fushi tarazu protein expression in the cellular blastoderm of Drosophila detected using a novel imaging technique

Development ◽  
1989 ◽  
Vol 106 (1) ◽  
pp. 95-103 ◽  
Author(s):  
T.L. Karr ◽  
T.B. Kornberg
Keyword(s):  
Transcriptional Regulation ◽  
Protein Expression ◽  
Transcriptional Control ◽  
Imaging Technique ◽  
Drosophila Embryo ◽  
Immunoperoxidase Technique ◽  
Fushi Tarazu ◽  
Post Transcriptional Regulation ◽  
Blastoderm Stage ◽  
Anterior Posterior

The fushi tarazu (ftz) gene is essential for segmentation of the Drosophila embryo. This requirement is reflected at the cellular blastoderm stage of embryogenesis by seven transverse stripes of ftz expression. These stripes correspond to the missing segments of ftz mutant embryos. We describe here novel intermediate patterns of ftz protein expression which were detected in younger embryos by using anti-ftz antibodies and a sensitive fluorescence/immunoperoxidase technique (‘filtered fluorescence imaging’, FFI). Striped patterns of ftz protein evolved continuously, and the different stripes appeared in an ordered sequence, involving both anterior-posterior (A/P) and dorsal-ventral (D/V) progressions. Comparison of these patterns of ftz protein with those of ftz RNA suggests that these novel aspects of the patterning process involve post-transcriptional regulation in addition to the transcriptional control known to be involved in expression of this gene.

scholarly journals Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in Drosophila

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1232
Author(s):  
Maria A. Duk ◽  
Vitaly V. Gursky ◽  
Maria G. Samsonova ◽  
Svetlana Yu. Surkova
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Protein Expression ◽  
Drosophila Embryo ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Wild Type ◽  
Segmentation Gene ◽  
Segmentation Gene Expression ◽  
Post Transcriptional Regulation

Unlike transcriptional regulation, the post-transcriptional mechanisms underlying zygotic segmentation gene expression in early Drosophila embryo have been insufficiently investigated. Condition-specific post-transcriptional regulation plays an important role in the development of many organisms. Our recent study revealed the domain- and genotype-specific differences between mRNA and the protein expression of Drosophila hb, gt, and eve genes in cleavage cycle 14A. Here, we use this dataset and the dynamic mathematical model to recapitulate protein expression from the corresponding mRNA patterns. The condition-specific nonuniformity in parameter values is further interpreted in terms of possible post-transcriptional modifications. For hb expression in wild-type embryos, our results predict the position-specific differences in protein production. The protein synthesis rate parameter is significantly higher in hb anterior domain compared to the posterior domain. The parameter sets describing Gt protein dynamics in wild-type embryos and Kr mutants are genotype-specific. The spatial discrepancy between gt mRNA and protein posterior expression in Kr mutants is well reproduced by the whole axis model, thus rejecting the involvement of post-transcriptional mechanisms. Our models fail to describe the full dynamics of eve expression, presumably due to its complex shape and the variable time delays between mRNA and protein patterns, which likely require a more complex model. Overall, our modeling approach enables the prediction of regulatory scenarios underlying the condition-specific differences between mRNA and protein expression in early embryo.

Interactions between the pair-rule genes runt, hairy, even-skipped and fushi tarazu and the establishment of periodic pattern in the Drosophila embryo

Development ◽  
1988 ◽  
Vol 104 (Supplement) ◽  
pp. 51-60 ◽  
Author(s):  
Philip Ingham ◽  
Peter Gergen
Keyword(s):  
Double Mutant ◽  
Early Embryo ◽  
Drosophila Embryo ◽  
Periodic Pattern ◽  
Fushi Tarazu ◽  
Blastoderm Stage ◽  
Pair Rule

The pair-rule genes of Drosophila play a fundamental role in the generation of periodicity in the early embryo. We have analysed the transcript distributions of runt, hairy, even-skipped and fushi tarazu in single and double mutant ernbryos. The results indicate a complex set of interactions between the genes during the blastoderm stage of embryogenesis.

Lateral inhibition and cell fate during neurogenesis in Drosophila: the interactions between scute, Notch and Delta

Development ◽  
1990 ◽  
Vol 110 (1) ◽  
pp. 733-742 ◽  
Author(s):  
C.V. Cabrera
Keyword(s):  
Transcriptional Regulation ◽  
Protein Expression ◽  
Cell Fate ◽  
Lateral Inhibition ◽  
Early Embryo ◽  
Causal Factors ◽  
Post Transcriptional Regulation

A comparison of the patterns of expression of AS-C (T3) RNA and protein suggests that an important level of regulation occurs post-transcriptionally. First, when the RNA is abundant in the early embryo the protein is barely detectable. Later, the protein starts to accumulate in only a subset of the nuclei of those cells expressing the RNA. Only the cells in the subsets become the neuroblasts. This post-transcriptional regulation is suppressed in embryos mutant for the genes Notch and Delta; where all cells expressing RNA accumulate protein. These findings suggest that deployment of T3 protein expression is one of the causal factors that assigns specific fates to the neuroblasts and, in consequence, a basis for the mechanism of lateral inhibition is proposed.

