scholarly journals Anti-allergic Effect of Tea-Leaf Saponin (TLS) from Tea Leaves (Camellia sinensis var. sinensis).

1997 ◽  
Vol 20 (5) ◽  
pp. 565-567 ◽  
Author(s):  
Masaaki AKAGI ◽  
Nobuyuki FUKUISHI ◽  
Tomoko KAN ◽  
Yuko M. SAGESAKA ◽  
Reiko AKAGI
Keyword(s):  
Camellia Sinensis ◽  
Tea Leaves ◽  
Tea Leaf ◽  
Allergic Effect

scholarly journals FORMULATION OF LOTION FROM BLACK TEA EXTRACT (CAMELLIA SINENSIS LINNAEUS) AS SUNSCREEN

2019 ◽  
Vol 11 (1) ◽  
pp. 205 ◽  
Author(s):  
Iyan Sopyan ◽  
Riestya Dwi Permata ◽  
Dolih Gozali ◽  
Insan Sunan Kurniawan Syah
Keyword(s):  
Camellia Sinensis ◽  
Black Tea ◽  
Ultraviolet Rays ◽  
Tea Leaves ◽  
Security Testing ◽  
Black Tea Extract ◽  
Emulsifying Agent ◽  
Irritation Test ◽  
Tea Extract ◽  
Tea Leaf

Objective: The goal of the project was to explore extracts from black tea leaves (Camellia sinensis Linnaeus) to prove it can give protection against ultraviolet rays (UV) in lotion preparation.Methods: Black tea extract was made in sunscreen lotion in oil in water (O/W) type emulsion using a combination of PEG-8 and beeswax as emulsifying agent 1, and combination of cetyl alcohol, ceteth-20, and steareth-20 as emulsifying agent 2. Evaluation of lotion including phytochemical screening of black tea leaf extract, measurement of sun protecting factor (SPF) value of extract black tea leaves, measurement of SPF preparation lotion sunscreen extract black tea leaves, physical observation of preparation, qualitative preparation evaluation using thin layer chromatography (TLC) and lotion security testing have been done to ensure the quality of lotions.Results: The result exhibited the effective SPF that was different to the sunscreen lotion F1, which contained 0.03% w/v and F2 which contain of 0.04% w/v black tea leaves extract with the point of SPF is 20.31 and 24.71 respectively. Both formulas fulfilled the requirements as lotion preparations and did not irritate the skin based on an irritation test on 20 volunteers.Conclusion: Formulas F1 and F2 can be applied as a sunscreen with good physical quality and is safe for topical use in lotion preparation.

scholarly journals PENETAPAN KADAR FENOL TOTAL DAN KATEKIN DAUN TEH HITAM DAN EKSTRAK ASETON TEH HITAM DARI TANAMAN CAMELLIA SINENSIS VAR. ASSAMICA

Jurnal Kimia ◽  
2020 ◽  
pp. 43
Author(s):  
N. L. P. V. Paramita ◽  
N. P. T. W. Andari ◽  
N. M. D. Andani ◽  
N. M. P. Susanti
Keyword(s):  
Camellia Sinensis ◽  
Black Tea ◽  
Acetone Extract ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Tea Leaves ◽  
Tea Leaf ◽  
Acetone Extracts

