Different mechanisms initiate and maintain wingless expression in the Drosophila wing hinge

Development ◽  
2002 ◽  
Vol 129 (17) ◽  
pp. 3995-4004
Author(s):  
David del Álamo Rodríguez ◽  
Javier Terriente ◽  
Máximo I. Galindo ◽  
Juan Pablo Couso ◽  
Fernando J. Díaz-Benjumea

The Drosophila gene wingless encodes a secreted signalling molecule that is required for many patterning events in both embryonic and postembryonic development. In the wing wingless is expressed in a complex and dynamic pattern that is controlled by several different mechanisms. These involve the Hedgehog and Notch pathways and the nuclear proteins Pannier and U-shaped. In this report, we analyse the mechanisms that drive wingless expression in the wing hinge. We present evidence that wingless is initially activated by a secreted signal that requires the genes vestigial, rotund and nubbin. Later in development, wingless expression in the wing hinge is maintained by a different mechanism, which involves an autoregulatory loop and requires the genes homothorax and rotund. We discuss the role of wingless in patterning the wing hinge.

Development ◽  
1997 ◽  
Vol 124 (5) ◽  
pp. 1007-1018 ◽  
Author(s):  
J.F. Celis de

The differentiation of the veins in the Drosophila wing involves the coordinate activities of several signal transduction pathways, including those mediated by the transmembrane receptors Torpedo and Notch. In this report, the role of the signalling molecule Decapentaplegic during vein differentiation has been analysed. It is shown that decapentaplegic is expressed in the pupal veins under the control of genes that establish vein territories in the imaginal disc. Decapentaplegic, acting through its receptor Thick veins, activates vein differentiation and restricts expression of both veinlet and the Notch-ligand Delta to the developing veins. Genetic combinations between mutations that increase or reduce Notch, veinlet and decapentaplegic activities suggest that the maintenance of the vein differentiation state during pupal development involves cross-regulatory interactions between these pathways.


2018 ◽  
Author(s):  
Brett Buttliere

Over the last decade, there have been many suggestions to improve how scientists answer their questions, but far fewer attempt to improve the questions scientists are asking in the first place. The goal of the paper is then to examine and summarize synthesize the evidence on how to ask the best questions possible. First is a brief review of the philosophical and empirical literature on how the best science is done, which implicitly but not explicitly mentions the role of psychology and especially cognitive conflict. Then we more closely focus on the psychology of the scientist, finding that they are humans, engaged in a meaning making process, and that cognitive conflict is a necessary input for any learning or change in the system. The scientific method is, of course, a specialized meaning making process. We present evidence for this central role of cognitive conflict in science by examining the most discussed scientific papers between 2013 and 2017, which are, in general, controversial and about big problems (e.g., whether vaccines cause autism, how often doctors kill us with their mistakes). Toward the end we discuss the role of science in society, suggesting science itself is an uncertainty reducing and problem solving enterprise. From this basis we encourage scientists to take riskier stances on bigger topics, for the good of themselves and society generally.


2019 ◽  
Vol 25 (27) ◽  
pp. 2909-2918 ◽  
Author(s):  
Joanna Giemza-Stokłosa ◽  
Md. Asiful Islam ◽  
Przemysław J. Kotyla

Background:: Ferritin is a molecule that plays many roles being the storage for iron, signalling molecule, and modulator of the immune response. Methods:: Different electronic databases were searched in a non-systematic way to find out the literature of interest. Results:: The level of ferritin rises in many inflammatory conditions including autoimmune disorders. However, in four inflammatory diseases (i.e., adult-onset Still’s diseases, macrophage activation syndrome, catastrophic antiphospholipid syndrome, and sepsis), high levels of ferritin are observed suggesting it as a remarkable biomarker and pathological involvement in these diseases. Acting as an acute phase reactant, ferritin is also involved in the cytokine-associated modulator of the immune response as well as a regulator of cytokine synthesis and release which are responsible for the inflammatory storm. Conclusion:: This review article presents updated information on the role of ferritin in inflammatory and autoimmune diseases with an emphasis on hyperferritinaemic syndrome.


Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 663
Author(s):  
Yu Yuan ◽  
Abdalla Adam ◽  
Chen Zhao ◽  
Honglei Chen

Release of immunoreactive negative regulatory factors such as immune checkpoint limits antitumor responses. PD-L1 as a significant immunosuppressive factor has been involved in resistance to therapies such as chemotherapy and target therapy in various cancers. Via interacting with PD-1, PD-L1 can regulate other factors or lead to immune evasion of cancer cells. Besides, immune checkpoint blockade targeting PD-1/PD-L1 has promising therapeutic efficacy in the different tumors, but a significant percentage of patients cannot benefit from this therapy due to primary and acquired resistance during treatment. In this review, we described the utility of PD-L1 expression levels for predicting poor prognosis in some tumors and present evidence for a role of PD-L1 in resistance to therapies through PD-1/PD-L1 pathway and other correlating signaling pathways. Afterwards, we elaborate the key mechanisms underlying resistance to PD-1/PD-L1 blockade in cancer immunotherapy. Furthermore, promising combination of therapeutic strategies for patients resistant to PD-1/PD-L1 blockade therapy or other therapies associated with PD-L1 expression was also summarized.


