Applicability of random cloning method to analyze microbial community in full-scale anaerobic digesters

2008 ◽  
Vol 106 (2) ◽  
pp. 134-140 ◽  
Author(s):  
Jihoon Cheon ◽  
Taira Hidaka ◽  
Shuichi Mori ◽  
Hiromoto Koshikawa ◽  
Hiroshi Tsuno
Keyword(s):  
Microbial Community ◽  
Full Scale ◽  
Anaerobic Digesters ◽  
Cloning Method

scholarly journals Microbiota of anaerobic digesters in a full-scale wastewater treatment plant

2017 ◽  
Vol 43 (3) ◽  
pp. 53-60 ◽  
Author(s):  
Piotr Świątczak ◽  
Agnieszka Cydzik-Kwiatkowska ◽  
Paulina Rusanowska
Keyword(s):  
Microbial Community ◽  
Sewage Sludge ◽  
Methane Production ◽  
Treatment Plant ◽  
Full Scale ◽  
Successful Implementation ◽  
Anaerobic Digesters ◽  
Syntrophic Bacteria ◽  
The Stability ◽  
Microbial Groups

AbstractAnaerobic digestion is an important technology for the bio-based economy. The stability of the process is crucial for its successful implementation and depends on the structure and functional stability of the microbial community. In this study, the total microbial community was analyzed during mesophilic fermentation of sewage sludge in full-scale digesters.The digesters operated at 34–35°C, and a mixture of primary and excess sludge at a ratio of 2:1 was added to the digesters at 550 m3/d, for a sludge load of 0.054 m3/(m3·d). The amount and composition of biogas were determined. The microbial structure of the biomass from the digesters was investigated with use of next-generation sequencing.The percentage of methanogens in the biomass reached 21%, resulting in high quality biogas (over 61% methane content). The abundance of syntrophic bacteria was 4.47%, and stable methane production occurred at a Methanomicrobia to Synergistia ratio of 4.6:1.0. The two most numerous genera of methanogens (about 11% total) wereMethanosaetaandMethanolinea, indicating that, at the low substrate loading in the digester, the acetoclastic and hydrogenotrophic paths of methane production were equally important. The high abundance of the orderBacteroidetes, including the classCytophagia(11.6% of all sequences), indicated the high potential of the biomass for efficient degradation of lignocellulitic substances, and for degradation of protein and amino acids to acetate and ammonia.This study sheds light on the ecology of microbial groups that are involved in mesophilic fermentation in mature, stably-performing microbiota in full-scale reactors fed with sewage sludge under low substrate loading.

scholarly journals The impact of immigration on microbial community composition in full-scale anaerobic digesters

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Rasmus H. Kirkegaard ◽  
Simon J. McIlroy ◽  
Jannie M. Kristensen ◽  
Marta Nierychlo ◽  
Søren M. Karst ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Composition ◽  
Microbial Community Composition ◽  
Full Scale ◽  
Anaerobic Digesters ◽  
The Impact

scholarly journals Identifying the abundant and active microorganisms common to full-scale anaerobic digesters

2017 ◽  
Author(s):  
Rasmus H. Kirkegaard ◽  
Simon J. McIlroy ◽  
Jannie M. Kristensen ◽  
Marta Nierychlo ◽  
Søren M. Karst ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Amplicon Sequencing ◽  
Full Scale ◽  
Rrna Gene ◽  
Anaerobic Digesters ◽  
Abundant Otus ◽  
Source Of Information

AbstractAnaerobic digestion is widely applied to treat organic waste at wastewater treatment plants. Characterisation of the underlying microbiology represents a source of information to develop strategies for improved operation. To this end, we investigated the microbial community composition of thirty-two full-scale digesters over a six-year period using 16S rRNA gene amplicon sequencing. Sampling of the sludge fed into these systems revealed that several of the most abundant populations were likely inactive and immigrating with the influent. This observation indicates that a failure to consider immigration will interfere with correlation analysis and give an inaccurate picture of the active microbial community. Furthermore, several abundant OTUs could not be classified to genus level with commonly applied taxonomies, making inference of their function unreliable. As such, the existing MiDAS taxonomy was updated to include these abundant phylotypes. The communities of individual plants surveyed were remarkably similar – with only 300 OTUs representing 80% of the total reads across all plants, and 15% of these identified as likely inactive immigrating microbes. By identifying the abundant and active taxa in anaerobic digestion, this study paves the way for targeted characterisation of the process important organisms towards an in-depth understanding of the microbial ecology of these biotechnologically important systems.

scholarly journals Relationship between microbial activity and microbial community structure in six full-scale anaerobic digesters

2012 ◽  
Vol 167 (10) ◽  
pp. 581-589 ◽  
Author(s):  
Leticia Regueiro ◽  
Patricia Veiga ◽  
Mónica Figueroa ◽  
Jorge Alonso-Gutierrez ◽  
Alfons J.M. Stams ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Activity ◽  
Microbial Community Structure ◽  
Full Scale ◽  
Anaerobic Digesters

A time-course analysis of four full-scale anaerobic digesters in relation to the dynamics of change of their microbial communities

2011 ◽  
Vol 63 (4) ◽  
pp. 769-775 ◽  
Author(s):  
B. F. G. Pycke ◽  
C. Etchebehere ◽  
P. Van de Caveye ◽  
A. Negroni ◽  
W. Verstraete ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Time Course ◽  
Community Organization ◽  
Archaeal Community ◽  
Full Scale ◽  
Rate Of Change ◽  
Uasb Reactor ◽  
Rrna Gene ◽  
Anaerobic Digesters