Indonesia is quite well known as one of the countries that has tea plantations and uses its own production of tea. As a tea producing country, Indonesia has been able as a tea exporter. The most tea production in Indonesia is black tea product. Black tea products are produced from species Camellia sinensis Var. Assamica which undergoes an enzymatic oxidation process to fresh tea leaves. The most phenolic compounds contained in the black tea leaf are catechins, theaflavins, and thearubigin. The purpose of this study was to determine the phenolic total and catechin content of black tea products and acetone extracts produced by D'wan Tea (DT) and Bali Cahaya Amertha (BCA) plantations which located in Tabanan district, Bali. Determination of total phenol was carried out on black tea leaf products and acetone extract using the Folin-Ciocalteu method compared with gallic acid standards. Determination of catechin content was performed on fresh (before oxidized) tea leaves, black tea leaf products, and acetone extracts using the UV-Vis Spectrophotometry method at a maximum wavelength of 257 nm. The results showed that total phenol content of D'wan Tea black tea products (SDT) and Bali Cahaya Amerta (SBCA) were 1.50 ± 0.02 % mg GAE/g dan 0.97 ± 0.02 % mg GAE/g, respectively. Results of the total phenol content of D'Awan Tea (EADT) black tea acetone extract was 6.92 ± 0.12 % mg GAE/g, while the Bali Cahaya Amerta (EABCA) black tea acetone extract was 7.38 ± 0.07 % mg GAE/g. Catechin content from fresh leaf samples of D'wan Tea (SSDT), SDT, and EADT were 15.30 ± 0.22 % mg/g, 8.37 ± 0.13 % mg/g, and 37.45 ± 0.74 % mg/g. Catechin content of fresh leaf samples of Bali Cahaya Amerta (SSBCA), SBCA, and EABCA were 14.71 ± 0.28 % mg/g, 15.16 ± 0.58 % mg/g, and 35.66 ± 0.89 % mg/g. Total phenol content of DT black tea products is greater than BCA black tea products. DT black tea products appear to have decreased catechins in processing. In the processing of black tea it is necessary to decrease catechins content due to the formation of catechin polymers, namely theaflavin and thearubigin as a determinant of the quality of black tea.   Keywords: black tea, Camellia Sinensis, cathecin, total phenol.

scholarly journals FORMULASI SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 109-118
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves ◽  
Tea Leaf

Abstract   Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

Elution of Cs and Sr from tea leaves (Camellia sinensis (L.) Kuntze) to green tea evaluated by PIXE analysis

2018 ◽  
Vol 28 (03n04) ◽  
pp. 101-106
Author(s):  
A. Terakawa ◽  
H. Ushijima ◽  
K. Ishii ◽  
M. Sato ◽  
K. Sera
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Internal Standard ◽  
Hot Water ◽  
Internal Standard Method ◽  
Tea Leaves ◽  
Pixe Analysis ◽  
X Ray ◽  
Tea Leaf ◽  
Alkali Elements

We performed a particle-induced X-ray emission (PIXE) analysis of tea leaf samples (Camellia sinensis (L.) Kuntze) to evaluate the possibility of internal radiation exposure due to the intake of green tea contaminated with [Formula: see text]Cs and [Formula: see text]Sr. The tea leaf samples were obtained from tea trees (Camellia sinensis cv. ‘Yabukita’) cultivated with stable Cs and Sr added to the soil. We prepared the used leaf samples which were placed in distilled hot water (90[Formula: see text] for 1 min) as well as the unused leaf samples. The results of PIXE analysis combined with the internal standard method using Indium as a standard element showed little elution of Ca and Sr from the leaves to green tea, while the concentration of alkali elements, including Cs in the leaf, decreased by 30–40% after the tea was extracted.

scholarly journals POTENSI EKSTRAK KASAR ALGA COKELAT (Sargassum sp) DAN DAUN TEH (Camellia sinensis) DALAM MENGHAMBAT OKSIDASI PADA UDANG VANNAMEI (Litopenaeus vannamei) SEGAR SELAMA PENYIMPANAN DINGIN (Potential of Crude Extract of Brown Algae (Sargassum sp) and Tea Leaves (Camellia sinensis) for Inhibits Oxidation of Pasific White Shrimps (Litopenaeus vannamei) During Refrigerated Storage)

2017 ◽  
Vol 13 (1) ◽  
pp. 45
Author(s):  
Suci Kusumastuti Nur Azizah ◽  
Eko Nurcahya Dewi ◽  
A. Suhaeli Fahmi
Keyword(s):  
Phenolic Compounds ◽  
Camellia Sinensis ◽  
Litopenaeus Vannamei ◽  
Brown Algae ◽  
Leaf Extract ◽  
Thiobarbituric Acid ◽  
White Shrimp ◽  
Refrigerated Storage ◽  
Tea Leaves ◽  
Tea Leaf

