scholarly journals Enhancement of the antibacterial potential of plantaricin by incorporation into silver nanoparticles

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sara Adel Amer ◽  
Hala Mohamed Abushady ◽  
Rasha Mohamed Refay ◽  
Mahmoud Ahmed Mailam
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Foodborne Pathogens ◽  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Stability Period ◽  
Genes Encoding ◽  
Pharmaceutical Industries ◽  
Antibacterial Potential

Abstract Background Bacteriocins are proteinaceous compounds produced from lactic acid bacteria. Bacteriocins are well-known for their antibacterial potential and safety for application in food. However, the commercial availability of bacteriocin is facing several limitations; among them is the low yield and short stability period. That calls for a new strategy for overcoming these hurdles. Among these approaches is incorporating bacteriocin in nanoparticles. So, the aim of this study was to enhance the plantaricin produced from isolated Lactobacillus plantarum strain using nanotechnology. Results In this study, the plnEF genes encoding plantaricin EF have been identified and sequenced (accession number of MN172264.1). The extracted bacteriocin (EX-PL) was obtained by the ammonium sulfate method. Then, it was used for biosynthesizing plantaricin-incorporated silver nanoparticles (PL-SNPs). The synthesized nanoparticles were confirmed by SEM-EDAX analysis. The antibacterial activity of both combined (PL-SNPs) and extracted plantaricin (EX-PL) were tested against some strains of foodborne pathogenic bacteria. The results revealed that the antibacterial activities were increased by 99.2% on the combination of bacteriocin with the silver nanoparticle. The MIC of EX-PL (7.6 mg/mL) has been lowered after incorporating into silver nanoparticles and reached 0.004 mg/mL for PL-SNPs. Despite that extracted plantaricin showed no inhibitory activity towards Listeria monocytogenes, plantaricin-incorporated silver nanoparticles displayed inhibitory activity against this strain. Furthermore, the stability period at 4 °C was increased from 5 days to 60 days for EX-PL and PL-SNPs, respectively. Conclusions Plantaricin-incorporated silver nanoparticles possess higher antibacterial activity and more stability than the free one, which makes it more fitting for combating foodborne pathogens and open more fields for applications in both food and pharmaceutical industries. Graphical abstract

Diversity and antibacterial activity of actinomycetes from wetland soil

2010 ◽  
Vol 28 (1) ◽  
pp. 52 ◽  
Author(s):  
Maya George ◽  
Gisha George ◽  
A. A. Mohamed Hatha
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Enzyme Production ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Wetland Soil ◽  
Production Potential ◽  
Soil Actinomycetes ◽  
Test Organisms ◽  
Antibacterial Potential

Diversity of soil actinomycetes isolated from various regions of a wetland was analysed. The strains were assigned under 11 genera according to their morphological and biochemical characteristics. They are: Nocardia, Pseudonocardia, Streptomyces, Micromonospora, Rhodococcus, Actinosynnema, Nocardiodes, Kitasatospora, Gordona, Intrasporangium and Streptoalloteichus. Around 55% of the identified strains are Nocardioform actinomycetes. Screening for their antibacterial activities revealed that 96% of the isolated strains showed different degrees of inhibition potential against 12 test pathogenic bacteria (including various serotypes of Salmonella, Vibrio cholerae, Bacillus subtilis and Escherichia coli). Of these about 41% of strains showed antagonism towards Bacillus subtilis. The Nocardioform actinomycetes exhibited antibacterial activity against 9 out of 12 test organisms. The least antibacterial potential was exhibited by Gordona, Rhodococcus, Micromonospora and Kitasatospora. The isolated strains differed among themselves in their ability to decompose proteins and amino acids and also in enzyme production potential.

Antibacterial potential of essential oils of Zataria multiflora and Mentha piperita, micro- and nano-formulated forms

2020 ◽  
Author(s):  
Mahmoud Osanloo ◽  
Abbas Abdollahi ◽  
Alireza Valizadeh ◽  
Niloufar Abedinpour
Keyword(s):  
Particle Size ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Mentha Piperita ◽  
Zataria Multiflora ◽  
Microdilution Method ◽  
Better Than ◽  
Antibacterial Potential

