Field trial on the efficacy of a new fenbendazole/praziquantel combination against nematodes and cestodes of dogs

The gastrointestinal parasitism of dogs and cats due to nematodes and cestodes is particularly common. In most cases animals are infected with both groups of parasites. The control of such mixed infections is carried out with nematocidal and cestocidal drugs administered either separately or combined. The aim of this study was to investigate the efficacy of a new fenbendazole/praziquantel (CANIQUANTEL PLUS®) combination against nematodes and cestodes of dogs. Toward this end 30 naturally infected dogs were divided into 2 groups: one (n=20) was treated with 50mg fenbendazole and 5mg praziquantel/kg b.w. for two consecutive days and the other (n=10) was not treated and served as the control group. Faecal examinations were carried out on the day of treatment as well as 10 days later. The efficacy level of this combined drug was found to be 98.2% and no adverse reactions were observed. It is concluded that, this anthelmintic combination may be safely used for the satisfactory control of mixed gastrointestinal parasitic infections in dogs.

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The Effects of Halofuginone on Wound Healing in the Rat Nasal Mucosa

American Journal of Rhinology and Allergy ◽

10.1177/1945892419866312 ◽

2019 ◽

Vol 34 (1) ◽

pp. 9-15 ◽

Cited By ~ 1

Author(s):

Seyit Mehmet Ceylan ◽

Erdal Uysal ◽

Mehmet Sokucu ◽

Efe Sezgin ◽

Mahmut Alper Kanmaz ◽

...

Keyword(s):

Wound Healing ◽

Nasal Mucosa ◽

Goblet Cell ◽

Cell Formation ◽

Treated Group ◽

Potential Effect ◽

The Other ◽

Parasitic Infections ◽

Control Group ◽

Cell Hyperplasia

Background Halofuginone is an alkaloid febrifugine analogue and bioactive molecule that was isolated incidentally from the Dichroa febrifuga plant. The therapeutic efficacy of halofuginone in parasitic infections, scleroderma, inflammation, and fibrosis-related diseases, as well as in some types of cancer, has been previously reported. The effects of halofuginone on nasal mucosal damage are not yet known. Objective The aim of this study was to investigate the potential effect of topically applied halofuginone on wound healing in the mechanically injured nasal mucosa of rats. Methods A unilateral mucosal wound was created in the nasal cavity of 32 rats (aged 4 weeks) using the brushing technique. These rats were randomly divided into 4 groups. Although the control group did not receive an intervention, a dry pad, a saline-impregnated pad, or a pad impregnated with halofuginone were placed in the rats of the other 3 groups and left for 5 minutes. Rats were sacrificed on the 14th day, and a histological examination was performed. The nasal mucosa was assessed via hematoxylin-eosin and Masson’s trichrome staining. Results There were no statistically significant differences in epithelial thickness, inflammation, goblet cell formation, and epithelial disarray values between the halofuginone group and the control group ( P > .05). The subepithelial thickness was significantly decreased in the saline-treated group and the halofuginone-treated group ( P < .05), but a significantly lower level of subepithelial fibrosis was only observed in the halofuginone group compared to the other groups ( P < .05). Conclusions Topical halofuginone administration reduces the development of fibrosis and subepithelial edema after experimentally induced nasal mucosal injury, but it does not exert therapeutic or preventive effects on epithelial damage, inflammation, and goblet cell hyperplasia.

