scholarly journals Atopy in Kashmir-validation from a case control study with respect to IgE and Interleukin genes

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Taha Ashraf Qurashi ◽  
Aaliya Shah ◽  
Gulzar Ahmad Bhat ◽  
Mosin Saleem Khan ◽  
Roohi Rasool ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Immunoglobulin E ◽  
Interleukin 4 ◽  
Serum Samples ◽  
Serum Ige ◽  
Genotypic Analysis ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Study Population ◽  
Trained Staff

Abstract Objectives Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) genes with different kind of allergies. Methods A pre-tested questionnaire was used to collect all the dietary, life style and clinical details by a trained staff. A blood sample of 2 ml each was collected in coagulated and anti-coagulated vials. DNA and serum samples were extracted and stored until further use. Serum IgE were estimated by ELISA while as the genotypic analysis was done by PCR–RFLP methods. Results Statistically a significant difference of serum IgE levels were observed among cases and controls (P < 0.05). The observed significant difference of serum IgE levels were retained among subjects who also harboured variant genotypes of IL-4 and IL-13 genes (P < 0.05). Additionally, the above genetic variants significantly modified the risk of allergy when stratification was done based on various clinical characteristics. Conclusion Our study suggests that increased IgE levels and in association with variant forms of IL-4 and IL-13 genes are significantly associated with different types of allergies in study population.

scholarly journals Association of rs780094 polymorphism of glucokinase regulatory protein with non-alcoholic fatty liver disease

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Saba Mohammadi ◽  
Safar Farajnia ◽  
Masoud Shadmand ◽  
Fatemeh Mohseni ◽  
Roghayyeh Baghban
Keyword(s):  
Regulatory Protein ◽  
Single Nucleotide ◽  
Alcoholic Fatty Liver ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Study Population ◽  
Northwest Of Iran ◽  
And Control ◽  
The Relationship ◽  
Tabriz City

Abstract Objective GCK rs780094 polymorphism is a single nucleotide polymorphism that has been associated with obesity, type II diabetes and dyslipidemia in some populations, conditions that highly related to NAFL etiology. The present study aimed to evaluate the relationship between NAFLD and rs780094 polymorphism in patients with NAFLD in Tabriz city, northwest of Iran. The rs780094 polymorphism was determined in 74 patients with NAFLD by PCR–RFLP technique. Demographic information was collected using a questionnaire and biochemical analysis was performed using standard laboratory methods. Results There was a significant difference between case and control subjects for alanine aminotransferase, aspartate aminotransferase, HDL-C and triglycerides (P < 0.05). Analysis by PCR–RFLP method revealed that there were no significant differences between NAFLD and healthy subjects for rs780094 polymorphism in the study population. The results of this study indicated that rs780094 polymorphism is not associated with NAFLD in subjects from Tabriz city.

STAT3 is required for IL-21–induced secretion of IgE from human naive B cells

Blood ◽  
2008 ◽  
Vol 112 (5) ◽  
pp. 1784-1793 ◽  
Author(s):  
Danielle T. Avery ◽  
Cindy S. Ma ◽  
Vanessa L. Bryant ◽  
Brigitte Santner-Nanan ◽  
Ralph Nanan ◽  
...  
Keyword(s):  
B Cells ◽  
Immunoglobulin E ◽  
Interferon Γ ◽  
Fundamental Difference ◽  
Interleukin 4 ◽  
Loss Of Function ◽  
Serum Ige ◽  
Human B Cells ◽  
Stat3 Mutations

Abstract The production of immunoglobulin E (IgE) is tightly regulated. This is evidenced by the fact that it comprises less than 0.0001% of serum Ig, and aberrant production causes atopic conditions, including allergy, rhinitis, and anaphylaxis. Interleukin-4 (IL-4) is a well-characterized inducer of IgE by human and murine B cells, whereas interferon-γ can antagonize this effect. IL-21 has also been recognized for its ability to suppress IL-4–induced IgE production by murine B cells. Here, we identified IL-21 as an inducer of IgE production by CD40L-stimulated human naive B cells. Furthermore, there was a striking synergy between IL-4 and IL-21 on inducing IgE secretion by CD40L-stimulated human B cells, such that the levels detected under these conditions exceeded those induced by IL-4 or IL-21 alone by more than 10-fold. IL-21 induced activation of STAT3 and analysis of B cells from patients with loss-of-function STAT3 mutations revealed that the ability of IL-21 to induce IgE secretion, and augment that driven by IL-4, was STAT3-dependent. These findings highlight a fundamental difference between the regulation of IgE production by human and murine B cells and have implications for the dysregulated production of IgE in conditions characterized by extremely high levels of serum IgE.