This study describes the microbial community richness, -dynamics, and -organization of four full-scale anaerobic digesters during a time-course study of 45 days. The microbial community was analyzed using a Bacteria- and Archaea-targeting 16S rRNA gene-based Terminal-Restriction Fragment Length Polymorphism approach. Clustering analysis separated meso- and thermophilic reactors for both archaeal and bacterial communities. Regardless of the operating temperature, each installation possessed a distinct community profile. For both microbial domains, about 8 dominant terminal-restriction fragments could be observed, with a minimum of 4 and a maximum of 14. The bacterial community organization (a coefficient which describes the specific degree of evenness) showed a factor 2 more variation in the mesophilic reactors, compared with the thermophilic ones. The archaeal community structure of the mesophilic UASB reactor was found to be more stable. The community composition was highly dynamic for Bacteria and Archaea, with a rate of change between 20–50% per 15 days. This study illustrated that microbial communities in full-scale anaerobic digesters are unique to the installation and that community properties are dynamic. Converging complex microbial processes such as anaerobic digestion which rely on a multitude of microbial teams apparently can be highly dynamic.

scholarly journals Illumina sequencing of 16S rRNA genes reveals a unique microbial community in three anaerobic sludge digesters of Dubai

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249023
Author(s):  
Munawwar Ali Khan ◽  
Shams Tabrez Khan ◽  
Milred Cedric Sequeira ◽  
Sultan Mohammad Faheem ◽  
Naushad Rais
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Microbial Communities ◽  
Illumina Sequencing ◽  
Full Scale ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Gulf Region ◽  
Anaerobic Digesters ◽  
Fermentative Bacteria

Understanding the microbial communities in anaerobic digesters, especially bacteria and archaea, is key to its better operation and regulation. Microbial communities in the anaerobic digesters of the Gulf region where climatic conditions and other factors may impact the incoming feed are not documented. Therefore, Archaeal and Bacterial communities of three full-scale anaerobic digesters, namely AD1, AD3, and AD5 of the Jebel Ali Sewage water Treatment Plant (JASTP) were analyzed by Illumina sequencing of 16S rRNA genes. Among bacteria, the most abundant genus was fermentative bacteria Acetobacteroides (Blvii28). Other predominant bacterial genera in the digesters included thermophilic bacteria (Fervidobacterium and Coprothermobacter) and halophilic bacteria like Haloterrigena and Sediminibacter. This can be correlated with the climatic condition in Dubai, where the bacteria in the incoming feed may be thermophilic or halophilic as much of the water used in the country is desalinated seawater. The predominant Archaea include mainly the members of the phyla Euryarchaeota and Crenarchaeota belonging to the genus Methanocorpusculum, Metallosphaera, Methanocella, and Methanococcus. The highest population of Methanocorpusculum (more than 50% of total Archaea), and other hydrogenotrophic archaea, is in agreement with the high population of bacterial genera Acetobacteroides (Blvii28) and Fervidobacterium, capable of fermenting organic substrates into acetate and H2. Coprothermobacter, which is known to improve protein degradation by establishing syntrophy with hydrogenotrophic archaea, is also one of the digesters’ dominant genera. The results suggest that the microbial community in three full-scale anaerobic digesters is different. To best of our knowledge this is the first detailed report from the UAE.

A comparative evaluation of community structure in full-scale digesters indicates that two-phase digesters exhibit greater microbial diversity than single-phase digesters

2017 ◽  
Vol 3 (2) ◽  
pp. 304-311 ◽  
Author(s):  
A. L. Smith ◽  
T. Shimada ◽  
L. Raskin
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Comparative Evaluation ◽  
Single Phase ◽  
Full Scale ◽  
Community Diversity ◽  
Process Performance ◽  
Two Phase ◽  
Anaerobic Digesters ◽  
Microbial Community Diversity

Sequencing of full-scale, two-phase anaerobic digesters suggested that increased microbial community diversity improves process performance.

scholarly journals Anaerobic Digestion and Removal of Sulfamethoxazole, Enrofloxacin, Ciprofloxacin and Their Antibiotic Resistance Genes in a Full-Scale Biogas Plant

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 502
Author(s):  
Andrea Visca ◽  
Anna Barra Caracciolo ◽  
Paola Grenni ◽  
Luisa Patrolecco ◽  
Jasmin Rauseo ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Anaerobic Digestion ◽  
Resistance Genes ◽  
Emerging Contaminants ◽  
Antibiotic Resistance Genes ◽  
Animal Manure ◽  
Full Scale ◽  
Antibiotic Residues ◽  
Anaerobic Digesters ◽  
One Year

Anaerobic digestion is one of the best ways to re-use animal manure and agricultural residues, through the production of combustible biogas and digestate. However, the use of antibiotics for preventing and treating animal diseases and, consequently, their residual concentrations in manure, could introduce them into anaerobic digesters. If the digestate is applied as a soil fertilizer, antibiotic residues and/or their corresponding antibiotic resistance genes (ARGs) could reach soil ecosystems. This work investigated three common soil emerging contaminants, i.e., sulfamethoxazole (SMX), ciprofloxacin (CIP), enrofloxacin (ENR), their ARGs sul1, sul2, qnrS, qepA, aac-(6′)-Ib-cr and the mobile genetic element intI1, for one year in a full scale anaerobic plant. Six samplings were performed in line with the 45-day hydraulic retention time (HRT) of the anaerobic plant, by collecting input and output samples. The overall results show both antibiotics and ARGs decreased during the anaerobic digestion process. In particular, SMX was degraded by up to 100%, ENR up to 84% and CIP up to 92%, depending on the sampling time. In a similar way, all ARGs declined significantly (up to 80%) in the digestate samples. This work shows how anaerobic digestion can be a promising practice for lowering antibiotic residues and ARGs in soil.

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