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Adverse events when treating XDR tuberculosis

MedAlliance ◽

10.36422/23076348-2020-8-4-16-24 ◽

2020 ◽

Vol 8 (4) ◽

pp. 16-24

Keyword(s):

Clinical Study ◽

Adverse Events ◽

Adverse Reactions ◽

Main Group ◽

The Other ◽

Control Group ◽

Aminosalicylic Acid ◽

Control Groups ◽

Drug Induced ◽

And Control

The article presents the results of a clinical study on the frequency and spectrum of adverse events (AEs) that occur during treatment with an anti-tuberculosis drug (thioureidoiminomethylpyridinium perchlorate, Tpp). The purpose of the study was to determine the frequen-cy and nature of adverse events when TPP is included in the treatment regimen and to develop an algorithm for the prevention of adverse reactions. Materials and methods: 125 (N=125) patients with MDR and XDR tu-berculosis were examined. 70.4% (88) patients developed adverse events. Results and discussion: At the same time, adverse reactions occurred in 58.3% (35) of the main group, and in 69.2% (45) of the control group. There were no significant differences in the frequency of adverse events between the main and control groups (2=1.608, 95% CI 0.298-1.298, p>0.05). In the control group, diges-tive and hepatobiliary AEs were more frequent (up by 8.6% and 3.5%, respectively). On the other hand, derma-tological reactions prevailed in the main group (up by 3.8%). Tpp was not shown to be the main cause of drug induced hypothyroidism, it can jointly reinforce thyrotox-ic effect when used in combination with protionamide and/or PASA (para-aminosalicylic acid). Statistic analy-sis showed no significant differences in any class of AEs between the groups (p>0.05 for all AE categories). Up to 68% of adverse reactions were mild (1 and 2 grade). Con-clusion. Thioureidoiminomethylpyridinium perchlorate is not the main cause of drug induced hypothyroidism, it can jointly reinforce thyrotoxic effect when used in combina-tion with protionamide and/or PASA.

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Is halofantrine ototoxic? Experimental study on guinea pig cochlea model

The Journal of Laryngology & Otology ◽

10.1017/s0022215110001301 ◽

2010 ◽

Vol 124 (10) ◽

pp. 1061-1066

Author(s):

N M Iskander ◽

T F Youssef ◽

M R Ahmed ◽

A A K Mohamed

Keyword(s):

Hair Cells ◽

Therapeutic Dose ◽

Dose Group ◽

Adverse Reactions ◽

Outer Hair Cells ◽

The Other ◽

Control Group ◽

Group A ◽

Spiral Ganglia ◽

Ototoxic Drug

AbstractIntroduction:Halofantrine is a newly developed antimalarial drug used for the treatment of Plasmodium falciparum malaria. The introduction of this drug has been delayed because of its possible side effects, and due to insufficient studies on adverse reactions in humans. There have been no studies investigating its effect on hearing.Methods:Thirty guinea pigs were divided into three groups: a control group, a halofantrine therapeutic dose group and a halofantrine double therapeutic dose group. One cochlea specimen from each animal was stained with haematoxylin and eosin and the other with toluidine blue.Results:No changes were detected in the control group. The halofantrine therapeutic dose group showed loss and distortion of inner hair cells and inner phalangeal cells, and loss of spiral ganglia cells. In the halofantrine double therapeutic dose group, the inner and outer hair cells were distorted and there was loss of spiral ganglia cells.Conclusion:Halofantrine has mild to moderate pathological effects on cochlea histology, and can be considered an ototoxic drug.

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Effect of nalbuphine on rocuronium injection pain

World Journal of Pediatric Surgery ◽

10.1136/wjps-2020-000249 ◽

2021 ◽

Vol 4 (3) ◽

pp. e000249

Author(s):

Wenfang Huang ◽

Jinjin Huang ◽

Dongpi Wang ◽

Yaoqin Hu ◽

Jiangmei Wang ◽

...