scholarly journals Serum vitamin D and immunoglobulin E levels in children of allergic rhinitis: a case-control study from Central India

2021 ◽  
Vol 8 (6) ◽  
pp. 1038
Author(s):  
Payasvi Baweja Sachdeva ◽  
Sheloj Joshi ◽  
Shweta Anand
Keyword(s):  
Vitamin D ◽  
Case Control Study ◽  
Immunoglobulin E ◽  
Serum Vitamin ◽  
Serum Ige ◽  
Serum Vitamin D ◽  
Vitamin D Levels ◽  
Significant Difference ◽  
The Mean ◽  
Control Study

Background: Allergic rhinitis (AR) is the commonest type of non-infective rhinitis. Genetic and environmental factors play an important role in the development of the disease. Researchers are having interest in knowing the role of vitamin D in the pathogenesis of allergy. Immunoglobulin E (IgE) is integral to the pathogenesis of allergic disorders. However, the relationship between serum IgE levels and AR is still a matter of debate. Thus, this study aimed to know serum IgE and serum 25 (OH) vitamin D levels in patients with clinically diagnosed AR and control group.Methods: A case-control study was performed from May 2019 to October 2019 in LNMC and JK hospital. All 54 children with physician diagnosed AR aged 12 to 18 years of both genders who have come in the OPD (total enumeration sampling) during the above mentioned period were enrolled into the study and their 54 healthy counterparts are taken as controls. Other parameters such as age, gender, occupation and region of residence were also compared between the groups.Results: This study found highly significant difference in the mean serum IgE levels while there is a significant difference in the mean serum vitamin D levels between two groups.Conclusions: The study group with AR had significantly higher mean level of serum IgE and lower mean serum vitamin D levels as compared to control groups. However, upon stratification of vitamin D levels, the differences were insignificant. Further studies should be conducted to know the value of IgE as a prognostic factor of AR severity and to throw more light on association of vitamin D with AR.

scholarly journals Some Serum Activity Markers of Airways Inflammation in Difficult-To-Control Asthma Patients

1997 ◽  
Vol 40 (3) ◽  
pp. 61-70
Author(s):  
Bohuslav Král ◽  
Jan Krejsek ◽  
Zdena Paráková ◽  
Otakar Kopecký ◽  
Doris Vokurková ◽  
...  
Keyword(s):  
Cell Activation ◽  
Immunoglobulin E ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Serum Marker ◽  
Interleukin 4 ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Significant Difference ◽  
Control Asthma

The main aim of the present study was a search for a characteristic serum marker of inflammatory activity in the airways of asthmatics with difficult-to-control disease. Therefore, serum levels of interleukin-4 (IL-4), serum low-affinity Fc Epsilon Receptor II (sFcER II), Interferon-gamma (INF-gamma) Immunoglobulin-E (IgE), Interleukin-2 (IL-2), serum Interleukin Receptor 2 (sIL-2R) and Intercellular Adhesion Molecule-1 (sICAM-1) were measured in 2 groups of asthmatics: 1-26 patients with difficult-to-control asthma (DTCA), 2-22 asthmatics, minimally symptomatic (MSA). Results: No significant difference in either measured parameters between the DTCA and MSA group in peripheral blood samples was found. Conclusion: The above mentioned serum markers of T- and B-cell activation as well as the serum ICAM-1 level are not sensitive enough to determine the type, activity and severity of the inflammatory process in the asthmatic airways.

scholarly journals Association of rs780094 polymorphism of glucokinase regulatory protein with non-alcoholic fatty liver disease

2019 ◽  
Author(s):  
Saba Mohammadi ◽  
Safar Farajnia ◽  
Fatemeh Mohseni ◽  
Roghayyeh Baghban ◽  
Masoud Shadmand
Keyword(s):  
Regulatory Protein ◽  
Single Nucleotide ◽  
Alcoholic Fatty Liver ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Study Population ◽  
Northwest Of Iran ◽  
And Control ◽  
The Relationship ◽  
Tabriz City