2008 ◽  
Vol 294 (3) ◽  
pp. H1183-H1187 ◽  
Author(s):  
Kristen M. Park ◽  
Mario Trucillo ◽  
Nicolas Serban ◽  
Richard A. Cohen ◽  
Victoria M. Bolotina

Store-operated channels (SOC) and store-operated Ca2+ entry are known to play a major role in agonist-induced constriction of smooth muscle cells (SMC) in conduit vessels. In microvessels the role of SOC remains uncertain, in as much as voltage-gated L-type Ca2+ (CaL2+) channels are thought to be fully responsible for agonist-induced Ca2+ influx and vasoconstriction. We present evidence that SOC and their activation via a Ca2+-independent phospholipase A2 (iPLA2)-mediated pathway play a crucial role in agonist-induced constriction of cerebral, mesenteric, and carotid arteries. Intracellular Ca2+ in SMC and intraluminal diameter were measured simultaneously in intact pressurized vessels in vitro. We demonstrated that 1) Ca2+ and contractile responses to phenylephrine (PE) in cerebral and carotid arteries were equally abolished by nimodipine (a CaL2+ inhibitor) and 2-aminoethyl diphenylborinate (an inhibitor of SOC), suggesting that SOC and CaL2+ channels may be involved in agonist-induced constriction of cerebral arteries, and 2) functional inhibition of iPLA2β totally inhibited PE-induced Ca2+ influx and constriction in cerebral, mesenteric, and carotid arteries, whereas K+-induced Ca2+ influx and vasoconstriction mediated by CaL2+ channels were not affected. Thus iPLA2-dependent activation of SOC is crucial for agonist-induced Ca2+ influx and vasoconstriction in cerebral, mesenteric, and carotid arteries. We propose that, on PE-induced depletion of Ca2+ stores, nonselective SOC are activated via an iPLA2-dependent pathway and may produce a depolarization of SMC, which could trigger a secondary activation of CaL2+ channels and lead to Ca2+ entry and vasoconstriction.


2014 ◽  
Vol 56 (3) ◽  
pp. 681-713 ◽  
Author(s):  
Katherine Bowie

AbstractDespite a growing literature revealing the presence of millenarian movements in both Theravada and Mahayana Buddhist societies, scholars have been remarkably reluctant to consider the role of messianic beliefs in Buddhist societies. Khruubaa Srivichai (1878–1938) is the most famous monk of northern Thailand and is widely revered as atonbun, or saint. Althoughtonbunhas been depoliticized in the modern context, the term also refers to a savior who is an incarnation of the coming Maitreya Buddha. In 1920 Srivichai was sent under arrest to the capital city of Bangkok to face eight charges. This essay focuses on the charge that he claimed to possess the god Indra's sword. Although this charge has been widely ignored, it was in fact a charge of treason. In this essay, I argue that the treason charge should be understood within the context of Buddhist millenarianism. I note the saint/savior tropes in Srivichai's mytho-biography, describe the prevalence of millenarianism in the region, and detail the political economy of the decade of the 1910s prior to Srivichai's detention. I present evidence to show that the decade was characterized by famine, dislocation, disease, and other disasters of both natural and social causes. Such hardships would have been consistent with apocalyptic omens in the Buddhist repertoire portending the advent of Maitreya. Understanding Srivichai in this millenarian context helps to explain both the hopes of the populace and the fears of the state during that tumultuous decade.


2007 ◽  
Vol 7 (2) ◽  
pp. 119-128 ◽  
Author(s):  
Melissa C. Garcia ◽  
Mohammed Abbasi ◽  
Shaneen Singh ◽  
Qi He

2010 ◽  
Vol 120 (6) ◽  
pp. 219-229 ◽  
Author(s):  
Madhav Lavu ◽  
Shashi Bhushan ◽  
David J. Lefer

H2S (hydrogen sulfide), viewed with dread for more than 300 years, is rapidly becoming a ubiquitously present and physiologically relevant signalling molecule. Knowledge of the production and metabolism of H2S has spurred interest in delineating its functions both in physiology and pathophysiology of disease. Although its role in blood pressure regulation and interaction with NO is controversial, H2S, through its anti-apoptotic, anti-inflammatory and antioxidant effects, has demonstrated significant cardioprotection. As a result, a number of sulfide-donor drugs, including garlic-derived polysulfides, are currently being designed and investigated for the treatment of cardiovascular conditions, specifically myocardial ischaemic disease. However, huge gaps remain in our knowledge about this gasotransmitter. Only by additional studies will we understand more about the role of this intriguing molecule in the treatment of cardiovascular disease.


