scholarly journals Expression and Clinical Significance of NOD-Like Receptor Protein 3 (NLRP3) and Caspase-1 in Fetal Membrane and Placental Tissues of Patients with Premature Rupture of Membrane

2018 ◽  
Vol 24 ◽  
pp. 1560-1566 ◽  
Author(s):  
Jinming Zhu ◽  
Miao He ◽  
Chunling Ma ◽  
Fengyun Peng ◽  
Yuli Su ◽  
...  
Keyword(s):  
Clinical Significance ◽  
Receptor Protein ◽  
Fetal Membrane ◽  
Premature Rupture ◽  
Caspase 1

Does preconditioning lower the rupture resistance of chorioamniotic membrane?

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Arash Dadkhah ◽  
Ata Hashemi
Keyword(s):  
Mechanical Properties ◽  
Cesarean Delivery ◽  
Fetal Membrane ◽  
Premature Rupture ◽  
Rupture Stress ◽  
Probe Diameter ◽  
Healthy Pregnancy ◽  
Probe Size ◽  
Puncture Force ◽  
The Mean

AbstractPremature rupture of fetal membrane occurs in about 3% of all pregnancies. The physical integrity of chorioamnion (CA) membrane should be retained until delivery for a healthy pregnancy. To explore the effect of pre-conditioning and probe size on the mechanical properties of human chorioamniotic sac, the mechanical properties of 17 human chorioamniotic membranes, collected from cesarean delivery, were examined using biaxial puncture tests with and without preconditioning by different probe sizes. For preconditioned samples, the mean ± std. of ultimate rupture stress was calculated to be 1.73 ± 0.13, 1.61 ± 0.29 and 1.78 ± 0.26 MPa for the probe sizes of 3, 5 and 7 mm, respectively. For samples with no preconditioning, these values were calculated to be 2.38 ± 0.29, 2.36 ± 0.37, and 2.59 ± 0.43 MPa for the above-mentioned probe sizes. The force to probe diameter for samples with no preconditioning was in the range of 1087–1301 N/m for the three probe diameters, well in the range of 850–1580 N/m reported by previous studies. Our results show that the preconditioned samples had significantly lower ultimate puncture force and ultimate stress compared to non-preconditioned samples. In addition, a correlation between the probe size and the magnitude of puncture force was observed, while the stress values were not significantly affected by changing probe size.

Electroacupuncture inhibits the corneal ROS/TXNIP/NLRP3 signaling pathway in a rat model of dry eye syndrome

2021 ◽  
pp. 096452842110392
Author(s):  
Yanting Yang ◽  
Dan Zhang ◽  
Lijie Wu ◽  
Ji Zhang ◽  
Danyan Wu ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Dry Eye ◽  
Clinical Symptoms ◽  
Dry Eye Syndrome ◽  
Receptor Protein ◽  
Sprague Dawley ◽  
Corneal Injury ◽  
Caspase 1 ◽  
Des Model

Background: Electroacupuncture (EA) treatment has been found to ameliorate clinical symptoms in patients with dry eye, but its mechanisms are still not entirely clear. Objective: To study the regulation of EA on ocular surface function and the corneal reactive oxygen species (ROS)/thioredoxin-interacting protein (TXNIP)/Nod-like receptor protein 3 (NLRP3) inflammatory signaling pathway in dry eye syndrome (DES) model rats. Methods: Male Sprague-Dawley (SD) rats were randomly divided into five groups: Normal, Model, Model + EA, Model + NAC (N-actetylcysteine) and Model + NS (normal saline). The DES model was developed by subcutaneous injection of scopolamine hydrobromide with exposure to an air draft in the latter four groups. After intervention, the Schirmer I test (SIT), tear film break-up time (BUT) and ROS content were measured, the histopathological changes of corneal tissues were observed, and the mRNA and protein expression levels of TXNIP, NLRP3, apoptosis-associated Speck-like protein containing CARD (ASC), caspase-1, interleukin (IL)-1β and IL-18 were detected. Results: Compared with the Model group, the SIT and BUT increased significantly in the Model + EA group after intervention (p < 0.05), and the corneal injury was improved. Corneal ROS content declined in both Model + EA and Model + NAC groups (p < 0.05), and mRNA expression of TXNIP, NLRP3, ASC and caspase-1 also decreased (p < 0.01). Corneal protein expression of TXNIP, NLRP3, IL-1β and IL-18 decreased significantly in the Model + EA group (p < 0.01). Conclusion: Inhibiting the ROS/TXNIP/NLRP3 signaling pathway may be the mechanism underlying the role of EA in improving corneal injury in DES model rats.

