scholarly journals d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Satya Narayan Patel ◽  
Girija Kaushal ◽  
Sudhir P. Singh
Keyword(s):  
Large Scale ◽  
Probiotic Strain ◽  
Heat Stability ◽  
Yeast Fermentation ◽  
Scale Production ◽  
The Novel ◽  
Turnover Number ◽  
High Turnover ◽  
Excessive Heat ◽  
Large Scale Production

Abstract Background d-Allulose is an ultra-low calorie sugar of multifarious health benefits, including anti-diabetic and anti-obesity potential. d-Allulose 3-epimerase family enzymes catalyze biosynthesis of d-allulose via epimerization of d-fructose. Results A novel d-allulose 3-epimerase (DaeB) was cloned from a plant probiotic strain, Bacillus sp. KCTC 13219, and expressed in Bacillus subtilis cells. The purified protein exhibited substantial epimerization activity in a broad pH spectrum, 6.0–11.0. DaeB was able to catalyze d-fructose to d-allulose bioconversion at the temperature range of 35 °C to 70 °C, exhibiting at least 50 % activity. It displaced excessive heat stability, with the half-life of 25 days at 50 °C, and high turnover number (kcat 367 s− 1). The coupling of DaeB treatment and yeast fermentation of 700 g L− 1d-fructose solution yielded approximately 200 g L− 1d-allulose, and 214 g L− 1 ethanol. Conclusions The novel d-allulose 3-epimerase of Bacillus sp. origin discerned a high magnitude of heat stability along with exorbitant epimerization ability. This biocatalyst has enormous potential for the large-scale production of d-allulose.

scholarly journals Isolation and Characterization of PHA Producing Bacteria from Sewage Samples of Assam

2019 ◽  
Vol 8 (4) ◽  
pp. 10686-10692
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Bacterial Species ◽  
Scale Production ◽  
High Turnover ◽  
Isolation And Characterization ◽  
Naturally Occurring ◽  
Large Scale Production ◽  
The Individual

Polyhydroxyalkanoate (Pha) Is A Family Of Naturally Occurring Polymers Which Is Reportedly Produced By More Than 75 Genera Of Gram-Positive And Gram-Negative Bacteria. Although The Industrial Production Of This Bioplastic Commenced Decades Ago, Its Large Scale Production Is Still Debilitated By Factors Such As Rate Of Production By The Bacteria And The Raw Materials For Its Production, Which Finally Elevates The Production Cost. The Present Thrust In This Area Is To Isolate Bacterial Species Which Have A High Turnover Of Pha With Minor Expenditure. Sewage And Sewage Sludge Samples Are Rich Sources Of Microflora Which Offer A Nutrient Limited Habitat To The Individual Organisms. Under These Conditions Of Stress, Native Organisms Develop A Metabolomics Which Efficiently Convert Vital Nutrients Into Essential Macromolecules, And In This Case A Storage Polymer Of Industrial Significance I.E. Pha. In This Pilot Study An Attempt Was Made To Isolate Indigenous Bacterial With Ability To Produce The Polymer.

scholarly journals Specialized Metabolites and Valuable Molecules in Crop and Medicinal Plants: The Evolution of Their Use and Strategies for Their Production

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 936
Author(s):  
Vincenzo D’Amelia ◽  
Teresa Docimo ◽  
Christoph Crocoll ◽  
Maria Manuela Rigano
Keyword(s):  
Large Scale ◽  
Molecular Farming ◽  
Well Being ◽  
Bioactive Molecules ◽  
Scale Production ◽  
The Novel ◽  
Novel Studies ◽  
Industrial Sectors ◽  
Large Scale Production ◽  
Novel Applications

Plants naturally produce a terrific diversity of molecules, which we exploit for promoting our overall well-being. Plants are also green factories. Indeed, they may be exploited to biosynthesize bioactive molecules, proteins, carbohydrates and biopolymers for sustainable and large-scale production. These molecules are easily converted into commodities such as pharmaceuticals, antioxidants, food, feed and biofuels for multiple industrial processes. Novel plant biotechnological, genetics and metabolic insights ensure and increase the applicability of plant-derived compounds in several industrial sectors. In particular, synergy between disciplines, including apparently distant ones such as plant physiology, pharmacology, ‘omics sciences, bioinformatics and nanotechnology paves the path to novel applications of the so-called molecular farming. We present an overview of the novel studies recently published regarding these issues in the hope to have brought out all the interesting aspects of these published studies.

