scholarly journals Evidence of colistin resistance genes (mcr-1 and mcr-2) in wild birds and its public health implication in Egypt

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Zeinab S. Ahmed ◽  
Esraa A. Elshafiee ◽  
Hanan S. Khalefa ◽  
Mona Kadry ◽  
Dalia A. Hamza
Keyword(s):  
Public Health ◽  
Surface Water ◽  
Wild Bird ◽  
Water Sources ◽  
Wild Birds ◽  
Resistant Bacteria ◽  
Potential Hazard ◽  
Isolation And Identification ◽  
E Coli ◽  
The Impact

Abstract Background Antimicrobial resistance has become one of the most severe global threats to human and veterinary Medicine. colistin is an effective therapeutic agent against multi-drug-resistant pathogens. However, the discovery of transferable plasmids that confer resistance to colistin (mcr-1) has led to challenges in medical science. This study describes the role of wild birds in the harbouring and environmental spread of colistin-resistant bacteria, which could pose a potential hazard to human and animal health. Methods In total, 140 faecal samples from wild birds (migratory and resident birds) were tested. Twenty surface water samples were collected from the area in which wild bird trapping was conducted, and 50 human stool samples were collected from individuals residing near the surface water sources and farm buildings. Isolation and identification of Enterobacteriaceae and Pseudomonas aeruginosa from the different samples were performed using conventional culture techniques and biochemical identification. PCR amplification of the mcr genes was performed in all positive isolates. Sequencing of mcr-1 genes from three randomly selected E. coli carrying mcr-1 isolates; wild birds, water and humans was performed. Result The bacteriological examination of the samples showing isolates of Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and P. aeruginosa. The results of multiplex PCR of the mcr genes revealed that E. coli was the most prevalent gram-negative bacterium harbouring the mcr genes, whereas a low prevalence was observed for K. pneumoniae. The prevalence of mcr-1 in resident birds, migratory birds, water sources and humans were 10.4, 20,16.6 and 9.6% while the prevalence of mcr-2 were 1.4, 3.6, 11.1 and 9.6%, respectively. Sequencing of the mcr-1 gene from the three E. coli carrying mcr-1 isolates indicated a possible correlation between the wild bird and surface water isolates. Conclusion The detection of mcr-1-positive bacteria in wild birds in Egypt indicates the possible environmental dissemination of this gene through bird activity. The impact of the interaction between domestic and wild animals on public health cannot be overlooked.

scholarly journals Genetic Investigation of Beta-Lactam Associated Antibiotic Resistance Among Escherichia Coli Strains Isolated from Water Sources

2017 ◽  
Vol 11 (1) ◽  
pp. 203-210 ◽  
Author(s):  
Reza Ranjbar ◽  
Mehrdad Sami
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Surface Water ◽  
Water Resources ◽  
Water Sources ◽  
Resistant Bacteria ◽  
Beta Lactam ◽  
E Coli ◽  
Surface Water Resources ◽  
Extended Spectrum Beta Lactamases

Background: Antimicrobial resistance is an important factor threatening human health. It is widely accepted that antibiotic resistant bacteria such as Escherichia coli (E. coli) released from humans and animals into the water sources, can introduce their resistance genes into the natural bacterial community. Objective: The aim of this study was to investigate the prevalence of blaTEM, blaCTX, blaSHV, blaOXA and blaVEB associated-antibiotic resistance among E. coli bacteria isolated from different water resources in Iran. Methods: The study contained all E. coli strains segregated from different surface water sources. The Kirby-Bauer method and combined discs method was determined in this study for testing antimicrobial susceptibility and strains that produced Extended-Spectrum Beta Lactamases (ESBL), respectively. DNA extraction kit was applied for genomic and plasmid DNA derivation. Finally the frequency of resistant genes including blaTEM, blaCTX, blaSHV, blaOXA and blaVEB in ESBL producing isolates were studied by PCR. Results: One hundred E. coli strains were isolated and entered in the study. The highest antibiotic resistance was observed on clindamycin (96%). Moreover, 38.5% isolates were ESBL producers. The frequency of different ESBLs genes were 37%, 27%, 27%, and 25% for blaTEM, blaCTX, blaSHV, and blaOXA, respectively. The blaVEB wasn’t found in any isolates. Conclusion: The study revealed a high prevalence of CTX-M, TEM, SHV and OXA genes among E. coli strains in surface water resources. In conclusion, these results raised a concern regarding the presence and distribution of these threatening factors in surface water sources and its subsequent outcomes.

