Development of surface pattern during division in Paramecium. II. Defective spatial control in the mutant kin241

Development ◽  
1992 ◽  
Vol 115 (1) ◽  
pp. 319-335 ◽  
Author(s):  
M. Jerka-Dziadosz ◽  
N. Garreau de Loubresse ◽  
J. Beisson

kin241 is a monogenic nuclear recessive mutation producing highly pleiotropic effects on cell size and shape, generation time, thermosensitivity, nuclear reorganization and cortical organization. We have analyzed the nature of the cortical disorders and their development during division, using various specific antibodies labelling either one of the cortical cytoskeleton components, as was previously done for analysis of cortical pattern formation in the wild type. Several abnormalities in basal body properties were consistently observed, although with a variable frequency: extra microtubules in either the triplets or in the lumen; nucleation of a second kinetodesmal fiber; abnormal orientation of the newly formed basal body with respect to the mother one. The latter effect seems to account for the major observed cortical disorders (reversal, intercalation of supplementary ciliary rows). The second major effect of the mutation concerns the spatiotemporal map of cortical reorganization during division. Excess basal body proliferation occurs and is correlated with modified boundaries of some of the cortical domains identified in the wild type on the basis of their basal body duplication pattern. This is the first mutant described in a ciliate in which both the structure and duplication of basal bodies and the body plan are affected. The data support the conclusion that the mutation does not alter the nature of the morphogenetic signal(s) which pervade the dividing cell, nor the competence of cytoskeletal structures to respond to signalling, but affects the local interpretation of the signals.

Development ◽  
1998 ◽  
Vol 125 (7) ◽  
pp. 1305-1314
Author(s):  
M. Jerka-Dziadosz ◽  
F. Ruiz ◽  
F. Beisson

In Paramecium the development of cell shape and surface pattern during division depends on a precise spatial and temporal pattern of duplication of the ciliary basal bodies which are the organizers of the cortical cytoskeleton. According to their localization, basal bodies will duplicate once, more than once or not all and this duplication is coupled with cell division, as is centrosomal duplication in metazoan cells. We describe here a monogenic nuclear recessive mutation, crochu1 (cro1), resulting in abnormal cell shape and cortical pattern and hypersensitivity to nocodazole. The cytological analysis, by immunofluorescence and electron microscopy, demonstrates that the mutation causes hyper duplication of basal bodies and releases both spatial and temporal control of duplication as basal bodies continue to proliferate in interphase and do so at ectopic locations, beneath the surface and in cortical territories where no duplication occurs in the wild type. However, the abnormal surface organization of cro1 cells does not affect the program of basal body duplication during division. By genetic analysis, no interaction was detected with the sm19 mutation which impairs basal body duplication. In contrast, the cro1 mutation suppresses the nocodazole resistance conferred by nocr1, a mutation in a beta-tubulin gene. This interaction suggests that the primary effect of the mutation bears on microtubule dynamics, whose instability, normally increased during division, would persist throughout the interphase and provide a signal for constitutive basal body duplication.


Development ◽  
1989 ◽  
Vol 105 (2) ◽  
pp. 191-211 ◽  
Author(s):  
F. Iftode ◽  
J. Cohen ◽  
F. Ruiz ◽  
A.T. Rueda ◽  
L. Chen-Shan ◽  
...  

The shape of a Paramecium is determined by the organization of its cortex which constitutes most of the cell cytoskeleton. These structures and networks are organized in relation to the approx. 4000 ciliary basal bodies present at the surface. Each basal body is the centre of a polarized and asymmetrical cortical unit. At the whole-cell level, all units are tandemly arranged in parallel rows and form a defined asymmetrical pattern with dorsoventral and anteroposterior polarities. During division, the cortex is the site of the major morphogenetic processes. In order to analyse how the surface pattern and the shape of the cell are reconstructed at each division, we have used specific immunological and cytological probes to map, in space and time, the reorganization of each of the major cytoskeletal cortical components: basal bodies and microtubules, kinetodesmal fibres, epiplasm and outer lattice. This cytological dissection demonstrates that the surface of the dividing cell is progressively invaded by morphogenetic waves which successively and individually trigger the duplication, assembly or reorganization of each structure and which all spread from the same epicentre (oral apparatus and fission furrow) with the same shape. Furthermore, the response of units to the morphogenetic waves depends on their position on the cell. It thus appears that despite the structural local constraints within units, the development of surface pattern is controlled in an integrated manner by transcellular signals.