Abstract Objective GCK rs780094 polymorphism is a single nucleotide polymorphism that has been associated with obesity, type II diabetes and dyslipidemia in some populations, conditions that highly related to NAFL etiology. The present study aimed to evaluate the relationship between NAFLD and rs780094 polymorphism in patients with NAFLD in Tabriz city, northwest of Iran. The rs780094 polymorphism was determined in 74 patients with NAFLD by PCR-RFLP technique. Demographic information was collected using a questionnaire and biochemical analysis was performed using standard laboratory methods.Results There were a significant difference between case and control subjects for alanine aminotransferase, aspartate aminotransferase, HDL-C and triglycerides ( P <0.05). Analysis by PCR-RFLP method revealed that there were no significant differences between NAFLD and healthy subjects for rs780094 polymorphism in the study population. The results of this study indicated that rs780094 polymorphism is not associated with NAFLD in subjects from Tabriz city.

scholarly journals Analysis of Vascular Endothelial Growth Factor (VEGF) -634C/G and +936C/T Polymorphisms and Serum VEGF Levels in Women Suffering From Preeclampsia

2021 ◽  
Author(s):  
Sayed hojjat Hosseini ◽  
Ata Ghadiria ◽  
Abdolah Mousavi Salehi ◽  
Saber Rokhafrooz ◽  
Mahin Najafian ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Control Group ◽  
Vascular Endothelial ◽  
Vegf Gene ◽  
Serum Samples ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Gestational Week ◽  
And Control ◽  
Endothelial Growth Factor

Abstract Preeclampsia (PE) is a syndrome related with pregnancy and characterized by hypertension and proteinuria, occurring in approximately 6-8% of pregnancies and accounting for approximately 40% of premature births. This study aimed to investigate the polymorphisms of -634C/G and +936C/T in VEGF gene and their relationship with serum VEGF levels in pregnant women with PE. In this case-control study, peripheral blood samples were collected from 135 women with PE and 135 normal pregnant women as the control group. DNA was extracted using the phenol-chloroform method. Then, the polymorphisms of VEGF gene were detected by PCR-RFLP method using specific primers. Besides, VEGF concentrations were measured by ELISA method on serum samples and control subjects using ELISA kits. In this research, maternal age, gestational week, maternal hemoglobin and BMI were significantly correlated with the likelihood of PE, while the occurrence season variable was not effective in PE among the pregnant women. There was no significant difference in the two polymorphisms of -634C/G and +936C/T in VEGF gene between the two groups. Also, the serum VEGF level in PE patients was significantly higher than the normal group (P<0.001). Despite a significant increase in serum VEGF concentrations in women with PE, it seems that -634C/G and +936C/T polymorphisms of VEGF gene are not related with the onset of PE. Further studies are required to fully understand the risk factors related to preeclampsia syndrome.

Association of P1635 and P1655 polymorphisms in dysbindin (DTNBP1) gene with schizophrenia

2012 ◽  
Vol 24 (3) ◽  
pp. 155-159 ◽  
Author(s):  
Fatemeh Alizadeh ◽  
Mohammad Amin Tabatabaiefar ◽  
Mohammad Ghadiri ◽  
Mir Saeed Yekaninejad ◽  
Nazanin Jalilian ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Population Studies ◽  
Positive Association ◽  
Multiple Comparisons ◽  
Control Groups ◽  
Significant Positive Association ◽  
Significant Difference ◽  
Pcr Rflp ◽  
And Control ◽  
Epigenetic Processes

Objectives: Schizophrenia (SCZ) is a severe psychiatric disorder with a lifetime prevalence of approximately 1% in most of the populations studied. SCZ is multifactorial with the contribution of multiple susceptibility genes that could act in conjunction with epigenetic processes and environmental factors. There is some evidence supporting the association between genetic variants in dysbindin (DTNBP1) gene and SCZ in populations. In this study, we investigated the association between polymorphisms P1635 and P1655 in dysbindin gene with SCZ.Methods: Totally, 115 unrelated patients with SCZ and 117 unrelated healthy volunteers were studied. Genomic DNA was extracted from blood. Genotyping was done with the PCR-RFLP method. The allele and genotype associations were analysed with X2 test. The Benjamini-Hochberg procedure was used to correct p values for multiple comparisons.Results: The results showed no significant difference between patients and controls in allelic frequencies or genotypic distributions of SNP P1635 (p = 0.809), but a significant difference between the case and control groups for SNP P1655 (p = 0.009) was found. We could also find a significant positive association between A-C haplotype and SCZ (OR = 1.7, 95% CI 1.18–2.42; p = 0.004, pc = 0.02) and a protective effect for A-G haplotype (p = 0.003, OR = 0.57, 95% CI 1.18–2.42; p = 0.003, pc = 0.02).Conclusion: This study may provide further support for the association between SNP polymorphisms in DTNBP1 and SCZ in the Iranian population. Studies with more markers and subjects for various populations will be necessary to understand the genetic contribution of the gene to the development of SCZ.