scholarly journals Patterns of Antimicrobial Resistance Observed in Escherichia coli Isolates Obtained from Domestic- and Wild-Animal Fecal Samples, Human Septage, and Surface Water

2005 ◽  
Vol 71 (3) ◽  
pp. 1394-1404 ◽  
Author(s):  
Raida S. Sayah ◽  
John B. Kaneene ◽  
Yvette Johnson ◽  
RoseAnn Miller
Keyword(s):  
Escherichia Coli ◽  
Surface Water ◽  
Antimicrobial Resistance ◽  
Antimicrobial Agent ◽  
Companion Animals ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Isolation And Identification ◽  
Fecal Samples ◽  
E Coli

ABSTRACT A repeated cross-sectional study was conducted to determine the patterns of antimicrobial resistance in 1,286 Escherichia coli strains isolated from human septage, wildlife, domestic animals, farm environments, and surface water in the Red Cedar watershed in Michigan. Isolation and identification of E. coli were done by using enrichment media, selective media, and biochemical tests. Antimicrobial susceptibility testing by the disk diffusion method was conducted for neomycin, gentamicin, streptomycin, chloramphenicol, ofloxacin, trimethoprim-sulfamethoxazole, tetracycline, ampicillin, nalidixic acid, nitrofurantoin, cephalothin, and sulfisoxazole. Resistance to at least one antimicrobial agent was demonstrated in isolates from livestock, companion animals, human septage, wildlife, and surface water. In general, E. coli isolates from domestic species showed resistance to the largest number of antimicrobial agents compared to isolates from human septage, wildlife, and surface water. The agents to which resistance was demonstrated most frequently were tetracycline, cephalothin, sulfisoxazole, and streptomycin. There were similarities in the patterns of resistance in fecal samples and farm environment samples by animal, and the levels of cephalothin-resistant isolates were higher in farm environment samples than in fecal samples. Multidrug resistance was seen in a variety of sources, and the highest levels of multidrug-resistant E. coli were observed for swine fecal samples. The fact that water sample isolates were resistant only to cephalothin may suggest that the resistance patterns for farm environment samples may be more representative of the risk of contamination of surface waters with antimicrobial agent-resistant bacteria.

scholarly journals Prevalence of Multidrug-ResistantEscherichia coliIsolated from Drinking Water Sources

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Stephen T. Odonkor ◽  
Kennedy K. Addo
Keyword(s):  
Antimicrobial Agents ◽  
Microbiological Quality ◽  
Multidrug Resistant ◽  
Poor Quality ◽  
Water Sources ◽  
Resistant Bacteria ◽  
Human Consumption ◽  
Isolation And Identification ◽  
E Coli ◽  
Risk Of Death

