Sulfatides Inhibit Adhesion, Migration, and Invasion of Murine Melanoma B16F10 Cell Line in Vitro

A subset of long non-coding RNAs (lncRNAs), categorized as miRNA-host gene lncRNAs (lnc-miRHGs), is processed to produce miRNAs and involved in cancer progression. This work aimed to investigate the influences and the molecular mechanisms of lnc-miRHGs MIR497HG in bladder cancer (BCa). The miR-497 and miR-195 were derived from MIR497HG. We identified that lnc-miRHG MIR497HG and two harbored miRNAs, miR-497 and miR-195, were downregulated in BCa by analyzing The Cancer Genome Atlas and our dataset. Silencing of MIR497HG by CRISPR/Cas13d in BCa cell line 5637 promoted cell growth, migration, and invasion in vitro. Conversely, overexpression of MIR497HG suppressed cell progression in BCa cell line T24. MiR-497/miR-195 mimics rescued significantly the oncogenic roles of knockdown of MIR497HG by CRISPR/Cas13d in BCa. Mechanistically, miR-497 and miR-195 co-ordinately suppressed multiple key components in Hippo/Yap and transforming growth factor β signaling and particularly attenuated the interaction between Yap and Smad3. In addition, E2F4 was proven to be critical for silencing MIR497HG transcription in BCa cells. In short, we propose for the first time to reveal the function and mechanisms of MIR497HG in BCa. Blocking the pathological process may be a potential strategy for the treatment of BCa.

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Depletion of MUC5B mucin in gastrointestinal cancer cells alters their tumorigenic properties: implication of the Wnt/β-catenin pathway

Biochemical Journal ◽

10.1042/bcj20170348 ◽

2017 ◽

Vol 474 (22) ◽

pp. 3733-3746 ◽

Cited By ~ 11

Author(s):

Fatima Lahdaoui ◽

Mathieu Messager ◽

Audrey Vincent ◽

Flora Hec ◽

Anne Gandon ◽

...

Keyword(s):

Cancer Cells ◽

Cell Line ◽

Cancer Cell ◽

Gastrointestinal Cancer ◽

Tumour Growth ◽

Cancer Cell Line ◽

Migration And Invasion ◽

Down Regulation

Secreted mucins are large O-glycosylated proteins that participate in the protection/defence of underlying mucosae in normal adults. Alteration of their expression is a hallmark of numerous epithelial cancers and has often been correlated to bad prognosis of the tumour. The secreted mucin MUC5B is overexpressed in certain subtypes of gastric and intestinal cancers, but the consequences of this altered expression on the cancer cell behaviour are not known. To investigate the role of MUC5B in carcinogenesis, its expression was knocked-down in the human gastric cancer cell line KATO-III and in the colonic cancer cell line LS174T by using transient and stable approaches. Consequences of MUC5B knocking-down on cancer cells were studied with respect to in vitro proliferation, migration and invasion, and in vivo on tumour growth using a mouse subcutaneous xenograft model. Western blotting, luciferase assay and qRT–PCR were used to identify proteins and signalling pathways involved. In vitro MUC5B down-regulation leads to a decrease in proliferation, migration and invasion properties in both cell lines. Molecular mechanisms involved the alteration of β-catenin expression, localization and activity and decreased expression of several of its target genes. In vivo xenografts of MUC5B-deficient cells induced a decrease in tumour growth when compared with MUC5B-expressing Mock cells. Altogether, the present study shows that down-regulation of MUC5B profoundly alters proliferation, migration and invasion of human gastrointestinal cancer cells and that these alterations may be, in part, mediated by the Wnt/β-catenin pathway emphasizing the potential of MUC5B as an actor of gastrointestinal carcinogenesis.

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Lobaplatin arrests cell cycle progression, induces apoptosis and impairs migration and invasion in B16-F10 melanoma cell line in vitro

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2014.12.011 ◽

2015 ◽

Vol 69 ◽

pp. 402-408 ◽

Cited By ~ 17

Author(s):

Fangfang Yang ◽

Yang Yu ◽

Qian Lei ◽

Anqi Zeng ◽

Yali Li ◽

...