scholarly journals The Seroepidemiology of Toxoplasmosis in the Lower Fraser Valley of British Columbia

1994 ◽  
Vol 5 (5) ◽  
pp. 218-223 ◽  
Author(s):  
Eileen M Proctor ◽  
Satyendra N Banerjee
Keyword(s):  
British Columbia ◽  
Ethnic Communities ◽  
Childbearing Age ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Unpasteurized Milk ◽  
Significant Difference ◽  
Lower Fraser Valley ◽  
Study Population ◽  
Fraser Valley

Objective: To determine the seroprevalence of toxoplasmosis in vegetarian and nonvegetarian members of different ethnic communities in the lower Fraser Valley of British Columbia.Design: Serum samples were collected from 2027 participants drawn from various ethnic groups and tested byelisafor the presence of immunoglobulin (Ig) G and IgM antibodies toToxoplasma gondii.Coded questionnaires requesting information relevant to the study were completed by each participant. The study population comprised 1334 females and 693 males; ages ranged from 17 to 102 years.Main Results: Four hundred and nineteen (20.7%) individuals were IgG positive with titres ranging from 1:100 to 1:3200. IgM antibodies were detected in only four individuals. The seroprevalence rose with increase in age but there was no significant difference between males and females. A positive correlation was shown between ingestion of meat and between consumption of unpasteurized milk and antibodies toT gondii.Eighty per cent of females between the ages of 17 and 40, of all ethnic origins, were seronegative. Seropositivity did not differ between cat owners and non-cat owners.Conclusions: Women of childbearing age are at risk of acquiring toxoplasmosis during pregnancy and of transmitting the infection transplacentally. Consumption of undercooked meat and unpasteurized milk may result in the acquisition of toxoplasmosis. Data suggest that acquisition of toxoplasmosis is more likely via environmental oocysts or cysts in food source animals than by direct contact with cats.

scholarly journals Relationship of Cord Blood Immunoglobulin E and Maternal Immunoglobulin E with Birth Order and Maternal History of Allergy in Albanian Mother/Neonate Pairs

2017 ◽  
Vol 5 (6) ◽  
pp. 751-756
Author(s):  
Hatixhe Latifi-Pupovci ◽  
Violeta Lokaj-Berisha ◽  
Besa Lumezi
Keyword(s):  
Cord Blood ◽  
Birth Order ◽  
Immunoglobulin E ◽  
Blood Levels ◽  
Significant Difference ◽  
History Of ◽  
Study Population ◽  
The Mean ◽  
Relationship Of ◽  
The Relationship

BACKGROUND: Previous studies reported that familial factors such as birth order and mothers atopy might influence cord blood levels and development of allergies.AIM: The aim of the study was to evaluate the relationship of cord blood IgE and maternal IgE with birth order and mothers history of allergy in Albanian mother/neonate pairs.MATERIAL AND METHODS: Study population represented 291 mother-infant pairs. Mothers were interviewed with a questionnaire for personal history of allergy and pregnancy history whereas serum IgE levels were determined using sandwich IRMA assay.RESULTS: The mean level of cIgE in neonates with detectable levels was 1.59 (n = 78). No significant difference in means of cIgE was found between first born and later born neonates (p = 0.232) and between neonates of mothers with a negative and positive history of allergy (p = 0.125). Also, no significant difference was found between means of mIgE by birth order, whereas there was a significant difference of mIgE between mothers with and without a history of allergy (p = 0.01). In a group of neonates with detectable cIgE levels, maternal IgE levels were moderately correlated with cIgE levels.CONCLUSION: Cord blood IgE is not affected by birth order and mothers history of allergy, whereas mothers IgE are affected by the history of allergy but not by birth order.

Sign in / Sign up

Export Citation Format

Share Document