scholarly journals A mini review on emerging targets and Approaches for the synthesis of Anti-Viral compounds: In perspective to COVID-19

2021 ◽  
Vol 21 ◽  
Author(s):  
Maheswara Rao Gokada ◽  
Visweswara Rao Pasupuleti ◽  
Hari Babu Bollikolla
Keyword(s):  
Clinical Trials ◽  
Viral Infection ◽  
Large Scale ◽  
Treatment Options ◽  
Scale Production ◽  
Sudden Increase ◽  
The Novel ◽  
Epidemic Disease ◽  
Large Scale Production ◽  
Novel Coronavirus

: The novel Coronavirus disease (COVID-19) is an epidemic disease which appeared at the end of the year 2019 in a sudden increase in number and considered as a pandemic disease caused by a viral infection and it has threatened most countries towards the emergency search for new anti-SARS-COV drugs /vaccines. At present, the number of clinical trials is ongoing worldwide on different drugs i.e. Hydroxychloroquine, Remedisvir, Favipiravir that utilizes various mechanisms of actions. A few world countries are currently processing for clinical trials which may result in a positive manner. Favipiravir (FPV) represents one of the feasible treatment options for COVID-19, if trials result seems positive. Favipiravir will be one among the developed authoritative possible drugs to warrant the benefits to mankind with large-scale production in order to meet the demands by the current pandemic Covid-19 outbreak and the future epidemic outbreaks. In this review, the authors tried to explore key molecules, which will be supportive for scheming COVID-19 research.

I. S. A. Baud. Forms of Production and Women's Labour: Gender Aspects of Industrialisation in India and Mexico. New Delhi: Sage Publications. 1992. 321 pp.Hardbound. Price: Indian Rs 295.00.

1993 ◽  
Vol 32 (1) ◽  
pp. 129-131
Author(s):  
Naureen Talha
Keyword(s):  
Large Scale ◽  
Indian Society ◽  
New Delhi ◽  
Scale Production ◽  
Female Labour ◽  
Self Employment ◽  
Commodity Production ◽  
Large Scale Production ◽  
Mexican Society ◽  
Small Production

The literature on female labour in Third World countries has become quite extensive. India, being comparatively more advanced industrially, and in view of its size and population, presents a pictures of multiplicity of problems which face the female labour market. However, the author has also included Mexico in this analytical study. It is interesting to see the characteristics of developing industrialisation in two different societies: the Indian society, which is conservative, and the Mexican society, which is progressive. In the first chapter of the book, the author explains that he is not concerned with the process of industrialisation and female labour employed at different levels of work, but that he is interested in forms of production and women's employment in large-scale production, petty commodity production, marginal small production, and self-employment in the informal sector. It is only by analysis of these forms that the picture of females having a lower status is understood in its social and political setting.

An Efficient and Improved Process for the Synthesis of Itopride Hydrochloride and Trimethobenzamide Hydrochloride

2018 ◽  
Vol 15 (4) ◽  
pp. 572-575 ◽  
Author(s):  
Ponnusamy Kannan ◽  
Samuel I.D. Presley ◽  
Pallikondaperumal Shanmugasundaram ◽  
Nagapillai Prakash ◽  
Deivanayagam Easwaramoorthy
Keyword(s):  
Large Scale ◽  
Hydroxylamine Hydrochloride ◽  
Scale Production ◽  
One Pot ◽  
Hydrochloride Salt ◽  
Environmental Friendly ◽  
Large Scale Production ◽  
Non Ulcer Dyspepsia ◽  
Itopride Hydrochloride ◽  
Yield And Purity