scholarly journals Hubungan Ketuban Pecah dengan Kejadian Asfiksia Neonatorum di RSUD Kabupaten Kediri Tahun 2016

2017 ◽  
Vol 3 (2) ◽  
pp. 19
Author(s):  
Yanu Yufita Lestariningsih
Keyword(s):  
Public Hospital ◽  
Simple Random Sampling ◽  
Fetal Membrane ◽  
P Value ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Chi Square ◽  
Asphyxia Neonatorum ◽  
Chi Square Test ◽  
Almost All

Premature rupture of  membranerevolves 5-10% from all birth. Impact a birth premature that espoused broken fetal membrane early causess 12-15% asphyxia neonatorum. The purpose of the study to determine the correlation between premature rupture of  membrane with the incidence of asphyxia neonatorum. The research method used is analytic with retrospective cohort approach with independent variable of premature rupture of membrane, dependent variable asphyxia neonatorum. The study was conducted on 7 June to 12 July 2017. The population of 1519 mothers inregional public hospital of Kediri regency 2016. Sample 139 respondents, taken by simple random sampling, was analyzed by Chi Square test with a significant of 0.05. Result of research most of respondent with rupture of membrane counted 46 respondents, almost all respondents that is 82.6% gave birth baby with asphyxia. From result of Chi Square test obtained by result of p-value equal to 0.000 (<0.05), result of coefficient of contingency (C) equal to 0.639 with closeness strong relation, so H1 received H0 rejected which means there is significant relation with closeness strong between premature rupture of membrane with asphyxia neonatorum inregional public hospital of Kediri regency 2016. Relative Risk (RR) 1.65 which means the possibility of a baby experiencing asphyxia neonatorum of 1.65 times in maternal who experience premature rupture of membranes compared to mothers who did not experience premature rupture of membranes.

Abstract 254: Inflammasome Activity Is Essential For Deoxycorticosterone Acetate/salt-induced Hypertension In Mice

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Shalini M Krishnan ◽  
Christopher G Sobey ◽  
Barbara Kemp-Harper ◽  
Christopher T Chan ◽  
Henry Diep ◽  
...  
Keyword(s):  
Drinking Water ◽  
Receptor Protein ◽  
Salt Treatment ◽  
Deoxycorticosterone Acetate ◽  
Renal Inflammation ◽  
Protein Levels ◽  
Caspase 1 ◽  
Induced Hypertension ◽  
The Impact ◽  
Inflammasome Activity

Inflammasomes are signalling complexes comprised of a NOD-like receptor protein (NLRP), an adapter protein (ASC) and caspase-1. Inflammasomes detect host-derived danger signals and induce inflammation via activation of caspase-1, which in turn cleaves the cytokines pro-interleukin(IL)-1β and pro-IL-18 into their active, pro-inflammatory forms. Hypertension is associated with chronic renal inflammation, but the role of the inflammasome in this process is not known. Hence, we tested whether deoxycorticosterone acetate (DOCA)/salt-induced hypertension in mice is associated with increased expression and/or activation of the inflammasome in the kidney, and assessed the impact of inhibition of inflammasome activity on blood pressure (BP) and markers of renal inflammation and fibrosis. Male C57BL6/J (wild type) and ASC -/- mice were uninephrectomised, implanted with a DOCA pellet (2.4 mg/d, 21 d, s.c. ) and had their drinking water replaced with 1% saline (1K/DOCA/salt). Control mice had a kidney removed but received a placebo pellet and normal drinking water. 1K/DOCA/salt-treated mice had elevated systolic BP (146±4 mmHg) compared to control mice (115±2 mmHg; n=13-16; P<0.05). 1K/DOCA/salt-induced hypertension was associated with increased renal mRNA expression (fold-change vs control; n=7-9; P<0.05) of inflammasome subunits NLRP3 (2.3±0.2), ASC (2.8±0.6) and pro-caspase-1 (2.6±0.5), and the cytokine, pro-IL-1 (4.0±0.8). Moreover, protein levels of cleaved (active) caspase-1 and IL-1 were increased by 1.6±0.2- and 2.1±0.3-fold, respectively in kidneys of 1K/DOCA/salt vs control mice (n=6; P<0.05). ASC -/- mice, which lack an active inflammasome complex, displayed blunted hypertensive responses to 1K/DOCA/salt-treatment (140±3 mmHg) compared to wild types (155±8 mmHg; n=8-9; P<0.05). ASC -/- mice were also protected from 1K/DOCA/salt-induced increases in renal expression of the inflammatory genes IL-6, IL-17a, CCL2, ICAM-1 and VCAM-1, and accumulation of collagen. Thus, renal inflammation, fibrosis and elevated BP in response to 1K/DOCA/salt-treatment are critically dependent on inflammasome activity, highlighting this signalling complex and its cytokine products as potential therapeutic targets to treat hypertension.