The control of infectious diseases is badly endangered by the rise in the number of microorganisms that are resistant to antimicrobial agents. This is because infections caused by resistant microorganisms often fail to respond to conventional treatment, resulting in prolonged illness and greater risk of death. Antimicrobial-resistant bacteria are also present in various water sources. This study therefore sought to document the microbiological quality and antibiograms of bacterial isolates (E. colistrains) from six different water sources in order to determine their safety for human consumption and to provide updated antibiotic data for pragmatic treatment of patients. Bacteria isolation and identification was done using API and conventional methods. Antibiotic susceptibility testing was conducted using the Kirby–Bauer method. Results obtained indicated that all the water sources tested were of poor quality. Bacteria isolated includedE. coli,Enterobacterspp.,Klebsiellaspp.,Salmonella typhi,Streptococcusspp.,Proteus vulgaris,Vibrio cholera,Shigellaspp.,Pseudomonas aeruginosa, andEnterococcus faecalis. The prevalence of multidrug-resistantE. coliwas 49.48%.E. coliisolates showed high resistance patterns to the tested antibiotics. They were most resistant to penicillin (32.99%), cefuroxime (28.87%), erythromycin (23.71%), and tetracycline (21.45%). In contrast, they were susceptible to nitrofurantoin (93.8%), cefotaxime and amikacin (91.75%), gentamicin (90.7%), nalidixic acid (89.65%), ciprofloxacin (74.2%), chloramphenicol (69.07%), pipemidic acid (65.97%), and cefuroxime (52.58%). Sixty-three percent (63%) of the multidrug-resistantE. colistrains recorded a multiple antibiotic resistance (MAR) index value >0.2. The susceptible antibiotics, especially the nitrofurantoin, are hence recommended in the practical treatment of waterborne bacterial diseases.

scholarly journals Prevalence and molecular characteristics of multi-resistant Escherichia coli in wild birds

2018 ◽  
Vol 87 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Lina Merkeviciene ◽  
Irena Klimiene ◽  
Rita Siugzdiniene ◽  
Marius Virgailis ◽  
Raimundas Mockeliunas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Species ◽  
Animal Health ◽  
Wild Birds ◽  
Resistant Bacteria ◽  
Molecular Characteristics ◽  
Potential Hazard ◽  
E Coli ◽  
Two Samples ◽  
Mute Swans

Humans and animals share the same bacterial species including the resistant ones. For that reason, epidemiological studies in domestic and wild animals should be performed on a regular basis. Wild, particularly migratory birds, should be investigated as potential carriers of antimicrobial resistant bacteria that can be spread globally in a short time. The aim of this study was to investigate the prevalence and to characterize multi-resistant Escherichia coli in wild birds. Three hundred and ninety two samples were obtained from different bird species including gulls (Larus spp.), mallards (Anas platyrhynchos), mute swans (Cygnus olor), as well as other species of birds. Phenotypical and genotypical resistance of E. coli was investigated. In total 60 isolates from 179 tested were resistant to three or more antimicrobial classes and treated as multi-resistant (33.5%; 95% CI 21.56–45.44); the isolates were obtained from gulls, mallards, swans, and rooks. All of the strains demonstrated resistance to aztreonam and cefpodoxime. The most frequent resistance prevalence of the above-mentioned isolates in vitro was also demonstrated to ampicillin (82%), ampicillin/sulbactam (68%), cefazolin (66%), ceftriaxone (55%), and ciprofloxacin (47%). All E. coli isolates were susceptible to amikacin. The results of polymerase chain reaction confirmed the presence of the genes encoding resistance to beta-lactams, aminoglycosides, tetracycline, amphenicols, trimethoprim, and sulphonamides. Consequently, wild birds might constitute a potential hazard to human and animal health by transmitting multi-resistant E. coli strains to waterways and other environmental sources via bird faeces.

scholarly journals Isolation and Characterization of NDM-Positive Escherichia coli from Municipal Wastewater in Jeddah, Saudi Arabia

2016 ◽  
Vol 60 (9) ◽  
pp. 5223-5231 ◽  
Author(s):  
David Mantilla-Calderon ◽  
Muhammad Raihan Jumat ◽  
Tiannyu Wang ◽  
Pugalenthi Ganesan ◽  
Nada Al-Jassim ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Saudi Arabia ◽  
Antibiotic Resistance ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Potential Hazard ◽  
Content Type ◽  
E Coli ◽  
Untreated Wastewater