Background and Objectives: Plant-derived essential oils (EOs) shave many usages in health and medicine, such as anti- bacterial agents. The aim of this study was the improvement of antibacterial activities of two EOs using nanotechnology. Materials and Methods: Antibacterial activity was investigated on four important human pathogenic bacteria using the 96-well plate microdilution method, a quantitative approach. Eleven formulations were prepared using each of the EOs. Eventually, the best nanoformulation with the smallest particle size and polydispersive indices (PDI and SPAN) was selected using each EO for further investigations. Moreover, two microemulsions with similar ingredients and the same portion in comparison with two selected nanoemulsions were also prepared. Antibacterial activity of each EO was compared with its micro- and nano-emulsions. Results: The antibacterial efficacy of Zataria multiflora EO (ZMEO) was significantly better than Mentha piperita EO (MPEO). Besides, the antibacterial activity of nanoemulsion of ZMEO with a particle size of 129 ± 12 nm was significantly better than no- and micro-formulated forms of ZMEO. Interestingly, the efficiency of MPEO nanoemulsion (160 ± 25 nm) was also significantly better than MPEO and its micro-formulated form. Conclusion: Regardless of the intrinsic antibacterial property of two examined EOs, by formulating to nanoemulsion, their efficiencies were improved. Nanoemulsion of ZMEO introduced as an inexpensive, potent and green antibacterial agent.

scholarly journals Bacterial nanobiotic potential

2020 ◽  
Vol 9 (1) ◽  
pp. 203-211 ◽  
Author(s):  
Shabana Wagi ◽  
Ambreen Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Bacterial Strains ◽  
Extracellular Biosynthesis ◽  
Green Production ◽  
Antibacterial And Antifungal ◽  
Antibacterial Potential

AbstractAntibiotics are the chemicals responsible for killing pathogenic bacteria but inappropriate and extensive use of antibiotics is hazardous causing adverse impact on human health. Excessive use of antibiotics has led to the development of multiple-drug resistant bacteria posing health hazards to mankind. The study of nanoparticles has revolutionized the problem solving concerns regarding fields of agriculture, chemistry and medicine. Nanoparticles are smaller than atomic nuclei offering more surface area and greater reactivity. Bacterial silver nanoparticles (AgNPs) were studied for their antibacterial potential. AgNPs from Bacillus subtilus show the highest antibacterial activity. Nanoparticles exhibiting antibacterial activity can be helpful to reduce the toxic impact of synthetic antibiotics. Present work deals with the green production of silver nanoparticles by exploiting indigenous bacteria. These AgNPs were characterized through Fourier transform infrared spectroscopic (FTIR) analysis, transmission electron microscopy (TEM) and UV spectroscopic analysis and were also evaluated for their antibacterial and antifungal potential. The data suggested the extracellular biosynthesis method to be very effective for the biosynthesis of AgNPs in some bacterial strains. Keeping in view the antibacterial potential of studied AgNPs, the present work suggests green production of nanoparticles which can be effectively utilized as environment friendly antibacterial and antifungal agents.

Antibacterial Activities of Ramalin, Usnic Acid and its Three Derivatives Isolated from the Antarctic Lichen Ramalina terebrata

2010 ◽  
Vol 65 (1-2) ◽  
pp. 34-38 ◽  
Author(s):  
Babita Paudel ◽  
Hari Datta Bhattarai ◽  
Hong Kum Lee ◽  
Hyuncheol Oh ◽  
Hyun Woung Shin ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Usnic Acid ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antibacterial Compounds ◽  
The Antarctic ◽  
Antibacterial Potential

The development of new antibacterial compounds is an urgent issue to meet the evolution of resistivity of pathogenic bacteria against the available drugs. The objective of this study was to investigate the antibacterial compounds from the Antarctic lichen species Ramalina terebrata. A total of fi ve compounds, usnic acid, usimine A, usimine B, usimine C, and ramalin, were isolated by bioactivity guided-fractionation of the methanol extract of R. terebrata after several chromatographic procedures. The qualitative antibacterial activities of the crude extract and isolated compounds were determined by the disk diffusion method while the minimum inhibitory concentration (MIC) determination assay gave the quantitative strength of the test samples. All the test samples showed antibacterial activity against Bacillus subtilis. The crude extract and usnic acid showed antibacterial activity against Staphylococcus aureus. The MIC values of the isolated compounds against B. subtilis were in the range of 1 to 26 μg/mL. These observed experimental data showed the strong antibacterial potential of these compounds against B. subtilis.

Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Food Systems ◽  
Antibacterial Activities ◽  
Culture Collection ◽  
Bornyl Acetate ◽  
Bacterial Strains ◽  
Disk Diffusion Assay ◽  
Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

scholarly journals Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 299
Author(s):  
Reetika Singh ◽  
Christophe Hano ◽  
Gopal Nath ◽  
Bechan Sharma
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Room Temperature ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Effect Of Temperature ◽  
Human Pathogenic Bacteria ◽  
Carissa Carandas ◽  
Antioxidant And Antimicrobial Activity