scholarly journals Exosomes Mediate Hippocampal and Cortical Neuronal Injury Induced by Hepatic Ischemia-Reperfusion Injury through Activating Pyroptosis in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Limei Zhang ◽  
Hanyu Liu ◽  
Lili Jia ◽  
Jingshu Lyu ◽  
Ying Sun ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Neuronal Injury ◽  
Male Rats ◽  
Receptor Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatic Ischemia ◽  
Caspase 1 ◽  
Hepatic Ischemia Reperfusion

Background. The neuronal injury and cognitive dysfunction after liver transplantation have severe effects on the prognosis and life quality of patients. Accumulating evidence suggests that both exosomes and pyroptosis could participate in hepatic ischemia-reperfusion injury (HIRI) and play key roles in neuronal death. However, the link between exosomes and neuronal pyroptosis in HIRI awaits further investigation. Methods. After establishing the HIRI rat models, we primarily studied the role of pyroptosis in hippocampal and cortical neuron injury through detecting NOD-like receptor protein 3 (NLRP3), pro-caspase-1, cleaved-caspase-1, apoptosis-associated speck-like protein containing CARD (ASC), gasdermin D (GSDMD), interleukin-1beta (IL-1β), and interleukin-18 (IL-18) expressions with western blotting, immunohistochemical staining, and enzyme-linked immunosorbent assay (ELISA). Then, we intravenously injected normal male rats with exosomes isolated from the sera of HIRI-challenged rats and pretreated rats with MCC950, a specific inhibitor of NLRP3, and carried out the same assay. We also detected the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) in the hippocampal and cortical tissues. Results. The results indicated that NLRP3 inflammasome and caspase-1-dependent pyroptosis were activated in the hippocampus and cortex of HIRI rats. Furthermore, serum-derived exosomes from HIRI-challenged rats not only had the ability to cross the blood-brain barrier (BBB) but also had the similar effects on neuronal pyroptosis. Moreover, ROS and MDA productions were induced in the HIRI and exosome-challenged groups. In addition, to some degree, MCC950 could alleviate HIRI-mediated hippocampal and cortical neuronal pyroptosis. Conclusion. This study experimentally demonstrated that circulating exosomes play a critical role in HIRI-mediated hippocampal and cortical injury through regulating neuronal pyroptosis.

scholarly journals Distinct expression profile and histological distribution of NLRP3 inflammasome components in the tissues of Hainan black goat suggest a site-specific role in the inflammatory response

2017 ◽  
Vol 65 (3) ◽  
pp. 402-416 ◽  
Author(s):  
Kaizhao Zhang ◽  
Pan Tao ◽  
Jianxin Liu ◽  
Qingnan Wang ◽  
Shikun Ge ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Nlrp3 Inflammasome ◽  
Cortical Neurons ◽  
Inflammatory Diseases ◽  
Polyclonal Antibodies ◽  
Receptor Protein ◽  
Mesenteric Lymph Nodes ◽  
Specific Expression ◽  
Caspase 1 ◽  
Black Goat