ABSTRACTThe emergence of resistance to last-resort antibiotics is a public health concern of global scale. Besides direct person-to-person propagation, environmental pathways might contribute to the dissemination of antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Here, we describe the incidence ofblaNDM-1, a gene conferring resistance to carbapenems, in the wastewater of the city of Jeddah, Saudi Arabia, over a 1-year period.blaNDM-1was detected at concentrations ranging from 104to 105copies/m3of untreated wastewater during the entire monitoring period. These results indicate the ubiquity and high incidence ofblaNDM-1in the local wastewater. To track the bacteria carryingblaNDM-1, we isolatedEscherichia coliPI7, a strain of sequence type 101 (ST101), from wastewater around the Hajj event in October 2013. Genome sequencing of this strain revealed an extensive repertoire of ARGs as well as virulence and invasive traits. These traits were further confirmed by antibiotic resistance profiling andin vitrocell internalization in HeLa cell cultures. Given that this strain remains viable even after a certain duration in the sewerage, and that Jeddah lacks a robust sanitary infrastructure to fully capture all generated sewage, the presence of this bacterium in the untreated wastewater represents a potential hazard to the local public health. To the best of our knowledge, this is the first report of ablaNDM-1-positiveE. colistrain isolated from a nonnosocomial environment in Saudi Arabia and may set a priority concern for the need to establish improved surveillance for carbapenem-resistantE. coliin the country and nearby regions.

scholarly journals Microbiological quality of commercially available poultry feeds sold in Bangladesh

2017 ◽  
Vol 3 (1) ◽  
pp. 52-60
Author(s):  
Nigar Sultana ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Mst Deloara Begum ◽  
...  
Keyword(s):  
Public Health ◽  
Methylene Blue ◽  
Culture Media ◽  
Microbiological Quality ◽  
Control Measures ◽  
Nutrient Agar ◽  
Poultry Feed ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli

The study was conducted aiming at the isolation and identification of pathogens from poultry feed manufactured by four different brands namely A (broiler starter), B (broiler finisher), C (layer starter) and D (layer finisher) sold in retail stores of Rangpur city of Bangladesh. All these samples were collected from four randomly chosen outlets and analyzed by culturing in different culture media such as Nutrient broth (NB), Nutrient agar (NA), Salmonella-Shigella (SS) agar, Eosin methylene blue (EMB) agar, MacConkey agar, Triple sugar iron (TSI) agar slant, Motility, Indole, Urease (MIU) and Saboraud Dextrose agar (SDA) media. The bacterial agents were isolated and examined under light microscope for their gross morphological and conventional biochemical characteristics. The bacteriological analyses were done at the Microbiology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period of January to June, 2014. Total bacterial colonies of all the samples were counted separately according to the American Public Health Association, using nutrient agar medium for total viable count (TVC), Eosine methylene blue (EMB) agar media for total E. coli count (TEC) and Salmonella-Shigella agar for TSC (total salmonella count). Saboraud Dextrose agar (SDA) media was used for detection of fungus. The virulence effect of the organism present in feed were observed by inoculating the organism in poultry. Recorded result showed that average TVC of feed sample A, B, C and D were 5.45x106, 3.28x105, 5.14x106 and 4.53x105 CFU/gm (colony forming unit per gram) respectively. TEC of feed sample A, B, C and D were recorded 6.25x105, 8.26x103, 5.52x105 and 5.65x104 CFU/gm respectively. TSC of feed sample A, B, C and D were recorded 3.15x104, 2.68x103, 4.46x103 and 1.19x104 CFU/gm respectively. The highest TVC, TEC and TSC were found in broiler starter (feed sample A) and lowest TVC, TEC and TSC were found in broiler finisher (feed sample B). Fungal count was 1.85x105 CFU/ gm in layer finisher (feed sample D) could be as a result of their high pathogenecity as reported by researchers elsewhere. These organisms can cause several poultry and farm animal infections specially mycotoxicosis having public health significance to both human and poultry. The presence of high numbers of E. coli and Salmonella spp. in poultry feed were indicative of poor hygienic practices during manufacture, post process contamination and unsatisfactory transportation and reservation. Therefore reinforce the need for preventive control measures, hygienic handling and processing of feeds to reduce the risk of potential human health hazards.Asian J. Med. Biol. Res. March 2017, 3(1): 52-60

scholarly journals Acquisition of Resistant Bowel Flora during a Double-Blind Randomized Clinical Trial of Ertapenem versus Piperacillin-Tazobactam Therapy for Intraabdominal Infections

