Resveratrol regulates body weight in healthy and ovariectomized rats

Previous reports suggest the antiestrogen ICI 182,780 (ICI) does not cross the blood-brain barrier (BBB). However, this hypothesis has never been directly tested. In the present study, we tested whether ICI crosses the BBB, penetrates into brain and hypothalamic tissues, and affects known neuroendocrine functions in ovariectomized rats. Using HPLC with mass spectrometry, ICI (1.0 mg/kg·d, 3 d) was detected in plasma and brain and hypothalamic tissues for up to 24 h with maximum concentrations of 43.1 ng/ml, and 31.6 and 38.8 ng/g, respectively. To evaluate antiestrogenic effects of ICI in the brain after systemic dosing, we tested its ability to block the effect of 17 α-ethinyl estradiol (EE) (0.3 mg/kg, 8 d) on tail-skin temperature abatement in the morphine-dependent model of hot flush and on body weight change. In the morphine-dependent model, EE abated 64% of the naloxone-induced tail-skin temperature increase. ICI pretreatment (1.0, 3.0 mg/kg·d) dose dependently inhibited this effect. ICI (3.0 mg/kg·d) alone showed estrogenic-like actions, abating 30% the naloxone-induced flush. In body weight studies, EE-treated rats weighed 58.5 g less than vehicle-treated rats after 8 d dosing. This effect was partially blocked by ICI (3.0 mg/kg·d) pretreatment. Similar to EE treatment, rats receiving 1.0 or 3.0 mg/kg·d ICI alone showed little weight gain compared with vehicle-treated controls. Thus, ICI crosses the BBB, penetrates into brain and hypothalamic tissues, and has both antiestrogenic and estrogenic-like actions on neuroendocrine-related functions.

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Preliminary phytochemical analysis and the effect of Agave sisalana on body weight and defensive behaviours in ovariectomized rats

Journal of Medicinal Plants Research ◽

10.5897/jmpr2017.6382 ◽

2017 ◽

Vol 11 (34) ◽

pp. 538-548 ◽

Cited By ~ 1

Author(s):

Gonçalves Carneiro Spera de Andrade Telma ◽

Emilia Arlindo da Silva Yara ◽

Gazoni Espinoza Diego ◽

Mateus de Lima Leonardo ◽

Karla de Oliveira Cezar Alessandra ◽

...

Keyword(s):

Body Weight ◽

Ovariectomized Rats ◽

Phytochemical Analysis ◽

Defensive Behaviours ◽

Agave Sisalana

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Lesions of hypothalamic paraventricular nuclei do not prevent the effect of estradiol on energy and fat balance

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1994.267.1.e32 ◽

1994 ◽

Vol 267 (1) ◽

pp. E32-E38 ◽

Cited By ~ 6

Author(s):

A. Dagnault ◽

D. Richard

Keyword(s):

Body Weight ◽

Adrenocorticotropic Hormone ◽

Binding Assay ◽

Placebo Treatment ◽

The Body ◽

Ovariectomized Rats ◽

Paraventricular Nuclei ◽

Chronic Effects ◽

Protein Binding Assay ◽

Hypothalamic Paraventricular Nuclei

The chronic effects of estradiol (E2) on energy balance have been investigated in ovariectomized rats with hypothalamic paraventricular nuclei (PVH) lesions. Body weight and food intake were monitored throughout the E2 treatment, which lasted 26 days. At the end of this treatment, rats were decapitated, and their carcasses were processed to determine the body contents in energy, fat, and protein. Plasma adrenocorticotropic hormone (ACTH) and corticosterone were determined by radioimmunoassay and protein-binding assay at the end of the study. Regardless of whether they were sham- or PVH-lesioned, E2-treated rats ate, expended, and gained significantly less energy than untreated animals. In addition, E2-treated rats deposited less fat and protein than the rats not receiving E2. In contrast to the E2 treatment, PVH lesions accelerated the gains in energy and fat regardless of whether the rats were treated with E2 or with a placebo. There were no interaction effects of PVH lesions and the E2 treatment on energy or fat gains. Plasma levels of corticosterone and ACTH were higher in E2-treated rats than in animals receiving the placebo treatment. The present results provide evidence that the hypothalamic PVH is not an essential neuroanatomical structure in the effects of E2 on energy and fat balances.

