu ◽

Chunai Gong ◽

Hao Zhu ◽

Bin Zhang ◽

...

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Delivery System ◽

Cell Model ◽

Mesoporous Silica Nanoparticles ◽

Drug Loading ◽

Circulation System ◽

Normal Cells ◽

Self Recognition

Objective: The purpose of this research is to formulate a biomimetic drug delivery system that can selectively target glioblastoma (GBM) to deliver the antitumor agent, Gboxin: a novel Complex V inhibitor. Gboxin can specifically inhibit GBM cell growth but not normal cells. Methods: In the present study, we utilized Red Blood Cell (RBC) membrane and U251 cell membrane to obtain a hybrid biomimetic membrane (RBC-U), and prepared RBC-U coated Gboxin-loaded mesoporous silica nanoparticles ((MSNs/Gboxin)@[RBC-U]) for GBM chemotherapy. The zeta potential, particle size, and morphology of (MSNs/Gboxin)@[RBC-U] were characterized. The cellular uptake, effect of cells growth inhibition, biocompatibility, and specific self-recognition of nanoparticles were evaluated. Results: The (MSNs/Gboxin)@[RBC-U] was successfully fabricated and possessed high stability in the circulation system. The drug loading of Gboxin was 13.9%. (MSNs/Gboxin)@[RBC-U], effectively retain drugs in the physiological environment and releasing Gboxin rapidly in the tumor cells. Compared to the MSNs/Gboxin, the (MSNs/Gboxin)@[RBC-U] exhibited highly specific self-recognition to the source cell line. Additionally, the (MSNs/Gboxin)@[RBC-U] showed excellent anti-proliferation efficiency (IC50 = 0.21 μg/mL) in the tumor cell model and a few side effects in normal cells in vitro. Conclusion: The (MSNs/Gboxin)@[RBC-U] exhibited significant anti-cancer effects in vitro and the specific self-recognition to GBM cells. Hence, (MSNs/Gboxin)@[RBC-U] could be a promising delivery system for GBM targeted therapy.

Download Full-text

Preparation of Mesoporous Silica Nanoparticles for Insulin Drug Delivery

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.829.251 ◽

2013 ◽

Vol 829 ◽

pp. 251-257 ◽

Cited By ~ 3

Author(s):

Abdollah Zakeri Siavashani ◽

Masoume Haghbin Nazarpak ◽

Fateme Fayyaz Bakhsh ◽

Tayebeh Toliyat ◽

Mehran Solati-Hashjin

Keyword(s):

Drug Delivery ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Size Distribution ◽

Mesoporous Silica Nanoparticles ◽

Drug Carrier ◽

Drug Loading ◽

Hydrothermal Process ◽

Analytical Techniques

This study has focused on mesoporous silica nanoparticles as a drug delivery system of insulin, which was synthesized via a hydrothermal process. The morphology and composition of the silica nanoparticles were characterized by different analytical techniques such as Scanning Electron Microscope (SEM), X-Ray Diffraction Analysis (XRD), Fourier Transform Infrared spectroscopy (FTIR) and BrunauerEmmettTeller (BET). The percentage of drug loading and the in vitro drug release properties of the mesoporous silica nanoparticles in gastrointestinal tract were investigated in simulated gastrointestinal conditions by ultraviolet-visible spectroscopy. The results showed the amorphous structure of SBA-15 in mesoporous silica particles has a narrow pore size distribution. Also, particles shape was nearly wheat-like with almost homogeneous size distribution. Furthermore, it was revealed that the mesoporous silica nanoparticles have a high insulin loading and release capacity. These prominent behaviors make mesoporous silica nanoparticles promising material as a drug carrier for insulin delivery.

Download Full-text

In Vitro Evaluation of pH Responsive Doxazosin Loaded Mesoporous Silica Nanoparticles: A Smart Approach in Drug Delivery

Current Drug Delivery ◽

10.2174/1567201812666150722123704 ◽

2016 ◽

Vol 13 (4) ◽

pp. 574-581 ◽

Cited By ~ 3

Author(s):

Arijit Guha ◽

Nikhil Biswas ◽

Kaustav Bhattacharjee ◽

Piu Das ◽

Ketousetuo Kuotsu

Keyword(s):

Drug Delivery ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Ph Responsive ◽

In Vitro Evaluation

Download Full-text

Mesoporous Silica Nanoparticles and Mesoporous Bioactive Glasses for Wound Management: From Skin Regeneration to Cancer Therapy

Materials ◽

10.3390/ma14123337 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3337

Author(s):

Sara Hooshmand ◽

Sahar Mollazadeh ◽

Negar Akrami ◽

Mehrnoosh Ghanad ◽

Ahmed El-Fiqi ◽

...

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Skin Regeneration ◽

Bioactive Molecules ◽

Wound Management ◽

Bioactive Glasses ◽

Wide Range

Exploring new therapies for managing skin wounds is under progress and, in this regard, mesoporous silica nanoparticles (MSNs) and mesoporous bioactive glasses (MBGs) offer great opportunities in treating acute, chronic, and malignant wounds. In general, therapeutic effectiveness of both MSNs and MBGs in different formulations (fine powder, fibers, composites etc.) has been proved over all the four stages of normal wound healing including hemostasis, inflammation, proliferation, and remodeling. The main merits of these porous substances can be summarized as their excellent biocompatibility and the ability of loading and delivering a wide range of both hydrophobic and hydrophilic bioactive molecules and chemicals. In addition, doping with inorganic elements (e.g., Cu, Ga, and Ta) into MSNs and MBGs structure is a feasible and practical approach to prepare customized materials for improved skin regeneration. Nowadays, MSNs and MBGs could be utilized in the concept of targeted therapy of skin malignancies (e.g., melanoma) by grafting of specific ligands. Since potential effects of various parameters including the chemical composition, particle size/morphology, textural properties, and surface chemistry should be comprehensively determined via cellular in vitro and in vivo assays, it seems still too early to draw a conclusion on ultimate efficacy of MSNs and MBGs in skin regeneration. In this regard, there are some concerns over the final fate of MSNs and MBGs in the wound site plus optimal dosages for achieving the best outcomes that deserve careful investigation in the future.

Download Full-text

Antimicrobial and antibiofilm activities of meropenem loaded-mesoporous silica nanoparticles against carbapenem-resistant Pseudomonas aeruginosa

Journal of Biomaterials Applications ◽

10.1177/08853282211003848 ◽

2021 ◽

pp. 088532822110038

Author(s):

Mohammad Yousef Memar ◽

Mina Yekani ◽

Hadi Ghanbari ◽

Edris Nabizadeh ◽

Sepideh Zununi Vahed ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Carbapenem Resistant ◽

Toxicity In Vitro ◽

Different Levels

The aims of the present study were the determination of antimicrobial and antibiofilm effects of meropenem-loaded mesoporous silica nanoparticles (MSNs) on carbapenem resistant Pseudomonas aeruginosa ( P. aeruginosa) and cytotoxicity properties in vitro. The meropenem-loaded MSNs had shown antibacterial and biofilm inhibitory activities on all isolates at different levels lower than MICs and BICs of meropenem. The viability of HC-04 cells treated with serial concentrations as MICs and BICs of meropenem-loaded MSNs was 92–100%. According to the obtained results, meropenem-loaded MSNs display the significant antibacterial and antibiofilm effects against carbapenem resistant and biofilm forming P. aeruginosa and low cell toxicity in vitro. Then, the prepared system can be an appropriate option for the delivery of carbapenem for further evaluation in vivo assays.

Download Full-text