scholarly journals Molecular characterization of cellulolytic (endo- and exoglucanase) bacteria from the largest mangrove forest (Sundarbans), Bangladesh

2020 ◽  
Vol 70 (1) ◽  
Author(s):  
Satyajit Biswas ◽  
Md. Al Saber ◽  
Ismoth Ara Tripty ◽  
Md. Adnan Karim ◽  
Md. Aminul Islam ◽  
...  

Abstract Purpose Cellulase, due to its massive applicability, has been used in various industrial processes such as biofuels (bioethanol, triphasic biomethanation), agricultural and plant waste management, chiral separation, and ligand binding studies. The finding of a novel cellulase-producing bacterium will benefit the industries, which rely on yeast to produce cellulase in fermentation technology, because bacteria can easily be manipulated and fermented cost-effectively. Methods Cellulase enzyme-secreting bacteria were isolated from different regions of the world’s largest mangrove forests, Sundarbans in Bangladesh. Biochemical, morphological, and 16S rRNA identification protocol was followed to precisely characterize the bacterial strains. Result We have determined that the strain T2-D2 (Bacillus sp.), E1-PT (Pseudomonas sp.), and D1-PT (Pseudomonas sp.) showed maximum endoglycolytic and strain C1-BT (Bacillus sp.), E1-BT (Bacillus sp.), and T-4 (E) showed relatively higher exoglycolytic activity during the test. So, it can be easily cultured at a normal temperature (97.7–99.5 °F). On the one hand, T2-D2 (Bacillus sp.) and E1-PT (Pseudomonas sp.) have shown the highest growth rate at pH 7 as it was neither acidic nor basic. Conclusion It was concluded that the strain T2-D2 (Bacillus sp.) and E1-PT (Pseudomonas sp.) would be our target cellulolytic strains wherein the experimental isolates belonged to the Enterobacteriaceae, Psuedomonacea, Bacillacea, and Morganellacea family.

2020 ◽  
Vol 5 (2) ◽  
pp. 66-71
Author(s):  
Moch. Chasan Basri ◽  
Hari Santoso ◽  
Saimul Laili

  Mangrove forests are the one of the coastal ecosystems; especially the northern coast and many are founded in the surrounding fish ecosystems. That organism is the one of the fauna which mangrove ecosystem constituent. The aim of this study was to determine the density and abundance of the population of Gelodok fish at mangrove areas in Penunggul Village, Nguling District. This study used a purposive sampling method from three stations and each station has three plots and three replications in each plot. The results showed that the density of frog fish in the mangrove forest area of ​​Nguling village at station 1 was 3 as well as at station 2 and 3 respectively 2 and 1. At station 1 there were three species, namely Baleopthalmus boddarti, Periopthalmus dipus, Periophthalmus Gracilis, station 2, there were two species; Baleopthalmus boddarti, Periophthalmus Gracilis, and station 3 there were three species namely Baleopthalmus boddarti, Periopthalmodon schlosseri, Periopthalmus dipus. The differences in the results of the study, environmental conditions and abiotic factors influence the density of fish in the mangrove area and at the station 1 the density is highe. Keywords: Gelodok fish, mangrove forest, population ABSTRAK Hutan mangrove merupakan salah satu ekosistem daerah pantai terutama pantai utara dan banyak di temui ikan Gelodok di sekitar ekositem ini. Organisme tersebut merupakan salah satu fauna penyusun ekositem mangrove. Tujuan penelitian ini untuk mengetahui kepadatan dan kemelimpahan populasi ikan Gelodok kawasan mangrove desa Penunggul Kecamatan Nguling. Penelitian ini menggunakan metode purposive sampling dari tiga stasiun dan setiap stasiun terdapat tiga plot dengan ulangan tiga kali dalam setiap plot. Hasil penelitian menunjukkan bahwa kepadatan ikan gelodok di kawasan hutan mangrove desa Nguling rata-rata pada stasiun 1 adalah 3, di satsiun 2; 2 dan 3; 1. Pada stasiun 1 terdapat tiga spesies yaitu Baleopthalmus boddarti, Periopthalmus dipus, Periophthalmus Gracilis , stasiun 2 terdapat dua spesies yaitu Baleopthalmus boddarti, Periophthalmus Gracilis dan stasiun 3 terdapat tiga spesies yaitu Baleopthalmus boddarti, Periopthalmodon schlosseri, Periopthalmus dipus. Perbedaan hasil penelitian, kondisi lingkungan dan faktor abiotik mempengaruhi kepadatan ikan Gelodok di kawasan mangrove, sehingga pada stasiun 1 diperoleh kepadatan  lebih tinggi. Kata kunci: ikan Gelodok, hutan mangrove, kepadatan populasi    


2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.