Keyword(s):

Cell Cycle ◽

Cell Line ◽

Melanoma Cell ◽

Melanoma Cell Line ◽

Cell Cycle Progression ◽

Migration And Invasion ◽

Cycle Progression

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<i>In-Vitro</i> Effect of Sex Steroids on Mouse Melanoma (B16F10) Cell Growth

CellBio ◽

10.4236/cellbio.2014.32007 ◽

2014 ◽

Vol 03 (02) ◽

pp. 60-71 ◽

Cited By ~ 7

Author(s):

Pandurangan Ramaraj ◽

James L. Cox

Keyword(s):

Cell Growth ◽

Sex Steroids ◽

B16f10 Cell ◽

Mouse Melanoma ◽

Melanoma B16f10

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Eribulin activity in soft tissue sarcoma monolayer and three-dimensional cell line models: could the combination with other drugs improve its antitumoral effect?

Cancer Cell International ◽

10.1186/s12935-021-02337-5 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Javier Escudero ◽

Victoria Heredia-Soto ◽

Yinyin Wang ◽

Patricia Ruiz ◽

Yingying Hu ◽

...

Keyword(s):

Soft Tissue ◽

Cell Line ◽

Antitumour Activity ◽

Three Dimensional ◽

Soft Tissue Sarcomas ◽

Migration And Invasion ◽

2D And 3D ◽

First Time ◽

Dimensional Cell

Abstract Background Eribulin has shown antitumour activity in some soft tissue sarcomas (STSs), but it has only been approved for advanced liposarcoma (LPS). Methods In this study, we evaluated the effect of eribulin on proliferation, migration and invasion capabilities in LPS, leiomyosarcoma (LMS) and fibrosarcoma (FS) models, using both monolayer (2D) and three-dimensional (3D) spheroid cell cultures. Additionally, we explored combinations of eribulin with other drugs commonly used in the treatment of STS with the aim of increasing its antitumour activity. Results Eribulin showed activity inhibiting proliferation, 2D and 3D migration and invasion in most of the cell line models. Furthermore, we provide data that suggest, for the first time, a synergistic effect with ifosfamide in all models, and with pazopanib in LMS as well as in myxoid and pleomorphic LPS. Conclusions Our results support the effect of eribulin on LPS, LMS and FS cell line models. The combination of eribulin with ifosfamide or pazopanib has shown in vitro synergy, which warrants further clinical research.

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The Expression And The Tumor Suppressor Role of CLDN6 In Colon Cancer

10.21203/rs.3.rs-1090058/v1 ◽

2021 ◽

Author(s):

Huinan Qu ◽

Min Wang ◽

Miaomiao Wang ◽

Yuanyuan Liu ◽

Chengshi Quan

Keyword(s):

Colon Cancer ◽

Tumor Suppressor ◽

Cell Line ◽

Cancer Cell Line ◽

Human Colon ◽

Colon Cancer Cell Line ◽

Migration And Invasion ◽

Epithelial Cell Line

Abstract As a member of the tight junction family, CLDN6 is a tumor suppressor gene in breast cancer, but its role in colon cancer is unknown. In this research, we aimed at revealing the function of CLDN6 in colon cancer. We found that CLDN6 expressed lower in colon cancer tissues compared with adjacent normal tissues and the low expression of CLDN6 was correlated with lymph node metastasis. Similarly, CLDN6 expressed lower in the colon cancer cell line SW1116 compared with the normal human colon epithelial cell line NCM460. Upon CLDN6 overexpression in SW1116 cells, the proliferation of cells was suppressed in vitro and in vivo. Consistently, the migration and invasion abilities of cells were significantly inhibited after CLDN6 overexpression. Furthermore, the TYK2/STAT3 pathway was activated in SW1116/CLDN6 cells, and inhibition of this pathway with AG490 reversed the inhibition of migration and invasion of SW1116 cells by CLDN6. Therefore, our data indicated that CLDN6 acted as a tumor suppressor and had the potential to be regarded as a biomarker for the progression of colon cancer.