Development ◽  
1998 ◽  
Vol 125 (15) ◽  
pp. 2951-2962 ◽  
Author(s):  
T. Klein ◽  
A.M. Arias

The Notch signalling pathway plays an important role during the development of the wing primordium, especially of the wing blade and margin. In these processes, the activity of Notch is controlled by the activity of the dorsal specific nuclear protein Apterous, which regulates the expression of the Notch ligand, Serrate, and the Fringe signalling molecule. The other Notch ligand, Delta, also plays a role in the development and patterning of the wing. It has been proposed that Fringe modulates the ability of Serrate and Delta to signal through Notch and thereby restricts Notch signalling to the dorsoventral boundary of the developing wing blade. Here we report the results of experiments aimed at establishing the relationships between Fringe, Serrate and Delta during wing development. We find that Serrate is not required for the initiation of wing development but rather for the expansion and early patterning of the wing primordium. We provide evidence that, at the onset of wing development, Delta is under the control of apterous and might be the Notch ligand in this process. In addition, we find that Fringe function requires Su(H). Our results suggest that Notch signalling during wing development relies on careful balances between positive and dominant negative interactions between Notch ligands, some of which are mediated by Fringe.


2016 ◽  
pp. 79-108 ◽  
Author(s):  
Konrad Matyjaszek

The rules of the reservation. On the book Jewish Poland Revisited by Erica Lehrer The paper offers a review of Erica Lehrer’s Jewish Poland Revisited, a publication presenting outcomes of an anthropological research on Jewish-Polish memory projects in Cracow's former Jewish district of Kazimierz. In a discussion of the book's theses, the author critically analyses Lehrer's postulate of 'ethnography of possibility' and the resultant strategy of approval for contemporary Kazimierz as a 'space of encounter' alongside with its rules of participation, imposed by the Polish proprietors of the district on its visitors.The article focuses on two such rules that condition a visitor’s possibility of participation in shrinking public spaces of Kazimierz. First of these laws is discussed as an imperative of abandoning the immediacy of district's physical space and its histories signified by the surviving built environment. Instead, Lehrer introduces a conceptual division of "social" and "physical" spaces, which leads to silencing of otherwise immediately present evidence of the violent past. The second rule is analyzed as a requirement of accepting the contemporary Polish owners’ role of 'brokers" and "purveyors" of Jewish heritage, consequential with an approval of a doubtful legal and moral title to the appropriated spaces.Through focusing on these rules of participation that determine and perpetuate the conditionality of Jewish presence in the space of Kazimierz, the author argues for a necessity of questioning and re-defining the traditional divisions of disciplines that establish conceptual separations of "social" and "built" spaces, as well as for a necessity of a critical outlook on contemporary Central European understandings of "heritage". Such an inquiry is discussed as conditional for overcoming the largely avoided yet still present "heritages" in the history of Polish-Jewish relations: the traditions of violence and exclusion, either social and spatial. Regulamin rezerwatu. O książce Jewish Poland Revisited Eriki LehrerArtykuł stanowi recenzję książki Jewish Poland Revisited Eriki Lehrer, prezentującej wyniki antropologicznych badań na temat żydowsko-polskich projektów pamięci realizowanych w byłej dzielnicy żydowskiej na krakowskim Kazimierzu. Omawiając tezy pracy, autor poddaje krytycznej analizie proponowany przez Lehrer projekt etnografii możliwości i wynikającą z niego strategię akceptacji współczesnego Kazimierza jako przestrzeni spotkania, za którą idzie akceptacja zasad uczestnictwa narzuconych gościom przez polskich zarządców Kazimierza.W artykule rozpatrywane są dwie takie zasady, warunkujące możliwość uczestnictwa gościa w kurczącej się przestrzeni publicznej Kazimierza. Pierwszą z nich autor opisuje jako nakaz porzucenia bezpośrednio dostępnej, fizycznej przestrzeni dzielnicy i niesionych przez nią historii, których znakiem jest ocalała zabudowa. W to miejsce Lehrer wprowadza podział na przestrzeń społeczną i fizyczną, skutkiem czego stłumione zostają ślady brutalnej przeszłości, w przeciwnym razie bezpośrednio obecne. Drugą zasadę autor odtwarza jako wymóg akceptacji roli współczesnych polskich właścicieli jako brokerów i pośredników żydowskiego dziedzictwa, co w konsekwencji pociąga za sobą akceptację ich wątpliwych prawnie i moralnie roszczeń do zawłaszczonej przestrzeni.Skupienie uwagi na regulaminie uczestnictwa, który ustanawia i utrzymuje warunkowy charakter żydowskiej obecności w przestrzeni Kazimierza, prowadzi autora do wniosku o konieczności rewaluacji i redefinicji tradycyjnego rozdziału dyscyplin, który tworzy konceptualny podział na społeczne przestrzenie i architektoniczne obiekty, oraz do krytycznego namysłu nad obowiązującym obecnie w Europie Środkowo-Wschodniej rozumieniem pojęcia „dziedzictwo”. Tego rodzaju poszukiwanie uznaje autor za warunek przezwyciężenia ignorowanego zwykle, choć mimo wszystko obecnego w polsko-żydowskich stosunkach „dziedzictwa”: tradycji przemocy i wykluczenia, tak społecznego, jak i przestrzennego. 


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