Author(s):  
Roger R Tabalessy

Coastal areas can either meet the human needs or give great contribution to the development. However, rapid infrastrural development in Sorong, west Papua, has been followed by high demand for mangrove timber and caused mangrove forest degradation due to exploitation. This exploitation could also result from high economic value of the mangrove timber. This study was done to analyze the economic value of mangrove wood utilized by the people to support the development process in Sorong. This study used primary data obtained through interviews and the economic value calculation of mangrove forests. It found that Sorong had mangrove economic value of IDR 165,197,833, 491. Wilayah pesisir selain dapat memenuhi kebutuhan hidup manusia juga memberikan kontribusi yang besar bagi pembangunan. Cepatnya pembangunan infrastruktur di Kota Sorong diikuti pula dengan tingginya permintaan akan kayu mangrove dan menyebabkan terjadinya degradasi hutan mangrove akibat eksploitasi. Eksploitasi ini disebabkan juga akibat kayu mangrove memiliki nilai ekonomi. Penelitian yang dilakukan ini bertujuan untuk menganalisis nilai ekonomi kayu mangrove yang dimanfaatkan oleh masyarakat Kota Sorong dalam proses menunjang pembangunan. Penelitian ini menggunakkan data primer yang diperoleh melalui hasil wawancara dan perhitungan nilai ekonomi hutan mangrove. Hasil penelitian ini menunjukkan nilai ekonomi ekosistem hutan mangrove yang berada di Kota Sorong adalah Rp165.197.833.491.


Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1686
Author(s):  
Andrey Galukhin ◽  
Roman Nosov ◽  
Ilya Nikolaev ◽  
Elena Melnikova ◽  
Daut Islamov ◽  
...  

A new rigid tricyanate ester consisting of seven conjugated aromatic units is synthesized, and its structure is confirmed by X-ray analysis. This ester undergoes thermally stimulated polymerization in a liquid state. Conventional and temperature-modulated differential scanning calorimetry techniques are employed to study the polymerization kinetics. A transition of polymerization from a kinetic- to a diffusion-controlled regime is detected. Kinetic analysis is performed by combining isoconversional and model-based computations. It demonstrates that polymerization in the kinetically controlled regime of the present monomer can be described as a quasi-single-step, auto-catalytic, process. The diffusion contribution is parameterized by the Fournier model. Kinetic analysis is complemented by characterization of thermal properties of the corresponding polymerization product by means of thermogravimetric and thermomechanical analyses. Overall, the obtained experimental results are consistent with our hypothesis about the relation between the rigidity and functionality of the cyanate ester monomer, on the one hand, and its reactivity and glass transition temperature of the corresponding polymer, on the other hand.


Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 473
Author(s):  
Dilyana Gospodonova ◽  
Iliana Ivanova ◽  
Todorka Vladkova

The aim of this study was to prepare TiO2/Ag/Cu magnetron co-sputtered coatings with controlled characteristics and to correlate them with the antimicrobial activity of the coated glass samples. The elemental composition and distribution, surface morphology, wettability, surface energy and its component were estimated as the surface characteristics influencing the bioadhesion. Well expressed, specific, Ag/Cu concentration-dependent antimicrobial activity in vitro was demonstrated toward Gram-negative and Gram-positive standard test bacterial strains both by diffusion 21 assay and by Most Probable Number of surviving cells. Direct contact and eluted silver/coper nanoparticles killing were experimentally demonstrated as a mode of the antimicrobial action of the studied TiO2/Ag/Cu thin composite coatings. It is expected that they would ensure a broad spectrum bactericidal activity during the indwelling of the coated medical devices and for at least 12 h after that, with the supposition that the benefits will be over a longer time.


Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 382
Author(s):  
Camelia-Maria Toma ◽  
Silvia Imre ◽  
Camil-Eugen Vari ◽  
Daniela-Lucia Muntean ◽  
Amelia Tero-Vescan

Plasma protein binding plays a critical role in drug therapy, being a key part in the characterization of any compound. Among other methods, this process is largely studied by ultrafiltration based on its advantages. However, the method also has some limitations that could negatively influence the experimental results. The aim of this study was to underline key aspects regarding the limitations of the ultrafiltration method, and the potential ways to overcome them. The main limitations are given by the non-specific binding of the substances, the effect of the volume ratio obtained, and the need of a rigorous control of the experimental conditions, especially pH and temperature. This review presents a variety of methods that can hypothetically reduce the limitations, and concludes that ultrafiltration remains a reliable method for the study of protein binding. However, the methodology of the study should be carefully chosen.


Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 637
Author(s):  
Huong Thi Thuy Nguyen ◽  
Giles E. S. Hardy ◽  
Tuat Van Le ◽  
Huy Quoc Nguyen ◽  
Hoang Huy Nguyen ◽  
...  

Mangrove forests can ameliorate the impacts of typhoons and storms, but their extent is threatened by coastal development. The northern coast of Vietnam is especially vulnerable as typhoons frequently hit it during the monsoon season. However, temporal change information in mangrove cover distribution in this region is incomplete. Therefore, this study was undertaken to detect change in the spatial distribution of mangroves in Thanh Hoa and Nghe An provinces and identify reasons for the cover change. Landsat satellite images from 1973 to 2020 were analyzed using the NDVI method combined with visual interpretation to detect mangrove area change. Six LULC classes were categorized: mangrove forest, other forests, aquaculture, other land use, mudflat, and water. The mangrove cover in Nghe An province was estimated to be 66.5 ha in 1973 and increased to 323.0 ha in 2020. Mangrove cover in Thanh Hoa province was 366.1 ha in 1973, decreased to 61.7 ha in 1995, and rose to 791.1 ha in 2020. Aquaculture was the main reason for the loss of mangroves in both provinces. Overall, the percentage of mangrove loss from aquaculture was 42.5% for Nghe An province and 60.1% for Thanh Hoa province. Mangrove restoration efforts have contributed significantly to mangrove cover, with more than 1300 ha being planted by 2020. This study reveals that improving mangrove restoration success remains a challenge for these provinces, and further refinement of engineering techniques is needed to improve restoration outcomes.


2020 ◽  
Vol 13 (1) ◽  
pp. 52
Author(s):  
Win Sithu Maung ◽  
Jun Sasaki

In this study, we examined the natural recovery of mangroves in abandoned shrimp ponds located in the Wunbaik Mangrove Forest (WMF) in Myanmar using artificial neural network (ANN) classification and a change detection approach with Sentinel-2 satellite images. In 2020, we conducted various experiments related to mangrove classification by tuning input features and hyper-parameters. The selected ANN model was used with a transfer learning approach to predict the mangrove distribution in 2015. Changes were detected using classification results from 2015 and 2020. Naturally recovering mangroves were identified by extracting the change detection results of three abandoned shrimp ponds selected during field investigation. The proposed method yielded an overall accuracy of 95.98%, a kappa coefficient of 0.92, mangrove and non-mangrove precisions of 0.95 and 0.98, respectively, recalls of 0.96, and F1 scores of 0.96 for the 2020 classification. For the 2015 prediction, transfer learning improved model performance, resulting in an overall accuracy of 97.20%, a kappa coefficient of 0.94, mangrove and non-mangrove precisions of 0.98 and 0.96, respectively, recalls of 0.98 and 0.97, and F1 scores of 0.96. The change detection results showed that mangrove forests in the WMF slightly decreased between 2015 and 2020. Naturally recovering mangroves were detected at approximately 50% of each abandoned site within a short abandonment period. This study demonstrates that the ANN method using Sentinel-2 imagery and topographic and canopy height data can produce reliable results for mangrove classification. The natural recovery of mangroves presents a valuable opportunity for mangrove rehabilitation at human-disturbed sites in the WMF.


2020 ◽  
Vol 70 (6) ◽  
pp. 1275-1288
Author(s):  
Abd El-Mohsen Badawy ◽  
Miroslav Haviar ◽  
Miroslav Ploščica

AbstractThe notion of a congruence pair for principal MS-algebras, simpler than the one given by Beazer for K2-algebras [6], is introduced. It is proved that the congruences of the principal MS-algebras L correspond to the MS-congruence pairs on simpler substructures L°° and D(L) of L that were associated to L in [4].An analogy of a well-known Grätzer’s problem [11: Problem 57] formulated for distributive p-algebras, which asks for a characterization of the congruence lattices in terms of the congruence pairs, is presented here for the principal MS-algebras (Problem 1). Unlike a recent solution to such a problem for the principal p-algebras in [2], it is demonstrated here on the class of principal MS-algebras, that a possible solution to the problem, though not very descriptive, can be simple and elegant.As a step to a more descriptive solution of Problem 1, a special case is then considered when a principal MS-algebra L is a perfect extension of its greatest Stone subalgebra LS. It is shown that this is exactly when de Morgan subalgebra L°° of L is a perfect extension of the Boolean algebra B(L). Two examples illustrating when this special case happens and when it does not are presented.


Sign in / Sign up

Export Citation Format

Share Document