1976 ◽  
Vol 27 (2) ◽  
pp. 109-122 ◽  
Author(s):  
Françoise Ruiz ◽  
André Adoutte ◽  
Michèle Rossignol ◽  
Janine Beisson

SUMMARYMutation tam38 of Paramecium tetraurelia is a nuclear recessive mutation with a pleiotropic effect on both trichocyst morphogenesis and nuclear processes. The analysis of the defective nuclear processes (micronuclear and macronuclear divisions, nuclear reorganization at autogamy) shows that these defects result from an abnormal localization of the nuclei. Phenocopies of tam38 abnormalities can be obtained by vinblastine treatment of wild-type cells at late stages of division. Taking into account the similarity between tam38 and a series of other mutations which also prevent trichocyst attachment to the cell surface and disturb nuclear divisions, the following interpretation is proposed: the absence of attached trichocyst induces structural changes in the plasma membrane or in the cortical region which disturb the normal cortical control of the localization of nuclei.


2011 ◽  
Vol 106 (5) ◽  
pp. 2662-2674 ◽  
Author(s):  
T. Kao ◽  
J. S. Shumsky ◽  
E. B. Knudsen ◽  
M. Murray ◽  
K. A. Moxon

Spinal cord transection silences neuronal activity in the deafferented cortex to cutaneous stimulation of the body and untreated animals show no improvement in functional outcome (weight-supported stepping) with time after lesion. However, adult rats spinalized since neonates that receive exercise therapy exhibit greater functional recovery and exhibit more cortical reorganization. This suggests that the change in the somatotopic organization of the cortex may be functionally relevant. To address this issue, we chronically implanted arrays of microwire electrodes into the infragranular layers of the hindlimb somatosensory cortex of adult rats neonatally transected at T8/T9 that received exercise training (spinalized rats) and of normal adult rats. Multiple, single neuron activity was recorded during passive sensory stimulation, when the animals were anesthetized, and during active sensorimotor stimulation during treadmill-induced locomotion when the animal was awake and free to move. Our results demonstrate that cortical neurons recorded from the spinalized rats that received exercise 1) had higher spontaneous firing rates, 2) were more likely to respond to both sensory and sensorimotor stimulations of the forelimbs, and also 3) responded with more spikes per stimulus than those recorded from normal rats, suggesting expansion of the forelimb map into the hindlimb map. During treadmill locomotion the activity of neurons recorded from neonatally spinalized rats was greater during weight-supported steps on the treadmill compared with the neuronal activity during nonweight supported steps. We hypothesize that this increased activity is related to the ability of the animal to take weight supported steps and that, therefore, these changes in cortical organization after spinal cord injury are relevant for functional recovery.


2001 ◽  
Vol 118 (5) ◽  
pp. 481-494 ◽  
Author(s):  
Li-Qun Gu ◽  
Stephen Cheley ◽  
Hagan Bayley

Noncovalent molecular adapters, such as cyclodextrins, act as binding sites for channel blockers when lodged in the lumen of the α-hemolysin (αHL) pore, thereby offering a basis for the detection of a variety of organic molecules with αHL as a sensor element. β-Cyclodextrin (βCD) resides in the wild-type αHL pore for several hundred microseconds. The residence time can be extended to several milliseconds by the manipulation of pH and transmembrane potential. Here, we describe mutant homoheptameric αHL pores that are capable of accommodating βCD for tens of seconds. The mutants were obtained by site-directed mutagenesis at position 113, which is a residue that lies near a constriction in the lumen of the transmembrane β barrel, and fall into two classes. Members of the tight-binding class, M113D, M113N, M113V, M113H, M113F and M113Y, bind βCD ∼104-fold more avidly than the remaining αHL pores, including WT-αHL. The lower Kd values of these mutants are dominated by reduced values of koff. The major effect of the mutations is most likely a remodeling of the binding site for βCD in the vicinity of position 113. In addition, there is a smaller voltage-sensitive component of the binding, which is also affected by the residue at 113 and may result from transport of the neutral βCD molecule by electroosmotic flow. The mutant pores for which the dwell time of βCD is prolonged can serve as improved components for stochastic sensors.