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microRNA-16 via Twist1 inhibits EMT induced by PM2.5 exposure in human hepatocellular carcinoma

Open Medicine ◽

10.1515/med-2019-0078 ◽

2019 ◽

Vol 14 (1) ◽

pp. 673-682 ◽

Cited By ~ 4

Author(s):

Hao Zhang ◽

Zhihu Li

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Line ◽

Epithelial Mesenchymal Transition ◽

Smooth Muscle Actin ◽

Human Hepatocellular Carcinoma ◽

Luciferase Reporter ◽

Migration And Invasion ◽

Dependent Manner ◽

Mesenchymal Transition

AbstractEpidemiological study has confirmed that PM2.5 (particulate matter with an aerodynamic diameter less than 2.5 μm) is associated with the incidence and progression of human hepatocellular carcinoma (HCC). Accordingly, this study was undertaken to investigate the pro-metastatic effects of PM2.5 on human HCC cell line SMMC-7721 in vitro and to explore the underlying mechanisms. CCK-8 assay was performed to examine the effect of PM2.5 on the proliferation of SMMC-7721 cells; scratch wound assay and transwell matrigel system has been used to examine the effect of PM2.5 on the migration and invasion ability of SMMC-7721 cells; furthermore, effect of PM2.5 on epithelial mesenchymal transition (EMT) of SMMC-7721 cells were examined by examining the EMT markers vimentin, ɑ-smooth muscle actin (ɑ-SMA), and E-cadherin; furthermore, the roles of microRNA-16 (miR-16) and its target Twist1 in PM2.5 induced carcinogenic effects were also examined. Results of CCK-8 assay suggested that PM2.5 promoted the proliferation of SMMC-7721 cells in a dose and time dependent manner. PM2.5 also markedly promoted the migration and invasion ability of SMMC-7721 cells. Moreover, epithelial mesenchymal transition (EMT) was also triggered by PM2.5. On the other hand, microRNA-16 (miR-16) and its target Twist1 was found to be mediated by PM2.5, and miR-16 mimic could suppress the metastatic ability of SMMC-7721 cells exposure to PM2.5 via inversely regulating the expression of Twist1. Furthermore, dual Luciferase reporter assay confirmed the specifically binding of miR-16 to the predicted 3′-UTR of Twist1. The present study confirmed the pro-proliferative and pro-metastatic effect of PM2.5 on HCC cell line SMMC-7721. The possible mechanisms were EMT process induced by PM2.5 in SMMC-7721 cells, which was accompanied by a decrease in miR-16 and increase in Twist1 expression.

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TSLC1 inhibits bladder cancer cells proliferation and promotes apoptosis by targeting miR-125b

10.21203/rs.2.22221/v1 ◽

2020 ◽

Author(s):

Jun Zhu ◽

Rui Hu ◽

NingJing Ou ◽

Zhen Liang ◽

Wei Zhang ◽

...

Keyword(s):

Bladder Cancer ◽

Cell Line ◽

Migration And Invasion ◽

P53 Expression ◽

Transwell Migration Assay ◽

Migration Assay ◽

Bladder Cancer Cells ◽

Cancer Tissues ◽

The Relationship

Abstract Backgroud: The aim of this study was to investigate the relationship between the expression of tumor suppressor in lung cancer-1 (TSLC1) and miRNA-125b in bladder cancer (BC) pathogenesis. Methods: The expression of miRNA-125b,TSLC1 and p53 in BC cell line was detected by real-time quantitative RT-PCR (RT-qPCR) or western blot. Transwell migration assay was used in the in vitro migration and invison anssay. TSLC1 and p53 expression was evaluated by immunohistochemistric staining in bladder cancer tissues. Results: We showed that the expression of miRNA-125b was significantly decreased in BC cell line(T24) transfection of miR-125b inhibitor.Knockdown of miRNA-125b promoted the growth and metastasis of T24 cells,while overexpression of miRNA-125b had the opposite effects. Furthermore,TSLC1 was significantly positive correlated with miRNA-125b expression and negative correlated with p53 expression in T24 cells.TSLC1 transfection increased the expression of miRNA-125b,and inhibited BC cell migration and invasion in vitro,and promoted apoptosis. The expression of TSLC1 and p53 was opposite in bladder cancer tissues. Conclusions: Our data provided strong evidence that TSLC1 inhibited tumorigenesis and development of BC through up-regulating tumor-suppressive miRNA-125b.

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