 Penggunaan antioksidan menggunakan bahan alami bertujuan untuk menghambat oksidasi dan memperpanjang masa simpan. Salah satu potensi rumput laut cokelat dan teh  yaitu mempunyai kandungan fenol dan flavonoid yang bersifat antioksidan. Produk hasil perikanan seperti udang cepat mengalami proses kemunduran mutu karena kandungan air dan proteinnya yang tinggi. Tujuan penelitian ini adalah mengetahui potensi alga cokelat  (Sargassum sp) dan daun teh (Camellia sinensis) dalam menghambat oksidasi pada udang vannamei (Litopenaeus vannamei) selama penyimpanan dingin. Materi yang digunakan dalam penelitian ini adalah alga cokelat, daun teh, dan udang vannamei. Metode penelitian yang digunakan adalah eksperimental menggunakan rancangan acak lengkap (RAL) pola faktorial. variabel yang diamati adalah nilai Angka Peroksida (PV), Thiobarbituric Acid (TBA), Total Volatil Base Nitrogen (TVBN), dan nilai organoleptik. Ekstrak alga cokelat memiliki senyawa fenol dan flavonoid berturut-turut sebesar 1,81% dan 0,102% dengan IC50 sebesar 98,236 ppm. Ekstrak daun teh memiliki senyawa fenol dan flavonoid berturut-turut sebesar 0,867% dan 0,042% dengan IC50 sebesar 122,673 ppm. Hasil uji untuk melihat tingkat oksidasi lemak yaitu Peroksida Value dan Thiobarbituric Acid  menunjukkan ekstrak alga cokelat dan ekstrak daun teh memberikan pengaruh nyata dan dapat menghambat oksidasi pada udang vannamei hingga hari ke – 12. Hasil uji TVBN dan nilai organoleptik menunjukkan ekstrak alga cokelat memberikan pengaruh yang lebih tinggi daripada ekstrak daun teh.  One of the potential of brown seaweed that has phenol and flavonoid compounds that are antioxidants. Tea also contains compounds an antioxidants. The use of antioxidants using natural materials was to prevent oxidation and extend the shelf life. Shrimp highly perishable since the high water and protein contents. The purpose of this research was to determine the potential of brown algae (Sargassum sp) and tea leaves (Camellia sinensis) for inhibiting oxidation of pasific white shrimp (Litopenaeus vannamei) during refrigerated storage. The material that used in this research was a brown algae, tea leaves, and pasific white shrimp. The research method that used was experimental laboratories with the patterns of completely randomized design test with essentially factorial design. Variables value are PV, TBA, TVBN, and organoleptic value. Extracts of brown algae (Sargassum sp) has phenolic compounds and flavonoid compounds were 1.81% and 0.102% with IC50  98.236 ppm. Tea leaf extract (Camellia sinensis) has phenolic compounds and flavonoids, respectively for 0.867% and 0.042% with IC50 of 122.673 ppm. PV value and TBA value results showed brown algae extract and tea leaf extract significant effect and can inhibit the oxidation of pasific white shrimp until  12 days storage. The result of TVBN value and organoleptic value showed brown algae extact gives higher impact than tea leaf extract.  

scholarly journals Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1434
Author(s):  
Hiran A. Ariyawansa ◽  
Ichen Tsai ◽  
Jian-Yuan Wang ◽  
Patchareeya Withee ◽  
Medsaii Tanjira ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Leaf Spot ◽  
Phylogenetic Trees ◽  
Fungal Infections ◽  
Elongation Factor ◽  
Fungal Species ◽  
Tea Leaves ◽  
Phenotypic Characters ◽  
Leaf Spots ◽  
Molecular Phylogenetic

Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan.