2018 ◽  
Vol 51 ◽  
pp. 177-184
Author(s):  
T. V. Shevchuk

Fur is not only a wonderful decoration of clothing, but also one of the insulating factors. Choosing fur, women are guided not only aesthetic considerations, but also practicality of the product. It has a number of physical and organoleptic parameters and commodity characteristics determined genetically. Fox fur is long with degree of density of 60 units and wear – up to 20 years. Colour, tracery, friability, silkiness, and veil availability or silveriness are determined by animal’s interior. The purpose of our research was to establish breeding and genetic characteristics of foxes of different colour types at cage breeding. The earliest object of farming was a fox. It is spread in Europe, Asia and America in the wild. Typically, a wild fox is red colour, but sometimes you can find dark individuals among them – black and brown (in Europe, Asia and America – Alaska), silver-black (in Canada) and intermediate forms between red and black. A characteristic feature of all foxes is a white tail tip. A fox (Vulpes vulpes, Linnaeus, 1758) belongs to the genus Vulpes of the family Canidae of the order Carnivora. There are 6 species belonging to the genus Vulpes; there are 4 breeds (silver-black, burgundy, pearl and kolikott) and 7 types in the State Register. Recessive mutation foxes by colour can be divided into three groups by analogy with minks. Blue group includes pearl colour types of foxes. Pearl colour is very close to a silver-black phenotypically, but has the weakened tone of black axial hair, so it seems that colour is gray-blue or gray-brown. Foxes divided into two recessive forms: kolikott-brown and burgundy in brown group. A burgundy fox has a bright (red-brown) colour than kolikott. Eyes of kolikott are blue, and burgundy fox has yellow-brown ones. A wild red fox is characterized by red colour of various shades from fiery red to almost gray. There are six main types of colour of a red fox: fiery – reddish red; red – bright red, but without fiery hue; red – light red or reddish-yellow; light – light sand-yellow; red and grey – grey with reddish belt along a spine; grey – grey with a dim red back. Variability of colour of wild foxes is largely associated with habitat. The ears and ends of paws (to a carpal joint on front paws and to rear hock) are black. A tip tail is usually white or grey because of grey fluff or certain parts of pigmented hair. Black hair is quite often on a tail and body. Fluff is various shades of grey or brown all over the body. Albinos are found among foxes as among other animals. They have a pure white colour fluff, depigmented end of nose and claw, light blue eyes with a reddish tint. The colour of white foxes is recessive in relation to the colour of wild foxes. Other names of this fox are Georgian White, Bakuriani. This breed was obtained in Bakuriani fur farm in the forties of the twentieth century. Their coloration is white with black ears and black spots on a face, a back and legs. Creamy shades are considered undesirable. It has been established that homozygotes in this type of colour tend to die. There are two known fox breeds determining colour: silver-black and black-brown. The silver-black fox originates from wild foxes in Canada, black-brown one – in Eurasia and Alaska. Therefore, black-brown foxes are often called Alaskan silver-black in foreign literature. The silver-black and black-brown foxes can differ externally only that the black-brown fox has hair bundle of brown colour, located near inner edge of a base of an auricle. Sometimes significant development of red (different tone and intensity) spots behind ears, on sides, scapulas and at root of the tail is observed in some black-brown foxes. Awn hair with white area in the middle of them is called silver. Feature of fox silveriness is that it can be extended across a back, sides (silver hair can’t be on a belly), on a neck or to grab only some parts of body. For successful breeding of fur-bearing animals in captivity it needs to know their biological characteristics. Keeping of fox under conditions of fur farms began recently. Furry animals are in the earliest stages of domestication, so they have retained many features and physiological properties characterizing animals in the wild. One of the features is nature of nervous activity. Animals of cage keeping have features of wild, so they can’t be picked up without certain warnings; they do not respond to the call of a person, someone of them is evil, while others show timidity. The second feature of furry animals of cage keeping is related to nature of nutrition. The third feature of furry animals is seasonality of their basic life processes – reproduction, moulting, and metabolism. Conclusions. 1. There are three main groups of foxes according to colour: black, blue and brown at cage breeding. 2. Foxes of original red, silver-black, pearl and "ice" colours are perspective among all colour types in selection.


2004 ◽  
Vol 186 (8) ◽  
pp. 2495-2498 ◽  
Author(s):  
John S. Van Arnam ◽  
Jonathan L. McMurry ◽  
May Kihara ◽  
Robert M. Macnab

ABSTRACT Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.