scholarly journals Regulatory signals in messenger RNA: determinants of nutrient–gene interaction and metabolic compartmentation

1998 ◽  
Vol 80 (4) ◽  
pp. 307-321
Author(s):  
John E. Hesketh ◽  
M. Helena Vasconcelos ◽  
Giovanna Bermano
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Mrna Stability ◽  
Transcriptional Control ◽  
Regulation Of Gene Expression ◽  
Untranslated Regions ◽  
Nutritional Requirements ◽  
Control Of Gene Expression ◽  
Metabolic Compartmentation ◽  
Post Transcriptional Regulation

Nutrition has marked influences on gene expression and an understanding of the interaction between nutrients and gene expression is important in order to provide a basis for determining the nutritional requirements on an individual basis. The effects of nutrition can be exerted at many stages between transcription of the genetic sequence and production of a functional protein. This review focuses on the role of post-transcriptional control, particularly mRNA stability, translation and localization, in the interactions of nutrients with gene expression. The effects of both macronutrients and micronutrients on regulation of gene expression by post-transcriptional mechanisms are presented and the post-transcriptional regulation of specific genes of nutritional relevance (glucose transporters, transferrin, selenoenzymes, metallothionein, lipoproteins) is described in detail. The function of the regulatory signals in the untranslated regions of the mRNA is highlighted in relation to control of mRNA stability, translation and localization and the importance of these mRNA regions to regulation by nutrients is illustrated by reference to specific examples. The localization of mRNA by signals in the untranslated regions and its function in the spatial organization of protein synthesis is described; the potential of such mechanisms to play a key part in nutrient channelling and metabolic compartmentation is discussed. It is concluded that nutrients can influence gene expression through control of the regulatory signals in these untranslated regions and that the post-transcriptional regulation of gene expression by these mechanisms may influence nutritional requirements. It is emphasized that in studies of nutritional control of gene expression it is important not to focus only on regulation through gene promoters but also to consider the possibility of post-transcriptional control.

scholarly journals Post-transcriptional regulation of FUS and EWS protein expression by miR-141 during neural differentiation

2017 ◽  
Vol 26 (14) ◽  
pp. 2732-2746 ◽  
Author(s):  
Francesca Svetoni ◽  
Elisa De Paola ◽  
Piergiorgio La Rosa ◽  
Neri Mercatelli ◽  
Daniela Caporossi ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Protein Expression ◽  
Neural Differentiation ◽  
Post Transcriptional Regulation

scholarly journals Gene Array and Protein Expression Profiles Suggest Post-transcriptional Regulation during CD8+T Cell Differentiation

2003 ◽  
Vol 278 (19) ◽  
pp. 17044-17052 ◽  
Author(s):  
Candace M. Cham ◽  
Hui Xu ◽  
James P. O'Keefe ◽  
Fabiola V. Rivas ◽  
Panayiotis Zagouras ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Cell Differentiation ◽  
T Cell ◽  
Protein Expression ◽  
Expression Profiles ◽  
Gene Array ◽  
Cd8 T Cell ◽  
T Cell Differentiation ◽  
Protein Expression Profiles ◽  
Post Transcriptional Regulation

scholarly journals MicroRNAs and post-transcriptional regulation of skeletal development

2014 ◽  
Vol 52 (3) ◽  
pp. R179-R197 ◽  
Author(s):  
Beatriz Gámez ◽  
Edgardo Rodriguez-Carballo ◽  
Francesc Ventura
Keyword(s):  
Transcriptional Regulation ◽  
Transcriptional Control ◽  
Bone Development ◽  
Skeletal Development ◽  
Bone Diseases ◽  
Therapeutic Approaches ◽  
Microarray Expression Data ◽  
Post Transcriptional Regulation ◽  
Microarray Expression ◽  
Dose Dependent

MicroRNAs (miRNAs) have become integral nodes of post-transcriptional control of genes that confer cellular identity and regulate differentiation. Cell-specific signaling and transcriptional regulation in skeletal biology are extremely dynamic processes that are highly reliant on dose-dependent responses. As such, skeletal cell-determining genes are ideal targets for quantitative regulation by miRNAs. So far, large amounts of evidence have revealed a characteristic temporal miRNA signature in skeletal cell differentiation and confirmed the essential roles that numerous miRNAs play in bone development and homeostasis. In addition, microarray expression data have provided evidence for their role in several skeletal pathologies. Mouse models in which their expression is altered have provided evidence of causal links between miRNAs and bone abnormalities. Thus, a detailed understanding of the function of miRNAs and their tight relationship with bone diseases would constitute a powerful tool for early diagnosis and future therapeutic approaches.

Co- and post-transcriptional regulation of Rbm5 and Rbm10 in mouse cells as evidenced by tissue-specific, developmental and disease-associated variation of splice variant and protein expression levels

Gene ◽  
2016 ◽  
Vol 580 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Bartholomew Ozuemba ◽  
Twinkle J. Masilamani ◽  
Julie J. Loiselle ◽  
Benjamin Koenderink ◽  
Kaitlin A. Vanderbeck ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Protein Expression ◽  
Splice Variant ◽  
Tissue Specific ◽  
Expression Levels ◽  
Mouse Cells ◽  
Post Transcriptional Regulation
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