2005 ◽  
Vol 49 (8) ◽  
pp. 3217-3221 ◽  
Author(s):  
Mark J. DiNubile ◽  
Joseph W. Chow ◽  
Vilas Satishchandran ◽  
Adam Polis ◽  
Mary R. Motyl ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Study Drug ◽  
Resistant Bacteria ◽  
Double Blind ◽  
E Coli ◽  
Double Blind Clinical Trial ◽  
Vancomycin Resistant ◽  
The Impact ◽  
Intraabdominal Infections ◽  
Study Therapy

ABSTRACT Bowel colonization with resistant bacteria can develop in patients receiving broad-spectrum antimicrobial therapy. We compared the impact of two antimicrobial regimens often used to treat intraabdominal infections on susceptibility patterns of bowel flora at the end of therapy. In a double-blind clinical trial, adults with complicated intraabdominal infection requiring surgery were randomized to receive piperacillin-tazobactam (3.375 g every 6 h) or ertapenem (1 g once a day) for 4 to 14 days. Rectal swabs were obtained at baseline and at the end of study therapy to determine the acquisition rates of Enterobacteriaceae resistant to the study drug, extended-spectrum β-lactamase (ESBL)-producing Escherichia coli or Klebsiella species, Pseudomonas aeruginosa resistant to imipenem or piperacillin-tazobactam, and vancomycin-resistant Enterococcus faecalis or Enterococcus faecium. Treated patients were assessable for the acquisition of resistant bacteria if appropriate specimens were obtained at both time points. Enterobacteriaceae resistant to the treatment received were acquired during study therapy by 8/122 assessable piperacillin-tazobactam recipients (6.6%) compared to 0/122 assessable ertapenem recipients (P = 0.007). Neither ESBL-producing E. coli or Klebsiella species nor P. aeruginosa resistant to piperacillin-tazobactam was isolated from patients in either treatment group. Imipenem-resistant P. aeruginosa was acquired by two of the ertapenem recipients (1.6%) versus zero of the piperacillin-tazobactam recipients (P = 0.50). Vancomycin-resistant enterococci were acquired during therapy by 8/125 assessable ertapenem recipients (6.4%) versus 2/123 assessable piperacillin-tazobactam recipients (1.6%; P = 0.10). In this study, the acquisition of resistant Enterobacteriaceae occurred significantly more often in patients treated with piperacillin-tazobactam than in those treated with ertapenem.

scholarly journals Antimicrobial Resistance Pilot Surveillance of Pigs and Chickens in Vietnam, 2017–2019

2021 ◽  
Vol 8 ◽  
Author(s):  
C. V. Tuat ◽  
P. T. Hue ◽  
N. T. P. Loan ◽  
N. T. Thuy ◽  
L. T. Hue ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Surveillance Program ◽  
E Coli ◽  
The North ◽  
Disk Diffusion Assay ◽  
Almost All ◽  
The Impact

Antimicrobial use (AMU) and antimicrobial resistance (AMR) are a growing public health and economic threat in Vietnam. We conducted a pilot surveillance programme in five provinces of Vietnam, two in the south and three in the north, to identify antimicrobial resistance (AMR) in rectal swab samples from pigs and fecal samples from chickens at slaughter points during three different points in time from 2017 to 2019. Escherichia coli (E. coli) and non-typhoidal Salmonella (NTS) isolates were tested for antimicrobial susceptibility using disk diffusion assay for 19 antimicrobial agents belonging to nine antimicrobial classes and Etest for colistin (polymyxin). Almost all E. coli (99%; 1029/1042) and NTS (96%; 208/216) isolates were resistant to at least one antimicrobial agent; 94% (981/1042) of E. coli and 89% (193/216) of NTS isolates were multidrug-resistant (MDR). Higher proportions of E. coli and NTS isolated from chickens were resistant to all antimicrobial classes than those isolates from pigs. There was a significantly higher proportion of MDR NTS isolates from the southern provinces of Ho Chi Minh City and Long An (p = 0.008). Although there were increasing trends of NTS in proportion of resistance to fluoroquinolone over the three surveillance rounds, there was a significant decreasing trend of NTS in proportion of resistance to polymyxin (p = 0.002). It is important to establish an annual AMR surveillance program for livestock in Vietnam to assess the impact of interventions, observe trends and drive decision making that ultimately contributes to reducing AMR public health threat.