2006 ◽  
Vol 74 (2) ◽  
pp. 1171-1180 ◽  
Author(s):  
Jason W. Johnston ◽  
David E. Briles ◽  
Lisa E. Myers ◽  
Susan K. Hollingshead

ABSTRACT The concentration of Mn2+ is 1,000-fold higher in secretions than it is at internal sites of the body, making it a potential signal by which bacteria can sense a shift from a mucosal environment to a more invasive site. PsaR, a metal-dependent regulator in Streptococcus pneumoniae, was found to negatively affect the transcription of psaBCA, pcpA, rrgA, rrgB, rrgC, srtBCD, and rlrA in the presence of Mn2+. psaBCA encode an ABC-type transporter for Mn2+. pcpA, rrgA, rrgB, and rrgC encode several outer surface proteins. srtBCD encode a cluster of sortase enzymes, and rlrA encodes a transcriptional regulator. Steady-state RNA levels are high under low Mn2+ concentrations in the wild-type strain and are elevated under both high and low Mn2+ concentrations in a psaR mutant strain. RlrA is an activator of rrgA, rrgB, rrgC, and srtBCD (D. Hava and A. Camilli, Mol. Microbiol. 45:1389-1406, 2002), suggesting that PsaR may indirectly control these genes through rlrA, while PsaR-dependent repression of psaBCA, pcpA, and rlrA transcription is direct. The impact of Mn2+-dependent regulation on virulence was further examined in mouse models of pneumonia and nasopharyngeal carriage. The abilities of ΔpsaR, pcpA, and ΔpsaR ΔpcpA mutant strains to colonize the lung were reduced compared to those of the wild type, confirming that both PcpA-mediated gene regulation and PsaR-mediated gene regulation are required for full virulence in the establishment of pneumonia. Neither PcpA nor PsaR was found to be required for colonization of the nasopharynx in a carriage model. This is the first demonstration of Mn2+ acting as a signal for the expression of virulence factors within different host sites.


Author(s):  
Thecan Caesar-Ton That ◽  
Lynn Epstein

Nectria haematococca mating population I (anamorph, Fusarium solani) macroconidia attach to its host (squash) and non-host surfaces prior to germ tube emergence. The macroconidia become adhesive after a brief period of protein synthesis. Recently, Hickman et al. (1989) isolated N. haematococca adhesion-reduced mutants. Using freeze substitution, we compared the development of the macroconidial wall in the wild type in comparison to one of the mutants, LEI.Macroconidia were harvested at 1C, washed by centrifugation, resuspended in a dilute zucchini fruit extract and incubated from 0 - 5 h. During the incubation period, wild type macroconidia attached to uncoated dialysis tubing. Mutant macroconidia did not attach and were collected on poly-L-lysine coated dialysis tubing just prior to freezing. Conidia on the tubing were frozen in liquid propane at 191 - 193C, substituted in acetone with 2% OsO4 and 0.05% uranyl acetate, washed with acetone, and flat-embedded in Epon-Araldite. Using phase contrast microscopy at 1000X, cells without freeze damage were selected, remounted, sectioned and post-stained sequentially with 1% Ba(MnO4)2 2% uranyl acetate and Reynold’s lead citrate. At least 30 cells/treatment were examined.


2020 ◽  
Vol 20 (8) ◽  
pp. 1262-1267
Author(s):  
Haojun Yang ◽  
Hanyang Liu ◽  
YuWen Jiao ◽  
Jun Qian

Background: G protein-coupled bile acid receptor (TGR5) is involved in a number of metabolic diseases. The aim of this study was to identify the role of TGR5 after Roux-en-Y gastric bypass (GBP). Methods: Wild type and TGR5 knockout mice (tgr5-/-) were fed a high-fat diet (HFD) to establish the obesity model. GBP was performed. The changes in body weight and food intake were measured. The levels of TGR5 and peptide YY (PYY) were evaluated by RT-PCR, Western blot, and ELISA. Moreover, the L-cells were separated from wild type and tgr5-/- mice. The levels of PYY in L-cells were evaluated by ELISA. Results: The body weights were significantly decreased after GBP in wild type mice (p<0.05), but not tgr5-/- mice (p>0.05). Food intake was reduced after GBP in wild type mice, but also not significantly affected in tgr5-/- mice (p>0.05). The levels of PYY were significantly increased after GBP compared with the sham group (p<0.05); however, in tgr5-/- mice the expression of PYY was not significantly affected (p>0.05). After INT-777 stimulation in L-cells obtained from murine intestines, the levels of PYY were significantly increased in L-cells tgr5+/+ (p<0.05). Conclusion: Our study suggests that GBP up-regulated the expression of TGR5 in murine intestines, and increased the levels of PYY, which further reduced food intake and decreased the body weight.


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