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Estrogens and antiestrogens: actions and interactions with fluphenazine on food intake and body weight in rats

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1993.264.6.r1214 ◽

1993 ◽

Vol 264 (6) ◽

pp. R1214-R1218 ◽

Cited By ~ 4

Author(s):

J. M. Gray ◽

S. Schrock ◽

M. Bishop

Keyword(s):

Body Weight ◽

Adipose Tissue ◽

Weight Gain ◽

Food Intake ◽

Ethinyl Estradiol ◽

Body Weight Gain ◽

Fatty Acid Synthetase ◽

Ovariectomized Rats ◽

Synthetase Activity ◽

Concurrent Administration

Treatment of ovariectomized rats for 3 days with 2 micrograms estradiol benzoate (E2B), 6 micrograms ethinyl estradiol, or 1-2 mg of either of the antiestrogens nafoxidine or tamoxifen led to similar decreases in food intake, body weight gain, adipose tissue lipoprotein lipase activity, and hepatic fatty acid synthetase activity, despite their different effects on uterine growth and induction of progestin receptors in pituitary and adipose tissue. Longer-term (2 wk) treatment with tamoxifen resulted in similar transient changes in food intake and body weight gain, as did treatment with E2B. Daily administration of 50 micrograms fluphenazine (FLU) led to significant decreases in body weight, although there was no change in food intake. Concurrent administration of FLU with either E2B or tamoxifen led to additive effects on body weight and food intake change. None of the treatments had any effect on in vitro binding of [3H]tamoxifen to antiestrogen binding sites in pooled hypothalamic-preoptic area samples.

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Relaxin alters the plasma osmolality-arginine vasopressin relationship in the rat

Journal of Endocrinology ◽

10.1677/joe.0.1370505 ◽

1993 ◽

Vol 137 (3) ◽

pp. 505-510 ◽

Cited By ~ 69

Author(s):

R. S. Weisinger ◽

P. Burns ◽

L. W. Eddie ◽

E. M. Wintour

Keyword(s):

Body Weight ◽

Fluid Balance ◽

Arginine Vasopressin ◽

Regression Line ◽

Plasma Osmolality ◽

Normal Pregnancy ◽

Ovariectomized Rats ◽

Log P ◽

Control Groups ◽

Pregnant Rat

ABSTRACT During pregnancy, in women and the rat, there is a resetting of the plasma osmolality–arginine vasopressin relationship (Posmol/PAVP) such that a decrease in Posmol is maintained without suppression of PAVP. This occurs at a time when relaxin is detectable in plasma. The hypothesis tested here was that relaxin could alter the Posmol/PAVP in the non-pregnant rat. One group of ovariectomized rats (n = 15) was treated for 7 days with intravenous synthetic human relaxin (10 μg/h) in 10 pi 0·9% (w/v) NaCl. Controls were two groups of rats either with no treatment (n = 15) or treated with vehicle alone (n = 15). One-third of each group received hypertonic saline (0·4 mol NaCl/l, 2 ml/100 g body weight i.p.) on day 7, and one-third were deprived of water for the final 24 h. All rats were killed by decapitation and blood was collected rapidly (<40 s) for hormone and osmolality assays. The Posmol in all relaxin-treated rats was significantly (P < 0·001) lower than that in both control groups, but the PAVP was unchanged. The log PAVP/Posmol regression line was significantly shifted in elevation (P <0·001) but not in slope. Thus treatment of ovariectomized rats with relaxin caused changes in fluid balance which mimic those occurring in normal pregnancy. Journal of Endocrinology (1993) 137, 505–510

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ROLE OF PROGESTERONE IN THE CONTROL OF SECRETION OF FOLLICLE-STIMULATING HORMONE IN THE IMMATURE FEMALE RAT

Journal of Endocrinology ◽

10.1677/joe.0.0890099 ◽

1981 ◽

Vol 89 (1) ◽

pp. 99-106 ◽

Cited By ~ 3

Author(s):

H. M. A. MEIJS-ROELOFS ◽

P. KRAMER ◽

L. GRIBLING-HEGGE

Keyword(s):