Keyword(s):

Respiratory Depression ◽

Intravenous Injection ◽

Adverse Reactions ◽

The Other ◽

Injection Pain ◽

Control Group ◽

Lidocaine Group ◽

Group Control ◽

Group Control Group ◽

Control Group Group

ObjectiveRocuronium-associated intravenous injection pain occurs frequently in children during induction of anesthesia. The aim of this study was to systematically evaluate the benefits of nalbuphine in patients with rocuronium-associated injection pain.MethodsNinety children undergoing tonsillectomy and adenoidectomy in our hospital between October 2019 and September 2020 were randomly divided into the following groups, with 30 patients per group: control group (group C), lidocaine group (group L), and nalbuphine group (group N). Routine 0.1 mg/kg midazolam and 2 mg/kg propofol were injected intravenously. After sedation, children in group C, group L, and group N were administered an intravenous injection of saline, lidocaine (10 mg/mL), or nalbuphine hydrochloride (2 mg/mL), respectively, at a dosage of 0.1 mL/kg. Intravenous injection of rocuronium stock solution (0.6 mg/kg) was administered 2 minutes later. Pain was evaluated using Ambeshs 4-pointscale. The incidence of rocuronium injection pain was compared among the three groups, and postoperative adverse reactions, such as drowsiness, bradycardia, hypotension, and respiratory depression, were evaluated.ResultsThe incidence of injection pain among children in group N was significantly lower than that in group C and group L (p<0.05). The incidence of drowsiness in group N was significantly higher than that in the other groups (p<0.05). The incidences of hypotension, bradycardia, and respiratory depression were not significantly different among the three groups (p>0.05).ConclusionsIntravenous nalbuphine during induction of anesthesia effectively prevented rocuronium-associated injection pain in children. Drowsiness is a complication.

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Detection and Assessment of Interleukin 6 in Irreversible Pulp Inflamation

Revista de Chimie ◽

10.37358/rc.17.2.5445 ◽

2017 ◽

Vol 68 (2) ◽

pp. 323-327

Author(s):

Cristian Levente Giuroiu ◽

Maria Vataman ◽

Gabriel Melian ◽

Dragos Bularda ◽

Ludmila Lozneanu ◽

...

Keyword(s):

Dental Caries ◽

Interleukin 6 ◽

Dental Pulp ◽

The Other ◽

Control Group ◽

Study Group ◽

Pulp Tissue ◽

Immunohistochemical Technique ◽

Systemic Distribution ◽

Cell Zone

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis. The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.

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Antimicrobial Effect of 940 nm Diode Laser based on Photolysis of Hydrogen Peroxide in the Treatment of Periodontal Disease

Revista de Chimie ◽

10.37358/rc.18.8.6478 ◽

2018 ◽

Vol 69 (8) ◽

pp. 2081-2088 ◽

Cited By ~ 1

Author(s):

Alin Alexandru Odor ◽

Edwin Sever Bechir ◽

Deborah Violant ◽

Victoria Badea

Keyword(s):

Hydrogen Peroxide ◽

Laser Therapy ◽

Diode Laser ◽

Antimicrobial Effect ◽

The Other ◽

Control Group ◽

Group 4 ◽

Periodontal Bacteria ◽

Severe Periodontitis ◽

Before And After

Moderate and severe periodontitis represents a challenge in the non-surgical periodontal therapy. Due to the lack of evidence regarding the antimicrobial effectiveness of 940 nm diode laser in periodontal treatment, this study aimed to evaluate the antimicrobial effect of hydrogen peroxide (H2O2) photolysis performed with 940 nm diode laser in the treatment of moderate and severe periodontitis. Twenty-five patients with 100 teeth were selected for this pilot study. The test teeth were randomly assigned to one of the four treatment groups: Group 1: scaling and root planning (SRP) (control group); and the following experimental groups: Group 2: H2O2; Group 3: 940 nm diode laser therapy; Group 4: 940 nm diode laser therapy and H2O2. Clinical examinations, like probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were performed before and after the treatment. The microbiological evaluation, effectuated before and after the treatment, included nine periodontal bacteria species and investigated by means of real-time PCR assay. The clinical and bacterial differences in the tested groups, was assessed between control group and the other three experimental groups, as well as between the experimental groups. The total bacteria load was reduced for all four studied groups. Group 4 (diode laser + H2O2) showed significant bacterial reduction of the major periodontal bacteria like Pg., Tf., Td., Pi., Pm., Fn (p[0.001) than the other 3 groups (p]0.001). Also the periodontal clinical parameters, like PD, CAL and BOP showed a significant reduction after the photolysis of H2O2 with the 940 nm diode laser (p[0.001). Differences between tested groups showed a significant beneficial results in regard to Group 4.It is suggested that the photoactivation of H2O2 with the 940 nm diode laser can be used successfully in adjunctive to the non-surgical periodontal treatment as a bactericidal tool.