scholarly journals The impact of anthropogenic and environmental factors on the variability of Escherichia coli in rivers in southwest Bangladesh

2021 ◽  
Author(s):  
M. M. Majedul Islam ◽  
Md. Atikul Islam
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Surface Water ◽  
Environmental Factors ◽  
Physicochemical Parameters ◽  
Sewage Treatment Plant ◽  
Oxygen Demand ◽  
Faecal Contamination ◽  
E Coli ◽  
The Impact

Abstract Faecal contamination of surface water sources is an important water quality issue worldwide. Although quite a few studies exist on surface water faecal contamination and variability of indicator bacteria, most of the studies have been based on larger river basins and in temperate region. The variability is relatively unknown in local scale and in tropical developing countries. In this study we assess how anthropogenic and environmental factors affect faecal contamination and physicochemical parameters in Rupsha and Bhairab rivers around Khulna city, Bangladesh. Water samples were collected from six locations of the rivers during a wet and dry period in 2018 to measure Escherichia coli (E. coli) concentrations. Water physicochemical parameters—temperature, turbidity, pH, dissolved oxygen, biochemical oxygen demand and chemical oxygen demand were also measured. Higher concentrations of E. coli were found in the sampling sites located near the densely populated urban area compared to the downstream site, which receives fewer amounts of discharges from sewer drains. All the E. coli samples violated bathing water quality standards. E. coli concentrations were found to be correlated positively with precipitation and turbidity. A linear regression model was applied, that explains large part of the variation in E. coli concentration (R2 = 0.42). Water quality index assessment was also ranked the water quality as ‘poor’ category; indicate that the water is unsuitable for uses in domestic and recreational purposes and high health risks involved with the water use. The study findings highlight the problem of untreated sewage discharge into the rivers. Implementation of sewage treatment plant with adequate capacity is highly recommended.

scholarly journals Detection of Antibiotic-Resistant Bacteria, Resistance Determinants, and Mobile Elements in Surface Waters in Lebanon

2021 ◽  
Author(s):  
Jennifer Moussa ◽  
Edmond Abboud ◽  
Sima Tokajian
Keyword(s):  
Surface Water ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Population Mobility ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Resistance Determinants ◽  
Bacterial Contaminants ◽  
Plasmid Analysis

AbstractThe prevalence of antibiotic-resistant bacteria in surface water in Lebanon is a growing concern and understanding the mechanisms of the spread of resistance determinants is essential. We aimed at studying the occurrence of resistant organisms and determinants in surface water sources in Lebanon and understanding their mobilization and transmission. Water samples were collected from five major rivers in Lebanon. 91 isolates were recovered out of which 25 were multidrug-resistant (MDR) and accordingly were further characterized. Escherichia coli and Klebsiella pneumoniae were the most commonly identified MDR isolates. Conjugation assays coupled with in silico plasmid analysis were performed and validated using PCR-based replicon typing (PBRT) to identify and confirm incompatibility groups and the localization of β-lactamase encoding genes. E. coli EC23 carried a blaNDM-5 gene on a conjugative, multireplicon plasmid, while blaCTX-M-15 and blaTEM-1B were detected in the majority of the MDR isolates. Different ST types were identified including the highly virulent E. coli ST131. Our results showed a common occurrence of bacterial contaminants in surface water and an increase in the risk for the dissemination of resistance determinants exacerbated with the ongoing intensified population mobility in Lebanon and the widespread lack of wastewater treatment.

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