Revealing the endophytic mycoflora in tea (Camellia sinensis) leaves in Sri Lanka: the first comprehensive study

Phytotaxa ◽  
2021 ◽  
Vol 514 (3) ◽  
pp. 247-260
Author(s):  
KASUN THAMBUGALA ◽  
DINUSHANI DARANAGAMA ◽  
SAGARIKA KANNANGARA ◽  
THENUKA KODITUWAKKU
Keyword(s):  
Sri Lanka ◽  
Camellia Sinensis ◽  
Endophytic Fungi ◽  
Sequence Data ◽  
Fungal Endophytes ◽  
Fungal Endophyte ◽  
Tea Leaves ◽  
Molecular Sequence Data ◽  
Molecular Sequence ◽  
Rdna Sequence Data

Endophytic fungi are a diverse group of microorganisms that live asymptomatically in healthy tissues of host and they have been reported from all kinds of plant tissues such as leaves, stems, roots, flowers, and fruits. In this study, fungal endophytes associated with tea leaves (Camellia sinensis) were collected from Kandy, Kegalle, and Nuwara Eliya districts in Sri Lanka and were isolated, characterized, and identified. A total of twenty endophytic fungal isolates belonging to five genera were recovered and ITS-rDNA sequence data were used to identify them. All isolated endophytic fungal strains belong to the phylum Ascomycota and the majority of these isolates were identified as Colletotrichum species. Phyllosticta capitalensis was the most commonly found fungal endophyte in tea leaves and was recorded in all three districts where the samples were collected. This is the very first investigation on fungal endophytes associated with C. sinensis in Sri Lanka based on molecular sequence data. In addition, a comprehensive account of known endophytic fungi reported worldwide on Camellia sinensis is provided.

scholarly journals Functional Characterization of An Allene Oxide Synthase Involved in Biosynthesis of Jasmonic Acid and Its Influence on Metabolite Profiles and Ethylene Formation in Tea (Camellia sinensis) Flowers

2018 ◽  
Vol 19 (8) ◽  
pp. 2440 ◽  
Author(s):  
Qiyuan Peng ◽  
Ying Zhou ◽  
Yinyin Liao ◽  
Lanting Zeng ◽  
Xinlan Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Jasmonic Acid ◽  
Camellia Sinensis ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Tea Leaves ◽  
Ethylene Formation ◽  
Horticultural Crops ◽  
Metabolite Profiles ◽  
Oxide Synthase

Jasmonic acid (JA) is reportedly involved in the interaction between insects and the vegetative parts of horticultural crops; less attention has, however, been paid to its involvement in the interaction between insects and the floral parts of horticultural crops. Previously, we investigated the allene oxide synthase 2 (AOS2) gene that was found to be the only JA synthesis gene upregulated in tea (Camellia sinensis) flowers exposed to insect (Thrips hawaiiensis (Morgan)) attacks. In our present study, transient expression analysis in Nicotiana benthamiana plants confirmed that CsAOS2 functioned in JA synthesis and was located in the chloroplast membrane. In contrast to tea leaves, the metabolite profiles of tea flowers were not significantly affected by 10 h JA (2.5 mM) treatment as determined using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, and gas chromatography-mass spectrometry. Moreover, JA treatment did not significantly influence ethylene formation in tea flowers. These results suggest that JA in tea flowers may have different functions from JA in tea leaves and other flowers.

scholarly journals Optimization of HIB Value Combination of Tween 60 and Span 80 on Cream Formulation of Ethanol Extract of Green Tea Leaves (Camellia Sinensis L.)

2018 ◽  
Vol 23 (3) ◽  
pp. 124
Author(s):  
Fransiska Lisa Anindya Putri ◽  
Akhmad Kharis Nugroho ◽  
Erna Prawita Setyowati
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Lattice Design ◽  
Dpph Method ◽  
Green Tea Leaves ◽  
Span 80 ◽  
Tween 60

Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is supported by polyphenol compound catechin. This research aims to determine the optimum Hydrophilic-Lipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2-Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type. The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature (28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60 and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability 18.44±0.06 cm2, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness after being stored for 4 weeks.

Sign in / Sign up

Export Citation Format

Share Document