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Psycho-Electrophysiological Benefits of Forest Therapies Focused on Qigong and Walking with Elderly Individuals

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18063004 ◽

2021 ◽

Vol 18 (6) ◽

pp. 3004

Author(s):

Jiyune Yi ◽

Seul Gee Kim ◽

Taegyu Khil ◽

Minja Shin ◽

Jin-Hee You ◽

...

Keyword(s):

Urban Forest ◽

Nervous Activity ◽

Health Problems ◽

The Other ◽

Upper Body ◽

Sympathetic Nervous Activity ◽

Control Group ◽

Beta Band ◽

Elderly Individuals ◽

Beneficial Effects

We developed two distinct forest therapy programs (FTPs) and compared their effects on dementia prevention and related health problems for older adults. One was focused on Qigong practice in the forest (QP) and the other involved active walking in the forest (WP). Both FTPs consisted of twelve 2-h sessions over six weeks and were conducted in an urban forest. We obtained data from 25, 18, and 26 participants aged 65 years or above for the QP, WP, and control groups, respectively. Neuropsychological scores via cognition (MoCA), geriatric depression (GDS) and quality of life (EQ-5D), and electrophysiological variables (electroencephalography, bioimpedance, and heart rate variability) were measured. We analyzed the intervention effects with a generalized linear model. Compared to the control group, the WP group showed benefits in terms of neurocognition (increases in the MoCA score, and alpha and beta band power values in the electroencephalogram), sympathetic nervous activity, and bioimpedance in the lower body. On the other hand, the QP group showed alleviated depression and an increased bioimpedance phase angle in the upper body. In conclusion, both active walking and Qigong in the forest were shown to have distinctive neuropsychological and electrophysiological benefits, and both had beneficial effects in terms of preventing dementia and relieving related health problems for elderly individuals.

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Antioxidant activity of Lactobacillus plantarum NJAU-01 in an animal model of aging

BMC Microbiology ◽

10.1186/s12866-021-02248-5 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Qingfeng Ge ◽

Bo Yang ◽

Rui Liu ◽

Donglei Jiang ◽

Hai Yu ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Lactobacillus Plantarum ◽

Meat Product ◽

The Other ◽

Oxidative Stress Marker ◽

Control Group ◽

Intragastric Administration ◽

Aging Effects ◽

Sterile Saline

Abstract Background Excessive reactive oxygen species (ROS) can cause serious damage to the human body and may cause various chronic diseases. Studies have found that lactic acid bacteria (LAB) have antioxidant and anti-aging effects, and are important resources for the development of microbial antioxidants. This paper was to explore the potential role of an antioxidant strain, Lactobacillus plantarum NJAU-01 screened from traditional dry-cured meat product Jinhua Ham in regulating D-galactose-induced subacute senescence of mice. A total of 48 specific pathogen free Kun Ming mice (SPF KM mice) were randomly allocated into 6 groups: control group with sterile saline injection, aging group with subcutaneously injection of D-galactose, treatments groups with injection of D-galactose and intragastric administration of 107, 108, and 109 CFU/mL L. plantarum NJAU-01, and positive control group with injection of D-galactose and intragastric administration of 1 mg/mL Vitamin C. Results The results showed that the treatment group of L. plantarum NJAU-01 at 109 CFU/mL showed higher total antioxidant capacity (T-AOC) and the antioxidant enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) than those of the other groups in serum, heart and liver. In contrast, the content of the oxidative stress marker malondialdehyde (MDA) showed lower levels than the other groups (P < 0.05). The antioxidant capacity was improved with the supplement of the increasing concentration of L. plantarum NJAU-01. Conclusions Thus, this study demonstrates that L. plantarum NJAU-01 can alleviate oxidative stress by increasing the activities of enzymes involved in oxidation resistance and decreasing level of lipid oxidation in mice.