Body Weight ◽

Inhibitory Action ◽

Ovariectomized Rats ◽

Female Rat ◽

Serum Concentrations ◽

Immature Female ◽

Progesterone Treatment ◽

Age Dependent ◽

Intact Rats

The inhibitory action on FSH secretion of combined oestradiol and progesterone treatment of ovariectomized, immature rats was studied at various ages. At all ages studied (13–35 days) an additional inhibitory action of progesterone, if combined with oestradiol, could be found as compared with the effect of oestradiol alone. Until 20 days of age, the rise in serum FSH concentration as measured 2 days after ovariectomy could be completely prevented by administration of 0·05 μg oestradiol/100 g body weight or by administration of a lower dose of oestradiol (0·01–0·025 μg) combined with progesterone (0·5–1·5 mg/100 g body weight). After 20 days neither oestradiol nor the combined oestradiol/progesterone treatment resulted in an FSH concentration similar to that found in intact rats. However, the lowest FSH concentrations were reached by using combinations of oestradiol and progesterone. Using progesterone alone, FSH concentration in ovariectomized rats was significantly reduced between 18 and 30 days of age, but not before or after this period. Taken together with data on uterine weight and serum concentrations of progesterone, these findings suggest that (1) both oestradiol and progesterone exert an age-dependent role in regulating FSH secretion in the immature female rat, and (2) amounts of oestradiol and progesterone capable of maintaining, in ovariectomized rats, uterine weights not different from those in intact rats will maintain near-physiological concentrations of FSH before but not after day 20. Thus, ovarian factors other than oestradiol and progesterone must be involved in the regulation of FSH secretion in the female rat after 20 days of age.

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EFFECTS OF TESTOSTERONE PROPIONATE TREATMENT OF NEONATALLY OVARIECTOMIZED RATS ON GROWTH AND SUBSEQUENT RESPONSIVENESS TO OESTROGEN

Acta Endocrinologica ◽

10.1530/acta.0.0820652 ◽

1976 ◽

Vol 82 (3) ◽

pp. 652-660 ◽

Cited By ~ 1

Author(s):

M. F. Tarttelin ◽

J. E. Shryne ◽

R. A. Gorski

Keyword(s):

Body Weight ◽

Testosterone Propionate ◽

Neonatal Period ◽

Specific Effect ◽

Ovariectomized Rats ◽

Inhibitory Effects ◽

Androgen Treatment ◽

Ovx Rats ◽

Significant Difference ◽

Oestradiol Benzoate

ABSTRACT There was no significant difference in body weight between neonatally ovariectomized (OvX) rats whether given oil treatment or 90 μg testosterone propionate (TP) on day 3, when examined up to 23 weeks of age. When these two animals were injected with oestradiol benzoate (3 μg/day for 2 weeks), the neonatally OvX TP treated rats showed a significantly smaller depression in body weight than did the control neonatally OxX rats. Measurement of food intake also showed that TP treated rats responded significantly less to the depressant effects of oestrogen than did the controls. These data are consistent with the hypothesis that the ovary does restrain body weight in TP rats but that androgen treatment in the neonatal period may not have a specific effect on growth but may alter the sensitivity of growth regulating processes to the inhibitory effects of oestrogen.

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Preventive Effects of Collagen Peptide from Deer Sinew on Bone Loss in Ovariectomized Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/627285 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

He Zhang ◽

Ying Dong ◽

Bin Qi ◽

Li Liu ◽

Guangxin Zhou ◽

...

Keyword(s):

Body Weight ◽

Bone Loss ◽

Female Rats ◽

Ovariectomized Rats ◽

Uterine Weight ◽

Mineral Density ◽

Active Constituent ◽

Collagen Peptide ◽

Calcium Phosphorus ◽

Serum Biochemical

Deer sinew (DS) has been used traditionally for various illnesses, and the major active constituent is collagen. In this study, we assessed the effects of collagen peptide from DS on bone loss in the ovariectomized rats. Wister female rats were randomly divided into six groups as follows: sham-operated (SHAM), ovariectomized control (OVX), OVX given 1.0 mg/kg/week nylestriol (OVX + N), OVX given 0.4 g/kg/day collagen peptide (OVX + H), OVX given 0.2 g/kg/day collagen peptide (OXV + M), and OVX given 0.1 g/kg/day collagen peptide (OXV + L), respectively. After 13 weeks of treatment, the rats were euthanized, and the effects of collagen peptide on body weight, uterine weight, bone mineral density (BMD), serum biochemical indicators, bone histomorphometry, and bone mechanics were observed. The data showed that BMD and concentration of serum hydroxyproline were significantly increased and the levels of serum calcium, phosphorus, and alkaline phosphatase were decreased. Besides, histomorphometric parameters and mechanical indicators were improved. However, collagen peptide of DS has no effect on estradiol level, body weight, and uterine weight. Therefore, these results suggest that the collagen peptide supplementation may also prevent and treat bone loss.