The NOD-like receptor protein 3 (NLRP3) inflammasome comprised of NLRP3, ASC and caspase-1 plays an important role in the inflammatory and innate immune response. However, little is known about the expression pattern and histological distribution of these genes in goat. Here, we first cloned the fulllength cDNAs of the NLRP3, ASC and caspase-1 genes of Hainan black goat and produced their polyclonal antibodies. Tissue-specific expression and histological distribution of these genes were analysed. Phylogenetic analysis revealed that these three goat genes had high homology with Bos taurus genes and low homology with avian or fish genes. After immunisations with these recombinant Histagged proteins, the titres of antiserum were higher than 1:1024 and purified IgG was obtained. These three genes were expressed in all examined tissues, the mRNA expression level of NLRP3 and caspase-1 was most abundant in the spleen and mesenteric lymph nodes (MLNs), while ASC was primary expressed in the liver, spleen and kidney. The histological distribution of NLRP3, ASC and caspase-1 was detected in myocardial cells, hepatocytes, focal lymphocytes, bronchiolar epithelial cells, renal tubular epithelial cells, cortical neurons and endothelial cells of the germinal centres in the MLNs. These results will be helpful in further investigations into the function of the NLRP3 inflammasome and in elucidating its role in caprine inflammatory diseases.

Clinical significance of intra-amniotic inflammation in patients with preterm premature rupture of membranes

2004 ◽  
Vol 191 (4) ◽  
pp. 1339-1345 ◽  
Author(s):  
Soon-Sup Shim ◽  
Roberto Romero ◽  
Joon-Seok Hong ◽  
Chan-Wook Park ◽  
Jong Kwan Jun ◽  
...  
Keyword(s):  
Clinical Significance ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Preterm Premature Rupture

11: Fetal membrane microbiota is altered in preterm premature rupture of membranes

2014 ◽  
Vol 210 (1) ◽  
pp. S8 ◽  
Author(s):  
Amy Murtha ◽  
Kimberly Fortner ◽  
Brian Antczak ◽  
Jennifer Thompson ◽  
R. Phillips Heine ◽  
...  
Keyword(s):  
Fetal Membrane ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Preterm Premature Rupture

scholarly journals A study of perinatal outcome in preterm premature rupture of membranes

2018 ◽  
Vol 7 (12) ◽  
pp. 5061
Author(s):  
Poovathi M. ◽  
Yogalaksmi Yogalaksmi
Keyword(s):  
Preterm Birth ◽  
Perinatal Outcome ◽  
Health Centre ◽  
Late Preterm ◽  
Morbidity And Mortality ◽  
Fetal Membrane ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Preterm Premature Rupture ◽  
Preterm Group

Background: Preterm premature rupture of membranes is defined as rupture of fetal membrane before onset of labour at less than 37 completed weeks of gestation and after 28 weeks of gestation. Incidence ranges from 3-10% of all deliveries. Preterm premature rupture of membrane is one of the important causes of preterm birth can result inhigh perinatal morbidity and mortality. Preterm premature rupture of membranes complicates 3% of pregnancies and leads to one third of preterm birth. Preterm delivery affects one in 10 birth in USA and even greater birth in developing continues and causes 40-75% neonatal death. There are numerous risk factors for preterm premature rupture of membrane such as maternal, socioeconomic class, infection at early gestational age and associated co-morbid condition. Both mother and fetus are at greater risk of infection after preterm premature rupture of membrane.The fetal and neonatal morbidity and mortality risks are significantly affected by severity of oligohydrominos, duration of latency and gestation at preterm premature rupture of membrane. The objective is to study perinatal outcome in preterm premature rupture of membrane.Methods: This is a prospective study conducted in Mahathma Gandhi Memorial Government Hospital attached to K. A. P. V. Government Medical College, Trichy, Tamil Nadu, India. This is a tertiary health centre. This study has been conducted from January 2018 to June 2018.Results: Incidence of PPROM ranges from 3.0-10.0% of all deliveries. PPROM complicates approximately 3% of pregnancies and leads to one third of preterm birth.Conclusions: In present study most of newborn had better 5min Apgar especially late preterm group. In present study RDS was common in early preterm group and hyper bilirubinaemia common in late preterm group. In current study most of patients delivered vaginally compared to 36% of LSCS.

Sign in / Sign up

Export Citation Format

Share Document