Oxidative modification of proteins of blood plasma in the development of ovarian cancer relapse.
e22002 Background: Comparative analysis of oxidative modification of proteins of blood plasma level in patients with ovarian cancer in a state of remission and during the formation of tumor relapse with various intensity of bloodstream in it. Methods: The research includes 61 patients with ovarian cancer, from whom 11 women were in a state of remission, and 50 had local relapse in the cavity of the lesser pelvis. With the help of ultra-sonic scanner we evaluated the intensity of vascularization and the speed of bloodstream in relapsing tumors. We also studied the level of blood plasma spontaneous metal catalyzed oxidative modification of proteins (OMP), spectrophotometrically registering 2, 4-dinitrophenylhydrazines. Results: In remission there was noted the increase of OMP products level by 74.4% under 530 nm (p<0.05) in blood plasma as compared with the its level in healthy women. During the progress of avascular relapse there was noted the increase of OMP products level by 63.3% under 370 nm (p<0.001) and decrease of OMP products level by 32.2% under 530 nm as compared with the level in remission. In the group with hyper intensive bloodstream in relapse tumor the level of OMP products increased by 37.4% under 370nm (p<0.05) and the level of products decreased by 24% under 530 nm as compared with the group with avascular relapse. In case with metal catalyzed OMP the low level of OMP products under 370 nm (by 18-28% lower than the level of OMP of healthy women, p<0.01), the high level of OMP products under 530 nm (by 24-47% higher than the level of OMP of healthy women, p<0.01) remained, and the level of OMP under 430 nm was increasing as compared with healthy women. Conclusions: The increase of OMP products of neutral character registered under 370 nm, and the decrease of the products of the basic character registered under 530 nm take place during the progress of cancerous process and intensification of the bloodstream in a tumor. The dynamics metal catalyzed OMP products shows the heightened formation of the products of the basic character (430 and 530 nm), being the markers of proteins fragmentation.