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The change of gut microbiota in MDD patients under SSRIs treatment

Scientific Reports ◽

10.1038/s41598-021-94481-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yang Shen ◽

Xiao Yang ◽

Gaofei Li ◽

Jiayu Gao ◽

Ying Liang

Keyword(s):

Gut Microbiota ◽

Maximum Dose ◽

Antidepressant Treatment ◽

Intestinal Flora ◽

Alpha Diversity ◽

The Other ◽

Control Group ◽

Depressed Patients ◽

Metabolic Functions

AbstractThe alterations in the gut microbiota have been reported to be correlated with the development of depression. The purpose of this study was to investigate the changes of intestinal microbiota in depressed patients after antidepressant treatment. We recruited 30 MDD patients (MDD group) and 30 healthy controls (control group). The MDD group received individualized treatment with escitalopram at a maximum dose of 20 mg/day. After depressive symptoms improved to a HAMD scale score > 50%, a fecal sample was collected again and used as the follow-up group. The differences of gut microbiota between patients and controls, the characteristics of gut microbiota under treatment and the potential differences in metabolic functions were thus investigated. The Firmicutes/Bacteroidetes ratio was significantly different within three groups, and the ratio of follow-up group was significantly lower than those of the other two groups. Alpha diversity was significantly higher in MDD group than those of the other groups, and the alpha diversity was not significantly different between control and follow-up groups. The beta diversity of some patients resembled participants in the control group. The metabolic function of gut microbiota after treatment was still different from that of the control group. This study suggests that the intestinal flora of depressed patients has a tendency to return to normal under escitalopram treatment.

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Ghrelin and orexin levels in infertile male: evaluation of effects on varicocele pathophysiology, relationship seminal and hormonal parameter

Turkish Journal of Biochemistry ◽

10.1515/tjb-2020-0084 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Üçler Kısa ◽

M. Murad Başar ◽

Timuçin Şipal ◽

Özlem Doğan Ceylan

Keyword(s):

The Other ◽

Control Group ◽

Infertile Male ◽

Seminal Parameters ◽

Other Hand ◽

Healthy Control ◽

Regulatory Effects ◽

Serum Ghrelin ◽

Infertile Patients ◽

Group 1

AbstractObjectiveThe aim of the present study was to investigate serum ghrelin and orexin levels in patients with varicocele and compare these levels with idiopathic infertile male and healthy control cases.MethodsThis study enrolled 24 men with varicocele, 24 males having idiopathic infertility, and 21 fertile men as the control group. Hormonal analyses, ghrelin and orexin levels were measured samples. Semen was analyzed after 3 and 5 days of sexual abstinence.ResultsSerum ghrelin levels were statistically different among the three groups (p=0.015), and it was due to a statistically lower level in group-1 than the level in the control cases (p=0.012). On the other hand, serum orexin levels were lower than healthy subjects in infertile groups with/without varicocele, but there was no difference (p=0.685) among three groups. Serum ghrelin level showed a negative and significant correlation only with sperm motility (r=−0.646, p=0.022), there was no correlation with other parameters. On the other hand, serum orexin levels did not show a significant correlation with seminal parameters.ConclusionBoth new investigated peptides ghrelin and orexin have regulatory effects on testicular function. However, ghrelin has a more obvious and complex effect on spermatogenesis. Impaired seminal parameters, especially motility was associated with increased serum ghrelin levels in infertile patients, especially with varicocele.

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