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Neuroprotective effects of dehydroepiandrosterone (DHEA) in rat model of Alzheimer's disease.

Acta Biochimica Polonica ◽

10.18388/abp.2011_2218 ◽

2011 ◽

Vol 58 (4) ◽

Cited By ~ 28

Author(s):

Hanan F Aly ◽

Fateheya M Metwally ◽

Hanaa H Ahmed

Keyword(s):

Oxidative Stress ◽

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Body Weight ◽

Rat Model ◽

Neurotrophic Factor ◽

Intraperitoneal Administration ◽

Brain Derived Neurotrophic Factor ◽

Ovariectomized Rats ◽

Antioxidant Enzyme Activities

The current study was undertaken to elucidate a possible neuroprotective role of dehydroepiandrosterone (DHEA) against the development of Alzheimer's disease in experimental rat model. Alzheimer's disease was produced in young female ovariectomized rats by intraperitoneal administration of AlCl(3) (4.2 mg/kg body weight) daily for 12 weeks. Half of these animals also received orally DHEA (250 mg/kg body weight, three times weekly) for 18 weeks. Control groups of animals received either DHAE alone, or no DHEA, or were not ovariectomized. After such treatment the animals were analyzed for oxidative stress biomarkers such as hydrogen peroxide, nitric oxide and malondialdehyde, total antioxidant capacity, reduced glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase activities, antiapoptotic marker Bcl-2 and brain derived neurotrophic factor. Also brain cholinergic markers (acetylcholinesterase and acetylcholine) were determined. The results revealed significant increase in oxidative stress parameters associated with significant decrease in the antioxidant enzyme activities in Al-intoxicated ovariectomized rats. Significant depletion in brain Bcl-2 and brain-derived neurotrophic factor levels were also detected. Moreover, significant elevations in brain acetylcholinesterase activity accompanied with significant reduction in acetylcholine level were recorded. Significant amelioration in all investigated parameters was detected as a result of treatment of Al-intoxicated ovariectomized rats with DHEA. These results were confirmed by histological examination of brain sections. These results clearly indicate a neuroprotective effect of DHEA against Alzheimer's disease.

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Effect of Collagen Peptide, Alone and in Combination with Calcium Citrate, on Bone Loss in Tail-Suspended Rats

Molecules ◽

10.3390/molecules25040782 ◽

2020 ◽

Vol 25 (4) ◽

pp. 782 ◽

Cited By ~ 1

Author(s):

Junli Liu ◽

Jianing Wang ◽

Yanchuan Guo

Keyword(s):

Body Weight ◽

Bone Loss ◽

Mechanical Tests ◽

Dual Energy ◽

Ovariectomized Rats ◽

Control Group ◽

Calcium Citrate ◽

X Ray ◽

Three Point Bending ◽

Collagen Peptide

Oral administration of bovine collagen peptide (CP) combined with calcium citrate (CC) has been found to inhibit bone loss in ovariectomized rats. However, the protective effects of CP and CP–CC against bone loss have not been investigated in a tail-suspension simulated microgravity (SMG) rat model. Adult Sprague-Dawley rats (n = 40) were randomly divided into five groups (n = 8): a control group with normal gravity, a SMG control group, and three SMG groups that underwent once-daily gastric gavage with CP (750 mg/kg body weight), CC (75 mg/kg body weight) or CP–CC (750 and 75 mg/kg body weight, respectively) for 28 days. After sacrifice, the femurs were analyzed by dual-energy X-ray absorptiometry, three-point bending mechanical tests, microcomputed tomography, and serum bone metabolic markers. Neither CP nor CP–CC treatment significantly inhibited bone loss in SMG rats, as assessed by dual-energy X-ray absorptiometry and three-point bending mechanical tests. However, both CP and CP–CC treatment were associated with partial prevention of the hind limb unloading-induced deterioration of bone microarchitecture, as demonstrated by improvements in trabecular number and trabecular separation. CP–CC treatment increased serum osteocalcin levels. Dietary supplementation with CP or CP–CC may represent an adjunct strategy to reduce the risk of fracture in astronauts.

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