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRESH AND FREEZE-DRIED SELECTED FRUIT JUICES

2017 ◽  
Vol 10 (9) ◽  
pp. 156 ◽  
Author(s):  
Naritsara Toobpeng ◽  
Pannapa Powthong ◽  
Pattra Suntornthiticharoen
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Total Phenolic ◽  
Zone Of Inhibition ◽  
Schleichera Oleosa ◽  
Freeze Dried

  Objective: The objective of this investigation was to assess the biological activity of fresh fruit juices and freeze dried fruit juices including antioxidant and antibacterial activity.Methods: The fruits used in this study were Schleichera oleosa (Lour.) Oken, Carissa carandas L., and Sandoricum koetjape (Burm.f.) Merr. The fresh juice sample (FJS) was extracted and also prepared for freeze drying sample (FDS) by freeze dryer. The antioxidant capacity of FJS and FDS was assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays and determination for total phenolic contents (TPCs) by Folin–Ciocalteu’s reagent. Antimicrobial tests were carried out by agar diffusion methods and evaluated by measuring the zone of inhibition against 10 isolates of pathogenic bacteria.Results: The highest antioxidant activity of FJS and FDS was found in S. oleosa and related to the scavenging effect on DPPH radical of FJS (100% concentration) and FDS (200 mg/ml) was found in the value of 93.05±0.31% and 82.18±0.33%, respectively. TPC of FJS and FDS from S. oleosa at the same concentration as in the DPPH assay was 1,003.53±1.96 ug GAE/300 g of matter and 827.77±23.15 ug GAE/100 ml of matter, respectively. Both FJS and FDS revealed antibacterial activity. The zone of inhibition of FJS was ranging from 8.3±0.6 to 24.0±1.0 mm and FDS values of 7.3±0.5-21.0±0.9 mm. The most potent sample against bacteria was FJS and FDS from S. oleosa which FJSs against Escherichia coli ESBL and FDS against Pseudomonas aeruginosa MDR2.Conclusion: Fruit juices of S. oleosa, C. carandas, and S. koetjape associated with antioxidant activity in the form of both fresh fruit juices and FDS. The result of this study showed that the value of DPPH and TPCs were slightly decreased in FDS compare with fresh juice. Screening of FJS and FDS of C. carandas revealed broad spectrum antibacterial activity. However, FJS of S. oleosa and S. koetjape showed inhibition of growth of bacteria, but few of FDS from these fruits could inhibit limited bacterial isolates. For antibacterial activity against pathogenic bacteria, the samples of fresh and freeze dried exhibited a different degree of activity.

scholarly journals Fabrication of Antimicrobial Perspiration Pads and Cotton Cloth UsingAmaranthus dubiusMediated Silver Nanoparticles

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
M. Jannathul Firdhouse ◽  
P. Lalitha
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Medical Devices ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Antibacterial Agents ◽  
Cotton Cloth ◽  
Textile Materials ◽  
Textile Fabrics ◽  
Antibacterial Textile

Silver nanoparticles prepared through a simplistic method using the aqueous extract ofAmaranthus dubiuswere fabricated on perspiration pads and cotton cloth samples to obtain antibacterial textile materials by two different fabrication methods. The antibacterial activity was investigated against the bacteriaCorynebacteriumwhich is commonly present in sweat. Silver nanoparticles that serve as antibacterial agents, against pathogenic bacteria, have gained increased applications in medical devices, textile fabrics, and food industry and hence the result of this study would be a welcomed option.

Inhibition of Food-Related Pathogenic Bacteria by god-Transformed Penicillium nalgiovense Strains

1999 ◽  
Vol 62 (8) ◽  
pp. 940-943 ◽  
Author(s):  
ROLF GEISEN
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Type Strain ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Wild Type Strain ◽  
Antibacterial Effect ◽  
Wild Type ◽  
Penicillium Nalgiovense ◽  
And Staphylococcus Aureus

Two strains of Penicillium nalgiovense, which carried the god gene of Aspergillus niger and had increased glucose oxidase (GOD) activity compared with the wild-type strain, were tested for their ability to suppress the growth of certain food-related pathogenic bacteria. In contrast to the wild type, which showed no antibacterial effect when grown in mixed culture with different bacteria, the two tranformed strains were highly antagonistic. The strain that expressed higher amounts of GOD in general had higher inhibitory activity. Both strains showed antibacterial activity against Listeria monocytogenes, Salmonella Enteritidis, and Staphylococcus aureus. The inhibitory activity was dependent on the glucose concentration in the medium. S. aureus was completely inhibited at 1% glucose in the presence of the higher GOD-producing transformant. In contrast, if arabinose was used as a carbon source, no inhibition occurred. If catalase was added to the medium, the inhibitory activity of the transformants was completely inactivated, indicating that the hydrogen peroxide produced was responsible for the antibacterial activity of the transformants.

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