Aim and Objective: Itopride is a prokinetic agent used for treating conditions like non-ulcer dyspepsia. Itopride is administered as its hydrochloride salt. Trimethobenzamide is used for treating nausea and vomiting and administered as its hydrochloride salt. The aim is to develop a novel and environmental friendly method for large-scale production of itopride and trimethobenzamide. Materials and Methods: Itopride and trimethobenzamide can be prepared from a common intermediate 4- (dimethylaminoethoxy) benzyl amine. The intermediate is prepared from one pot synthesis using Phyrdroxybenzaldehye and zinc dust and further reaction of the intermediate with substituted methoxy benzoic acid along with boric acid and PEG gives itopride and trimethobenzamide. Results: The intermediate 4-(dimethylaminoethoxy) benzylamine is prepared by treating p-hydroxybenzaldehyde and 2-dimethylaminoethyl chloride. The aldehyde formed is treated with hydroxylamine hydrochloride. The intermediate is confirmed by NMR and the purity is analysed by HPLC. Conclusion: Both itopride and trimethobenzamide were successfully synthesized by this method. The developed method is environmental friendly, economical for large-scale production with good yield and purity.

scholarly journals Cyanobacteria—From the Oceans to the Potential Biotechnological and Biomedical Applications

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 241
Author(s):  
Shaden A. M. Khalifa ◽  
Eslam S. Shedid ◽  
Essa M. Saied ◽  
Amir Reza Jassbi ◽  
Fatemeh H. Jamebozorgi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Biomedical Applications ◽  
Large Scale ◽  
Biological Activities ◽  
Scale Production ◽  
Pharmacological Activities ◽  
Large Scale Production ◽  
Marine Products ◽  
Hiv 1 ◽  
Successful Phase

Cyanobacteria are photosynthetic prokaryotic organisms which represent a significant source of novel, bioactive, secondary metabolites, and they are also considered an abundant source of bioactive compounds/drugs, such as dolastatin, cryptophycin 1, curacin toyocamycin, phytoalexin, cyanovirin-N and phycocyanin. Some of these compounds have displayed promising results in successful Phase I, II, III and IV clinical trials. Additionally, the cyanobacterial compounds applied to medical research have demonstrated an exciting future with great potential to be developed into new medicines. Most of these compounds have exhibited strong pharmacological activities, including neurotoxicity, cytotoxicity and antiviral activity against HCMV, HSV-1, HHV-6 and HIV-1, so these metabolites could be promising candidates for COVID-19 treatment. Therefore, the effective large-scale production of natural marine products through synthesis is important for resolving the existing issues associated with chemical isolation, including small yields, and may be necessary to better investigate their biological activities. Herein, we highlight the total synthesized and stereochemical determinations of the cyanobacterial bioactive compounds. Furthermore, this review primarily focuses on the biotechnological applications of cyanobacteria, including applications as cosmetics, food supplements, and the nanobiotechnological applications of cyanobacterial bioactive compounds in potential medicinal applications for various human diseases are discussed.

scholarly journals A Review on Additive Manufacturing Possibilities for Electrical Machines

Energies ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1940
Author(s):  
Muhammad Usman Naseer ◽  
Ants Kallaste ◽  
Bilal Asad ◽  
Toomas Vaimann ◽  
Anton Rassõlkin
Keyword(s):  
Additive Manufacturing ◽  
Large Scale ◽  
Three Dimensional ◽  
Electrical Machines ◽  
Electromagnetic Properties ◽  
Scale Production ◽  
Performance Parameters ◽  
Large Scale Production ◽  
One Step ◽  
Manufacturing Techniques

This paper presents current research trends and prospects of utilizing additive manufacturing (AM) techniques to manufacture electrical machines. Modern-day machine applications require extraordinary performance parameters such as high power-density, integrated functionalities, improved thermal, mechanical & electromagnetic properties. AM offers a higher degree of design flexibility to achieve these performance parameters, which is impossible to realize through conventional manufacturing techniques. AM has a lot to offer in every aspect of machine fabrication, such that from size/weight reduction to the realization of complex geometric designs. However, some practical limitations of existing AM techniques restrict their utilization in large scale production industry. The introduction of three-dimensional asymmetry in machine design is an aspect that can be exploited most with the prevalent level of research in AM. In order to take one step further towards the enablement of large-scale production of AM-built electrical machines, this paper also discusses some machine types which can best utilize existing developments in the field of AM.

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